{"id":"https://openalex.org/W2030476795","doi":"https://doi.org/10.1016/s0014-5793(97)00132-4","title":"In seedlings of the heavy metal accumulator Brassica juncea Cu2+ differentially affects transcript amounts for \u03b3\u2010glutamylcysteine synthetase (\u03b3\u2010ECS) and metallothionein (MT2)","display_name":"In seedlings of the heavy metal accumulator Brassica juncea Cu2+ differentially affects transcript amounts for \u03b3\u2010glutamylcysteine synthetase (\u03b3\u2010ECS) and metallothionein (MT2)","publication_year":1997,"publication_date":"1997-03-10","ids":{"openalex":"https://openalex.org/W2030476795","doi":"https://doi.org/10.1016/s0014-5793(97)00132-4","mag":"2030476795","pmid":"https://pubmed.ncbi.nlm.nih.gov/9119067"},"language":"en","primary_location":{"id":"doi:10.1016/s0014-5793(97)00132-4","doi":"https://doi.org/10.1016/s0014-5793(97)00132-4","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0014-5793(97)00132-4","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2897%2900132-4","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2897%2900132-4","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081887603","display_name":"Holger Sch\u00e4fer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Holger J Sch\u00e4fer","raw_affiliation_strings":["Botanisches Institut, Heidelberg, Germany","Botanisches Institut, Im Neuenheimer Feld 360, D-69120 Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Botanisches Institut, Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]},{"raw_affiliation_string":"Botanisches Institut, Im Neuenheimer Feld 360, D-69120 Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111889403","display_name":"Steffen Greiner","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Steffen Greiner","raw_affiliation_strings":["Botanisches Institut, Im Neuenheimer Feld 360, D-69120 Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Botanisches Institut, Im Neuenheimer Feld 360, D-69120 Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101632427","display_name":"Thomas Rausch","orcid":"https://orcid.org/0000-0001-5200-9857","observed_orcids":["https://orcid.org/0000-0001-5200-9857"]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Thomas Rausch","raw_affiliation_strings":["Botanisches Institut, Im Neuenheimer Feld 360, D-69120 Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Botanisches Institut, Im Neuenheimer Feld 360, D-69120 Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5048512948","display_name":"Angela Haag-Kerwer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Angela Haag-Kerwer","raw_affiliation_strings":["Botanisches Institut, Im Neuenheimer Feld 360, D-69120 Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Botanisches Institut, Im Neuenheimer Feld 360, D-69120 Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5048512948"],"corresponding_institution_ids":["https://openalex.org/I223822909"],"apc_list":{"value":3980,"currency":"USD","value_usd":3980},"apc_paid":null,"fwci":6.5107,"has_fulltext":true,"cited_by_count":83,"citation_normalized_percentile":{"value":0.96427493,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"404","issue":"2-3","first_page":"216","last_page":"220"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.5898000001907349,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.5898000001907349,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12566","display_name":"Nitrogen and Sulfur Effects on Brassica","score":0.14650000631809235,"subfield":{"id":"https://openalex.org/subfields/1102","display_name":"Agronomy and Crop Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10139","display_name":"Heavy metals in environment","score":0.0617000013589859,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/brassica-juncea","display_name":"Brassica juncea","score":0.8820000290870667},{"id":"https://openalex.org/keywords/gamma-glutamylcysteine-synthetase","display_name":"gamma-glutamylcysteine synthetase","score":0.9810000061988831},{"id":"https://openalex.org/keywords/metallothionein-2","display_name":"metallothionein-2","score":0.9729999899864197},{"id":"https://openalex.org/keywords/glutathione-synthesis","display_name":"glutathione synthesis","score":0.9580000042915344},{"id":"https://openalex.org/keywords/phytochelatins","display_name":"phytochelatins","score":0.9940000176429749},{"id":"https://openalex.org/keywords/heavy-metal-accumulation","display_name":"heavy metal accumulation","score":0.8230000138282776},{"id":"https://openalex.org/keywords/copper-stress","display_name":"copper stress","score":0.6909999847412109},{"id":"https://openalex.org/keywords/cadmium-stress","display_name":"cadmium stress","score":0.9539999961853027},{"id":"https://openalex.org/keywords/zinc-stress","display_name":"zinc stress","score":0.9919999837875366},{"id":"https://openalex.org/keywords/gene-expression-regulation","display_name":"gene expression regulation","score":0.8289999961853027},{"id":"https://openalex.org/keywords/seedling-growth","display_name":"seedling growth","score":0.6470000147819519},{"id":"https://openalex.org/keywords/metal-sequestration","display_name":"metal sequestration","score":0.48500001430511475}],"concepts":[{"id":"https://openalex.org/C2776747608","wikidata":"https://www.wikidata.org/wiki/Q58677","display_name":"Brassica","level":2,"score":0.8159064054489136},{"id":"https://openalex.org/C20719415","wikidata":"https://www.wikidata.org/wiki/Q416896","display_name":"Metallothionein","level":3,"score":0.7767045497894287},{"id":"https://openalex.org/C544153396","wikidata":"https://www.wikidata.org/wiki/Q11426","display_name":"Metal","level":2,"score":0.5764797925949097},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5485683679580688},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.42026543617248535},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3680828809738159},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3389163613319397},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13627800345420837},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001937","descriptor_name":"Brassica","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D001937","descriptor_name":"Brassica","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003300","descriptor_name":"Copper","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D005721","descriptor_name":"Glutamate-Cysteine Ligase","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D008668","descriptor_name":"Metallothionein","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D013431","descriptor_name":"Sulfates","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018506","descriptor_name":"Gene Expression Regulation, Plant","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018517","descriptor_name":"Plant Roots","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019187","descriptor_name":"Cadmium Compounds","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D019287","descriptor_name":"Zinc Sulfate","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D019327","descriptor_name":"Copper Sulfate","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0014-5793(97)00132-4","doi":"https://doi.org/10.1016/s0014-5793(97)00132-4","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0014-5793(97)00132-4","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2897%2900132-4","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},{"id":"pmid:9119067","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9119067","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS letters","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0014-5793(97)00132-4","doi":"https://doi.org/10.1016/s0014-5793(97)00132-4","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0014-5793(97)00132-4","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2897%2900132-4","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2030476795.pdf","grobid_xml":"https://content.openalex.org/works/W2030476795.grobid-xml"},"referenced_works_count":25,"referenced_works":["https://openalex.org/W74320645","https://openalex.org/W947443529","https://openalex.org/W1577321104","https://openalex.org/W1974648961","https://openalex.org/W1988970390","https://openalex.org/W1991554522","https://openalex.org/W1992485982","https://openalex.org/W1992823959","https://openalex.org/W2005478615","https://openalex.org/W2008081488","https://openalex.org/W2008131835","https://openalex.org/W2012520160","https://openalex.org/W2019839309","https://openalex.org/W2024717335","https://openalex.org/W2052865318","https://openalex.org/W2074905365","https://openalex.org/W2075326198","https://openalex.org/W2090332299","https://openalex.org/W2091184424","https://openalex.org/W2123899978","https://openalex.org/W2127851451","https://openalex.org/W2136256673","https://openalex.org/W2159937829","https://openalex.org/W2170689822","https://openalex.org/W4241310852"],"related_works":["https://openalex.org/W2017950271","https://openalex.org/W1591444247","https://openalex.org/W2149234005","https://openalex.org/W2132179604","https://openalex.org/W2008832973","https://openalex.org/W4213074801","https://openalex.org/W2037740035","https://openalex.org/W4246240774","https://openalex.org/W2982241930","https://openalex.org/W2142030515"],"abstract_inverted_index":{"Glutathione":[0],"(GSH)":[1],"is":[2],"the":[3,6,27,34,117,136,166],"precursor":[4],"of":[5,50,58,65,76,83,124,138,147,158,165,173],"phytochelatins":[7],"(PC),":[8],"which":[9],"in":[10,16,30,61,68,114,131],"plants":[11],"and":[12,70,78,140,160,175],"fungi":[13],"are":[14,181],"involved":[15],"heavy":[17,35,178],"metal":[18,36,179],"sequestration.":[19],"The":[20,163],"regulatory":[21],"enzyme":[22],"gamma-glutamylcysteine":[23],"synthetase":[24],"(gamma-ECS)":[25],"catalyzes":[26],"first":[28],"step":[29],"GSH":[31,77],"biosynthesis.":[32],"For":[33],"accumulator":[37],"Brassica":[38],"juncea":[39],"L.":[40],"a":[41,62,122,128,145],"partial":[42],"gamma-ECS":[43,66,84,174],"cDNA":[44],"was":[45,86,98,112],"cloned":[46],"by":[47],"RT-PCR.":[48],"Treatment":[49],"suspension-cultured":[51],"dark":[52],"grown":[53],"seedlings":[54],"with":[55,73,168],"micromolar":[56],"concentrations":[57],"CuSO4":[59,91,103,118],"resulted":[60],"strong":[63],"increase":[64,75],"mRNA":[67,85,111],"roots":[69],"shoots,":[71],"concomitant":[72],"an":[74],"phytochelatins.":[79],"A":[80],"significant":[81],"up-regulation":[82,97],"observed":[87],"at":[88,100,121,144],"25":[89],"microM":[90,102,126,149],"(shoot":[92,104],"growth:":[93,105],"-11%),":[94],"whereas":[95],"maximum":[96],"obtained":[99],"100":[101],"-60%).":[106],"Unexpectedly,":[107],"metallothionein":[108],"2":[109],"(MT2)":[110],"decreased":[113],"response":[115],"to":[116,170],"treatments.":[119],"CdSO4":[120],"concentration":[123,146],"50":[125],"caused":[127],"72%":[129],"reduction":[130],"shoot":[132],"growth":[133,153],"without":[134],"affecting":[135],"amounts":[137],"gamma-ECS-":[139,159],"MT2":[141,161,176],"mRNAs.":[142,162],"ZnSO4":[143],"500":[148],"did":[150],"not":[151],"reduce":[152],"but":[154],"induced":[155],"transient":[156],"increases":[157],"implications":[164],"results":[167],"respect":[169],"differential":[171],"regulation":[172],"during":[177],"exposure":[180],"discussed.":[182]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}