{"id":"https://openalex.org/W2028082957","doi":"https://doi.org/10.1016/0006-291x(66)90447-5","title":"A membrane-filter technique for the detection of complementary DNA","display_name":"A membrane-filter technique for the detection of complementary DNA","publication_year":1966,"publication_date":"1966-06-01","ids":{"openalex":"https://openalex.org/W2028082957","doi":"https://doi.org/10.1016/0006-291x(66)90447-5","mag":"2028082957","pmid":"https://pubmed.ncbi.nlm.nih.gov/5963888"},"language":"en","primary_location":{"id":"doi:10.1016/0006-291x(66)90447-5","doi":"https://doi.org/10.1016/0006-291x(66)90447-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0006-291x(66)90447-5","pdf_url":null,"source":{"id":"https://openalex.org/S151589183","display_name":"Biochemical and Biophysical Research Communications","issn_l":"0006-291X","issn":["0006-291X","1090-2104"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical and Biophysical Research Communications","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027560531","display_name":"David T. Denhardt","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"David T. Denhardt","raw_affiliation_strings":["The Biological Laboratories Harvard University Cambridge, Massachusetts 02138 USA","The Biological Laboratories, Harvard University Cambridge, Massachusetts 02138 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Biological Laboratories Harvard University Cambridge, Massachusetts 02138 USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"The Biological Laboratories, Harvard University Cambridge, Massachusetts 02138 USA","institution_ids":["https://openalex.org/I136199984"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5027560531"],"corresponding_institution_ids":["https://openalex.org/I136199984"],"apc_list":{"value":3110,"currency":"USD","value_usd":3110},"apc_paid":null,"fwci":20.0086,"has_fulltext":false,"cited_by_count":3885,"citation_normalized_percentile":{"value":0.99844217,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"23","issue":"5","first_page":"641","last_page":"646"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8086000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8086000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.12720000743865967,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.02810000069439411,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/membrane-filtration","display_name":"membrane filtration","score":0.718999981880188},{"id":"https://openalex.org/keywords/southern-blot","display_name":"Southern blot","score":0.6660000085830688},{"id":"https://openalex.org/keywords/radioactive-probes","display_name":"radioactive probes","score":0.6859999895095825},{"id":"https://openalex.org/keywords/gene-targeting","display_name":"gene targeting","score":0.6549999713897705},{"id":"https://openalex.org/keywords/embryonic-stem-cells","display_name":"embryonic stem cells","score":0.7770000100135803},{"id":"https://openalex.org/keywords/homologous-recombination","display_name":"homologous recombination","score":0.9079999923706055},{"id":"https://openalex.org/keywords/mouse-model","display_name":"mouse model","score":0.6050000190734863}],"concepts":[{"id":"https://openalex.org/C133307689","wikidata":"https://www.wikidata.org/wiki/Q48749","display_name":"Southern blot","level":3,"score":0.7520561218261719},{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.6175020337104797},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5439193248748779},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5402526259422302},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5302203297615051},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5014321804046631},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.41366660594940186},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.40774744749069214},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.39776843786239624}],"mesh":[{"descriptor_ui":"D003090","descriptor_name":"Coliphages","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003090","descriptor_name":"Coliphages","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003090","descriptor_name":"Coliphages","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D005374","descriptor_name":"Filtration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005374","descriptor_name":"Filtration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005374","descriptor_name":"Filtration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011134","descriptor_name":"Polysaccharides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011134","descriptor_name":"Polysaccharides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011134","descriptor_name":"Polysaccharides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011205","descriptor_name":"Povidone","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011205","descriptor_name":"Povidone","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011205","descriptor_name":"Povidone","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012710","descriptor_name":"Serum Albumin, Bovine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012710","descriptor_name":"Serum Albumin, Bovine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012710","descriptor_name":"Serum Albumin, Bovine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0006-291x(66)90447-5","doi":"https://doi.org/10.1016/0006-291x(66)90447-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0006-291x(66)90447-5","pdf_url":null,"source":{"id":"https://openalex.org/S151589183","display_name":"Biochemical and Biophysical Research Communications","issn_l":"0006-291X","issn":["0006-291X","1090-2104"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical and Biophysical Research Communications","raw_type":"journal-article"},{"id":"pmid:5963888","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/5963888","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical and biophysical research communications","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":3,"referenced_works":["https://openalex.org/W272949472","https://openalex.org/W1975740516","https://openalex.org/W2088974713"],"related_works":["https://openalex.org/W205422438","https://openalex.org/W2086525401","https://openalex.org/W2006735166","https://openalex.org/W2079327011","https://openalex.org/W2053325634","https://openalex.org/W193851735","https://openalex.org/W3178279768","https://openalex.org/W160849336","https://openalex.org/W2147164622","https://openalex.org/W2773861473"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":11},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":4}],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2025-10-10T00:00:00"}