{"id":"https://openalex.org/W2031130822","doi":"https://doi.org/10.1021/bi020200i","title":"Identification of a Peroxide-Sensitive Redox Switch at the CXXC Motif in the Human Mitochondrial Branched Chain Aminotransferase","display_name":"Identification of a Peroxide-Sensitive Redox Switch at the CXXC Motif in the Human Mitochondrial Branched Chain Aminotransferase","publication_year":2002,"publication_date":"2002-06-21","ids":{"openalex":"https://openalex.org/W2031130822","doi":"https://doi.org/10.1021/bi020200i","mag":"2031130822","pmid":"https://pubmed.ncbi.nlm.nih.gov/12119021"},"language":"en","primary_location":{"id":"doi:10.1021/bi020200i","doi":"https://doi.org/10.1021/bi020200i","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi020200i","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://eprints.uwe.ac.uk/7942/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5065253650","display_name":"Myra E. Conway","orcid":"https://orcid.org/0000-0001-6801-7245","observed_orcids":["https://orcid.org/0000-0001-6801-7245"]},"institutions":[{"id":"https://openalex.org/I130769515","display_name":"Pennsylvania State University","ror":"https://ror.org/04p491231","country_code":"US","type":"education","lineage":["https://openalex.org/I130769515"]},{"id":"https://openalex.org/I4405269449","display_name":"Wake Forest University Health Sciences","ror":"https://ror.org/046r1r105","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4405269449","https://openalex.org/I47251452"]},{"id":"https://openalex.org/I47251452","display_name":"Wake Forest University","ror":"https://ror.org/0207ad724","country_code":"US","type":"education","lineage":["https://openalex.org/I47251452"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Myra E. Conway","raw_affiliation_strings":["Department of Biochemistry and Department of Internal Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, and Department of Biochemistry and Molecular Biology, Althouse Lab, Pennsylvania State University, University Park, Pennsylvania 16802"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Department of Internal Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, and Department of Biochemistry and Molecular Biology, Althouse Lab, Pennsylvania State University, University Park, Pennsylvania 16802","institution_ids":["https://openalex.org/I130769515","https://openalex.org/I4405269449","https://openalex.org/I47251452"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006590145","display_name":"Neela H. Yennawar","orcid":"https://orcid.org/0000-0001-7278-659X","observed_orcids":["https://orcid.org/0000-0001-7278-659X"]},"institutions":[{"id":"https://openalex.org/I130769515","display_name":"Pennsylvania State University","ror":"https://ror.org/04p491231","country_code":"US","type":"education","lineage":["https://openalex.org/I130769515"]},{"id":"https://openalex.org/I4405269449","display_name":"Wake Forest University Health Sciences","ror":"https://ror.org/046r1r105","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4405269449","https://openalex.org/I47251452"]},{"id":"https://openalex.org/I47251452","display_name":"Wake Forest University","ror":"https://ror.org/0207ad724","country_code":"US","type":"education","lineage":["https://openalex.org/I47251452"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Neela Yennawar","raw_affiliation_strings":["Department of Biochemistry and Department of Internal Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, and Department of Biochemistry and Molecular Biology, Althouse Lab, Pennsylvania State University, University Park, Pennsylvania 16802"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Department of Internal Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, and Department of Biochemistry and Molecular Biology, Althouse Lab, Pennsylvania State University, University Park, Pennsylvania 16802","institution_ids":["https://openalex.org/I130769515","https://openalex.org/I4405269449","https://openalex.org/I47251452"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023692478","display_name":"Reidar Wallin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130769515","display_name":"Pennsylvania State University","ror":"https://ror.org/04p491231","country_code":"US","type":"education","lineage":["https://openalex.org/I130769515"]},{"id":"https://openalex.org/I4405269449","display_name":"Wake Forest University Health Sciences","ror":"https://ror.org/046r1r105","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4405269449","https://openalex.org/I47251452"]},{"id":"https://openalex.org/I47251452","display_name":"Wake Forest University","ror":"https://ror.org/0207ad724","country_code":"US","type":"education","lineage":["https://openalex.org/I47251452"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Reidar Wallin","raw_affiliation_strings":["Department of Biochemistry and Department of Internal Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, and Department of Biochemistry and Molecular Biology, Althouse Lab, Pennsylvania State University, University Park, Pennsylvania 16802"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Department of Internal Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, and Department of Biochemistry and Molecular Biology, Althouse Lab, Pennsylvania State University, University Park, Pennsylvania 16802","institution_ids":["https://openalex.org/I130769515","https://openalex.org/I4405269449","https://openalex.org/I47251452"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039556554","display_name":"Leslie B. Poole","orcid":"https://orcid.org/0000-0003-0334-7328","observed_orcids":["https://orcid.org/0000-0003-0334-7328"]},"institutions":[{"id":"https://openalex.org/I130769515","display_name":"Pennsylvania State University","ror":"https://ror.org/04p491231","country_code":"US","type":"education","lineage":["https://openalex.org/I130769515"]},{"id":"https://openalex.org/I4405269449","display_name":"Wake Forest University Health Sciences","ror":"https://ror.org/046r1r105","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4405269449","https://openalex.org/I47251452"]},{"id":"https://openalex.org/I47251452","display_name":"Wake Forest University","ror":"https://ror.org/0207ad724","country_code":"US","type":"education","lineage":["https://openalex.org/I47251452"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Leslie B. Poole","raw_affiliation_strings":["Department of Biochemistry and Department of Internal Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, and Department of Biochemistry and Molecular Biology, Althouse Lab, Pennsylvania State University, University Park, Pennsylvania 16802"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Department of Internal Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, and Department of Biochemistry and Molecular Biology, Althouse Lab, Pennsylvania State University, University Park, Pennsylvania 16802","institution_ids":["https://openalex.org/I130769515","https://openalex.org/I4405269449","https://openalex.org/I47251452"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108603441","display_name":"Susan M. Hutson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130769515","display_name":"Pennsylvania State University","ror":"https://ror.org/04p491231","country_code":"US","type":"education","lineage":["https://openalex.org/I130769515"]},{"id":"https://openalex.org/I4405269449","display_name":"Wake Forest University Health Sciences","ror":"https://ror.org/046r1r105","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4405269449","https://openalex.org/I47251452"]},{"id":"https://openalex.org/I47251452","display_name":"Wake Forest University","ror":"https://ror.org/0207ad724","country_code":"US","type":"education","lineage":["https://openalex.org/I47251452"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Susan M. Hutson","raw_affiliation_strings":["Department of Biochemistry and Department of Internal Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, and Department of Biochemistry and Molecular Biology, Althouse Lab, Pennsylvania State University, University Park, Pennsylvania 16802"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Department of Internal Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, and Department of Biochemistry and Molecular Biology, Althouse Lab, Pennsylvania State University, University Park, Pennsylvania 16802","institution_ids":["https://openalex.org/I130769515","https://openalex.org/I4405269449","https://openalex.org/I47251452"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.9387,"has_fulltext":false,"cited_by_count":46,"citation_normalized_percentile":{"value":0.69739744,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"41","issue":"29","first_page":"9070","last_page":"9078"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.5029000043869019,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.5029000043869019,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.25270000100135803,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.0982000008225441,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/branched-chain-aminotransferase","display_name":"branched-chain amino acid aminotransferase","score":0.953000009059906},{"id":"https://openalex.org/keywords/mitochondrial-isoenzyme","display_name":"mitochondrial isoenzyme","score":0.7239999771118164},{"id":"https://openalex.org/keywords/cxxc-motif","display_name":"CXXC motif","score":0.8330000042915344},{"id":"https://openalex.org/keywords/redox-switch","display_name":"redox switch","score":0.9179999828338623},{"id":"https://openalex.org/keywords/disulfide-bond-formation","display_name":"disulfide bond formation","score":0.9100000262260437},{"id":"https://openalex.org/keywords/reactive-cysteine-residues","display_name":"reactive cysteine residues","score":0.7730000019073486},{"id":"https://openalex.org/keywords/hydrogen-peroxide-sensitivity","display_name":"hydrogen peroxide sensitivity","score":0.7599999904632568},{"id":"https://openalex.org/keywords/sulfhydryl-reagent-inhibition","display_name":"sulfhydryl reagent inhibition","score":0.796999990940094},{"id":"https://openalex.org/keywords/enzyme-regulation","display_name":"enzyme regulation","score":0.4519999921321869},{"id":"https://openalex.org/keywords/branched-chain-amino-acid-metabolism","display_name":"branched-chain amino acid metabolism","score":0.9430000185966492},{"id":"https://openalex.org/keywords/protein-oxidation","display_name":"protein oxidation","score":0.34700000286102295}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.871246337890625},{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.8166812658309937},{"id":"https://openalex.org/C2777824588","wikidata":"https://www.wikidata.org/wiki/Q414783","display_name":"Dithiothreitol","level":3,"score":0.7807619571685791},{"id":"https://openalex.org/C40875361","wikidata":"https://www.wikidata.org/wiki/Q2356542","display_name":"Reagent","level":2,"score":0.6528716683387756},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.6503891944885254},{"id":"https://openalex.org/C55904794","wikidata":"https://www.wikidata.org/wiki/Q82682","display_name":"Redox","level":2,"score":0.6122652888298035},{"id":"https://openalex.org/C40684141","wikidata":"https://www.wikidata.org/wiki/Q902061","display_name":"Electrospray ionization","level":3,"score":0.561306357383728},{"id":"https://openalex.org/C2781324293","wikidata":"https://www.wikidata.org/wiki/Q220410","display_name":"Thiol","level":2,"score":0.5123942494392395},{"id":"https://openalex.org/C2780105360","wikidata":"https://www.wikidata.org/wiki/Q6743002","display_name":"Maleimide","level":2,"score":0.4949924349784851},{"id":"https://openalex.org/C186423591","wikidata":"https://www.wikidata.org/wiki/Q216830","display_name":"Reducing agent","level":2,"score":0.4933744966983795},{"id":"https://openalex.org/C77176794","wikidata":"https://www.wikidata.org/wiki/Q187689","display_name":"Oxidizing agent","level":2,"score":0.4823122024536133},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.4680314362049103},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4592883288860321},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.45864373445510864},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4136120080947876},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.3166552186012268},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.1655770242214203},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.10732975602149963}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000637","descriptor_name":"Transaminases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000637","descriptor_name":"Transaminases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008928","descriptor_name":"Mitochondria","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010545","descriptor_name":"Peroxides","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013438","descriptor_name":"Sulfhydryl Compounds","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020816","descriptor_name":"Amino Acid Motifs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D029701","descriptor_name":"Saccharomyces cerevisiae Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1021/bi020200i","doi":"https://doi.org/10.1021/bi020200i","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi020200i","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:12119021","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12119021","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.384.408","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.384.408","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://scbtrack.wfu.edu/Poole-files/bi020200i.pdf","raw_type":"text"},{"id":"pmh:oai:eprints.uwe.ac.uk:7942","doi":null,"is_oa":true,"landing_page_url":"http://eprints.uwe.ac.uk/7942/","pdf_url":null,"source":{"id":"https://openalex.org/S4306401464","display_name":"UWE Research Repository (UWE Bristol)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I178535277","host_organization_name":"University of the West of England","host_organization_lineage":["https://openalex.org/I178535277"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Article"},{"id":"pmh:oai:uwe-repository.worktribe.com:1082436","doi":null,"is_oa":true,"landing_page_url":"https://uwe-repository.worktribe.com/output/1082436","pdf_url":null,"source":{"id":"https://openalex.org/S4306401464","display_name":"UWE Research Repository (UWE Bristol)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I178535277","host_organization_name":"University of the West of England","host_organization_lineage":["https://openalex.org/I178535277"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:eprints.uwe.ac.uk:7942","doi":null,"is_oa":true,"landing_page_url":"http://eprints.uwe.ac.uk/7942/","pdf_url":null,"source":{"id":"https://openalex.org/S4306401464","display_name":"UWE Research Repository (UWE Bristol)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I178535277","host_organization_name":"University of the West of England","host_organization_lineage":["https://openalex.org/I178535277"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4366278463","display_name":"Mechanistic Studies of Alkyl Hydroperoxide Reductase and Related Redox Proteins","funder_award_id":"R01GM050389","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G7435064558","display_name":"Branched Chain Amino Acid Metabolism","funder_award_id":"R01DK034738","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1484322088","https://openalex.org/W1494772917","https://openalex.org/W1546351395","https://openalex.org/W1603747702","https://openalex.org/W1791153000","https://openalex.org/W1908039135","https://openalex.org/W2001641653","https://openalex.org/W2019518202","https://openalex.org/W2063444740","https://openalex.org/W2069813005","https://openalex.org/W2073417483","https://openalex.org/W2119665261","https://openalex.org/W2140033230","https://openalex.org/W2171853694"],"related_works":["https://openalex.org/W7099982341","https://openalex.org/W2061794989","https://openalex.org/W7073630933","https://openalex.org/W1601983175","https://openalex.org/W7097015210","https://openalex.org/W7019688980","https://openalex.org/W2998577147","https://openalex.org/W2156484159","https://openalex.org/W2083262521","https://openalex.org/W2119293237"],"abstract_inverted_index":{"The":[0,87],"human":[1],"mitochondrial":[2],"branched":[3,269],"chain":[4,270],"aminotransferase":[5],"isoenzyme":[6],"(hBCATm)":[7],"must":[8],"be":[9],"stored":[10],"in":[11,27,73,143,165,191,213,262,265],"a":[12,93,110,126,136,162,192,248],"reducing":[13],"environment":[14],"to":[15,46,133,149],"remain":[16],"active.":[17],"Oxidation":[18],"or":[19],"labeling":[20],"of":[21,44,77,114,128,176,195,204,216,226,244,268],"hBCATm":[22,45,53,180,205,245],"with":[23,206],"sulfhydryl":[24],"reagents":[25],"results":[26,237],"enzyme":[28],"inhibition.":[29],"In":[30,49,131],"this":[31,258],"study,":[32],"we":[33],"investigated":[34],"both":[35],"the":[36,42,74,78,84,115,119,144,150,196,214,230,236,266],"structural":[37,137],"and":[38,64,99,172,211,223,232],"biochemical":[39],"basis":[40],"for":[41,92],"sensitivity":[43],"these":[47,104,157],"reagents.":[48],"its":[50,209],"native":[51],"form,":[52],"has":[54,260],"two":[55],"reactive":[56],"cysteine":[57,105,158],"residues":[58,106,159],"which":[59,142],"were":[60],"identified":[61],"as":[62,161],"Cys315":[63,98,134,222],"Cys318":[65],"using":[66],"iodinated":[67],"beta-(4-hydroxyphenyl)ethyl":[68],"maleimide.":[69],"These":[70],"are":[71],"located":[72],"large":[75],"domain":[76],"homodimer,":[79],"about":[80],"10":[81],"A":[82],"from":[83],"active":[85,151],"site.":[86],"crystal":[88],"structures":[89],"show":[90],"evidence":[91,154],"thiol-thiolate":[94],"hydrogen":[95],"bond":[96,112,188,220],"between":[97,118,221],"Cys318.":[100,224],"Under":[101],"oxidizing":[102],"conditions,":[103],"can":[107],"reasonably":[108],"form":[109,146],"disulfide":[111,187,219],"because":[113],"short":[116],"distance":[117],"sulfur":[120],"atoms":[121],"(3.09-3.46":[122],"A),":[123],"requiring":[124],"only":[125],"decrease":[127],"1.1-1.5":[129],"A.":[130],"addition":[132],"playing":[135],"role":[138,264],"by":[139,247],"anchoring":[140],"Tyr173,":[141],"ketimine":[145],"increases":[147],"access":[148],"site,":[152],"our":[153],"indicates":[155],"that":[156,182,202,239],"act":[160],"redox":[163],"switch":[164],"hBCATm.":[166],"Electrospray":[167],"ionization":[168],"mass":[169],"spectrometry":[170],"analysis":[171],"UV-Vis":[173],"spectroscopic":[174],"studies":[175,254],"5,5'-dithiobis(2-nitrobenzoic":[177],"acid)":[178],"labeled":[179],"showed":[181],"during":[183],"labeling,":[184],"an":[185,217,261],"intrasubunit":[186,218],"was":[189,200],"formed":[190],"significant":[193],"portion":[194],"protein.":[197],"Furthermore,":[198],"it":[199],"established":[201],"reaction":[203],"H2O2":[207],"abolished":[208],"activity":[210,246],"resulted":[212],"formation":[215],"Addition":[225],"dithiothreitol":[227],"completely":[228],"reversed":[229],"oxidation":[231],"restored":[233],"activity.":[234],"Therefore,":[235],"demonstrate":[238],"there":[240],"is":[241],"redox-linked":[242],"regulation":[243,267],"peroxide":[249],"sensitive":[250],"CXXC":[251],"center.":[252],"Future":[253],"will":[255],"determine":[256],"if":[257],"center":[259],"vivo":[263],"amino":[271],"acid":[272],"metabolism.":[273]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":3},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":6},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}