{"id":"https://openalex.org/W2029366643","doi":"https://doi.org/10.1002/1529-0131(200012)43:12<2703::aid-anr10>3.0.co;2-y","title":"Expression of cartilage intermediate layer protein/nucleotide pyrophosphohydrolase parallels the production of extracellular inorganic pyrophosphate in response to growth factors and with aging","display_name":"Expression of cartilage intermediate layer protein/nucleotide pyrophosphohydrolase parallels the production of extracellular inorganic pyrophosphate in response to growth factors and with aging","publication_year":2000,"publication_date":"2000-12-01","ids":{"openalex":"https://openalex.org/W2029366643","doi":"https://doi.org/10.1002/1529-0131(200012)43:12<2703::aid-anr10>3.0.co;2-y","mag":"2029366643","pmid":"https://pubmed.ncbi.nlm.nih.gov/11145028"},"language":"en","primary_location":{"id":"doi:10.1002/1529-0131(200012)43:12<2703::aid-anr10>3.0.co;2-y","doi":"https://doi.org/10.1002/1529-0131(200012)43:12<2703::aid-anr10>3.0.co;2-y","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1529-0131(200012)43:12<2703::aid-anr10>3.0.co;2-y","pdf_url":null,"source":{"id":"https://openalex.org/S132787887","display_name":"Arthritis & Rheumatism","issn_l":"0004-3591","issn":["0004-3591","1529-0131"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Arthritis &amp; Rheumatism","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074120724","display_name":"Jun Hirose","orcid":"https://orcid.org/0000-0002-1752-2230","observed_orcids":["https://orcid.org/0000-0002-1752-2230"]},"institutions":[{"id":"https://openalex.org/I204308271","display_name":"Medical College of Wisconsin","ror":"https://ror.org/00qqv6244","country_code":"US","type":"education","lineage":["https://openalex.org/I204308271"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jun Hirose","raw_affiliation_strings":["Department of Medicine, Medical College of Wisconsin, Milwaukee 53226, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Medical College of Wisconsin, Milwaukee 53226, USA","institution_ids":["https://openalex.org/I204308271"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010021838","display_name":"Ikuko Masuda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204308271","display_name":"Medical College of Wisconsin","ror":"https://ror.org/00qqv6244","country_code":"US","type":"education","lineage":["https://openalex.org/I204308271"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ikuko Masuda","raw_affiliation_strings":["Medical College of Wisconsin, Milwaukee, Wisconsin, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical College of Wisconsin, Milwaukee, Wisconsin, USA","institution_ids":["https://openalex.org/I204308271"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110383846","display_name":"Lawrence M. Ryan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204308271","display_name":"Medical College of Wisconsin","ror":"https://ror.org/00qqv6244","country_code":"US","type":"education","lineage":["https://openalex.org/I204308271"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lawrence M. Ryan","raw_affiliation_strings":["Medical College of Wisconsin, Milwaukee, Wisconsin, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical College of Wisconsin, Milwaukee, Wisconsin, USA","institution_ids":["https://openalex.org/I204308271"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5074120724"],"corresponding_institution_ids":["https://openalex.org/I204308271"],"apc_list":null,"apc_paid":null,"fwci":1.8398,"has_fulltext":false,"cited_by_count":32,"citation_normalized_percentile":{"value":0.84271017,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"43","issue":"12","first_page":"2703","last_page":"2711"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10105","display_name":"Osteoarthritis Treatment and Mechanisms","score":0.79830002784729,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10105","display_name":"Osteoarthritis Treatment and Mechanisms","score":0.79830002784729,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11193","display_name":"Adenosine and Purinergic Signaling","score":0.14110000431537628,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13453","display_name":"Alkaline Phosphatase Research Studies","score":0.02290000021457672,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chondrocytes","display_name":"chondrocytes","score":0.9869999885559082},{"id":"https://openalex.org/keywords/tgf-beta1","display_name":"TGF-\u03b21","score":0.8539999723434448},{"id":"https://openalex.org/keywords/insulin-like-growth-factor-i","display_name":"insulin-like growth factor I","score":1.0},{"id":"https://openalex.org/keywords/aging","display_name":"aging","score":0.8009999990463257},{"id":"https://openalex.org/keywords/calcium-pyrophosphate-dihydrate-crystals","display_name":"calcium pyrophosphate dihydrate crystals","score":0.8740000128746033},{"id":"https://openalex.org/keywords/porcine-cartilage","display_name":"porcine cartilage","score":0.7929999828338623}],"concepts":[{"id":"https://openalex.org/C2780550940","wikidata":"https://www.wikidata.org/wiki/Q502730","display_name":"Cartilage","level":2,"score":0.5664525628089905},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5508735179901123},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.534525454044342},{"id":"https://openalex.org/C118131993","wikidata":"https://www.wikidata.org/wiki/Q423671","display_name":"Transforming growth factor","level":2,"score":0.5263432264328003},{"id":"https://openalex.org/C2776043855","wikidata":"https://www.wikidata.org/wiki/Q55506489","display_name":"Pyrophosphate","level":3,"score":0.5096840858459473},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.495602011680603},{"id":"https://openalex.org/C2781403057","wikidata":"https://www.wikidata.org/wiki/Q775480","display_name":"Chondrocyte","level":3,"score":0.49405863881111145},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.46360599994659424},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.46283435821533203},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.41743844747543335},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2860115170478821},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.21166446805000305},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.19817644357681274},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18284863233566284},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.05150225758552551}],"mesh":[{"descriptor_ui":"D000375","descriptor_name":"Aging","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002356","descriptor_name":"Cartilage","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005110","descriptor_name":"Extracellular Space","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006133","descriptor_name":"Growth Substances","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D010710","descriptor_name":"Phosphates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011755","descriptor_name":"Pyrophosphatases","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D011755","descriptor_name":"Pyrophosphatases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011755","descriptor_name":"Pyrophosphatases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011756","descriptor_name":"Diphosphates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017077","descriptor_name":"Culture Media, Conditioned","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/1529-0131(200012)43:12<2703::aid-anr10>3.0.co;2-y","doi":"https://doi.org/10.1002/1529-0131(200012)43:12<2703::aid-anr10>3.0.co;2-y","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1529-0131(200012)43:12<2703::aid-anr10>3.0.co;2-y","pdf_url":null,"source":{"id":"https://openalex.org/S132787887","display_name":"Arthritis & Rheumatism","issn_l":"0004-3591","issn":["0004-3591","1529-0131"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Arthritis &amp; Rheumatism","raw_type":"journal-article"},{"id":"pmid:11145028","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11145028","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Arthritis and rheumatism","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G313411887","display_name":null,"funder_award_id":"AR-38656","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"},{"id":"https://openalex.org/G3205141823","display_name":"Inorganic Pyrophosphate Metabolism in Arthritis","funder_award_id":"R01AR038656","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"},{"id":"https://openalex.org/G5382823443","display_name":null,"funder_award_id":"AR-44862","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"},{"id":"https://openalex.org/G6053620377","display_name":"MOLECULAR BASIS OF CALCIUM PYROPHOSPHATE DEPOSITION","funder_award_id":"R29AR044862","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"}],"funders":[{"id":"https://openalex.org/F4320337362","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":55,"referenced_works":["https://openalex.org/W3646373","https://openalex.org/W625030828","https://openalex.org/W1775749144","https://openalex.org/W1966645907","https://openalex.org/W1972006668","https://openalex.org/W1972233883","https://openalex.org/W1972590507","https://openalex.org/W1975413345","https://openalex.org/W1979684462","https://openalex.org/W1984676435","https://openalex.org/W1989500603","https://openalex.org/W1995283855","https://openalex.org/W1995764103","https://openalex.org/W1997261857","https://openalex.org/W2000413455","https://openalex.org/W2001630539","https://openalex.org/W2007146970","https://openalex.org/W2007168347","https://openalex.org/W2009239013","https://openalex.org/W2014128609","https://openalex.org/W2014733123","https://openalex.org/W2017862493","https://openalex.org/W2022383898","https://openalex.org/W2026754851","https://openalex.org/W2028502816","https://openalex.org/W2030185154","https://openalex.org/W2031673412","https://openalex.org/W2032838664","https://openalex.org/W2036137614","https://openalex.org/W2036514278","https://openalex.org/W2044192216","https://openalex.org/W2048692947","https://openalex.org/W2049672737","https://openalex.org/W2061599484","https://openalex.org/W2064362467","https://openalex.org/W2065497238","https://openalex.org/W2076968284","https://openalex.org/W2077983868","https://openalex.org/W2079645407","https://openalex.org/W2082956161","https://openalex.org/W2083313376","https://openalex.org/W2084792872","https://openalex.org/W2085809890","https://openalex.org/W2119436088","https://openalex.org/W2138270253","https://openalex.org/W2166128950","https://openalex.org/W2170085766","https://openalex.org/W2170905556","https://openalex.org/W2208689222","https://openalex.org/W2397789873","https://openalex.org/W2398871240","https://openalex.org/W2409510863","https://openalex.org/W2409967164","https://openalex.org/W2412187526","https://openalex.org/W2472955731"],"related_works":["https://openalex.org/W2097102271","https://openalex.org/W1975413345","https://openalex.org/W4253339282","https://openalex.org/W2028880433","https://openalex.org/W2077983868","https://openalex.org/W1972233883","https://openalex.org/W2461304889","https://openalex.org/W2079645407","https://openalex.org/W2050810991","https://openalex.org/W4389158607"],"abstract_inverted_index":{"OBJECTIVE:":[0],"To":[1],"evaluate":[2],"the":[3,6,123,167,235],"role":[4],"of":[5,66,87,117,174,237],"extracellular":[7],"inorganic":[8],"pyrophosphate":[9,239],"(ePPi)-generating":[10],"ectoenzyme":[11],"cartilage":[12],"intermediate":[13],"layer":[14],"protein/nucleotide":[15],"pyrophosphohydrolase":[16],"(CILP/NTPPH)":[17],"in":[18,26,111,122,146,166,179,183,207,216,223,242],"chondrocyte":[19],"PPi":[20],"elaboration,":[21,54],"we":[22],"studied":[23],"CILP/NTPPH":[24,118,141,175,192,211,220],"expression":[25,145,176,193,222],"response":[27],"to":[28],"growth":[29,47,57],"factors":[30],"during":[31],"aging.":[32],"METHODS:":[33],"Porcine":[34],"chondrocytes":[35,94,130,151,181,224],"from":[36,92,125,226],"adult":[37,93,126,148,180,208],"(3-4-year-old)":[38],"and":[39,79,100,127,136,149,156,171,194,202],"young":[40,112,128,150,168,184,231],"(2-week-old)":[41],"animals":[42,228,232],"were":[43,83,109,131,163,177],"stimulated":[44,199],"with":[45,230],"transforming":[46],"factor":[48,58],"beta1":[49],"(TGFbeta1),":[50],"which":[51,61],"enhances":[52],"ePPi":[53,63,88,195,217],"and/or":[55],"insulin-like":[56],"1":[59],"(IGF-1),":[60],"diminishes":[62],"elaboration.":[64,218],"Measurements":[65],"ePPi,":[67],"NTPPH":[68],"enzyme":[69],"activity,":[70],"Western":[71,120],"blot":[72,81],"analysis,":[73],"reverse":[74],"transcriptase-polymerase":[75],"chain":[76],"reaction":[77],"(RT-PCR),":[78],"Northern":[80,137],"analysis":[82,121,138],"performed.":[84],"RESULTS:":[85],"Elaboration":[86],"into":[89],"conditioned":[90],"media":[91,124],"was":[95,152],"significantly":[96,101],"increased":[97,132,153],"by":[98,103,119,133,154,158,200,204],"TGFbeta1":[99,155,201],"inhibited":[102],"IGF-1,":[104,159,205],"but":[105,160],"no":[106],"significant":[107,165],"differences":[108],"observed":[110],"chondrocytes.":[113,169,185],"The":[114],"protein":[115],"levels":[116,173],"porcine":[129],"TGFbeta1.":[134],"RT-PCR":[135],"showed":[139],"that":[140,191],"messenger":[142],"RNA":[143],"(mRNA)":[144],"both":[147],"decreased":[157],"these":[161],"changes":[162],"less":[164],"Basal":[170],"TGFbeta1-up-regulated":[172],"higher":[178],"than":[182],"CONCLUSION:":[186],"These":[187],"results":[188],"provide":[189],"evidence":[190],"elaboration":[196],"are":[197],"concomitantly":[198],"down-regulated":[203],"especially":[206],"chondrocytes,":[209],"implicating":[210],"as":[212],"a":[213],"functional":[214],"participant":[215],"Increased":[219],"mRNA":[221],"derived":[225],"aged":[227,243],"compared":[229],"might":[233],"promote":[234],"formation":[236],"calcium":[238],"dihydrate":[240],"crystals":[241],"cartilage.":[244]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}