{"id":"https://openalex.org/W2031782001","doi":"https://doi.org/10.1186/s12934-015-0246-0","title":"Iterative carotenogenic screens identify combinations of yeast gene deletions that enhance sclareol production","display_name":"Iterative carotenogenic screens identify combinations of yeast gene deletions that enhance sclareol production","publication_year":2015,"publication_date":"2015-04-23","ids":{"openalex":"https://openalex.org/W2031782001","doi":"https://doi.org/10.1186/s12934-015-0246-0","mag":"2031782001","pmid":"https://pubmed.ncbi.nlm.nih.gov/25903744","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4413541"},"language":"en","primary_location":{"id":"doi:10.1186/s12934-015-0246-0","doi":"https://doi.org/10.1186/s12934-015-0246-0","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12934-015-0246-0","pdf_url":"https://microbialcellfactories.biomedcentral.com/counter/pdf/10.1186/s12934-015-0246-0","source":{"id":"https://openalex.org/S171368329","display_name":"Microbial Cell Factories","issn_l":"1475-2859","issn":["1475-2859"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbial Cell Factories","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://microbialcellfactories.biomedcentral.com/counter/pdf/10.1186/s12934-015-0246-0","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111930651","display_name":"Fotini A. Trikka","orcid":"https://orcid.org/0000-0003-2445-2039","observed_orcids":["https://orcid.org/0000-0003-2445-2039"]},"institutions":[{"id":"https://openalex.org/I4210134249","display_name":"Centre for Research and Technology Hellas","ror":"https://ror.org/03bndpq63","country_code":"GR","type":"facility","lineage":["https://openalex.org/I4210134249"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"Fotini A Trikka","raw_affiliation_strings":["Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. fotaaki@hotmail.co.uk"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. fotaaki@hotmail.co.uk","institution_ids":["https://openalex.org/I4210134249"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020980294","display_name":"Alexandros K. Nikolaidis","orcid":"https://orcid.org/0000-0002-9191-0573","observed_orcids":["https://orcid.org/0000-0002-9191-0573"]},"institutions":[{"id":"https://openalex.org/I4210134249","display_name":"Centre for Research and Technology Hellas","ror":"https://ror.org/03bndpq63","country_code":"GR","type":"facility","lineage":["https://openalex.org/I4210134249"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"Alexandros Nikolaidis","raw_affiliation_strings":["Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. nikolchem@hotmail.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. nikolchem@hotmail.com","institution_ids":["https://openalex.org/I4210134249"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015809332","display_name":"Anastasia Athanasakoglou","orcid":"https://orcid.org/0000-0003-2607-6292","observed_orcids":["https://orcid.org/0000-0003-2607-6292"]},"institutions":[{"id":"https://openalex.org/I142617266","display_name":"University of Crete","ror":"https://ror.org/00dr28g20","country_code":"GR","type":"education","lineage":["https://openalex.org/I142617266"]},{"id":"https://openalex.org/I4210134249","display_name":"Centre for Research and Technology Hellas","ror":"https://ror.org/03bndpq63","country_code":"GR","type":"facility","lineage":["https://openalex.org/I4210134249"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"Anastasia Athanasakoglou","raw_affiliation_strings":["Department of Biochemistry, School of Medicine, University of Crete, P.O. Box 2208, Heraklion, 71003, Greece. ath.anastasia@gmail.com","Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. ath.anastasia@gmail.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, School of Medicine, University of Crete, P.O. Box 2208, Heraklion, 71003, Greece. ath.anastasia@gmail.com","institution_ids":["https://openalex.org/I142617266"]},{"raw_affiliation_string":"Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. ath.anastasia@gmail.com","institution_ids":["https://openalex.org/I4210134249"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077745431","display_name":"Aggeliki Andreadelli","orcid":"https://orcid.org/0000-0002-5559-6812","observed_orcids":["https://orcid.org/0000-0002-5559-6812"]},"institutions":[{"id":"https://openalex.org/I4210134249","display_name":"Centre for Research and Technology Hellas","ror":"https://ror.org/03bndpq63","country_code":"GR","type":"facility","lineage":["https://openalex.org/I4210134249"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"Aggeliki Andreadelli","raw_affiliation_strings":["Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. a.andreadelli@gmail.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. a.andreadelli@gmail.com","institution_ids":["https://openalex.org/I4210134249"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035239204","display_name":"Codru\u0163a Ignea","orcid":"https://orcid.org/0000-0002-6304-9413","observed_orcids":["https://orcid.org/0000-0002-6304-9413"]},"institutions":[{"id":"https://openalex.org/I142617266","display_name":"University of Crete","ror":"https://ror.org/00dr28g20","country_code":"GR","type":"education","lineage":["https://openalex.org/I142617266"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"Codruta Ignea","raw_affiliation_strings":["Department of Biochemistry, School of Medicine, University of Crete, P.O. Box 2208, Heraklion, 71003, Greece. igneacodruta@yahoo.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, School of Medicine, University of Crete, P.O. Box 2208, Heraklion, 71003, Greece. igneacodruta@yahoo.com","institution_ids":["https://openalex.org/I142617266"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038566355","display_name":"Konstantia Kotta","orcid":"https://orcid.org/0000-0003-1278-8897","observed_orcids":["https://orcid.org/0000-0003-1278-8897"]},"institutions":[{"id":"https://openalex.org/I4210134249","display_name":"Centre for Research and Technology Hellas","ror":"https://ror.org/03bndpq63","country_code":"GR","type":"facility","lineage":["https://openalex.org/I4210134249"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"Konstantia Kotta","raw_affiliation_strings":["Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. ntina_kotta@yahoo.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. ntina_kotta@yahoo.com","institution_ids":["https://openalex.org/I4210134249"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059862506","display_name":"Anagnostis Argiriou","orcid":"https://orcid.org/0000-0002-9164-632X","observed_orcids":["https://orcid.org/0000-0002-9164-632X"]},"institutions":[{"id":"https://openalex.org/I4210134249","display_name":"Centre for Research and Technology Hellas","ror":"https://ror.org/03bndpq63","country_code":"GR","type":"facility","lineage":["https://openalex.org/I4210134249"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"Anagnostis Argiriou","raw_affiliation_strings":["Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. argiriou@certh.gr"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. argiriou@certh.gr","institution_ids":["https://openalex.org/I4210134249"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074248081","display_name":"Sotirios C. Kampranis","orcid":"https://orcid.org/0000-0001-6208-1684","observed_orcids":["https://orcid.org/0000-0001-6208-1684"]},"institutions":[{"id":"https://openalex.org/I142617266","display_name":"University of Crete","ror":"https://ror.org/00dr28g20","country_code":"GR","type":"education","lineage":["https://openalex.org/I142617266"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"Sotirios C Kampranis","raw_affiliation_strings":["Department of Biochemistry, School of Medicine, University of Crete, P.O. Box 2208, Heraklion, 71003, Greece. s.kampranis@med.uoc.gr"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, School of Medicine, University of Crete, P.O. Box 2208, Heraklion, 71003, Greece. s.kampranis@med.uoc.gr","institution_ids":["https://openalex.org/I142617266"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5040048441","display_name":"Antonios M. Makris","orcid":"https://orcid.org/0000-0002-0661-7795","observed_orcids":["https://orcid.org/0000-0002-0661-7795"]},"institutions":[{"id":"https://openalex.org/I4210134249","display_name":"Centre for Research and Technology Hellas","ror":"https://ror.org/03bndpq63","country_code":"GR","type":"facility","lineage":["https://openalex.org/I4210134249"]}],"countries":["GR"],"is_corresponding":true,"raw_author_name":"Antonios M Makris","raw_affiliation_strings":["Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. antoniosmakris@yahoo.gr"],"raw_orcid":"https://orcid.org/0000-0002-0661-7795","affiliations":[{"raw_affiliation_string":"Institute of Applied Biosciences/CERTH, P.O. Box 60361, Thermi, 57001, Thessaloniki, Greece. antoniosmakris@yahoo.gr","institution_ids":["https://openalex.org/I4210134249"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5040048441"],"corresponding_institution_ids":["https://openalex.org/I4210134249"],"apc_list":{"value":3590,"currency":"USD","value_usd":3590},"apc_paid":{"value":3590,"currency":"USD","value_usd":3590},"fwci":1.694,"has_fulltext":true,"cited_by_count":75,"citation_normalized_percentile":{"value":0.83793848,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"14","issue":"1","first_page":"60","last_page":"60"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.7265999913215637,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.7265999913215637,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.26170000433921814,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.003599999938160181,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sclareol","display_name":"sclareol","score":0.9739999771118164},{"id":"https://openalex.org/keywords/carotenoid-production","display_name":"carotenoid production","score":0.7889999747276306},{"id":"https://openalex.org/keywords/iterative-selection","display_name":"iterative selection","score":0.5370000004768372},{"id":"https://openalex.org/keywords/diterpenes","display_name":"diterpenoids","score":0.7699999809265137},{"id":"https://openalex.org/keywords/sterol-biosynthesis","display_name":"sterol biosynthesis","score":0.7170000076293945},{"id":"https://openalex.org/keywords/yarrowia-lipolytica","display_name":"Yarrowia lipolytica","score":0.6460000276565552},{"id":"https://openalex.org/keywords/terpenoid-biosynthesis","display_name":"terpenoid biosynthesis","score":0.6230000257492065},{"id":"https://openalex.org/keywords/genetic-interactions","display_name":"genetic interactions","score":0.42399999499320984}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7541614770889282},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6197733879089355},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6162752509117126},{"id":"https://openalex.org/C2777910421","wikidata":"https://www.wikidata.org/wiki/Q143263","display_name":"Ergosterol","level":2,"score":0.5924229621887207},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.5823407173156738},{"id":"https://openalex.org/C2778022156","wikidata":"https://www.wikidata.org/wiki/Q576145","display_name":"Strain (injury)","level":2,"score":0.53904789686203},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.5195453763008118},{"id":"https://openalex.org/C206103511","wikidata":"https://www.wikidata.org/wiki/Q426694","display_name":"Terpenoid","level":2,"score":0.5001564025878906},{"id":"https://openalex.org/C28781525","wikidata":"https://www.wikidata.org/wiki/Q191907","display_name":"Carotenoid","level":2,"score":0.4377847909927368},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.41381603479385376},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3946838974952698},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.36006200313568115},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3298056125640869},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002338","descriptor_name":"Carotenoids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004224","descriptor_name":"Diterpenes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004875","descriptor_name":"Ergosterol","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D004875","descriptor_name":"Ergosterol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013729","descriptor_name":"Terpenes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1186/s12934-015-0246-0","doi":"https://doi.org/10.1186/s12934-015-0246-0","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12934-015-0246-0","pdf_url":"https://microbialcellfactories.biomedcentral.com/counter/pdf/10.1186/s12934-015-0246-0","source":{"id":"https://openalex.org/S171368329","display_name":"Microbial Cell Factories","issn_l":"1475-2859","issn":["1475-2859"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbial Cell Factories","raw_type":"journal-article"},{"id":"pmid:25903744","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25903744","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbial cell factories","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4413541","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC4413541/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microb Cell Fact","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1186/s12934-015-0246-0","doi":"https://doi.org/10.1186/s12934-015-0246-0","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12934-015-0246-0","pdf_url":"https://microbialcellfactories.biomedcentral.com/counter/pdf/10.1186/s12934-015-0246-0","source":{"id":"https://openalex.org/S171368329","display_name":"Microbial Cell Factories","issn_l":"1475-2859","issn":["1475-2859"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbial Cell Factories","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Responsible consumption and production","id":"https://metadata.un.org/sdg/12","score":0.6000000238418579}],"study_designs":[],"awards":[{"id":"https://openalex.org/G6552484555","display_name":null,"funder_award_id":"09\u03a3\u03a5\u039d-23-879","funder_id":"https://openalex.org/F4320321917","funder_display_name":"General Secretariat for Research and Technology"}],"funders":[{"id":"https://openalex.org/F4320321917","display_name":"General Secretariat for Research and Technology","ror":"https://ror.org/04yeh8h63"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2031782001.pdf","grobid_xml":"https://content.openalex.org/works/W2031782001.grobid-xml"},"referenced_works_count":48,"referenced_works":["https://openalex.org/W69390791","https://openalex.org/W204275195","https://openalex.org/W276882634","https://openalex.org/W581670567","https://openalex.org/W1499193052","https://openalex.org/W1566952502","https://openalex.org/W1599134891","https://openalex.org/W1937587271","https://openalex.org/W1952627112","https://openalex.org/W1978806418","https://openalex.org/W1979964161","https://openalex.org/W1988420806","https://openalex.org/W1989060614","https://openalex.org/W1990226486","https://openalex.org/W1999723404","https://openalex.org/W1999771206","https://openalex.org/W2013578400","https://openalex.org/W2017353229","https://openalex.org/W2062042850","https://openalex.org/W2064627233","https://openalex.org/W2072369409","https://openalex.org/W2082995554","https://openalex.org/W2095999574","https://openalex.org/W2109370496","https://openalex.org/W2112168018","https://openalex.org/W2112906241","https://openalex.org/W2124838044","https://openalex.org/W2128897939","https://openalex.org/W2129288998","https://openalex.org/W2130373334","https://openalex.org/W2131607454","https://openalex.org/W2132444568","https://openalex.org/W2133102806","https://openalex.org/W2134039693","https://openalex.org/W2138572689","https://openalex.org/W2138885877","https://openalex.org/W2139850148","https://openalex.org/W2147257317","https://openalex.org/W2147366327","https://openalex.org/W2147545320","https://openalex.org/W2149202606","https://openalex.org/W2158327382","https://openalex.org/W2160913151","https://openalex.org/W2165630230","https://openalex.org/W2326149834","https://openalex.org/W2332641124","https://openalex.org/W2726651211","https://openalex.org/W4240908898"],"related_works":["https://openalex.org/W1978806418","https://openalex.org/W2413403137","https://openalex.org/W1964914469","https://openalex.org/W2124838044","https://openalex.org/W2121037503","https://openalex.org/W1979964161","https://openalex.org/W2923912157","https://openalex.org/W2129288998","https://openalex.org/W2309434533","https://openalex.org/W1967084983"],"abstract_inverted_index":{"BACKGROUND:":[0],"Terpenoids":[1],"(isoprenoids)":[2],"have":[3,35,254],"numerous":[4],"applications":[5],"in":[6,33,163,174,207,212,219,259],"flavors,":[7],"fragrances,":[8],"drugs":[9],"and":[10,91,135,152,168,200,202,209,239,247],"biofuels.":[11],"The":[12,160],"number":[13,149],"of":[14,39,42,55,71,75,106,122,150,178,186,232,250,276],"microbially":[15],"produced":[16],"terpenoids":[17],"is":[18],"increasing":[19],"as":[20],"new":[21,176],"biosynthetic":[22,45],"pathways":[23],"are":[24],"being":[25],"elucidated.":[26],"However,":[27],"efforts":[28],"to":[29,61,84,112,126,147,183],"improve":[30,237],"terpenoid":[31],"production":[32,241],"yeast":[34,64,233],"mostly":[36],"taken":[37],"advantage":[38],"existing":[40],"knowledge":[41],"the":[43,53,56,79,100,138,148,169,184,251,264],"sterol":[44,260],"pathway,":[46],"while":[47],"many":[48],"additional":[49],"factors":[50],"may":[51],"affect":[52],"output":[54],"engineered":[57],"system.":[58],"RESULTS:":[59],"Aiming":[60],"develop":[62],"a":[63,73,97,114,175,204,268],"strain":[65,171,191,245],"that":[66,88,124,236],"can":[67],"support":[68],"high":[69],"titers":[70,242],"sclareol,":[72,94],"diterpene":[74],"great":[76],"importance":[77],"for":[78,116,156],"perfume":[80],"industry,":[81],"we":[82,228],"sought":[83],"identify":[85,120],"gene":[86,141,161,234],"deletions":[87,123,142,194,235,253,277],"improved":[89,281],"carotenoid,":[90],"thus":[92],"potentially":[93],"production.":[95],"Using":[96,224],"carotenogenic":[98,131],"screen,":[99],"best":[101],"100":[102],"deletion":[103],"mutants,":[104],"out":[105],"4,700":[107],"mutant":[108],"strains,":[109],"were":[110,133,143],"selected":[111,162],"create":[113],"subset":[115],"further":[117,127,144],"analysis.":[118],"To":[119],"combinations":[121,231],"cooperate":[125],"boost":[128],"production,":[129],"iterative":[130,226],"screens":[132],"applied,":[134],"each":[136,157,164],"time":[137],"top":[139],"performing":[140],"ranked":[145],"according":[146],"genetic":[151],"physical":[153],"interactions":[154],"known":[155],"specific":[158],"gene.":[159],"round":[165,177],"was":[166,172],"deleted":[167],"resulting":[170],"employed":[173],"selection.":[179],"This":[180],"approach":[181,266],"led":[182],"development":[185],"an":[187,225],"EG60":[188],"derived":[189],"haploid":[190],"combining":[192],"six":[193],"(rox1,":[195],"dos2,":[196],"yer134c,":[197],"vba5,":[198],"ynr063w":[199],"ygr259c)":[201],"exhibiting":[203],"40-fold":[205],"increase":[206,211],"carotenoid":[208,238],"12-fold":[210],"sclareol":[213,218,240],"titers,":[214],"reaching":[215],"750":[216],"mg/L":[217],"shake":[220],"flask":[221],"cultivation.":[222],"CONCLUSION:":[223],"approach,":[227],"identified":[229,252],"novel":[230],"without":[243],"compromising":[244],"growth":[246],"viability.":[248],"Most":[249],"not":[255],"previously":[256],"been":[257],"implicated":[258],"pathway":[261],"control.":[262],"Applying":[263],"same":[265],"using":[267],"different":[269],"starting":[270],"point":[271],"could":[272],"yield":[273],"alternative":[274],"sets":[275],"with":[278],"similar":[279],"or":[280],"outcome.":[282]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":14},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":9},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":8},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}