{"id":"https://openalex.org/W2034445959","doi":"https://doi.org/10.1371/journal.pone.0024392","title":"Cyclic AMP Control Measured in Two Compartments in HEK293 Cells: Phosphodiesterase KM Is More Important than Phosphodiesterase Localization","display_name":"Cyclic AMP Control Measured in Two Compartments in HEK293 Cells: Phosphodiesterase KM Is More Important than Phosphodiesterase Localization","publication_year":2011,"publication_date":"2011-09-08","ids":{"openalex":"https://openalex.org/W2034445959","doi":"https://doi.org/10.1371/journal.pone.0024392","mag":"2034445959","pmid":"https://pubmed.ncbi.nlm.nih.gov/21931705","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3169605"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0024392","doi":"https://doi.org/10.1371/journal.pone.0024392","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0024392","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0024392&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2034445959"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0024392&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061553329","display_name":"Karina Matthiesen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I133593841","display_name":"Lundbeck (Denmark)","ror":"https://ror.org/0564cd633","country_code":"DK","type":"company","lineage":["https://openalex.org/I133593841"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Karina Matthiesen","raw_affiliation_strings":["Synaptic Transmission 2, H. Lundbeck A/S, Copenhagen, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Synaptic Transmission 2, H. Lundbeck A/S, Copenhagen, Denmark","institution_ids":["https://openalex.org/I133593841"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088733516","display_name":"Jacob Nielsen","orcid":"https://orcid.org/0000-0001-9842-2276","observed_orcids":["https://orcid.org/0000-0001-9842-2276"]},"institutions":[{"id":"https://openalex.org/I133593841","display_name":"Lundbeck (Denmark)","ror":"https://ror.org/0564cd633","country_code":"DK","type":"company","lineage":["https://openalex.org/I133593841"]}],"countries":["DK"],"is_corresponding":true,"raw_author_name":"Jacob Nielsen","raw_affiliation_strings":["Synaptic Transmission 2, H. Lundbeck A/S, Copenhagen, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Synaptic Transmission 2, H. Lundbeck A/S, Copenhagen, Denmark","institution_ids":["https://openalex.org/I133593841"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5088733516"],"corresponding_institution_ids":["https://openalex.org/I133593841"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":1.4739,"has_fulltext":true,"cited_by_count":45,"citation_normalized_percentile":{"value":0.80144764,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"6","issue":"9","first_page":"e24392","last_page":"e24392"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12104","display_name":"Protein Tyrosine Phosphatases","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/camp","display_name":"cyclic AMP","score":0.828000009059906},{"id":"https://openalex.org/keywords/phosphodiesterase","display_name":"phosphodiesterase","score":0.8579999804496765},{"id":"https://openalex.org/keywords/compartmentalized-signaling","display_name":"compartmentalized signaling","score":0.8560000061988831},{"id":"https://openalex.org/keywords/bioluminescence-resonance-energy-transfer","display_name":"bioluminescence resonance energy transfer","score":0.6079999804496765},{"id":"https://openalex.org/keywords/phosphodiesterase-3","display_name":"phosphodiesterase 3","score":0.7699999809265137},{"id":"https://openalex.org/keywords/pde10a","display_name":"PDE10A","score":0.9480000138282776},{"id":"https://openalex.org/keywords/phosphodiesterase-4","display_name":"phosphodiesterase 4","score":1.0},{"id":"https://openalex.org/keywords/adenylate-cyclase","display_name":"adenylate cyclase","score":0.9319999814033508},{"id":"https://openalex.org/keywords/plasma-membrane","display_name":"plasma membrane","score":0.4620000123977661},{"id":"https://openalex.org/keywords/cytosol","display_name":"cytosol","score":0.9300000071525574},{"id":"https://openalex.org/keywords/hek293-cells","display_name":"HEK293 cells","score":0.8650000095367432}],"concepts":[{"id":"https://openalex.org/C98539663","wikidata":"https://www.wikidata.org/wiki/Q220599","display_name":"Cytosol","level":3,"score":0.8697470426559448},{"id":"https://openalex.org/C62826618","wikidata":"https://www.wikidata.org/wiki/Q424304","display_name":"Phosphodiesterase","level":3,"score":0.8269473314285278},{"id":"https://openalex.org/C2779178603","wikidata":"https://www.wikidata.org/wiki/Q356257","display_name":"Adenylyl cyclase","level":3,"score":0.8263584971427917},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.6598886251449585},{"id":"https://openalex.org/C58141971","wikidata":"https://www.wikidata.org/wiki/Q906688","display_name":"Phosphodiesterase 3","level":4,"score":0.6418924927711487},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.5626557469367981},{"id":"https://openalex.org/C163235415","wikidata":"https://www.wikidata.org/wiki/Q899814","display_name":"Second messenger system","level":3,"score":0.5579777359962463},{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.5503189563751221},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5150658488273621},{"id":"https://openalex.org/C79418042","wikidata":"https://www.wikidata.org/wiki/Q5864325","display_name":"PDE10A","level":4,"score":0.5092130899429321},{"id":"https://openalex.org/C2751546","wikidata":"https://www.wikidata.org/wiki/Q18042074","display_name":"ADCY10","level":5,"score":0.43290090560913086},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41275322437286377},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3928799033164978},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.357293963432312},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33860746026039124},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2755032181739807},{"id":"https://openalex.org/C69101867","wikidata":"https://www.wikidata.org/wiki/Q17709777","display_name":"ADCY9","level":4,"score":0.22408944368362427},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.1807997226715088},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.11099541187286377},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.0713367760181427},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000242","descriptor_name":"Cyclic AMP","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D000527","descriptor_name":"Alprostadil","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002451","descriptor_name":"Cell Compartmentation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D003600","descriptor_name":"Cytosol","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D003600","descriptor_name":"Cytosol","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D005576","descriptor_name":"Colforsin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010726","descriptor_name":"Phosphodiesterase Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010727","descriptor_name":"Phosphoric Diester Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D015374","descriptor_name":"Biosensing Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021381","descriptor_name":"Protein Transport","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D057809","descriptor_name":"HEK293 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060807","descriptor_name":"Bioluminescence Resonance Energy Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":7,"locations":[{"id":"doi:10.1371/journal.pone.0024392","doi":"https://doi.org/10.1371/journal.pone.0024392","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0024392","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0024392&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},{"id":"pmid:21931705","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21931705","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3169605","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC3169605/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.288.9466","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.288.9466","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/bb/6f/PLoS_One_2011_Sep_8_6(9)_e24392.tar.gz","raw_type":"text"},{"id":"pmh:oai:doaj.org/article:8e94489cc2b54aea91c6499fa304cfe1","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/8e94489cc2b54aea91c6499fa304cfe1","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 6, Iss 9, p e24392 (2011)","raw_type":"article"},{"id":"pmh:oai:figshare.com:article/133567","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/Cyclic_AMP_Control_Measured_in_Two_Compartments_in_HEK293_Cells_Phosphodiesterase_K_M_Is_More_Important_than_Phosphodiesterase_Localization/133567","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"},{"id":"pmh:oai:pure.atira.dk:publications/c2205619-11e0-4518-a31f-b9c9469dbf04","doi":null,"is_oa":false,"landing_page_url":"https://pure.au.dk/portal/en/publications/c2205619-11e0-4518-a31f-b9c9469dbf04","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Matthiesen, K & Nielsen, J 2011, 'Cyclic AMP Control Measured in Two Compartments in HEK293 Cells: Phosphodiesterase Km is More Important than Phosphodiesterase Localization', PLoS One, vol. 6, no. 9.","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0024392","doi":"https://doi.org/10.1371/journal.pone.0024392","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0024392","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0024392&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321992","display_name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Inova\u00e7\u00e3o","ror":"https://ror.org/050zdnc69"},{"id":"https://openalex.org/F4320327918","display_name":"H. Lundbeck A/S","ror":"https://ror.org/0564cd633"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2034445959.pdf","grobid_xml":"https://content.openalex.org/works/W2034445959.grobid-xml"},"referenced_works_count":34,"referenced_works":["https://openalex.org/W1511208409","https://openalex.org/W1541818284","https://openalex.org/W1543414519","https://openalex.org/W1550053345","https://openalex.org/W1954701421","https://openalex.org/W1963861627","https://openalex.org/W1965926793","https://openalex.org/W1967882515","https://openalex.org/W1967913552","https://openalex.org/W1969601547","https://openalex.org/W1971748110","https://openalex.org/W1975769625","https://openalex.org/W1991311961","https://openalex.org/W1994952789","https://openalex.org/W2015557754","https://openalex.org/W2058658179","https://openalex.org/W2061471552","https://openalex.org/W2073270529","https://openalex.org/W2074158980","https://openalex.org/W2078148536","https://openalex.org/W2081192590","https://openalex.org/W2086271620","https://openalex.org/W2087181092","https://openalex.org/W2088763860","https://openalex.org/W2089929045","https://openalex.org/W2092531574","https://openalex.org/W2103553832","https://openalex.org/W2107811381","https://openalex.org/W2113094198","https://openalex.org/W2133648715","https://openalex.org/W2143070083","https://openalex.org/W2143891666","https://openalex.org/W2166389885","https://openalex.org/W2403195379"],"related_works":["https://openalex.org/W1967913552","https://openalex.org/W129929294","https://openalex.org/W2122001651","https://openalex.org/W2137599759","https://openalex.org/W3174311122","https://openalex.org/W2038250633","https://openalex.org/W1968666638","https://openalex.org/W1995278218","https://openalex.org/W2053545620"],"abstract_inverted_index":{"The":[0,12],"intracellular":[1,68,217],"second":[2],"messenger":[3],"cyclic":[4],"AMP":[5],"(cAMP)":[6],"is":[7,21,37,107,222],"degraded":[8],"by":[9],"phosphodiesterases":[10],"(PDE).":[11],"knowledge":[13],"of":[14,19,55,154,177,198,227],"individual":[15],"families":[16],"and":[17,65,135,139,146,185,190,207],"subtypes":[18],"PDEs":[20,27,145,158,192,205],"considerable,":[22],"but":[23,168],"how":[24],"the":[25,30,56,76,99,104,142,149,161,175,178,182],"different":[26,196],"collaborate":[28],"in":[29,81,108,125,186],"cell":[31],"to":[32,44,73,94,103,164],"control":[33,226],"a":[34,52,89],"cAMP":[35,47,57,69,92,162,179,213,218,228],"signal":[36],"still":[38],"not":[39],"fully":[40],"understood.":[41],"In":[42],"order":[43],"investigate":[45],"compartmentalized":[46],"signaling,":[48],"we":[49,87,169,202],"have":[50,66,121,136],"generated":[51],"membrane-targeted":[53],"variant":[54],"Bioluminiscence":[58],"Resonance":[59,115],"Energy":[60,116],"Transfer":[61,117],"(BRET)":[62],"sensor":[63,79],"CAMYEL":[64,80],"compared":[67,102],"measurements":[70,74],"with":[71,75,110],"it":[72],"cytosolic":[77,151,157,191],"BRET":[78],"HEK293":[82,129],"cells.":[83],"With":[84],"these":[85],"sensors":[86],"observed":[88],"slightly":[90],"higher":[91,230],"response":[93,163,180],"adenylyl":[95,165],"cyclase":[96,166],"activation":[97],"at":[98,181,215,229],"plasma":[100,183],"membrane":[101,184],"cytosol,":[105],"which":[106],"accordance":[109],"earlier":[111],"results":[112],"from":[113,128],"Fluorescence":[114],"(FRET)":[118],"sensors.":[119],"We":[120],"analyzed":[122],"PDE":[123,133],"activity":[124],"fractionated":[126],"lysates":[127],"cells":[130],"using":[131],"selective":[132],"inhibitors":[134],"identified":[137],"PDE3":[138],"PDE10A":[140],"as":[141,148],"major":[143,150],"membrane-bound":[144,155,189],"PDE4":[147,221],"PDE.":[152],"Inhibition":[153],"or":[156],"can":[159],"potentiate":[160],"activation,":[167],"see":[170],"no":[171],"significant":[172],"difference":[173],"between":[174],"potentiation":[176],"cytosol":[187],"when":[188],"are":[193,209],"inhibited.":[194],"When":[195],"levels":[197],"stimulation":[199],"were":[200],"tested,":[201],"found":[203],"that":[204],"3":[206],"10":[208],"mainly":[210],"responsible":[211],"for":[212,225],"degradation":[214],"low":[216],"concentrations,":[219],"whereas":[220],"more":[223],"important":[224],"concentrations.":[231]},"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":6},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":8},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}