{"id":"https://openalex.org/W2029287429","doi":"https://doi.org/10.1073/pnas.75.10.5025","title":"Epidermal growth factor: morphological demonstration of binding, internalization, and lysosomal association in human fibroblasts.","display_name":"Epidermal growth factor: morphological demonstration of binding, internalization, and lysosomal association in human fibroblasts.","publication_year":1978,"publication_date":"1978-10-01","ids":{"openalex":"https://openalex.org/W2029287429","doi":"https://doi.org/10.1073/pnas.75.10.5025","mag":"2029287429","pmid":"https://pubmed.ncbi.nlm.nih.gov/311005","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/336255"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.75.10.5025","doi":"https://doi.org/10.1073/pnas.75.10.5025","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.75.10.5025","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC336255","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070064147","display_name":"Phillip G\u00f6rden","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P Gorden","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108603354","display_name":"J L Carpentier","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J L Carpentier","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027994912","display_name":"Stanley Cohen","orcid":"https://orcid.org/0000-0003-3846-6028","observed_orcids":["https://orcid.org/0000-0003-3846-6028"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S Cohen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5034171652","display_name":"Lelio Orci","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L Orci","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":18.8118,"has_fulltext":false,"cited_by_count":351,"citation_normalized_percentile":{"value":0.99573864,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"75","issue":"10","first_page":"5025","last_page":"5029"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10755","display_name":"HER2/EGFR in Cancer Research","score":0.003700000001117587,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/epidermal-growth-factor","display_name":"epidermal growth factor","score":1.0},{"id":"https://openalex.org/keywords/receptor-binding","display_name":"receptor binding","score":0.6819999814033508},{"id":"https://openalex.org/keywords/receptor-internalization","display_name":"receptor internalization","score":0.7900000214576721},{"id":"https://openalex.org/keywords/lysosomes","display_name":"lysosomes","score":0.8859999775886536},{"id":"https://openalex.org/keywords/human-fibroblasts","display_name":"human fibroblasts","score":0.8870000243186951},{"id":"https://openalex.org/keywords/plasma-membrane","display_name":"plasma membrane","score":0.7599999904632568},{"id":"https://openalex.org/keywords/receptor-degradation","display_name":"receptor degradation","score":0.6759999990463257},{"id":"https://openalex.org/keywords/growth-factor-receptors","display_name":"growth factor receptors","score":0.421999990940094},{"id":"https://openalex.org/keywords/electron-microscopic-autoradiography","display_name":"electron microscopic autoradiography","score":0.6949999928474426}],"concepts":[{"id":"https://openalex.org/C139770010","wikidata":"https://www.wikidata.org/wiki/Q1339807","display_name":"Internalization","level":3,"score":0.9308808445930481},{"id":"https://openalex.org/C40017333","wikidata":"https://www.wikidata.org/wiki/Q321362","display_name":"Pinocytosis","level":4,"score":0.867551326751709},{"id":"https://openalex.org/C2776362946","wikidata":"https://www.wikidata.org/wiki/Q424374","display_name":"Epidermal growth factor","level":3,"score":0.7893233299255371},{"id":"https://openalex.org/C28005876","wikidata":"https://www.wikidata.org/wiki/Q189814","display_name":"Endocytosis","level":3,"score":0.6219013333320618},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.6094385981559753},{"id":"https://openalex.org/C197462201","wikidata":"https://www.wikidata.org/wiki/Q2476074","display_name":"Cell surface receptor","level":3,"score":0.5771490931510925},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.564727783203125},{"id":"https://openalex.org/C2778106830","wikidata":"https://www.wikidata.org/wiki/Q29548","display_name":"Cell membrane","level":3,"score":0.5494904518127441},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.5393168330192566},{"id":"https://openalex.org/C2775960820","wikidata":"https://www.wikidata.org/wiki/Q408378","display_name":"Growth factor","level":3,"score":0.5243211984634399},{"id":"https://openalex.org/C2780381497","wikidata":"https://www.wikidata.org/wiki/Q463418","display_name":"Fibroblast","level":3,"score":0.5027780532836914},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.4892670810222626},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4619489312171936},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4032723903656006},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3823580741882324},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2919467091560364},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.06263884902000427}],"mesh":[{"descriptor_ui":"D001345","descriptor_name":"Autoradiography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004815","descriptor_name":"Epidermal Growth Factor","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005347","descriptor_name":"Fibroblasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008247","descriptor_name":"Lysosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1073/pnas.75.10.5025","doi":"https://doi.org/10.1073/pnas.75.10.5025","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.75.10.5025","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:90131","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC336255","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:336255","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/336255","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:311005","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/311005","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:90131","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC336255","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W1496974366","https://openalex.org/W1501383613","https://openalex.org/W1587305227","https://openalex.org/W1970862733","https://openalex.org/W1993155805","https://openalex.org/W1998084411","https://openalex.org/W1998599114","https://openalex.org/W2047671656","https://openalex.org/W2052964328","https://openalex.org/W2062070852","https://openalex.org/W2080513099","https://openalex.org/W2085570743","https://openalex.org/W2100075764","https://openalex.org/W2164188238","https://openalex.org/W2185779328","https://openalex.org/W2415130634","https://openalex.org/W2470399128","https://openalex.org/W4239088562"],"related_works":["https://openalex.org/W2090245042","https://openalex.org/W2164188238","https://openalex.org/W2040469480","https://openalex.org/W2032422012","https://openalex.org/W2010220050","https://openalex.org/W1589389762","https://openalex.org/W1986654268","https://openalex.org/W7099795732","https://openalex.org/W1958612658","https://openalex.org/W1186290499"],"abstract_inverted_index":{"Using":[0],"a":[1,40],"quantitative":[2],"electron":[3],"microscopic":[4],"autoradiographic":[5,56],"technique,":[6],"we":[7],"have":[8,67],"localized":[9],"the":[10,21,25,37,55,61,73,87,109,120,145],"initial":[11,29],"binding":[12,133],"step":[13],"of":[14,24,54,102,108,119,134,144],"125I-labeled":[15],"epidermal":[16],"growth":[17],"factor":[18],"(125I-EGF)":[19],"to":[20,49,60,80,105],"plasma":[22,62],"membrane":[23,63,121],"human":[26],"fibroblast.":[27],"After":[28],"binding,":[30],"labeled":[31],"EGF":[32,135],"is":[33,136],"internalized":[34,74],"progressively":[35],"by":[36,138],"cell":[38,150],"in":[39],"time-":[41],"and":[42,64,129,141,147],"temperature-dependent":[43],"fashion;":[44],"when":[45],"cell-associated":[46],"radioactivity":[47],"comes":[48],"steady":[50],"state,":[51],"approximately":[52,65],"1/3":[53],"grains":[57,75,103],"are":[58,76],"related":[59,79],"2/3":[66],"been":[68],"internalized.":[69],"Under":[70],"these":[71],"conditions":[72],"almost":[77],"exclusively":[78],"lysosomal":[81],"structures.":[82],"When":[83],"125I-EGF":[84],"associates":[85],"with":[86],"cells":[88],"for":[89],"2":[90,96],"hr":[91],"at":[92,98],"4":[93],"degrees":[94],"or":[95],"min":[97],"37":[99],"degrees,":[100],"34%":[101],"localize":[104],"coated":[106,112],"regions":[107,113],"membrane.":[110],"These":[111,123],"make":[114],"up":[115],"less":[116],"than":[117],"2%":[118],"surface.":[122],"data":[124],"directly":[125],"confirm":[126],"kinetic":[127],"studies":[128],"suggest":[130],"that":[131],"saturable":[132],"followed":[137],"adsorptive":[139],"pinocytosis":[140],"cellular":[142],"degradation":[143],"ligand":[146],"possibly":[148],"its":[149],"surface":[151],"receptor.":[152]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}