{"id":"https://openalex.org/W2032397728","doi":"https://doi.org/10.1107/s0907444902012787","title":"Crystal contacts engineering of aspartyl-tRNA synthetase from Thermus thermophilus: effects on crystallizability","display_name":"Crystal contacts engineering of aspartyl-tRNA synthetase from Thermus thermophilus: effects on crystallizability","publication_year":2002,"publication_date":"2002-09-26","ids":{"openalex":"https://openalex.org/W2032397728","doi":"https://doi.org/10.1107/s0907444902012787","mag":"2032397728","pmid":"https://pubmed.ncbi.nlm.nih.gov/12351895"},"language":"en","primary_location":{"id":"doi:10.1107/s0907444902012787","doi":"https://doi.org/10.1107/s0907444902012787","is_oa":false,"landing_page_url":"https://doi.org/10.1107/s0907444902012787","pdf_url":null,"source":{"id":"https://openalex.org/S114502960","display_name":"Acta Crystallographica Section D Biological Crystallography","issn_l":"0907-4449","issn":["0907-4449","1399-0047"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Crystallographica Section D Biological Crystallography","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063605653","display_name":"Christophe Charron","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210128885","display_name":"Architecture et R\u00e9activit\u00e9 de l'arN","ror":"https://ror.org/03xmjtz19","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210128885","https://openalex.org/I68947357"]},{"id":"https://openalex.org/I4210158086","display_name":"Institut de Biologie Mol\u00e9culaire et Cellulaire","ror":"https://ror.org/05qpmg879","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210158086","https://openalex.org/I68947357"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Christophe Charron","raw_affiliation_strings":["D\u00e9partement 'M\u00e9canismes et Macromol\u00e9cules de la Synth\u00e8se Prot\u00e9ique et Cristallogen\u00e8se', UPR 9002 ' 15 rue Ren\u00e9 Descartes, 67084 Strasbourg Cedex, France","Centre National de la Recherche Scientifique, Paris, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"D\u00e9partement 'M\u00e9canismes et Macromol\u00e9cules de la Synth\u00e8se Prot\u00e9ique et Cristallogen\u00e8se', UPR 9002 ' 15 rue Ren\u00e9 Descartes, 67084 Strasbourg Cedex, France","institution_ids":["https://openalex.org/I4210128885","https://openalex.org/I4210158086"]},{"raw_affiliation_string":"Centre National de la Recherche Scientifique, Paris, France","institution_ids":["https://openalex.org/I1294671590"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007226771","display_name":"Daniel Kern","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Daniel Kern","raw_affiliation_strings":["Centre National de la Recherche Scientifique, Paris, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre National de la Recherche Scientifique, Paris, France","institution_ids":["https://openalex.org/I1294671590"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5054694568","display_name":"Richard Gieg\u00e9","orcid":"https://orcid.org/0000-0001-9662-1482","observed_orcids":["https://orcid.org/0000-0001-9662-1482"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Richard Gieg\u00e9","raw_affiliation_strings":["Centre National de la Recherche Scientifique, Paris, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre National de la Recherche Scientifique, Paris, France","institution_ids":["https://openalex.org/I1294671590"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4740,"currency":"USD","value_usd":4740},"apc_paid":null,"fwci":0.7751,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.64231626,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"58","issue":"10","first_page":"1729","last_page":"1733"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11881","display_name":"Crystallization and Solubility Studies","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aspartyl-trna-synthetase","display_name":"aspartyl-tRNA synthetase","score":0.9850000143051147},{"id":"https://openalex.org/keywords/thermus-thermophilus","display_name":"Thermus thermophilus","score":0.996999979019165},{"id":"https://openalex.org/keywords/protein-crystallization","display_name":"protein crystallization","score":0.7099999785423279},{"id":"https://openalex.org/keywords/surface-residues","display_name":"surface residues","score":0.6129999756813049},{"id":"https://openalex.org/keywords/homodimer","display_name":"homodimer","score":0.7229999899864197},{"id":"https://openalex.org/keywords/crystalline-quality","display_name":"crystal quality","score":0.6140000224113464},{"id":"https://openalex.org/keywords/site-directed-mutants","display_name":"site-directed mutants","score":0.546999990940094}],"concepts":[{"id":"https://openalex.org/C2780883830","wikidata":"https://www.wikidata.org/wiki/Q139470","display_name":"Thermus thermophilus","level":4,"score":0.9354655742645264},{"id":"https://openalex.org/C37243968","wikidata":"https://www.wikidata.org/wiki/Q648961","display_name":"Orthorhombic crystal system","level":3,"score":0.8162145614624023},{"id":"https://openalex.org/C203036418","wikidata":"https://www.wikidata.org/wiki/Q284256","display_name":"Crystallization","level":2,"score":0.8080599308013916},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.6510912179946899},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6115437150001526},{"id":"https://openalex.org/C2781285689","wikidata":"https://www.wikidata.org/wiki/Q21921428","display_name":"Crystal (programming language)","level":2,"score":0.5152881145477295},{"id":"https://openalex.org/C153064111","wikidata":"https://www.wikidata.org/wiki/Q1281033","display_name":"Protein crystallization","level":3,"score":0.47709351778030396},{"id":"https://openalex.org/C115624301","wikidata":"https://www.wikidata.org/wiki/Q895901","display_name":"Crystal structure","level":2,"score":0.4769372344017029},{"id":"https://openalex.org/C153957851","wikidata":"https://www.wikidata.org/wiki/Q201448","display_name":"Transfer RNA","level":4,"score":0.4530794024467468},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.4365389943122864},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.4361231327056885},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.42306575179100037},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.34314996004104614},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.25359880924224854},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.1384943127632141},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.09591582417488098},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001226","descriptor_name":"Aspartate-tRNA Ligase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001226","descriptor_name":"Aspartate-tRNA Ligase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003460","descriptor_name":"Crystallization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006860","descriptor_name":"Hydrogen Bonding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013499","descriptor_name":"Surface Properties","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015394","descriptor_name":"Molecular Structure","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016963","descriptor_name":"Thermus thermophilus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D016963","descriptor_name":"Thermus thermophilus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046911","descriptor_name":"Macromolecular Substances","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1107/s0907444902012787","doi":"https://doi.org/10.1107/s0907444902012787","is_oa":false,"landing_page_url":"https://doi.org/10.1107/s0907444902012787","pdf_url":null,"source":{"id":"https://openalex.org/S114502960","display_name":"Acta Crystallographica Section D Biological Crystallography","issn_l":"0907-4449","issn":["0907-4449","1399-0047"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Crystallographica Section D Biological Crystallography","raw_type":"journal-article"},{"id":"pmid:12351895","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12351895","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta crystallographica. Section D, Biological crystallography","raw_type":"Journal Article"},{"id":"pmh:oai:HAL:hal-05254906v1","doi":null,"is_oa":false,"landing_page_url":"https://univoak.hal.science/hal-05254906","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"https://scripts.iucr.org/cgi-bin/paper?S0907444902012787","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1999226762","https://openalex.org/W1970299615","https://openalex.org/W2035281539","https://openalex.org/W2038567917","https://openalex.org/W2007682487","https://openalex.org/W1975303902","https://openalex.org/W1995862334","https://openalex.org/W2087085886","https://openalex.org/W1584043988","https://openalex.org/W2084792760"],"abstract_inverted_index":{"To":[0],"understand":[1],"how":[2],"surface":[3],"residues":[4],"in":[5,54,57],"a":[6,36,41,74],"protein":[7],"structure":[8],"influence":[9],"crystal":[10,15,17],"growth,":[11],"packing":[12,55,79],"arrangement":[13],"and":[14,21,81,115],"quality,":[16],"surfaces":[18],"were":[19],"modified":[20],"crystallizability":[22,82],"of":[23,43,50,62,66,70,78,83,89,98,104,111],"seven":[24],"different":[25],"mutants":[26,72],"investigated.":[27],"The":[28],"model":[29],"was":[30],"aspartyl-tRNA":[31],"synthetase-1":[32],"from":[33],"Thermus":[34],"thermophilus,":[35],"homodimer":[37],"(M(r)":[38],"122000)":[39],"with":[40],"subunit":[42],"580":[44],"amino":[45,51],"acids.":[46],"Engineering":[47],"concerned":[48],"modification":[49,88],"acids":[52],"involved":[53],"contacts":[56,91,106],"the":[58,63,67,71,84],"orthorhombic":[59],"lattice":[60,90],"(P2(1)2121)":[61],"synthetase.":[64],"Comparison":[65],"crystallization":[68,93],"behaviour":[69],"indicates":[73],"correlation":[75],"between":[76],"disruption/addition":[77],"interactions":[80],"mutants:":[85],"disruption":[86],"or":[87,94],"prevents":[92],"leads":[95,107],"to":[96,108],"crystals":[97,110],"poor":[99],"quality.":[100],"In":[101],"contrast,":[102],"addition":[103],"potential":[105],"well-shaped":[109],"same":[112],"space":[113],"group":[114],"cell":[116],"parameters":[117],"than":[118],"wild-type":[119],"crystals.":[120]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}