{"id":"https://openalex.org/W2031564595","doi":"https://doi.org/10.1104/pp.59.5.827","title":"Nitrogen Metabolism in Soybean Tissue Culture","display_name":"Nitrogen Metabolism in Soybean Tissue Culture","publication_year":1977,"publication_date":"1977-05-01","ids":{"openalex":"https://openalex.org/W2031564595","doi":"https://doi.org/10.1104/pp.59.5.827","mag":"2031564595","pmid":"https://pubmed.ncbi.nlm.nih.gov/16659950","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/543304"},"language":"en","primary_location":{"id":"doi:10.1104/pp.59.5.827","doi":"https://doi.org/10.1104/pp.59.5.827","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.59.5.827","pdf_url":"https://academic.oup.com/plphys/article-pdf/59/5/827/35580403/plphys_v59_5_827.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/plphys/article-pdf/59/5/827/35580403/plphys_v59_5_827.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111702499","display_name":"Joseph Carmine Polacco","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2799945547","display_name":"Connecticut Agricultural Experiment Station","ror":"https://ror.org/02t7c5797","country_code":"US","type":"government","lineage":["https://openalex.org/I2799945547"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Joseph Carmine Polacco","raw_affiliation_strings":["Department of Genetics, The Connecticut Agricultural Experiment Station, 123 Huntington Street, New Haven, Connecticut 06504"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, The Connecticut Agricultural Experiment Station, 123 Huntington Street, New Haven, Connecticut 06504","institution_ids":["https://openalex.org/I2799945547"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5111702499"],"corresponding_institution_ids":["https://openalex.org/I2799945547"],"apc_list":{"value":3851,"currency":"USD","value_usd":3851},"apc_paid":null,"fwci":14.5282,"has_fulltext":true,"cited_by_count":114,"citation_normalized_percentile":{"value":0.99364921,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"59","issue":"5","first_page":"827","last_page":"830"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.8417999744415283,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.8417999744415283,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.14880000054836273,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12566","display_name":"Nitrogen and Sulfur Effects on Brassica","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/1102","display_name":"Agronomy and Crop Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/soybean","display_name":"soybean","score":0.7570000290870667},{"id":"https://openalex.org/keywords/nitrogen-metabolism","display_name":"nitrogen metabolism","score":0.7089999914169312},{"id":"https://openalex.org/keywords/urea","display_name":"urea","score":0.6690000295639038},{"id":"https://openalex.org/keywords/urease","display_name":"urease","score":0.6830000281333923},{"id":"https://openalex.org/keywords/citrate-toxicity","display_name":"citrate toxicity","score":0.7689999938011169},{"id":"https://openalex.org/keywords/nickel","display_name":"nickel","score":0.7459999918937683},{"id":"https://openalex.org/keywords/divalent-cation-chelation","display_name":"divalent cation chelation","score":0.8930000066757202},{"id":"https://openalex.org/keywords/l-arginine","display_name":"L-arginine","score":0.6890000104904175},{"id":"https://openalex.org/keywords/ammonium-chloride","display_name":"ammonium chloride","score":0.8560000061988831},{"id":"https://openalex.org/keywords/magnesium","display_name":"magnesium","score":0.5590000152587891},{"id":"https://openalex.org/keywords/calcium","display_name":"calcium","score":0.9980000257492065},{"id":"https://openalex.org/keywords/cell-suspension-culture","display_name":"cell suspension culture","score":0.6470000147819519}],"concepts":[{"id":"https://openalex.org/C2780365088","wikidata":"https://www.wikidata.org/wiki/Q48318","display_name":"Urea","level":2,"score":0.8380935192108154},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6979662775993347},{"id":"https://openalex.org/C2780742167","wikidata":"https://www.wikidata.org/wiki/Q6460572","display_name":"Sodium citrate","level":2,"score":0.6858350038528442},{"id":"https://openalex.org/C2779521917","wikidata":"https://www.wikidata.org/wiki/Q22327086","display_name":"Urease","level":3,"score":0.6807789206504822},{"id":"https://openalex.org/C2779032678","wikidata":"https://www.wikidata.org/wiki/Q159683","display_name":"Citric acid","level":2,"score":0.6732327938079834},{"id":"https://openalex.org/C197404232","wikidata":"https://www.wikidata.org/wiki/Q319827","display_name":"Chelation","level":2,"score":0.5594602227210999},{"id":"https://openalex.org/C2780400767","wikidata":"https://www.wikidata.org/wiki/Q409728","display_name":"Trisodium citrate","level":2,"score":0.5483442544937134},{"id":"https://openalex.org/C74884574","wikidata":"https://www.wikidata.org/wiki/Q171407","display_name":"Divalent","level":2,"score":0.4840661585330963},{"id":"https://openalex.org/C2780919596","wikidata":"https://www.wikidata.org/wiki/Q575920","display_name":"Growth medium","level":3,"score":0.4678061306476593},{"id":"https://openalex.org/C517785266","wikidata":"https://www.wikidata.org/wiki/Q703","display_name":"Potassium","level":2,"score":0.467231810092926},{"id":"https://openalex.org/C111133373","wikidata":"https://www.wikidata.org/wiki/Q21132608","display_name":"Citrate synthase","level":3,"score":0.46571898460388184},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4623962342739105},{"id":"https://openalex.org/C13965031","wikidata":"https://www.wikidata.org/wiki/Q243545","display_name":"Nuclear chemistry","level":1,"score":0.4619242548942566},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.4288294315338135},{"id":"https://openalex.org/C204999636","wikidata":"https://www.wikidata.org/wiki/Q3602265","display_name":"ATP citrate lyase","level":4,"score":0.42497938871383667},{"id":"https://openalex.org/C179104552","wikidata":"https://www.wikidata.org/wiki/Q11165","display_name":"Inorganic chemistry","level":1,"score":0.33575206995010376},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2888495624065399},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.19374704360961914},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1385541558265686},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.1103537380695343},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1104/pp.59.5.827","doi":"https://doi.org/10.1104/pp.59.5.827","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.59.5.827","pdf_url":"https://academic.oup.com/plphys/article-pdf/59/5/827/35580403/plphys_v59_5_827.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},{"id":"pmid:16659950","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16659950","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant physiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:543304","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC543304/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1104/pp.59.5.827","doi":"https://doi.org/10.1104/pp.59.5.827","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.59.5.827","pdf_url":"https://academic.oup.com/plphys/article-pdf/59/5/827/35580403/plphys_v59_5_827.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.8409000039100647}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320324004","display_name":"Forskningsr\u00e5det om H\u00e4lsa, Arbetsliv och V\u00e4lf\u00e4rd","ror":"https://ror.org/02d290r06"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2031564595.pdf","grobid_xml":"https://content.openalex.org/works/W2031564595.grobid-xml"},"referenced_works_count":10,"referenced_works":["https://openalex.org/W1498995552","https://openalex.org/W1967118561","https://openalex.org/W1983580216","https://openalex.org/W1984505392","https://openalex.org/W2007714208","https://openalex.org/W2014763530","https://openalex.org/W2024556021","https://openalex.org/W2029120139","https://openalex.org/W2031474232","https://openalex.org/W4296535077"],"related_works":["https://openalex.org/W2042762365","https://openalex.org/W2075079436","https://openalex.org/W2031588168","https://openalex.org/W2031474232","https://openalex.org/W4231430728","https://openalex.org/W2166257060","https://openalex.org/W1979186284","https://openalex.org/W2073088419","https://openalex.org/W1964463148","https://openalex.org/W2166260230"],"abstract_inverted_index":{"Potassium":[0],"citrate":[1,23,65,83,156,173,177,198,216],"(10":[2,44],"mM,":[3],"pH":[4],"6)":[5],"inhibits":[6],"the":[7,18,32,69,88,127,209,220,227,256],"growth":[8,33,66,71,77,103,110,207],"of":[9,42,53,58,90,96,129,163,183,211,229,235,259],"cultured":[10],"(Glycine":[11],"max":[12],"L.)":[13],"cells":[14,118,240,247],"when":[15,111],"urea":[16,230,251],"is":[17,25,72,113,138,146,160,178,189,223],"sole":[19,114],"nitrogen":[20,115,252],"source.":[21,116],"Ureadependent":[22],"toxicity":[24,174,199,217],"overcome":[26,64],"by":[27,180],"three":[28],"separate":[29],"additions":[30],"to":[31,140,231],"medium:":[34],"(a)":[35],"NH(4)Cl":[36],"(20":[37],"mM);":[38,49],"(b)":[39],"high":[40],"levels":[41,52,95,237],"MgCl(2)":[43],"mM)":[45,99,263],"or":[46,169],"CaCl(2)":[47],"(5-10":[48],"(c)":[50],"low":[51,94],"NiSO(4)":[54,61,97,137],"(10(-2)":[55],"mM).":[56],"Additions":[57],"10(-2)":[59,135,194],"mM":[60,131,136,195,203],"not":[62,108],"only":[63],"inhibition":[67],"but":[68],"resultant":[70],"usually":[73],"better":[74],"than":[75],"urea-supported":[76,102,206],"in":[78,104,126,151,208,238],"basal":[79,152,244],"medium":[80,153],"(neither":[81],"added":[82,85,91,139,158],"nor":[84],"nickel).":[86],"In":[87],"absence":[89,210],"citrate,":[92],"exceedingly":[93],"(10(-4)":[98],"strongly":[100],"stimulate":[101],"suspension":[105],"cultures.Citrate":[106],"does":[107],"inhibit":[109],"arginine":[112,120],"However,":[117],"using":[119],"have":[121],"no":[122],"net":[123],"urease":[124,143,236],"synthesis":[125],"presence":[128],"10":[130,147],"potassium":[132],"citrate.":[133,212],"When":[134],"this":[141,188,242],"medium,":[142,245],"specific":[144],"activity":[145],"times":[148],"that":[149,176,219],"observed":[150],"lacking":[154],"both":[155],"and":[157,200],"nickel.Citrate":[159],"a":[161],"chelator":[162],"divalent":[164,186],"cations.":[165],"That":[166,213],"additional":[167],"Mg(2+)":[168],"Ca(2+)":[170],"alleviates":[171],"urea-dependent":[172],"indicates":[175,218],"acting":[179],"chelation,":[181],"probably":[182,190],"another":[184],"trace":[185,221,257],"cation;":[187],"Ni(2+)":[191,222,233,260],"since":[192],"at":[193,201],"it":[196,204],"overcomes":[197,215],"10(-4)":[202],"stimulates":[205],"ammonia":[214],"essential":[224],"specifically":[225],"for":[226],"conversion":[228],"ammonia.":[232],"stimulation":[234],"arginine-grown":[239],"supports":[241],"contention.In":[243],"soybean":[246],"grow":[248],"slowly":[249],"with":[250],"source":[253],"presumably":[254],"because":[255],"amounts":[258],"present":[261],"(</=10(-6)":[262],"are":[264],"growth-limiting.":[265]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2017,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}