{"id":"https://openalex.org/W2033770631","doi":"https://doi.org/10.1128/aem.00499-07","title":"Changes in the Catalytic Properties of Pyrococcus furiosus Thermostable Amylase by Mutagenesis of the Substrate Binding Sites","display_name":"Changes in the Catalytic Properties of Pyrococcus furiosus Thermostable Amylase by Mutagenesis of the Substrate Binding Sites","publication_year":2007,"publication_date":"2007-07-14","ids":{"openalex":"https://openalex.org/W2033770631","doi":"https://doi.org/10.1128/aem.00499-07","mag":"2033770631","pmid":"https://pubmed.ncbi.nlm.nih.gov/17630303","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2042082"},"language":"en","primary_location":{"id":"doi:10.1128/aem.00499-07","doi":"https://doi.org/10.1128/aem.00499-07","is_oa":false,"landing_page_url":"https://doi.org/10.1128/aem.00499-07","pdf_url":null,"source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2042082/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101997963","display_name":"Sungjae Yang","orcid":"https://orcid.org/0000-0002-5513-5135","observed_orcids":["https://orcid.org/0000-0002-5513-5135"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Sung-Jae Yang","raw_affiliation_strings":["Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921","institution_ids":["https://openalex.org/I139264467"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Byoung-Chul Min","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Byoung-Chul Min","raw_affiliation_strings":["Starch and Sweetener Research and Development Center, BNS Division, Daesang Corporation, Ichon 467-813, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Starch and Sweetener Research and Development Center, BNS Division, Daesang Corporation, Ichon 467-813, Korea","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113950209","display_name":"Young\u2010Wan Kim","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Young-Wan Kim","raw_affiliation_strings":["Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921","institution_ids":["https://openalex.org/I139264467"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109283891","display_name":"Sangmok Jang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Sang-Mok Jang","raw_affiliation_strings":["Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921","institution_ids":["https://openalex.org/I139264467"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020256718","display_name":"Byong-Hoon Lee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Byong-Hoon Lee","raw_affiliation_strings":["Department of Food Science and Agricultural Chemistry, McGill University, Quebec H9X 3V9, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Science and Agricultural Chemistry, McGill University, Quebec H9X 3V9, Canada","institution_ids":["https://openalex.org/I5023651"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111844506","display_name":"Kwan\u2010Hwa Park","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Kwan-Hwa Park","raw_affiliation_strings":["Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921","institution_ids":["https://openalex.org/I139264467"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.1121,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.70830831,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"73","issue":"17","first_page":"5607","last_page":"5612"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8773999810218811,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8773999810218811,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.1193000003695488,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/substrate-binding-site","display_name":"substrate binding site","score":0.609000027179718},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.8510000109672546},{"id":"https://openalex.org/keywords/substrate-preference","display_name":"substrate preference","score":0.7559999823570251},{"id":"https://openalex.org/keywords/maltooligosaccharides","display_name":"maltooligosaccharides","score":0.9229999780654907},{"id":"https://openalex.org/keywords/beta-cyclodextrin","display_name":"\u03b2-cyclodextrin","score":0.8859999775886536},{"id":"https://openalex.org/keywords/glycoside-hydrolase-family-13","display_name":"glycoside hydrolase family 13","score":0.9229999780654907},{"id":"https://openalex.org/keywords/enzyme-catalytic-properties","display_name":"enzyme catalytic properties","score":0.7609999775886536},{"id":"https://openalex.org/keywords/enzyme-engineering","display_name":"enzyme engineering","score":0.3540000021457672}],"concepts":[{"id":"https://openalex.org/C2776943354","wikidata":"https://www.wikidata.org/wiki/Q146310","display_name":"Pyrococcus furiosus","level":4,"score":0.9982909560203552},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.6926714181900024},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.6309176683425903},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6122587323188782},{"id":"https://openalex.org/C170835558","wikidata":"https://www.wikidata.org/wiki/Q17153","display_name":"Amylase","level":3,"score":0.593643069267273},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5865223407745361},{"id":"https://openalex.org/C196032511","wikidata":"https://www.wikidata.org/wiki/Q375795","display_name":"Glycoside hydrolase","level":3,"score":0.563006579875946},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5254230499267578},{"id":"https://openalex.org/C9418097","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed evolution","level":4,"score":0.5198410153388977},{"id":"https://openalex.org/C142677202","wikidata":"https://www.wikidata.org/wiki/Q3062554","display_name":"Rubredoxin","level":2,"score":0.4551233947277069},{"id":"https://openalex.org/C2781216036","wikidata":"https://www.wikidata.org/wiki/Q902494","display_name":"Thermostability","level":3,"score":0.42991384863853455},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.42245927453041077},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3654715120792389},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.26382195949554443},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.25401079654693604},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13745960593223572},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.053993791341781616},{"id":"https://openalex.org/C550995028","wikidata":"https://www.wikidata.org/wiki/Q10872","display_name":"Archaea","level":3,"score":0.04744967818260193},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000516","descriptor_name":"alpha-Amylases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000516","descriptor_name":"alpha-Amylases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000516","descriptor_name":"alpha-Amylases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003505","descriptor_name":"Cyclodextrins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D007218","descriptor_name":"Industrial Microbiology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020027","descriptor_name":"Pyrococcus furiosus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D020027","descriptor_name":"Pyrococcus furiosus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1128/aem.00499-07","doi":"https://doi.org/10.1128/aem.00499-07","is_oa":false,"landing_page_url":"https://doi.org/10.1128/aem.00499-07","pdf_url":null,"source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},{"id":"pmid:17630303","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17630303","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and environmental microbiology","raw_type":"Evaluation Study"},{"id":"pmh:oai:pubmedcentral.nih.gov:2042082","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2042082/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2042082","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2042082/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W1580799101","https://openalex.org/W1828273185","https://openalex.org/W1963689341","https://openalex.org/W1969154123","https://openalex.org/W1978650586","https://openalex.org/W1979606794","https://openalex.org/W1984085600","https://openalex.org/W1991882432","https://openalex.org/W2004904454","https://openalex.org/W2006457326","https://openalex.org/W2009674410","https://openalex.org/W2015361638","https://openalex.org/W2019429599","https://openalex.org/W2026561040","https://openalex.org/W2035324090","https://openalex.org/W2041026677","https://openalex.org/W2053206272","https://openalex.org/W2058955066","https://openalex.org/W2064934481","https://openalex.org/W2071900732","https://openalex.org/W2074737756","https://openalex.org/W2085583312","https://openalex.org/W2110915660","https://openalex.org/W2110958200","https://openalex.org/W2113964973","https://openalex.org/W2117538889","https://openalex.org/W2122631536","https://openalex.org/W2124753857","https://openalex.org/W2128635872","https://openalex.org/W2133552794","https://openalex.org/W2143940130","https://openalex.org/W2145384337","https://openalex.org/W2160802133","https://openalex.org/W2320516304"],"related_works":["https://openalex.org/W1994472065","https://openalex.org/W2110915660","https://openalex.org/W2910217998","https://openalex.org/W2104729742","https://openalex.org/W2324708157","https://openalex.org/W2004153309","https://openalex.org/W4408556800","https://openalex.org/W2002560837","https://openalex.org/W611108046","https://openalex.org/W2003048469"],"abstract_inverted_index":{"Pyrococcus":[0],"furiosus":[1,38,52,114,140,143,149],"thermostable":[2],"amylase":[3],"(TA)":[4],"is":[5],"a":[6,11,70,93],"cyclodextrin":[7],"(CD)-degrading":[8],"enzyme":[9],"with":[10,86,145,151],"high":[12],"preference":[13,95,111],"for":[14,123,137],"CDs":[15,117],"over":[16,125],"maltooligosaccharides.":[17,119],"In":[18,81],"this":[19,176],"study,":[20],"we":[21],"investigated":[22],"the":[23,34,67,78,83,100,109],"roles":[24,169],"of":[25,36,50,69,72,99,112,132],"four":[26],"residues":[27],"(His414,":[28],"Gly415,":[29,163],"Met439,":[30,164],"and":[31,44,58,106,135,147,153,165,173],"Asp440)":[32],"in":[33,66,170,181],"function":[35],"P.":[37,51,113,139,142,148],"TA":[39,53,115,144,150],"by":[40,128],"using":[41],"site-directed":[42],"mutagenesis":[43],"kinetic":[45],"analysis.":[46],"A":[47],"variant":[48,84],"form":[49],"containing":[54],"two":[55],"mutations":[56,88,104],"(H414N":[57,89],"G415E)":[59,91],"exhibited":[60],"strongly":[61],"enhanced":[62],"alpha-(1,4)-transglycosylation":[63],"activity,":[64],"resulting":[65],"production":[68],"series":[71],"maltooligosaccharides":[73],"that":[74,98,161],"were":[75,156],"longer":[76],"than":[77],"initial":[79],"substrates.":[80],"contrast,":[82],"enzymes":[85],"single":[87],"or":[90],"showed":[92],"substrate":[94,110,121,171],"similar":[96],"to":[97,118],"wild-type":[101,138],"enzyme.":[102],"Other":[103],"(M439W":[105],"D440H)":[107],"reversed":[108],"from":[116],"Relative":[120],"preferences":[122],"maltoheptaose":[124],"beta-CD,":[126],"calculated":[127],"comparing":[129],"k(cat)/K(m)":[130],"ratios,":[131],"1,":[133],"8,":[134],"26":[136],"TA,":[141],"D440H,":[146,154],"M439W":[152],"respectively,":[155],"found.":[157],"Our":[158],"results":[159],"suggest":[160],"His414,":[162],"Asp440":[166],"play":[167],"important":[168],"recognition":[172],"transglycosylation.":[174],"Therefore,":[175],"study":[177],"provides":[178],"information":[179],"useful":[180],"engineering":[182],"glycoside":[183],"hydrolase":[184],"family":[185],"13":[186],"enzymes.":[187]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":4},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}