{"id":"https://openalex.org/W2029314164","doi":"https://doi.org/10.1016/s0969-2126(00)00185-4","title":"A method for differentiating proteins from nucleic acids in intermediate-resolution density maps: cryo-electron microscopy defines the quaternary structure of the Escherichia coli 70S ribosome","display_name":"A method for differentiating proteins from nucleic acids in intermediate-resolution density maps: cryo-electron microscopy defines the quaternary structure of the Escherichia coli 70S ribosome","publication_year":2000,"publication_date":"2000-09-01","ids":{"openalex":"https://openalex.org/W2029314164","doi":"https://doi.org/10.1016/s0969-2126(00)00185-4","mag":"2029314164","pmid":"https://pubmed.ncbi.nlm.nih.gov/10986461"},"language":"en","primary_location":{"id":"doi:10.1016/s0969-2126(00)00185-4","doi":"https://doi.org/10.1016/s0969-2126(00)00185-4","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0969-2126(00)00185-4","pdf_url":"http://www.cell.com/article/S0969212600001854/pdf","source":{"id":"https://openalex.org/S7112016","display_name":"Structure","issn_l":"0969-2126","issn":["0969-2126","1878-4186"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Structure","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"http://www.cell.com/article/S0969212600001854/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102970624","display_name":"Christian M. T. Spahn","orcid":"https://orcid.org/0000-0002-2939-9487","observed_orcids":["https://orcid.org/0000-0002-2939-9487"]},"institutions":[{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]},{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christian MT Spahn","raw_affiliation_strings":["Howard Hughes Medical Institute, Health Research, Inc., Wadsworth Center, Empire State Plaza, Albany, NY 12201-0509, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Howard Hughes Medical Institute, Health Research, Inc., Wadsworth Center, Empire State Plaza, Albany, NY 12201-0509, USA","institution_ids":["https://openalex.org/I1339417872","https://openalex.org/I1344073410"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017567464","display_name":"Pawel A. Penczek","orcid":"https://orcid.org/0000-0002-2421-0577","observed_orcids":["https://orcid.org/0000-0002-2421-0577"]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]},{"id":"https://openalex.org/I392282","display_name":"University at Albany, State University of New York","ror":"https://ror.org/012zs8222","country_code":"US","type":"education","lineage":["https://openalex.org/I392282"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Pawel A Penczek","raw_affiliation_strings":["Department of Biomedical Sciences, State University of New York at Albany, Empire State Plaza, Albany, NY 12201-0509, USA","Wadsworth Center, Empire State Plaza, Albany, NY 12201-0509, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences, State University of New York at Albany, Empire State Plaza, Albany, NY 12201-0509, USA","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I392282"]},{"raw_affiliation_string":"Wadsworth Center, Empire State Plaza, Albany, NY 12201-0509, USA","institution_ids":["https://openalex.org/I1339417872"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108435117","display_name":"Ardean G. Leith","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ardean Leith","raw_affiliation_strings":["Wadsworth Center, Empire State Plaza, Albany, NY 12201-0509, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wadsworth Center, Empire State Plaza, Albany, NY 12201-0509, USA","institution_ids":["https://openalex.org/I1339417872"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5042892654","display_name":"Joachim Frank","orcid":"https://orcid.org/0000-0001-5449-6943","observed_orcids":["https://orcid.org/0000-0001-5449-6943","https://orcid.org/0000-0002-2442-1112"]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]},{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I392282","display_name":"University at Albany, State University of New York","ror":"https://ror.org/012zs8222","country_code":"US","type":"education","lineage":["https://openalex.org/I392282"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Joachim Frank","raw_affiliation_strings":["Department of Biomedical Sciences, State University of New York at Albany, Empire State Plaza, Albany, NY 12201-0509, USA","Howard Hughes Medical Institute, Health Research, Inc., Wadsworth Center, Empire State Plaza, Albany, NY 12201-0509, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences, State University of New York at Albany, Empire State Plaza, Albany, NY 12201-0509, USA","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I392282"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, Health Research, Inc., Wadsworth Center, Empire State Plaza, Albany, NY 12201-0509, USA","institution_ids":["https://openalex.org/I1339417872","https://openalex.org/I1344073410"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5042892654"],"corresponding_institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I1339417872","https://openalex.org/I1344073410","https://openalex.org/I392282"],"apc_list":{"value":9350,"currency":"USD","value_usd":9350},"apc_paid":null,"fwci":4.4535,"has_fulltext":false,"cited_by_count":39,"citation_normalized_percentile":{"value":0.94851995,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"8","issue":"9","first_page":"937","last_page":"948"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.7250999808311462,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.7250999808311462,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.2587999999523163,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.00860000029206276,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/70s-ribosome","display_name":"70S ribosome","score":0.8270000219345093},{"id":"https://openalex.org/keywords/quaternary-structure","display_name":"quaternary structure","score":0.8259999752044678},{"id":"https://openalex.org/keywords/ribosomal-rna","display_name":"ribosomal RNA","score":0.4860000014305115},{"id":"https://openalex.org/keywords/23s-rrna","display_name":"23S rRNA","score":0.8700000047683716},{"id":"https://openalex.org/keywords/nucleoprotein-complexes","display_name":"nucleoprotein complexes","score":0.7990000247955322}],"concepts":[{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.813236653804779},{"id":"https://openalex.org/C138268822","wikidata":"https://www.wikidata.org/wiki/Q1051925","display_name":"Resolution (logic)","level":2,"score":0.6717336773872375},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.5906856060028076},{"id":"https://openalex.org/C7927669","wikidata":"https://www.wikidata.org/wiki/Q898529","display_name":"Protein quaternary structure","level":4,"score":0.45234620571136475},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.4473584294319153},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.4460856020450592},{"id":"https://openalex.org/C20702342","wikidata":"https://www.wikidata.org/wiki/Q5190506","display_name":"Cryo-electron microscopy","level":2,"score":0.4249008297920227},{"id":"https://openalex.org/C126142528","wikidata":"https://www.wikidata.org/wiki/Q3771876","display_name":"Nucleic acid structure","level":4,"score":0.42044156789779663},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38712021708488464},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.35971397161483765},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2492212951183319},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.08617475628852844},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012269","descriptor_name":"Ribosomal Proteins","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D012269","descriptor_name":"Ribosomal Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D012329","descriptor_name":"RNA, Bacterial","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D012335","descriptor_name":"RNA, Ribosomal","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D012335","descriptor_name":"RNA, Ribosomal","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012358","descriptor_name":"RNA, Transfer, Met","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D012358","descriptor_name":"RNA, Transfer, Met","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D020285","descriptor_name":"Cryoelectron Microscopy","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D020836","descriptor_name":"Protein Structure, Quaternary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0969-2126(00)00185-4","doi":"https://doi.org/10.1016/s0969-2126(00)00185-4","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0969-2126(00)00185-4","pdf_url":"http://www.cell.com/article/S0969212600001854/pdf","source":{"id":"https://openalex.org/S7112016","display_name":"Structure","issn_l":"0969-2126","issn":["0969-2126","1878-4186"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Structure","raw_type":"journal-article"},{"id":"pmid:10986461","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10986461","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Structure (London, England : 1993)","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0969-2126(00)00185-4","doi":"https://doi.org/10.1016/s0969-2126(00)00185-4","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0969-2126(00)00185-4","pdf_url":"http://www.cell.com/article/S0969212600001854/pdf","source":{"id":"https://openalex.org/S7112016","display_name":"Structure","issn_l":"0969-2126","issn":["0969-2126","1878-4186"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Structure","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2611785866","display_name":"High Resolution in Single Particle Reconstruction","funder_award_id":"R01GM060635","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G4921166289","display_name":"Time-resolved Cryo-EM of Short-lived States in Eukaryotic Translation","funder_award_id":"R01GM055440","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G5239950330","display_name":"STRUCTURAL ANALYSIS OF MACROMOLECULAR ASSEMBLY","funder_award_id":"R37GM029169","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2029314164.pdf"},"referenced_works_count":83,"referenced_works":["https://openalex.org/W17901134","https://openalex.org/W31759787","https://openalex.org/W235677381","https://openalex.org/W653223289","https://openalex.org/W994742241","https://openalex.org/W1141204330","https://openalex.org/W1504062513","https://openalex.org/W1553162372","https://openalex.org/W1608530644","https://openalex.org/W1868347993","https://openalex.org/W1870805240","https://openalex.org/W1903945848","https://openalex.org/W1919550323","https://openalex.org/W1964652224","https://openalex.org/W1969535698","https://openalex.org/W1975455557","https://openalex.org/W1976397349","https://openalex.org/W1977287926","https://openalex.org/W1986983983","https://openalex.org/W1993131256","https://openalex.org/W1997581772","https://openalex.org/W2008025258","https://openalex.org/W2011113812","https://openalex.org/W2012879549","https://openalex.org/W2015458141","https://openalex.org/W2015729337","https://openalex.org/W2023864622","https://openalex.org/W2024442797","https://openalex.org/W2025170604","https://openalex.org/W2025641516","https://openalex.org/W2026358434","https://openalex.org/W2026817175","https://openalex.org/W2028460012","https://openalex.org/W2031006961","https://openalex.org/W2034480427","https://openalex.org/W2036912357","https://openalex.org/W2037934177","https://openalex.org/W2039353471","https://openalex.org/W2039846261","https://openalex.org/W2042049155","https://openalex.org/W2042343001","https://openalex.org/W2043540797","https://openalex.org/W2045579789","https://openalex.org/W2045612031","https://openalex.org/W2045690036","https://openalex.org/W2048357307","https://openalex.org/W2048982317","https://openalex.org/W2050711719","https://openalex.org/W2051183365","https://openalex.org/W2059792747","https://openalex.org/W2061070205","https://openalex.org/W2061146689","https://openalex.org/W2066200564","https://openalex.org/W2066267325","https://openalex.org/W2069927544","https://openalex.org/W2070052745","https://openalex.org/W2071043117","https://openalex.org/W2072093956","https://openalex.org/W2072988612","https://openalex.org/W2082167225","https://openalex.org/W2087031768","https://openalex.org/W2087173180","https://openalex.org/W2087626567","https://openalex.org/W2088745553","https://openalex.org/W2093091879","https://openalex.org/W2106329491","https://openalex.org/W2106835240","https://openalex.org/W2113761741","https://openalex.org/W2129096064","https://openalex.org/W2129210977","https://openalex.org/W2134027771","https://openalex.org/W2140071307","https://openalex.org/W2142583547","https://openalex.org/W2143712883","https://openalex.org/W2164691121","https://openalex.org/W2165724640","https://openalex.org/W2240090228","https://openalex.org/W2413477278","https://openalex.org/W2414700665","https://openalex.org/W2764845094","https://openalex.org/W6626038272","https://openalex.org/W6627452962","https://openalex.org/W6666722936"],"related_works":["https://openalex.org/W3129954825","https://openalex.org/W3208014449","https://openalex.org/W4406708102","https://openalex.org/W3036165303","https://openalex.org/W4404612151","https://openalex.org/W7202253680","https://openalex.org/W1989562772","https://openalex.org/W2777095905","https://openalex.org/W4211030178","https://openalex.org/W2614597783"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2016-06-24T00:00:00"}