{"id":"https://openalex.org/W2034326100","doi":"https://doi.org/10.1080/00021369.1986.10867888","title":"Purification and Characterization of Rice Lipoxygenase Component 3 from Embryos","display_name":"Purification and Characterization of Rice Lipoxygenase Component 3 from Embryos","publication_year":1986,"publication_date":"1986-01-01","ids":{"openalex":"https://openalex.org/W2034326100","doi":"https://doi.org/10.1080/00021369.1986.10867888","mag":"2034326100"},"language":"en","primary_location":{"id":"doi:10.1080/00021369.1986.10867888","doi":"https://doi.org/10.1080/00021369.1986.10867888","is_oa":false,"landing_page_url":"https://doi.org/10.1080/00021369.1986.10867888","pdf_url":null,"source":{"id":"https://openalex.org/S2764346059","display_name":"Agricultural and Biological Chemistry","issn_l":"0002-1369","issn":["0002-1369","0375-8397","1881-1272","1881-1280"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Agricultural and Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jstage.jst.go.jp/article/bbb1961/50/12/50_12_3165/_pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5091691483","display_name":"Hiroyuki Ohta","orcid":"https://orcid.org/0000-0002-1068-3242","observed_orcids":["https://orcid.org/0000-0002-1068-3242","https://orcid.org/0000-0002-1816-7912"]},"institutions":[{"id":"https://openalex.org/I22299242","display_name":"Kyoto University","ror":"https://ror.org/02kpeqv85","country_code":"JP","type":"education","lineage":["https://openalex.org/I22299242"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroyuki Ohta","raw_affiliation_strings":["Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan","institution_ids":["https://openalex.org/I22299242"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035956714","display_name":"Shoji Ida","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I22299242","display_name":"Kyoto University","ror":"https://ror.org/02kpeqv85","country_code":"JP","type":"education","lineage":["https://openalex.org/I22299242"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shoji Ida","raw_affiliation_strings":["Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan","institution_ids":["https://openalex.org/I22299242"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088836243","display_name":"Bunzo Mikami","orcid":"https://orcid.org/0000-0003-0638-8619","observed_orcids":["https://orcid.org/0000-0003-0638-8619"]},"institutions":[{"id":"https://openalex.org/I22299242","display_name":"Kyoto University","ror":"https://ror.org/02kpeqv85","country_code":"JP","type":"education","lineage":["https://openalex.org/I22299242"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Bunzo Mikami","raw_affiliation_strings":["Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan","institution_ids":["https://openalex.org/I22299242"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109887188","display_name":"Yuhei Morita","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I22299242","display_name":"Kyoto University","ror":"https://ror.org/02kpeqv85","country_code":"JP","type":"education","lineage":["https://openalex.org/I22299242"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuhei Morita","raw_affiliation_strings":["Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan","institution_ids":["https://openalex.org/I22299242"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I22299242"],"apc_list":null,"apc_paid":null,"fwci":0.2038,"has_fulltext":false,"cited_by_count":43,"citation_normalized_percentile":{"value":0.35054122,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"50","issue":"12","first_page":"3165","last_page":"3171"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.3253999948501587,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.3253999948501587,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.27219998836517334,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.06279999762773514,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lipoxygenase-3","display_name":"LOX3","score":0.9150000214576721},{"id":"https://openalex.org/keywords/rice-embryo","display_name":"rice embryo","score":0.7110000252723694},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.9580000042915344},{"id":"https://openalex.org/keywords/non-heme-iron-proteins","display_name":"non-heme iron proteins","score":0.8619999885559082},{"id":"https://openalex.org/keywords/enzyme-inactivation","display_name":"enzyme inactivation","score":0.6520000100135803},{"id":"https://openalex.org/keywords/cysteine","display_name":"cysteine","score":0.6039999723434448},{"id":"https://openalex.org/keywords/glutathione","display_name":"glutathione","score":0.9959999918937683},{"id":"https://openalex.org/keywords/catalase","display_name":"catalase","score":0.9079999923706055},{"id":"https://openalex.org/keywords/enzyme-storage-stability","display_name":"enzyme storage stability","score":0.6949999928474426},{"id":"https://openalex.org/keywords/linoleic-acid","display_name":"linoleic acid","score":0.6240000128746033},{"id":"https://openalex.org/keywords/amino-acid-composition","display_name":"amino acid composition","score":0.6069999933242798},{"id":"https://openalex.org/keywords/soybean-lipoxygenase","display_name":"soybean lipoxygenase","score":0.7649999856948853},{"id":"https://openalex.org/keywords/rice-grain","display_name":"rice grain","score":0.40700000524520874}],"concepts":[{"id":"https://openalex.org/C161191863","wikidata":"https://www.wikidata.org/wiki/Q199655","display_name":"Library science","level":1,"score":0.6000040769577026},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4575151801109314},{"id":"https://openalex.org/C17744445","wikidata":"https://www.wikidata.org/wiki/Q36442","display_name":"Political science","level":0,"score":0.3587983250617981},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.1825057864189148}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.1080/00021369.1986.10867888","doi":"https://doi.org/10.1080/00021369.1986.10867888","is_oa":false,"landing_page_url":"https://doi.org/10.1080/00021369.1986.10867888","pdf_url":null,"source":{"id":"https://openalex.org/S2764346059","display_name":"Agricultural and Biological Chemistry","issn_l":"0002-1369","issn":["0002-1369","0375-8397","1881-1272","1881-1280"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Agricultural and Biological Chemistry","raw_type":"journal-article"},{"id":"doi:10.1271/bbb1961.50.3165","doi":"https://doi.org/10.1271/bbb1961.50.3165","is_oa":true,"landing_page_url":"https://doi.org/10.1271/bbb1961.50.3165","pdf_url":"https://www.jstage.jst.go.jp/article/bbb1961/50/12/50_12_3165/_pdf","source":{"id":"https://openalex.org/S2764346059","display_name":"Agricultural and Biological Chemistry","issn_l":"0002-1369","issn":["0002-1369","0375-8397","1881-1272","1881-1280"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Agricultural and Biological Chemistry","raw_type":"journal-article"},{"id":"pmh:oai:irdb.nii.ac.jp:00897:0003860596","doi":null,"is_oa":false,"landing_page_url":"http://t2r2.star.titech.ac.jp/cgi-bin/publicationinfo.cgi?q_publication_content_number=CTT100407425","pdf_url":null,"source":{"id":"https://openalex.org/S7407056385","display_name":"Institutional Repositories DataBase (IRDB)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I184597095","host_organization_name":"National Institute of Informatics","host_organization_lineage":["https://openalex.org/I184597095"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Agricultural and Biological Chemistry","raw_type":"journal article"},{"id":"pmh:oai:t2r2.star.titech.ac.jp:00012660","doi":null,"is_oa":false,"landing_page_url":"http://t2r2.star.titech.ac.jp/cgi-bin/publicationinfo.cgi?q_publication_content_number=CTT100407425","pdf_url":null,"source":{"id":"https://openalex.org/S4377196385","display_name":"Tokyo Tech Research Repository (Tokyo Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114531698","host_organization_name":"Tokyo Institute of Technology","host_organization_lineage":["https://openalex.org/I114531698"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1271/bbb1961.50.3165","doi":"https://doi.org/10.1271/bbb1961.50.3165","is_oa":true,"landing_page_url":"https://doi.org/10.1271/bbb1961.50.3165","pdf_url":"https://www.jstage.jst.go.jp/article/bbb1961/50/12/50_12_3165/_pdf","source":{"id":"https://openalex.org/S2764346059","display_name":"Agricultural and Biological Chemistry","issn_l":"0002-1369","issn":["0002-1369","0375-8397","1881-1272","1881-1280"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Agricultural and Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2034326100.pdf","grobid_xml":"https://content.openalex.org/works/W2034326100.grobid-xml"},"referenced_works_count":3,"referenced_works":["https://openalex.org/W1510642762","https://openalex.org/W1600496479","https://openalex.org/W6600077562"],"related_works":["https://openalex.org/W4242621489","https://openalex.org/W2102170354","https://openalex.org/W1560301115","https://openalex.org/W2410287884","https://openalex.org/W2159170527","https://openalex.org/W1990166134","https://openalex.org/W1978737180","https://openalex.org/W2015913439","https://openalex.org/W632627186","https://openalex.org/W2037322597"],"abstract_inverted_index":{"Lipoxygenase-3,":[0],"the":[1,5,49,56,67,71,93,111,150],"major":[2],"component":[3],"of":[4,16,26,51,55,70,152],"enzyme":[6,21,34,94,128,172],"in":[7,116,169,176],"rice":[8,177],"grain,":[9],"was":[10,35,46,137,173],"purified":[11,20,72,100,127],"2980-fold":[12],"with":[13],"a":[14,23,96,113,140],"yield":[15],"7%":[17],"from":[18],"embryos.The":[19],"had":[22],"specific":[24],"activity":[25],"280":[27],"^mol":[28],"O2":[29],"formed/min":[30],"per":[31,105],"mg":[32],"protein.This":[33],"inactivated":[36],"by":[37,48,58,77],"SH":[38],"compounds,":[39],"such":[40,160],"as":[41,139,161],"cysteine":[42],"and":[43,82,88,124,157,163,179,181,186],"glutathione.The":[44],"inactivation":[45],"prevented":[47],"addition":[50],"catalase":[52],"or":[53],"replacement":[54],"air":[57],"N2":[59],"gas.These":[60],"two":[61],"treatments":[62],"were":[63,86,119],"also":[64,174],"effective":[65],"for":[66],"stable":[68],"storage":[69],"enzyme.The":[73],"molecular":[74],"weights":[75],"measured":[76],"sodium":[78],"dodecyl":[79],"sulfate":[80],"gel":[81,84],"gradient":[83],"electrophoresis":[85],"93,000":[87],"89,000,":[89],"respectively,":[90],"indicating":[91],"that":[92,110],"is":[95,166],"single":[97],"polypeptide":[98],"chain.The":[99],"enzymecontained":[101],"0.73":[102],"Fe":[103],"atom":[104],"molecule.The":[106],"absorption":[107],"spectrum":[108],"suggested":[109],"enzymeis":[112],"non-hemeiron":[114],"protein.Somesimilarities":[115],"amino-acid":[117],"composition":[118],"observed":[120],"between":[121],"rice,":[122],"soybean,":[123],"pea":[125],"lipoxygenases.The":[126],"specifically":[129],"produced":[130],"9-dhydroperoxy-10,":[131],"12(is,Z)-octadecadienoic":[132],"acid":[133,136],"when":[134],"linoleic":[135,162],"used":[138],"substrate.Lipoxygenase":[141],"(LOX,":[142],"linoleate:":[143],"oxygen":[144],"oxidoreductase,":[145],"EC":[146],"1.13.1":[147],"1.12)":[148],"catalyzes":[149],"oxidation":[151],"methylene-interrupted":[153],"unsaturated":[154],"fatty":[155],"acids":[156],"their":[158],"esters":[159],"linolenic":[164],"acids.LOX":[165],"widely":[167],"distributed":[168],"higher":[170],"plants.1}The":[171],"found":[175],"bran2)":[178],"embryos,3)":[180],"three":[182],"components":[183],"(LOX-1,":[184],"LOX-2":[185],"LOX-3)":[187]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}