{"id":"https://openalex.org/W2028811698","doi":"https://doi.org/10.1097/01.wcb.0000127661.07591.de","title":"Fluorometric Determination of Glucose Utilization in Neurons in Vitro and in Vivo","display_name":"Fluorometric Determination of Glucose Utilization in Neurons in Vitro and in Vivo","publication_year":2004,"publication_date":"2004-08-25","ids":{"openalex":"https://openalex.org/W2028811698","doi":"https://doi.org/10.1097/01.wcb.0000127661.07591.de","mag":"2028811698","pmid":"https://pubmed.ncbi.nlm.nih.gov/15356420"},"language":"en","primary_location":{"id":"doi:10.1097/01.wcb.0000127661.07591.de","doi":"https://doi.org/10.1097/01.wcb.0000127661.07591.de","is_oa":false,"landing_page_url":"https://doi.org/10.1097/01.wcb.0000127661.07591.de","pdf_url":null,"source":{"id":"https://openalex.org/S89339265","display_name":"Journal of Cerebral Blood Flow & Metabolism","issn_l":"0271-678X","issn":["0271-678X","1559-7016"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cerebral Blood Flow & Metabolism","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070424908","display_name":"Yoshiaki Itoh","orcid":"https://orcid.org/0000-0001-5731-798X","observed_orcids":["https://orcid.org/0000-0001-5731-798X"]},"institutions":[{"id":"https://openalex.org/I203951103","display_name":"Keio University","ror":"https://ror.org/02kn6nx58","country_code":"JP","type":"education","lineage":["https://openalex.org/I203951103"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Yoshiaki Itoh","raw_affiliation_strings":["From the Department of Neurology, Keio University School of Medicine, Shinjuku, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Neurology, Keio University School of Medicine, Shinjuku, Tokyo, Japan","institution_ids":["https://openalex.org/I203951103"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101490820","display_name":"Takato Abe","orcid":"https://orcid.org/0000-0002-4185-5254","observed_orcids":["https://orcid.org/0000-0002-4185-5254"]},"institutions":[{"id":"https://openalex.org/I203951103","display_name":"Keio University","ror":"https://ror.org/02kn6nx58","country_code":"JP","type":"education","lineage":["https://openalex.org/I203951103"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takato Abe","raw_affiliation_strings":["From the Department of Neurology, Keio University School of Medicine, Shinjuku, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Neurology, Keio University School of Medicine, Shinjuku, Tokyo, Japan","institution_ids":["https://openalex.org/I203951103"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017354494","display_name":"Rie Takaoka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I203951103","display_name":"Keio University","ror":"https://ror.org/02kn6nx58","country_code":"JP","type":"education","lineage":["https://openalex.org/I203951103"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Rie Takaoka","raw_affiliation_strings":["From the Department of Neurology, Keio University School of Medicine, Shinjuku, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Neurology, Keio University School of Medicine, Shinjuku, Tokyo, Japan","institution_ids":["https://openalex.org/I203951103"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111606434","display_name":"Norio Tanahashi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I203951103","display_name":"Keio University","ror":"https://ror.org/02kn6nx58","country_code":"JP","type":"education","lineage":["https://openalex.org/I203951103"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Norio Tanahashi","raw_affiliation_strings":["From the Department of Neurology, Keio University School of Medicine, Shinjuku, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Neurology, Keio University School of Medicine, Shinjuku, Tokyo, Japan","institution_ids":["https://openalex.org/I203951103"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5070424908"],"corresponding_institution_ids":["https://openalex.org/I203951103"],"apc_list":{"value":4200,"currency":"USD","value_usd":4200},"apc_paid":null,"fwci":1.5224,"has_fulltext":false,"cited_by_count":72,"citation_normalized_percentile":{"value":0.78911384,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"24","issue":"9","first_page":"993","last_page":"1003"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.8618000149726868,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.8618000149726868,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11872","display_name":"Barrier Structure and Function Studies","score":0.010200000368058681,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.009200000204145908,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glucose-utilization","display_name":"glucose utilization","score":0.7200000286102295},{"id":"https://openalex.org/keywords/neurons","display_name":"neurons","score":0.8289999961853027},{"id":"https://openalex.org/keywords/astroglia","display_name":"astroglia","score":0.7649999856948853},{"id":"https://openalex.org/keywords/2-nbdg","display_name":"2-NBDG","score":0.9610000252723694},{"id":"https://openalex.org/keywords/2-deoxy-d-glucose","display_name":"2-deoxyglucose","score":0.8259999752044678},{"id":"https://openalex.org/keywords/glucose-metabolism","display_name":"glucose metabolism","score":0.6700000166893005},{"id":"https://openalex.org/keywords/brain-energy-metabolism","display_name":"brain energy metabolism","score":0.7739999890327454},{"id":"https://openalex.org/keywords/lactate","display_name":"lactate","score":0.5809999704360962},{"id":"https://openalex.org/keywords/hippocampal-neurons","display_name":"hippocampal neurons","score":0.8450000286102295},{"id":"https://openalex.org/keywords/purkinje-cells","display_name":"Purkinje cells","score":0.9229999780654907}],"concepts":[{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.8309996724128723},{"id":"https://openalex.org/C148762608","wikidata":"https://www.wikidata.org/wiki/Q3748185","display_name":"Hippocampal formation","level":2,"score":0.6214881539344788},{"id":"https://openalex.org/C17093226","wikidata":"https://www.wikidata.org/wiki/Q2734081","display_name":"Carbohydrate metabolism","level":2,"score":0.6161728501319885},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5713027715682983},{"id":"https://openalex.org/C2780909259","wikidata":"https://www.wikidata.org/wiki/Q5260067","display_name":"Deoxyglucose","level":2,"score":0.5384860038757324},{"id":"https://openalex.org/C161573976","wikidata":"https://www.wikidata.org/wiki/Q420277","display_name":"Glucose transporter","level":3,"score":0.48570045828819275},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.47158992290496826},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4712280035018921},{"id":"https://openalex.org/C2777866211","wikidata":"https://www.wikidata.org/wiki/Q5572349","display_name":"Glucose uptake","level":3,"score":0.4651758372783661},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.45534002780914307},{"id":"https://openalex.org/C2982719155","wikidata":"https://www.wikidata.org/wiki/Q25584060","display_name":"Energy source","level":3,"score":0.4311135411262512},{"id":"https://openalex.org/C43376680","wikidata":"https://www.wikidata.org/wiki/Q6580786","display_name":"Bolus (digestion)","level":2,"score":0.42866918444633484},{"id":"https://openalex.org/C29694410","wikidata":"https://www.wikidata.org/wiki/Q7540236","display_name":"Slice preparation","level":3,"score":0.41558241844177246},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.39561158418655396},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3920760750770569},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3205292224884033},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.30879324674606323},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.2665852904319763},{"id":"https://openalex.org/C2779306644","wikidata":"https://www.wikidata.org/wiki/Q2002370","display_name":"Insulin","level":2,"score":0.2253515124320984},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.14861077070236206},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0},{"id":"https://openalex.org/C188573790","wikidata":"https://www.wikidata.org/wiki/Q12705","display_name":"Renewable energy","level":2,"score":0.0},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001253","descriptor_name":"Astrocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001921","descriptor_name":"Brain","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005470","descriptor_name":"Fluorometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005944","descriptor_name":"Glucosamine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D005944","descriptor_name":"Glucosamine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009327","descriptor_name":"4-Chloro-7-nitrobenzofurazan","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D009327","descriptor_name":"4-Chloro-7-nitrobenzofurazan","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009474","descriptor_name":"Neurons","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017207","descriptor_name":"Rats, Sprague-Dawley","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1097/01.wcb.0000127661.07591.de","doi":"https://doi.org/10.1097/01.wcb.0000127661.07591.de","is_oa":false,"landing_page_url":"https://doi.org/10.1097/01.wcb.0000127661.07591.de","pdf_url":null,"source":{"id":"https://openalex.org/S89339265","display_name":"Journal of Cerebral Blood Flow & Metabolism","issn_l":"0271-678X","issn":["0271-678X","1559-7016"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cerebral Blood Flow & Metabolism","raw_type":"journal-article"},{"id":"pmid:15356420","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15356420","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8048999905586243,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337346","display_name":"National Institute of Mental Health","ror":"https://ror.org/04xeg9z08"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":26,"referenced_works":["https://openalex.org/W43284170","https://openalex.org/W221359381","https://openalex.org/W1536070820","https://openalex.org/W1567517382","https://openalex.org/W1964143634","https://openalex.org/W1975222337","https://openalex.org/W1984476260","https://openalex.org/W1988147369","https://openalex.org/W2024648928","https://openalex.org/W2037575256","https://openalex.org/W2043370439","https://openalex.org/W2053268711","https://openalex.org/W2057065995","https://openalex.org/W2057539606","https://openalex.org/W2068083749","https://openalex.org/W2071122107","https://openalex.org/W2075348611","https://openalex.org/W2090306035","https://openalex.org/W2108207203","https://openalex.org/W2119476349","https://openalex.org/W2123716968","https://openalex.org/W2137692368","https://openalex.org/W2151818004","https://openalex.org/W2153883114","https://openalex.org/W2409153565","https://openalex.org/W2414425146"],"related_works":["https://openalex.org/W2055338978","https://openalex.org/W2070629895","https://openalex.org/W7097683372","https://openalex.org/W2036267962","https://openalex.org/W2113429577","https://openalex.org/W2009966509","https://openalex.org/W2053405269","https://openalex.org/W2116325992","https://openalex.org/W759039073","https://openalex.org/W2111261869"],"abstract_inverted_index":{"Glucose":[0],"is":[1,59],"the":[2,6,26,39,42,45,133],"major":[3],"energy":[4,24,126,180],"source":[5,127],"adult":[7],"brain":[8,40,119,157],"utilizes":[9],"under":[10],"physiologic":[11],"conditions.":[12],"Recent":[13],"findings,":[14],"however,":[15],"have":[16,49],"suggested":[17],"that":[18,101,115,160,172],"neurons":[19,74,81,111,166,173],"obtain":[20],"most":[21],"of":[22,28,35,56,118,148,156],"their":[23,125],"from":[25,32,41],"oxidation":[27],"extracellular":[29],"lactate":[30],"derived":[31],"astroglial":[33],"metabolism":[34,72],"glucose":[36,64,71,123,176],"transported":[37],"into":[38],"blood.":[43],"In":[44],"present":[46],"studies":[47],"we":[48],"used":[50,61],"2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose":[51],"(2-NBDG),":[52],"a":[53,145],"fluorescent":[54],"analogue":[55],"2-deoxyglucose,":[57],"which":[58],"often":[60],"to":[62,69,90],"trace":[63],"utilization":[65],"in":[66,73,75,78,109,128,137,164,177],"neural":[67],"tissues,":[68],"examine":[70],"vitro":[76],"and":[77,82,93,112,150,167],"vivo.":[79],"Cultured":[80],"astroglia":[83],"were":[84,135,141],"incubated":[85],"with":[86,106,144],"2-NBDG":[87,95,149,162],"for":[88],"up":[89],"15":[91],"minutes,":[92],"nonmetabolized":[94],"was":[96],"washed":[97],"out.":[98],"We":[99],"found":[100],"fluorescence":[102],"intensity":[103],"increased":[104],"linearly":[105],"incubation":[107],"time":[108],"both":[110,116],"astroglia,":[113],"indicating":[114,171],"types":[117],"cells":[120],"could":[121],"utilize":[122,175],"as":[124,179],"vitro.":[129],"To":[130],"determine":[131],"if":[132],"same":[134],"true":[136],"vivo,":[138],"Sprague-Dawley":[139],"rats":[140],"injected":[142],"intravenously":[143],"pulse":[146],"bolus":[147],"decapitated":[151],"45":[152],"minutes":[153],"later.":[154],"Examination":[155],"sections":[158],"demonstrated":[159],"phosphorylated":[161],"accumulated":[163],"hippocampal":[165],"cerebellar":[168],"Purkinje":[169],"cells,":[170],"can":[174],"vivo":[178],"source.":[181]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":6}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}