{"id":"https://openalex.org/W2033728613","doi":"https://doi.org/10.1104/pp.84.4.1185","title":"Factors Affecting the Level of Kanamycin Resistance in Transformed Sunflower Cells","display_name":"Factors Affecting the Level of Kanamycin Resistance in Transformed Sunflower Cells","publication_year":1987,"publication_date":"1987-08-01","ids":{"openalex":"https://openalex.org/W2033728613","doi":"https://doi.org/10.1104/pp.84.4.1185","mag":"2033728613","pmid":"https://pubmed.ncbi.nlm.nih.gov/16665582","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1056749"},"language":"en","primary_location":{"id":"doi:10.1104/pp.84.4.1185","doi":"https://doi.org/10.1104/pp.84.4.1185","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.84.4.1185","pdf_url":"https://academic.oup.com/plphys/article-pdf/84/4/1185/35637445/plphys_v84_4_1185.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/plphys/article-pdf/84/4/1185/35637445/plphys_v84_4_1185.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021923280","display_name":"Robert C. Nutter","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1336096307","display_name":"United States Department of Agriculture","ror":"https://ror.org/01na82s61","country_code":"US","type":"government","lineage":["https://openalex.org/I1336096307"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert Nutter","raw_affiliation_strings":["Department of Agricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Agricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":["https://openalex.org/I1336096307"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071972157","display_name":"Nicholas P. Everett","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nicholas Everett","raw_affiliation_strings":["Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087631349","display_name":"Dorothy A. Pierce","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dorothy Pierce","raw_affiliation_strings":["Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036486777","display_name":"Lucy Panganiban","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lucy Panganiban","raw_affiliation_strings":["Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006710753","display_name":"Patricia A. Okubara","orcid":"https://orcid.org/0000-0002-6838-5635","observed_orcids":["https://orcid.org/0000-0002-6838-5635"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Patricia Okubara","raw_affiliation_strings":["Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046223374","display_name":"Rosanna Lachmansingh","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Rosanna Lachmansingh","raw_affiliation_strings":["Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075918440","display_name":"Desmond D. Mascarenhas","orcid":"https://orcid.org/0000-0002-0710-9960","observed_orcids":["https://orcid.org/0000-0002-0710-9960"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Desmond Mascarenhas","raw_affiliation_strings":["Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Heather Welch","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Heather Welch","raw_affiliation_strings":["Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056268752","display_name":"Irvin J. Mettler","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Irvin Mettler","raw_affiliation_strings":["Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066905786","display_name":"Lucille Pomeroy","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lucille Pomeroy","raw_affiliation_strings":["Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006104220","display_name":"Jill L. Johnson","orcid":"https://orcid.org/0000-0003-3881-722X","observed_orcids":["https://orcid.org/0000-0003-3881-722X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jill Johnson","raw_affiliation_strings":["Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003338788","display_name":"John A. Howard","orcid":"https://orcid.org/0000-0002-5974-5416","observed_orcids":["https://orcid.org/0000-0002-5974-5416"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"John Howard","raw_affiliation_strings":["Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofAgricultural Biotechnology, Stauffer Chemical Company, Richmond, California 94804","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3851,"currency":"USD","value_usd":3851},"apc_paid":null,"fwci":0.4132,"has_fulltext":true,"cited_by_count":11,"citation_normalized_percentile":{"value":0.58036293,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"84","issue":"4","first_page":"1185","last_page":"1192"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/kanamycin-resistance","display_name":"kanamycin resistance","score":0.9800000190734863},{"id":"https://openalex.org/keywords/agrobacterium-tumefaciens","display_name":"Agrobacterium tumefaciens","score":0.8209999799728394},{"id":"https://openalex.org/keywords/promoter","display_name":"promoter","score":0.6159999966621399},{"id":"https://openalex.org/keywords/neomycin-phosphotransferase-ii","display_name":"neomycin phosphotransferase II","score":0.9670000076293945},{"id":"https://openalex.org/keywords/tn5","display_name":"Tn5","score":0.7929999828338623},{"id":"https://openalex.org/keywords/selectable-marker","display_name":"selectable marker","score":0.6069999933242798},{"id":"https://openalex.org/keywords/nopaline-synthase-promoter","display_name":"nopaline synthase promoter","score":0.7889999747276306},{"id":"https://openalex.org/keywords/genetically-modified-plants","display_name":"transgenic plants","score":0.46799999475479126}],"concepts":[{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.6933078765869141},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6896057724952698},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.671696662902832},{"id":"https://openalex.org/C2778741777","wikidata":"https://www.wikidata.org/wiki/Q2013941","display_name":"Octopine","level":5,"score":0.5952852964401245},{"id":"https://openalex.org/C2780920689","wikidata":"https://www.wikidata.org/wiki/Q27094615","display_name":"Kanamycin","level":3,"score":0.5749439597129822},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.531301736831665},{"id":"https://openalex.org/C2778972215","wikidata":"https://www.wikidata.org/wiki/Q8044002","display_name":"XhoI","level":5,"score":0.5162539482116699},{"id":"https://openalex.org/C42411736","wikidata":"https://www.wikidata.org/wiki/Q1550327","display_name":"Selectable marker","level":4,"score":0.5078669190406799},{"id":"https://openalex.org/C100012643","wikidata":"https://www.wikidata.org/wiki/Q1384844","display_name":"Expression cassette","level":5,"score":0.4937151372432709},{"id":"https://openalex.org/C98151556","wikidata":"https://www.wikidata.org/wiki/Q179545","display_name":"Ti plasmid","level":5,"score":0.4632960557937622},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4195757210254669},{"id":"https://openalex.org/C2776162087","wikidata":"https://www.wikidata.org/wiki/Q131472","display_name":"Agrobacterium tumefaciens","level":4,"score":0.2834136486053467},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.18728938698768616},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.10408169031143188},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.0990317165851593},{"id":"https://openalex.org/C130123407","wikidata":"https://www.wikidata.org/wiki/Q417754","display_name":"EcoRI","level":4,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1104/pp.84.4.1185","doi":"https://doi.org/10.1104/pp.84.4.1185","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.84.4.1185","pdf_url":"https://academic.oup.com/plphys/article-pdf/84/4/1185/35637445/plphys_v84_4_1185.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1056749","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1056749","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC1056749/pdf/plntphys00617-0231.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiol","raw_type":"Text"},{"id":"pmid:16665582","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16665582","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant physiology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1104/pp.84.4.1185","doi":"https://doi.org/10.1104/pp.84.4.1185","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.84.4.1185","pdf_url":"https://academic.oup.com/plphys/article-pdf/84/4/1185/35637445/plphys_v84_4_1185.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2033728613.pdf","grobid_xml":"https://content.openalex.org/works/W2033728613.grobid-xml"},"referenced_works_count":14,"referenced_works":["https://openalex.org/W56351758","https://openalex.org/W59983249","https://openalex.org/W137430743","https://openalex.org/W201648023","https://openalex.org/W796153325","https://openalex.org/W1551666311","https://openalex.org/W1988660721","https://openalex.org/W2005595259","https://openalex.org/W2012019347","https://openalex.org/W2024628569","https://openalex.org/W2079254989","https://openalex.org/W2085476855","https://openalex.org/W2408301476","https://openalex.org/W4298264454"],"related_works":["https://openalex.org/W2010568527","https://openalex.org/W2441267088","https://openalex.org/W2089650037","https://openalex.org/W2122098323","https://openalex.org/W2012152732","https://openalex.org/W2088392038","https://openalex.org/W2019301525","https://openalex.org/W2031696898","https://openalex.org/W1991872536","https://openalex.org/W41803340"],"abstract_inverted_index":{"A":[0],"230":[1],"base":[2,50],"pair":[3],"DNA":[4,53],"segment":[5],"containing":[6],"the":[7,11,36,56,60,67,73,83,117,121,126,135,142,147,154,165,176,182],"sequences":[8],"5'":[9,69,122],"to":[10,13,82,111,164],"700":[12],"750":[14],"nucleotide":[15],"(nt)":[16],"transcript":[17,155],"7'":[18],"(ORF":[19],"3;":[20],"RF":[21],"Barker,":[22],"KB":[23],"Idler,":[24],"DV":[25],"Thompson,":[26],"JD":[27],"Kemp":[28],"1983":[29],"Plant":[30],"Mol":[31],"Biol":[32],"2:":[33],"335-350)":[34],"of":[35,52,55,62,72,116,125,137,153,160],"octopine":[37,170],"tumor":[38,100,148],"inducing":[39,101],"plasmid":[40,102],"pTiA6":[41],"has":[42,47],"been":[43],"isolated.":[44],"This":[45],"region":[46,71,152],"(a)":[48],"180":[49],"pairs":[51],"upstream":[54],"TATA":[57],"box,":[58],"(b)":[59],"start":[61],"RNA":[63],"synthesis,":[64],"and":[65,141,169,179],"(c)":[66],"entire":[68],"untranslated":[70],"gene.":[74],"We":[75],"have":[76],"fused":[77],"this":[78,105],"presumed":[79],"promoter":[80,151,168,172],"fragment":[81],"neomycin":[84],"phosphotransferase":[85],"II":[86],"(NPTII)":[87],"gene":[88,128,157],"from":[89],"Tn5":[90],"in":[91,120,146,175,181],"a":[92,99],"plant":[93],"expression":[94],"cassette.":[95],"After":[96],"recombination":[97],"into":[98],"delivery":[103],"plasmid,":[104],"cassette":[106],"confers":[107],"selectable":[108],"kanamycin":[109,145,161],"resistance":[110,162],"transformed":[112],"sunflower":[113],"cells.":[114,149],"Removal":[115],"out-of-frame":[118],"ATG":[119],"leader":[123],"sequence":[124],"NPTII":[127,138],"by":[129],"two":[130],"different":[131],"modifications":[132],"increased":[133],"both":[134],"levels":[136,159],"enzyme":[139],"activity":[140],"ID(50)":[143],"for":[144],"The":[150],"7":[156],"gives":[158],"equivalent":[163],"nopaline":[166],"synthase":[167,171],"when":[173],"used":[174],"same":[177,183],"constructions":[178],"assayed":[180],"tissues.":[184]},"counts_by_year":[{"year":2020,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}