{"id":"https://openalex.org/W2033733508","doi":"https://doi.org/10.1074/jbc.m102799200","title":"The Death Effector Domain-associated Factor Plays Distinct Regulatory Roles in the Nucleus and Cytoplasm","display_name":"The Death Effector Domain-associated Factor Plays Distinct Regulatory Roles in the Nucleus and Cytoplasm","publication_year":2001,"publication_date":"2001-08-01","ids":{"openalex":"https://openalex.org/W2033733508","doi":"https://doi.org/10.1074/jbc.m102799200","mag":"2033733508","pmid":"https://pubmed.ncbi.nlm.nih.gov/11395500"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m102799200","doi":"https://doi.org/10.1074/jbc.m102799200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m102799200","pdf_url":"http://www.jbc.org/article/S0021925819315042/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925819315042/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088733725","display_name":"Lixin Zheng","orcid":"https://orcid.org/0000-0002-8838-8665","observed_orcids":["https://orcid.org/0000-0002-8838-8665"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lixin Zheng","raw_affiliation_strings":["Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA","Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"raw_affiliation_string":"Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 and","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074619588","display_name":"Olaf Schickling","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Olaf Schickling","raw_affiliation_strings":["The Ben May Institute for Cancer Research, University of Chicago, Chicago, Illinois 60637"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Ben May Institute for Cancer Research, University of Chicago, Chicago, Illinois 60637","institution_ids":["https://openalex.org/I40347166"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056606410","display_name":"Marcus E. Peter","orcid":"https://orcid.org/0000-0003-3216-036X","observed_orcids":["https://orcid.org/0000-0003-3216-036X"]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marcus E. Peter","raw_affiliation_strings":["The Ben May Institute for Cancer Research, University of Chicago, Chicago, Illinois 60637"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Ben May Institute for Cancer Research, University of Chicago, Chicago, Illinois 60637","institution_ids":["https://openalex.org/I40347166"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5071545468","display_name":"Michael J. Lenardo","orcid":"https://orcid.org/0000-0003-1584-468X","observed_orcids":["https://orcid.org/0000-0003-1584-468X"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Michael J. Lenardo","raw_affiliation_strings":["Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 and","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5071545468"],"corresponding_institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":1.8484,"has_fulltext":true,"cited_by_count":76,"citation_normalized_percentile":{"value":0.84657494,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"276","issue":"34","first_page":"31945","last_page":"31952"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10583","display_name":"Cancer-related Molecular Pathways","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11591","display_name":"NF-\u03baB Signaling Pathways","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/death-effector-domain","display_name":"death effector domain","score":0.8560000061988831},{"id":"https://openalex.org/keywords/death-receptor-mediated-apoptosis","display_name":"death receptor-mediated apoptosis","score":0.8999999761581421},{"id":"https://openalex.org/keywords/procaspase-8","display_name":"procaspase-8","score":0.8809999823570251},{"id":"https://openalex.org/keywords/fadd","display_name":"FADD","score":0.9700000286102295},{"id":"https://openalex.org/keywords/dedd","display_name":"DEDD","score":0.7160000205039978},{"id":"https://openalex.org/keywords/fas","display_name":"Fas","score":0.8479999899864197},{"id":"https://openalex.org/keywords/t-cell-activation","display_name":"T cell activation","score":0.7170000076293945},{"id":"https://openalex.org/keywords/nuclear-relocalization","display_name":"nuclear relocalization","score":0.656000018119812},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.628000020980835}],"concepts":[{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.8432420492172241},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.8109731674194336},{"id":"https://openalex.org/C2780723820","wikidata":"https://www.wikidata.org/wiki/Q1934178","display_name":"Nucleus","level":2,"score":0.7555565237998962},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6542844772338867},{"id":"https://openalex.org/C36503486","wikidata":"https://www.wikidata.org/wiki/Q11235244","display_name":"Domain (mathematical analysis)","level":2,"score":0.5016865730285645},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42364150285720825},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003593","descriptor_name":"Cytoplasm","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012097","descriptor_name":"Repressor Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020169","descriptor_name":"Caspases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D047908","descriptor_name":"Intracellular Signaling Peptides and Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053455","descriptor_name":"Caspase 10","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m102799200","doi":"https://doi.org/10.1074/jbc.m102799200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m102799200","pdf_url":"http://www.jbc.org/article/S0021925819315042/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:11395500","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11395500","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m102799200","doi":"https://doi.org/10.1074/jbc.m102799200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m102799200","pdf_url":"http://www.jbc.org/article/S0021925819315042/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2033733508.pdf","grobid_xml":"https://content.openalex.org/works/W2033733508.grobid-xml"},"referenced_works_count":24,"referenced_works":["https://openalex.org/W1577393853","https://openalex.org/W1836073275","https://openalex.org/W1911239883","https://openalex.org/W1980754795","https://openalex.org/W1985411011","https://openalex.org/W1986308149","https://openalex.org/W1988864413","https://openalex.org/W1992637685","https://openalex.org/W2000446104","https://openalex.org/W2024527294","https://openalex.org/W2025044046","https://openalex.org/W2029501678","https://openalex.org/W2032417513","https://openalex.org/W2036516702","https://openalex.org/W2072380594","https://openalex.org/W2081907462","https://openalex.org/W2091267060","https://openalex.org/W2094334993","https://openalex.org/W2101295809","https://openalex.org/W2105670328","https://openalex.org/W2108053853","https://openalex.org/W2114266096","https://openalex.org/W2157935840","https://openalex.org/W2158714788"],"related_works":["https://openalex.org/W1965667519","https://openalex.org/W1997789120","https://openalex.org/W1985081377","https://openalex.org/W2057694016","https://openalex.org/W2081907462","https://openalex.org/W1919860012","https://openalex.org/W2004789500","https://openalex.org/W2134238648","https://openalex.org/W1979118156","https://openalex.org/W1978133026"],"abstract_inverted_index":{"Homophilic":[0],"interactions":[1],"of":[2,13,25,57,143,187,193],"death":[3,14,148],"effector":[4],"domains":[5],"(DEDs)":[6],"are":[7],"crucial":[8],"for":[9],"the":[10,35,51,55,105,121,127,133,136,141,156,160,173,185],"signaling":[11],"pathways":[12],"receptor-mediated":[15],"apoptosis.":[16,154,194],"The":[17],"machinery":[18],"that":[19,67,179],"regulates":[20],"proper":[21],"oligomerization":[22],"and":[23,59,61,86,92,111,118,146,190],"autoactivation":[24],"procaspase-8":[26,58],"and/or":[27],"procaspase-10":[28,60,119],"during":[29],"T":[30],"lymphocyte":[31],"activation":[32],"determines":[33],"whether":[34],"cells":[36],"will":[37],"undergo":[38],"caspase-mediated":[39],"apoptosis":[40],"or":[41],"proliferation.":[42],"We":[43],"screened":[44],"a":[45,63,78,96,169],"yeast":[46],"two-hybrid":[47],"library":[48],"by":[49],"using":[50],"DEDs":[52],"contained":[53],"in":[54,108,120,132,172,184],"prodomains":[56],"isolated":[62],"DED-associated":[64],"factor":[65],"(DEDAF)":[66],"interacts":[68,114],"with":[69,115,126],"several":[70],"DED-containing":[71,128],"proteins":[72],"but":[73],"does":[74],"not":[75],"itself":[76],"contain":[77],"DED.":[79],"DEDAF":[80,101,113,139,158,180],"is":[81,93,102],"highly":[82],"conserved":[83],"between":[84],"human":[85],"mouse":[87],"(98%":[88],"amino":[89],"acid":[90],"identity)":[91],"homologous":[94],"to":[95,163,168],"nuclear":[97,191],"regulatory":[98],"protein":[99,130,162],"YAF-2.":[100],"expressed":[103],"at":[104],"highest":[106],"levels":[107],"lymphoid":[109],"tissues":[110],"placenta.":[112],"FADD,":[116],"procaspase-8,":[117],"cytosol":[122],"as":[123,125,150,152],"well":[124,151],"DNA-binding":[129],"(DEDD)":[131],"nucleus.":[134],"At":[135],"cell":[137],"membrane,":[138],"augmented":[140],"formation":[142],"CD95-FADD-caspase-8":[144],"complexes":[145],"enhanced":[147],"receptor-":[149],"DED-mediated":[153],"In":[155],"nucleus,":[157],"caused":[159],"DEDD":[161],"relocalize":[164],"from":[165],"subnuclear":[166],"structures":[167],"diffuse":[170],"distribution":[171],"nucleoplasm.":[174],"Our":[175],"data":[176],"therefore":[177],"suggest":[178],"may":[181],"be":[182],"involved":[183],"regulation":[186],"both":[188],"cytoplasmic":[189],"events":[192]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}