{"id":"https://openalex.org/W2032230786","doi":"https://doi.org/10.1074/jbc.275.14.9930","title":"Sequential Strand Exchange by XerC and XerD during Site-specific Recombination at dif","display_name":"Sequential Strand Exchange by XerC and XerD during Site-specific Recombination at dif","publication_year":2000,"publication_date":"2000-04-01","ids":{"openalex":"https://openalex.org/W2032230786","doi":"https://doi.org/10.1074/jbc.275.14.9930","mag":"2032230786","pmid":"https://pubmed.ncbi.nlm.nih.gov/10744667"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.275.14.9930","doi":"https://doi.org/10.1074/jbc.275.14.9930","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.275.14.9930","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.275.14.9930","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109237989","display_name":"Garry W. Blakely","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Garry W. Blakely","raw_affiliation_strings":["Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom","institution_ids":["https://openalex.org/I40120149"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111704306","display_name":"Anne O. Davidson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Anne O. Davidson","raw_affiliation_strings":["Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom","From the Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom","institution_ids":["https://openalex.org/I40120149"]},{"raw_affiliation_string":"From the Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom","institution_ids":["https://openalex.org/I40120149"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018039794","display_name":"David J. Sherratt","orcid":"https://orcid.org/0000-0002-2104-5430","observed_orcids":["https://orcid.org/0000-0002-2104-5430"]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"David J. Sherratt","raw_affiliation_strings":["Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom","From the Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom","institution_ids":["https://openalex.org/I40120149"]},{"raw_affiliation_string":"From the Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom","institution_ids":["https://openalex.org/I40120149"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5018039794"],"corresponding_institution_ids":["https://openalex.org/I40120149"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":1.2729,"has_fulltext":false,"cited_by_count":45,"citation_normalized_percentile":{"value":0.78170996,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"275","issue":"14","first_page":"9930","last_page":"9936"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.7567999958992004,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.7567999958992004,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.23649999499320984,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.00279999990016222,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/xerc","display_name":"XerC","score":0.9100000262260437},{"id":"https://openalex.org/keywords/xerd","display_name":"XerD","score":0.9980000257492065},{"id":"https://openalex.org/keywords/site-specific-recombination","display_name":"site-specific recombination","score":0.8149999976158142},{"id":"https://openalex.org/keywords/dif-site","display_name":"dif site","score":0.796999990940094},{"id":"https://openalex.org/keywords/tyrosine-recombinases","display_name":"tyrosine recombinases","score":0.8730000257492065},{"id":"https://openalex.org/keywords/holliday-junction","display_name":"Holliday junction","score":0.7760000228881836},{"id":"https://openalex.org/keywords/strand-exchange","display_name":"strand exchange","score":0.8360000252723694},{"id":"https://openalex.org/keywords/intermolecular-recombination","display_name":"intermolecular recombination","score":0.781000018119812},{"id":"https://openalex.org/keywords/intramolecular-recombination","display_name":"intramolecular recombination","score":0.902999997138977},{"id":"https://openalex.org/keywords/chromosome-segregation","display_name":"chromosome segregation","score":0.6959999799728394},{"id":"https://openalex.org/keywords/supercoiled-dna","display_name":"supercoiled DNA","score":0.7020000219345093}],"concepts":[{"id":"https://openalex.org/C102414288","wikidata":"https://www.wikidata.org/wiki/Q1172461","display_name":"Recombinase","level":4,"score":0.9445115327835083},{"id":"https://openalex.org/C130965112","wikidata":"https://www.wikidata.org/wiki/Q1624837","display_name":"Holliday junction","level":4,"score":0.8869360685348511},{"id":"https://openalex.org/C119898374","wikidata":"https://www.wikidata.org/wiki/Q7531737","display_name":"Site-specific recombination","level":5,"score":0.835503339767456},{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.6327832937240601},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5987803339958191},{"id":"https://openalex.org/C156695909","wikidata":"https://www.wikidata.org/wiki/Q3373825","display_name":"Recombination","level":3,"score":0.5370733737945557},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.480745792388916},{"id":"https://openalex.org/C185581394","wikidata":"https://www.wikidata.org/wiki/Q145911","display_name":"Base pair","level":3,"score":0.47443142533302307},{"id":"https://openalex.org/C201437064","wikidata":"https://www.wikidata.org/wiki/Q3175967","display_name":"Synapsis","level":4,"score":0.4601519703865051},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4244413375854492},{"id":"https://openalex.org/C2909909245","wikidata":"https://www.wikidata.org/wiki/Q412550","display_name":"Integrases","level":4,"score":0.42381733655929565},{"id":"https://openalex.org/C18103114","wikidata":"https://www.wikidata.org/wiki/Q1720369","display_name":"Replicon","level":4,"score":0.42248231172561646},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4198302924633026},{"id":"https://openalex.org/C135300049","wikidata":"https://www.wikidata.org/wiki/Q5426559","display_name":"FLP-FRT recombination","level":5,"score":0.4117946922779083},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.38059213757514954},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.28672266006469727},{"id":"https://openalex.org/C101434241","wikidata":"https://www.wikidata.org/wiki/Q211675","display_name":"Genetic recombination","level":4,"score":0.2770831286907196},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0631033182144165}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002876","descriptor_name":"Chromosomes, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004254","descriptor_name":"DNA Nucleotidyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004254","descriptor_name":"DNA Nucleotidyltransferases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004278","descriptor_name":"DNA, Superhelical","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009838","descriptor_name":"Oligodeoxyribonucleotides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012093","descriptor_name":"Replicon","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019281","descriptor_name":"Dimerization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D045522","descriptor_name":"Recombinases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.275.14.9930","doi":"https://doi.org/10.1074/jbc.275.14.9930","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.275.14.9930","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:10744667","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10744667","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.275.14.9930","doi":"https://doi.org/10.1074/jbc.275.14.9930","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.275.14.9930","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W134540825","https://openalex.org/W159023385","https://openalex.org/W1204884779","https://openalex.org/W1532522639","https://openalex.org/W1538904365","https://openalex.org/W1590546489","https://openalex.org/W1967071624","https://openalex.org/W1973964227","https://openalex.org/W1979784554","https://openalex.org/W1987456338","https://openalex.org/W2006444555","https://openalex.org/W2009355359","https://openalex.org/W2016988643","https://openalex.org/W2019635509","https://openalex.org/W2023054266","https://openalex.org/W2029322879","https://openalex.org/W2030864530","https://openalex.org/W2031812389","https://openalex.org/W2033270508","https://openalex.org/W2039509485","https://openalex.org/W2044728326","https://openalex.org/W2057920617","https://openalex.org/W2065859262","https://openalex.org/W2066572518","https://openalex.org/W2083301155","https://openalex.org/W2093125979","https://openalex.org/W2094626148","https://openalex.org/W2118854117","https://openalex.org/W2119022116","https://openalex.org/W2123406564","https://openalex.org/W2124408385","https://openalex.org/W2150039400","https://openalex.org/W2170094750","https://openalex.org/W2171184423","https://openalex.org/W2403119818"],"related_works":["https://openalex.org/W2122195928","https://openalex.org/W2053286804","https://openalex.org/W1538904365","https://openalex.org/W141407422","https://openalex.org/W1873207578","https://openalex.org/W2041645335","https://openalex.org/W134540825","https://openalex.org/W759107234","https://openalex.org/W2088759721","https://openalex.org/W2596539719"],"abstract_inverted_index":{"Successful":[0],"segregation":[1],"of":[2,40,148,167],"circular":[3,41],"chromosomes":[4],"in":[5,73,81,153],"Escherichia":[6],"coli":[7],"requires":[8],"that":[9,77,128],"dimeric":[10],"replicons,":[11],"produced":[12],"by":[13,97,110,118,138,151],"homologous":[14],"recombination,":[15],"are":[16],"converted":[17,113],"to":[18,21,37,65,114],"monomers":[19],"prior":[20],"cell":[22],"division.":[23],"The":[24],"Xer":[25],"site-specific":[26,71],"recombination":[27,72,80],"system":[28],"uses":[29],"two":[30],"related":[31],"tyrosine":[32],"recombinases,":[33],"XerC":[34,111,130],"and":[35,69,89,145,159],"XerD,":[36],"catalyze":[38],"resolution":[39],"dimers":[42],"at":[43],"the":[44,54,63,101,104,164],"chromosomal":[45],"site,":[46],"dif.":[47],"A":[48],"33-base":[49],"pair":[50,56],"DNA":[51,92],"fragment":[52],"containing":[53,93],"28-base":[55],"minimal":[57],"dif":[58,86,94],"site":[59],"is":[60,95,112],"sufficient":[61],"for":[62],"recombinases":[64],"mediate":[66],"both":[67],"inter-":[68],"intramolecular":[70],"vivo.":[74],"We":[75,125],"show":[76],"Xer-mediated":[78],"intermolecular":[79,155],"vitro":[82],"between":[83,157],"nicked":[84,105,142],"linear":[85,116,143,158],"\"suicide\"":[87],"substrates":[88],"supercoiled":[90,160],"plasmid":[91],"initiated":[96],"XerC.":[98],"Furthermore,":[99],"on":[100,140],"appropriate":[102],"substrate,":[103],"Holliday":[106],"junction":[107],"intermediate":[108],"formed":[109],"a":[115,119,129,141],"product":[117],"subsequent":[120],"single":[121],"XerD-mediated":[122],"strand":[123,136,149,168],"exchange.":[124],"also":[126],"demonstrate":[127],"homologue":[131],"from":[132],"Pseudomonas":[133],"aeruginosa":[134],"stimulates":[135],"cleavage":[137],"XerD":[139,152],"substrate":[144],"promotes":[146],"initiation":[147],"exchange":[150],"an":[154],"reaction":[156],"DNA,":[161],"thereby":[162],"reversing":[163],"normal":[165],"order":[166],"exchanges.":[169]},"counts_by_year":[{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}