{"id":"https://openalex.org/W2035541995","doi":"https://doi.org/10.1021/bi00587a005","title":"Structural basis for the anticoagulant activity of heparin. 2. Relationship of anticoagulant activity to the thermodynamics and fluorescence fading kinetics of acridine orange-heparin complexes","display_name":"Structural basis for the anticoagulant activity of heparin. 2. Relationship of anticoagulant activity to the thermodynamics and fluorescence fading kinetics of acridine orange-heparin complexes","publication_year":1979,"publication_date":"1979-10-02","ids":{"openalex":"https://openalex.org/W2035541995","doi":"https://doi.org/10.1021/bi00587a005","mag":"2035541995","pmid":"https://pubmed.ncbi.nlm.nih.gov/486425"},"language":"en","primary_location":{"id":"doi:10.1021/bi00587a005","doi":"https://doi.org/10.1021/bi00587a005","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00587a005","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056416150","display_name":"Julian M. Menter","orcid":"https://orcid.org/0000-0003-3524-7487","observed_orcids":["https://orcid.org/0000-0003-3524-7487"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Julian M. Menter","raw_affiliation_strings":["VA Medical Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VA Medical Center","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016405661","display_name":"Robert E. Hurst","orcid":"https://orcid.org/0000-0003-0370-1817","observed_orcids":["https://orcid.org/0000-0003-0370-1817"]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert E. Hurst","raw_affiliation_strings":["University of Alabama at, Birmingham"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Alabama at, Birmingham","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082577123","display_name":"DAVID A. CORLISS","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"David A. Corliss","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060224154","display_name":"Seymour S. West","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Seymour S. West","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5035978243","display_name":"Edwin W. Abrahamson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79817857","display_name":"University of Guelph","ror":"https://ror.org/01r7awg59","country_code":"CA","type":"education","lineage":["https://openalex.org/I79817857"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Edwin W. Abrahamson","raw_affiliation_strings":["University of Guelph\u2020"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Guelph\u2020","institution_ids":["https://openalex.org/I79817857"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.9155,"has_fulltext":false,"cited_by_count":14,"citation_normalized_percentile":{"value":0.74971004,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"18","issue":"20","first_page":"4288","last_page":"4292"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.9247999787330627,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.9247999787330627,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12274","display_name":"Electrostatics and Colloid Interactions","score":0.013199999928474426,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.012500000186264515,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heparin","display_name":"heparin","score":0.9750000238418579},{"id":"https://openalex.org/keywords/anticoagulant-activity","display_name":"anticoagulant activity","score":0.9309999942779541},{"id":"https://openalex.org/keywords/acridine-orange","display_name":"acridine orange","score":0.8679999709129333},{"id":"https://openalex.org/keywords/dermatan-sulfate","display_name":"dermatan sulfate","score":0.8790000081062317},{"id":"https://openalex.org/keywords/glycosaminoglycans","display_name":"glycosaminoglycans","score":0.9340000152587891},{"id":"https://openalex.org/keywords/entropy-of-activation","display_name":"activation entropy","score":0.675000011920929},{"id":"https://openalex.org/keywords/electrostatic-interactions","display_name":"electrostatic interactions","score":0.7170000076293945},{"id":"https://openalex.org/keywords/conformational-dynamics","display_name":"conformational dynamics","score":0.5899999737739563},{"id":"https://openalex.org/keywords/temperature-dependence","display_name":"temperature dependence","score":0.46299999952316284}],"concepts":[{"id":"https://openalex.org/C2779102032","wikidata":"https://www.wikidata.org/wiki/Q342717","display_name":"Acridine orange","level":3,"score":0.6204788088798523},{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.52849942445755},{"id":"https://openalex.org/C148898269","wikidata":"https://www.wikidata.org/wiki/Q1108792","display_name":"Kinetics","level":2,"score":0.4826836884021759},{"id":"https://openalex.org/C2778205648","wikidata":"https://www.wikidata.org/wiki/Q215118","display_name":"Anticoagulant","level":2,"score":0.4458683431148529},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.40312460064888},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.39040231704711914},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.28166431188583374},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.2675970792770386},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.2483130395412445},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.21772855520248413},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16156825423240662},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000165","descriptor_name":"Acridine Orange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000925","descriptor_name":"Anticoagulants","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003871","descriptor_name":"Dermatan Sulfate","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006493","descriptor_name":"Heparin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00587a005","doi":"https://doi.org/10.1021/bi00587a005","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00587a005","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:486425","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/486425","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":16,"referenced_works":["https://openalex.org/W9987655","https://openalex.org/W61185141","https://openalex.org/W207555618","https://openalex.org/W1891400877","https://openalex.org/W1933438817","https://openalex.org/W1997412617","https://openalex.org/W1998717980","https://openalex.org/W2016137730","https://openalex.org/W2016329557","https://openalex.org/W2022754381","https://openalex.org/W2027803537","https://openalex.org/W2027812955","https://openalex.org/W2049451223","https://openalex.org/W2092495092","https://openalex.org/W2136529911","https://openalex.org/W2418165386"],"related_works":["https://openalex.org/W2037642407","https://openalex.org/W2022778851","https://openalex.org/W2051273079","https://openalex.org/W2076762665","https://openalex.org/W1968026160","https://openalex.org/W2477400714","https://openalex.org/W2884199648","https://openalex.org/W2321554630","https://openalex.org/W2070345128","https://openalex.org/W2002675524"],"abstract_inverted_index":{"Complexing":[0],"heparin":[1,96],"or":[2],"dermatan":[3,87],"sulfate":[4,88],"with":[5,139],"the":[6,29,62,82,91,95,121,150],"fluorescent":[7],"probe":[8],"acridine":[9],"orange":[10],"provides":[11],"a":[12,125,146],"means":[13],"of":[14,25,36,52,109],"studying":[15],"electrostatic":[16],"as":[17,19],"well":[18],"static":[20],"and":[21,31,49,70,117,160],"dynamic":[22],"conformational":[23],"aspects":[24],"these":[26,37],"glycosaminoglycans":[27],"via":[28],"thermodynamic":[30],"photochemical":[32],"(fluorescence":[33],"fading)":[34],"properties":[35],"complexes.":[38],"The":[39,142],"cooperative":[40],"binding":[41],"constants":[42],"(Kq),":[43],"fluorescence":[44,133,158],"fading":[45,104,134,159],"rate":[46,105],"parameters":[47],"(r''),":[48],"anticoagulant":[50,75,140,161],"activities":[51],"heparins":[53,108],"fractionated":[54],"according":[55],"to":[56,123],"anionic":[57,66,111,154],"density":[58,155],"all":[59],"showed":[60],"qualitatively":[61],"same":[63],"dependence":[64],"upon":[65],"density.":[67],"When":[68],"Kq":[69],"r''":[71],"were":[72,79],"plotted":[73],"against":[74],"activity,":[76],"empirical":[77],"relationships":[78],"observed.":[80],"Interestingly,":[81],"corresponding":[83],"values":[84],"for":[85,107,132],"unfractionated":[86],"fell":[89],"on":[90],"lines":[92],"defined":[93],"by":[94,153],"fractions.":[97],"Temperature-dependence,":[98],"studies":[99],"demonstrated":[100],"that":[101],"differences":[102,119],"in":[103,115,120,129,149,156],"observed":[106],"different":[110],"densities":[112],"are":[113],"entropic":[114],"origin":[116],"reflect":[118],"ability":[122],"assume":[124],"special":[126],"configuration.":[127],"Differences":[128],"activation":[130],"entropy":[131],"can":[135],"be":[136],"empirically":[137],"correlated":[138],"activity.":[141,162],"latter":[143],"correlation":[144],"suggests":[145],"physical":[147],"similarity":[148],"roles":[151],"played":[152],"both":[157]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}