{"id":"https://openalex.org/W2036699032","doi":"https://doi.org/10.1074/jbc.m003469200","title":"Farnesyl Transferase Inhibitors Block the Farnesylation of CENP-E and CENP-F and Alter the Association of CENP-E with the Microtubules","display_name":"Farnesyl Transferase Inhibitors Block the Farnesylation of CENP-E and CENP-F and Alter the Association of CENP-E with the Microtubules","publication_year":2000,"publication_date":"2000-09-01","ids":{"openalex":"https://openalex.org/W2036699032","doi":"https://doi.org/10.1074/jbc.m003469200","mag":"2036699032","pmid":"https://pubmed.ncbi.nlm.nih.gov/10852915"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m003469200","doi":"https://doi.org/10.1074/jbc.m003469200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m003469200","pdf_url":"http://www.jbc.org/article/S0021925818443876/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925818443876/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021984885","display_name":"Hena R. Ashar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hena R. Ashar","raw_affiliation_strings":["Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033, USA","From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033, USA","institution_ids":[]},{"raw_affiliation_string":"From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045428966","display_name":"Linda J. James","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Linda James","raw_affiliation_strings":["From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103402135","display_name":"Kimberly Gray","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kimberly Gray","raw_affiliation_strings":["From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050801977","display_name":"Donna M. Carr","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Donna Carr","raw_affiliation_strings":["From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103572151","display_name":"Stuart Black","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Stuart Black","raw_affiliation_strings":["From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041186281","display_name":"Lydia Armstrong","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lydia Armstrong","raw_affiliation_strings":["From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035909919","display_name":"Walter Robert Bishop","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"W. Robert Bishop","raw_affiliation_strings":["From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5034758372","display_name":"Paul T. Kirschmeier","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Paul Kirschmeier","raw_affiliation_strings":["From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5034758372"],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":7.3723,"has_fulltext":true,"cited_by_count":317,"citation_normalized_percentile":{"value":0.98798662,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"275","issue":"39","first_page":"30451","last_page":"30457"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10492","display_name":"Microtubule and mitosis dynamics","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10492","display_name":"Microtubule and mitosis dynamics","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10583","display_name":"Cancer-related Molecular Pathways","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/farnesyltransferase-inhibitors","display_name":"farnesyltransferase inhibitors","score":0.9890000224113464},{"id":"https://openalex.org/keywords/cenp-e","display_name":"CENP-E","score":0.8889999985694885},{"id":"https://openalex.org/keywords/cenpf","display_name":"CENPF","score":1.0},{"id":"https://openalex.org/keywords/protein-farnesylation","display_name":"farnesylation","score":0.8220000267028809},{"id":"https://openalex.org/keywords/caax-box","display_name":"CAAX box","score":0.8740000128746033},{"id":"https://openalex.org/keywords/prenylation","display_name":"prenylation","score":0.8169999718666077},{"id":"https://openalex.org/keywords/kinetochore","display_name":"kinetochore","score":0.906000018119812},{"id":"https://openalex.org/keywords/g2-m-checkpoint","display_name":"G2/M checkpoint","score":0.8920000195503235},{"id":"https://openalex.org/keywords/mitosis","display_name":"mitosis","score":0.753000020980835},{"id":"https://openalex.org/keywords/geranylgeranyltransferase-i","display_name":"geranylgeranyltransferase I","score":0.8539999723434448},{"id":"https://openalex.org/keywords/centromeric-proteins","display_name":"centromeric proteins","score":0.6449999809265137},{"id":"https://openalex.org/keywords/cancer-cell-lines","display_name":"cancer cell lines","score":0.4950000047683716}],"concepts":[{"id":"https://openalex.org/C57790582","wikidata":"https://www.wikidata.org/wiki/Q901141","display_name":"Prenylation","level":3,"score":0.9141839742660522},{"id":"https://openalex.org/C2778396349","wikidata":"https://www.wikidata.org/wiki/Q410994","display_name":"Farnesyltransferase","level":4,"score":0.7499517798423767},{"id":"https://openalex.org/C93304396","wikidata":"https://www.wikidata.org/wiki/Q14763008","display_name":"Mitosis","level":2,"score":0.5878040194511414},{"id":"https://openalex.org/C2776376580","wikidata":"https://www.wikidata.org/wiki/Q407355","display_name":"Transferase","level":3,"score":0.5694465637207031},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5679134130477905},{"id":"https://openalex.org/C2777827897","wikidata":"https://www.wikidata.org/wiki/Q5436005","display_name":"Farnesyltransferase inhibitor","level":5,"score":0.4795546233654022},{"id":"https://openalex.org/C23167141","wikidata":"https://www.wikidata.org/wiki/Q14912965","display_name":"Geranylgeranylation","level":4,"score":0.4554675221443176},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4482637643814087},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.42266008257865906},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.41209733486175537},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3785858750343323},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3204662799835205},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2621677815914154},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.06799983978271484}],"mesh":[{"descriptor_ui":"D002453","descriptor_name":"Cell Cycle","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002868","descriptor_name":"Chromosomal Proteins, Non-Histone","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003986","descriptor_name":"Dibenzocycloheptenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004791","descriptor_name":"Enzyme Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008798","descriptor_name":"Mevalonic Acid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008840","descriptor_name":"Microfilament Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008869","descriptor_name":"Microtubule-Associated Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008870","descriptor_name":"Microtubules","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010880","descriptor_name":"Piperidines","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011725","descriptor_name":"Pyridines","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017368","descriptor_name":"Protein Prenylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019883","descriptor_name":"Alkyl and Aryl Transferases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m003469200","doi":"https://doi.org/10.1074/jbc.m003469200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m003469200","pdf_url":"http://www.jbc.org/article/S0021925818443876/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:10852915","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10852915","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m003469200","doi":"https://doi.org/10.1074/jbc.m003469200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m003469200","pdf_url":"http://www.jbc.org/article/S0021925818443876/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8048999905586243,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2036699032.pdf","grobid_xml":"https://content.openalex.org/works/W2036699032.grobid-xml"},"referenced_works_count":52,"referenced_works":["https://openalex.org/W44676527","https://openalex.org/W126176001","https://openalex.org/W140618674","https://openalex.org/W1488360842","https://openalex.org/W1503798699","https://openalex.org/W1507719842","https://openalex.org/W1516442290","https://openalex.org/W1526004337","https://openalex.org/W1544277307","https://openalex.org/W1564880184","https://openalex.org/W1587169392","https://openalex.org/W1594384581","https://openalex.org/W1594629389","https://openalex.org/W1970606222","https://openalex.org/W1975702431","https://openalex.org/W1985753931","https://openalex.org/W1993000462","https://openalex.org/W1998147517","https://openalex.org/W2017098460","https://openalex.org/W2017138782","https://openalex.org/W2018711267","https://openalex.org/W2022116509","https://openalex.org/W2022947433","https://openalex.org/W2026834760","https://openalex.org/W2032206809","https://openalex.org/W2036082581","https://openalex.org/W2036530157","https://openalex.org/W2037480365","https://openalex.org/W2045369188","https://openalex.org/W2051626784","https://openalex.org/W2067519095","https://openalex.org/W2079655986","https://openalex.org/W2080628130","https://openalex.org/W2083506343","https://openalex.org/W2091054428","https://openalex.org/W2099658600","https://openalex.org/W2100147739","https://openalex.org/W2107424534","https://openalex.org/W2108688550","https://openalex.org/W2111644689","https://openalex.org/W2117417095","https://openalex.org/W2120153758","https://openalex.org/W2126683450","https://openalex.org/W2136460759","https://openalex.org/W2145978772","https://openalex.org/W2150287123","https://openalex.org/W2156654096","https://openalex.org/W2167786295","https://openalex.org/W2255894651","https://openalex.org/W2416700216","https://openalex.org/W2416894777","https://openalex.org/W4301342824"],"related_works":["https://openalex.org/W1254790682","https://openalex.org/W2142919712","https://openalex.org/W2024210572","https://openalex.org/W2071517079","https://openalex.org/W2115119282","https://openalex.org/W2133196855","https://openalex.org/W2100359874","https://openalex.org/W2145978772","https://openalex.org/W2103726956","https://openalex.org/W2081911025"],"abstract_inverted_index":{"Human":[0],"tumor":[1,99],"cell":[2,100,121],"lines":[3],"that":[4,26,71,125,168],"are":[5,51,57,82,94],"sensitive":[6],"to":[7,147,192],"the":[8,45,62,74,97,109,118,127,136,148,151,158,163,169,176,179,187],"effects":[9],"of":[10,61,78,129,153,171,178,189],"farnesyl":[11,85,110,137,154],"transferase":[12,86,111,138,155],"inhibitors":[13,156],"accumulate":[14,27],"in":[15,28,96,175,186],"G(2)":[16,63],"-->":[17,64],"M":[18,65],"(except":[19],"for":[20,32,84],"cells":[21,190],"with":[22,117,135],"an":[23],"activated":[24],"Ha-ras":[25],"G(1)).":[29],"A":[30],"search":[31],"CAAX":[33,76],"box":[34,77],"proteins":[35,47,93],"from":[36,73],"Swiss-Prot":[37],"revealed":[38],"more":[39],"than":[40],"300":[41],"peptides.":[42],"Of":[43],"these,":[44],"centromeric":[46],"CENP-E":[48,79,130,161,172],"and":[49,56,80,131,162,184],"CENP-F":[50,81,132],"preferentially":[52],"expressed":[53],"during":[54,182],"mitosis":[55,183],"implicated":[58],"as":[59],"mediators":[60],"checkpoint.":[66],"Experiments":[67],"performed":[68],"here":[69],"show":[70],"peptides":[72],"COOH-terminal":[75],"substrates":[83],"but":[87],"not":[88,143],"geranylgeranyl":[89],"transferase-I.":[90],"Although":[91],"both":[92],"prenylated":[95],"human":[98],"line":[101],"DLD-1,":[102],"their":[103,145],"prenylation":[104],"is":[105],"completely":[106],"inhibited":[107],"by":[108,133],"inhibitor,":[112],"SCH":[113,140],"66336.":[114],"Immunohistochemical":[115],"data":[116],"lung":[119],"carcinoma":[120],"line,":[122],"A549,":[123],"showed":[124],"preventing":[126],"farnesylation":[128,173],"treatment":[134],"inhibitor":[139],"66336":[141],"does":[142],"affect":[144],"localization":[146],"kinetochores.":[149],"However,":[150],"presence":[152],"alters":[157],"association":[159],"between":[160],"microtubules.":[164],"Our":[165],"results":[166,174,185],"imply":[167],"inhibition":[170],"alteration":[177],"microtubule-centromere":[180],"interaction":[181],"accumulation":[188],"prior":[191],"metaphase.":[193]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}