{"id":"https://openalex.org/W2030733655","doi":"https://doi.org/10.1111/j.1399-3011.1995.tb01323.x","title":"Identification and characterization of a novel synthetic peptide substrate specific for Src\u2010family protein tyrosine kinases","display_name":"Identification and characterization of a novel synthetic peptide substrate specific for Src\u2010family protein tyrosine kinases","publication_year":1995,"publication_date":"1995-06-01","ids":{"openalex":"https://openalex.org/W2030733655","doi":"https://doi.org/10.1111/j.1399-3011.1995.tb01323.x","mag":"2030733655","pmid":"https://pubmed.ncbi.nlm.nih.gov/7558590"},"language":"en","primary_location":{"id":"doi:10.1111/j.1399-3011.1995.tb01323.x","doi":"https://doi.org/10.1111/j.1399-3011.1995.tb01323.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1399-3011.1995.tb01323.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210190181","display_name":"International journal of peptide & protein research","issn_l":"0367-8377","issn":["0367-8377"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Peptide and Protein Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016566536","display_name":"Kit S. Lam","orcid":"https://orcid.org/0000-0002-3076-6969","observed_orcids":["https://orcid.org/0000-0002-3076-6969"]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]},{"id":"https://openalex.org/I4390039223","display_name":"University of Arizona Cancer Center","ror":"https://ror.org/04tvx8690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039223"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"KIT S. LAM","raw_affiliation_strings":["Arizona Cancer Center, University of Arizona, Tucson, USA","Arizona Cancer Center and Department of Medicine, University of Arizona, Tucson, Arizona","Jinzi Wu Selectide Corporation 1580 E. Hanley Boulevard Tucson, AZ 85737 USA","Kit S . Lam Arizona Cancer Center and Department of Medicine Universit) of Arizona 1515 N. Campbell Avenue Tucson, AZ 85724 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Arizona Cancer Center, University of Arizona, Tucson, USA","institution_ids":["https://openalex.org/I138006243","https://openalex.org/I4390039223"]},{"raw_affiliation_string":"Arizona Cancer Center and Department of Medicine, University of Arizona, Tucson, Arizona","institution_ids":["https://openalex.org/I138006243","https://openalex.org/I4390039223"]},{"raw_affiliation_string":"Jinzi Wu Selectide Corporation 1580 E. Hanley Boulevard Tucson, AZ 85737 USA","institution_ids":[]},{"raw_affiliation_string":"Kit S . Lam Arizona Cancer Center and Department of Medicine Universit) of Arizona 1515 N. Campbell Avenue Tucson, AZ 85724 USA","institution_ids":["https://openalex.org/I138006243","https://openalex.org/I4390039223"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038683160","display_name":"Jinzi J. Wu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]},{"id":"https://openalex.org/I4390039223","display_name":"University of Arizona Cancer Center","ror":"https://ror.org/04tvx8690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039223"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"JINZI WU","raw_affiliation_strings":["Jinzi Wu Selectide Corporation 1580 E. Hanley Boulevard Tucson, AZ 85737 USA","Kit S . Lam Arizona Cancer Center and Department of Medicine Universit) of Arizona 1515 N. Campbell Avenue Tucson, AZ 85724 USA","Selectide Corporation, Tucson, Arizona, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Jinzi Wu Selectide Corporation 1580 E. Hanley Boulevard Tucson, AZ 85737 USA","institution_ids":[]},{"raw_affiliation_string":"Kit S . Lam Arizona Cancer Center and Department of Medicine Universit) of Arizona 1515 N. Campbell Avenue Tucson, AZ 85724 USA","institution_ids":["https://openalex.org/I138006243","https://openalex.org/I4390039223"]},{"raw_affiliation_string":"Selectide Corporation, Tucson, Arizona, USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5044298887","display_name":"Qiang Lou","orcid":"https://orcid.org/0000-0002-8253-0691","observed_orcids":["https://orcid.org/0000-0002-8253-0691"]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]},{"id":"https://openalex.org/I4390039223","display_name":"University of Arizona Cancer Center","ror":"https://ror.org/04tvx8690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039223"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"QIANG LOU","raw_affiliation_strings":["Arizona Cancer Center and Department of Medicine, University of Arizona, Tucson, Arizona"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Arizona Cancer Center and Department of Medicine, University of Arizona, Tucson, Arizona","institution_ids":["https://openalex.org/I138006243","https://openalex.org/I4390039223"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5016566536","https://openalex.org/A5038683160"],"corresponding_institution_ids":["https://openalex.org/I138006243","https://openalex.org/I4390039223"],"apc_list":null,"apc_paid":null,"fwci":1.9713,"has_fulltext":false,"cited_by_count":79,"citation_normalized_percentile":{"value":0.87223532,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"45","issue":"6","first_page":"587","last_page":"592"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.8339999914169312,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.8339999914169312,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.10930000245571136,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12104","display_name":"Protein Tyrosine Phosphatases","score":0.025499999523162842,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/src-family-kinases","display_name":"Src family kinases","score":0.9340000152587891},{"id":"https://openalex.org/keywords/p60c-src","display_name":"p60c-src","score":0.9269999861717224},{"id":"https://openalex.org/keywords/peptide-substrates","display_name":"peptide substrates","score":0.6010000109672546},{"id":"https://openalex.org/keywords/tyrosine-phosphorylation-sites","display_name":"tyrosine phosphorylation sites","score":0.7409999966621399},{"id":"https://openalex.org/keywords/kinase-specificity","display_name":"kinase specificity","score":0.5870000123977661},{"id":"https://openalex.org/keywords/substrate-identification","display_name":"substrate identification","score":0.46399998664855957},{"id":"https://openalex.org/keywords/combinatorial-peptide-libraries","display_name":"combinatorial peptide libraries","score":0.8460000157356262},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.41200000047683716}],"concepts":[{"id":"https://openalex.org/C183141693","wikidata":"https://www.wikidata.org/wiki/Q5005962","display_name":"Tyrosine-protein kinase CSK","level":5,"score":0.8070869445800781},{"id":"https://openalex.org/C108636557","wikidata":"https://www.wikidata.org/wiki/Q21109354","display_name":"Proto-oncogene tyrosine-protein kinase Src","level":3,"score":0.7759276032447815},{"id":"https://openalex.org/C196347352","wikidata":"https://www.wikidata.org/wiki/Q24771787","display_name":"SH3 domain","level":4,"score":0.6548255681991577},{"id":"https://openalex.org/C42362537","wikidata":"https://www.wikidata.org/wiki/Q87099740","display_name":"Tyrosine kinase","level":3,"score":0.5969738364219666},{"id":"https://openalex.org/C197153747","wikidata":"https://www.wikidata.org/wiki/Q24780316","display_name":"SH2 domain","level":4,"score":0.5409079790115356},{"id":"https://openalex.org/C85528070","wikidata":"https://www.wikidata.org/wiki/Q643602","display_name":"Protein tyrosine phosphatase","level":3,"score":0.524699330329895},{"id":"https://openalex.org/C101544691","wikidata":"https://www.wikidata.org/wiki/Q420511","display_name":"Receptor tyrosine kinase","level":3,"score":0.4987659454345703},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.497609406709671},{"id":"https://openalex.org/C2776165026","wikidata":"https://www.wikidata.org/wiki/Q188017","display_name":"Tyrosine","level":2,"score":0.49511733651161194},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.4810948073863983},{"id":"https://openalex.org/C120504264","wikidata":"https://www.wikidata.org/wiki/Q1147372","display_name":"Cyclin-dependent kinase 1","level":4,"score":0.4486989676952362},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.4411037266254425},{"id":"https://openalex.org/C2777553839","wikidata":"https://www.wikidata.org/wiki/Q14330986","display_name":"Tyrosine phosphorylation","level":3,"score":0.44041356444358826},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4151943027973175},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3801138401031494},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.15536996722221375},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.0},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005810","descriptor_name":"Multigene Family","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016203","descriptor_name":"CDC2 Protein Kinase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D016391","descriptor_name":"Genes, src","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016392","descriptor_name":"Proto-Oncogene Proteins pp60(c-src)","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1399-3011.1995.tb01323.x","doi":"https://doi.org/10.1111/j.1399-3011.1995.tb01323.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1399-3011.1995.tb01323.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210190181","display_name":"International journal of peptide & protein research","issn_l":"0367-8377","issn":["0367-8377"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Peptide and Protein Research","raw_type":"journal-article"},{"id":"pmid:7558590","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7558590","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of peptide and protein research","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1628675125","display_name":null,"funder_award_id":"CA13074","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G3494388860","display_name":"Therapeutic Targeting of Hypoxic and Oxidative Stress","funder_award_id":"P01CA017094","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G4205871284","display_name":"DISCOVERY OF PEPTIDE ANTICANCER DRUGS","funder_award_id":"U01CA057723","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W938256215","https://openalex.org/W1493020321","https://openalex.org/W1504478980","https://openalex.org/W1881961370","https://openalex.org/W1966870645","https://openalex.org/W1967234833","https://openalex.org/W1987262939","https://openalex.org/W1996427305","https://openalex.org/W1999450178","https://openalex.org/W2000008651","https://openalex.org/W2004686233","https://openalex.org/W2006446936","https://openalex.org/W2007745785","https://openalex.org/W2010649076","https://openalex.org/W2015314915","https://openalex.org/W2020257412","https://openalex.org/W2024308660","https://openalex.org/W2032062053","https://openalex.org/W2041719513","https://openalex.org/W2048721372","https://openalex.org/W2065236789","https://openalex.org/W2072009508","https://openalex.org/W2080262597","https://openalex.org/W2084429764","https://openalex.org/W2127728110","https://openalex.org/W2235427389","https://openalex.org/W2313498935","https://openalex.org/W2416661434","https://openalex.org/W4297025036"],"related_works":["https://openalex.org/W2030062905","https://openalex.org/W2050996539","https://openalex.org/W611354118","https://openalex.org/W2071542297","https://openalex.org/W2142394765","https://openalex.org/W2053139373","https://openalex.org/W1996508948","https://openalex.org/W1493020321","https://openalex.org/W1524153003","https://openalex.org/W2044938706"],"abstract_inverted_index":{"Using":[0],"a":[1,20,27,64,77,97,111],"random":[2],"combinatorial":[3],"peptide":[4,65],"library":[5],"method":[6],"[Wu,":[7],"J.,":[8],"Ma,":[9],"Q.":[10],"N.":[11],"&":[12],"Lam,":[13],"K.":[14],"S.":[15],"(1994)":[16],"Biochemistry":[17],"33,":[18],"14825-14833]":[19],"novel":[21],"peptide,":[22],"YIYGSFK,":[23],"was":[24,43,57],"identified":[25],"as":[26,96],"substrate":[28,81,98,117],"for":[29,55,82,99,118],"p60c-src":[30,56],"protein":[31,85,104,121],"tyrosine":[32,86,105,122],"kinase.":[33],"Mass":[34],"spectrometric":[35],"analysis":[36],"showed":[37,49],"that":[38,50,108],"tyrosine-3":[39],"from":[40,67],"the":[41,44,51,83,90,119],"N-terminus":[42],"phosphorylation":[45],"site.":[46],"Kinetic":[47],"studies":[48],"Km":[52],"of":[53,89,92],"YIYGSFK":[54,93,109],"55":[58],"microM,":[59],"about":[60],"6.4-fold":[61],"lower":[62],"than":[63],"derived":[66],"p34cdc2":[68],"[cdc2(6-20),":[69],"KVEKIGEGTYGVVYK],":[70],"which":[71],"had":[72],"been":[73],"reported":[74],"to":[75],"be":[76],"specific":[78,114],"and":[79,94,102,115],"efficient":[80,116],"Src-family":[84,101,120],"kinases.":[87,123],"Comparison":[88],"specificity":[91],"cdc2(6-20)":[95],"various":[100],"non-Src-family":[103],"kinases":[106],"suggests":[107],"is":[110],"much":[112],"more":[113]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}