{"id":"https://openalex.org/W2025628137","doi":"https://doi.org/10.1577/1548-8667(2001)013<0163:eaooad>2.0.co;2","title":"Evaluation and Optimization of a DNA Transfer System forEdwardsiella ictaluri","display_name":"Evaluation and Optimization of a DNA Transfer System forEdwardsiella ictaluri","publication_year":2001,"publication_date":"2001-06-01","ids":{"openalex":"https://openalex.org/W2025628137","doi":"https://doi.org/10.1577/1548-8667(2001)013<0163:eaooad>2.0.co;2","mag":"2025628137"},"language":"en","primary_location":{"id":"doi:10.1577/1548-8667(2001)013<0163:eaooad>2.0.co;2","doi":"https://doi.org/10.1577/1548-8667(2001)013<0163:eaooad>2.0.co;2","is_oa":false,"landing_page_url":"https://doi.org/10.1577/1548-8667(2001)013<0163:eaooad>2.0.co;2","pdf_url":null,"source":{"id":"https://openalex.org/S194317145","display_name":"Journal of Aquatic Animal Health","issn_l":"0899-7659","issn":["0899-7659","1548-8667"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Aquatic Animal Health","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5013714705","display_name":"Kirk J. Maurer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kirk J. Maurer","raw_affiliation_strings":["Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, 70803 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, 70803 USA","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075574028","display_name":"Mark L. Lawrence","orcid":"https://orcid.org/0000-0003-4570-5592","observed_orcids":["https://orcid.org/0000-0003-4570-5592"]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]},{"id":"https://openalex.org/I99041443","display_name":"Mississippi State University","ror":"https://ror.org/0432jq872","country_code":"US","type":"education","lineage":["https://openalex.org/I4210141039","https://openalex.org/I99041443"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark L. Lawrence","raw_affiliation_strings":["Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, 70803 USA","Present address: College of Veterinary Medicine, Mississippi State, Mississippi 39762, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, 70803 USA","institution_ids":["https://openalex.org/I121820613"]},{"raw_affiliation_string":"Present address: College of Veterinary Medicine, Mississippi State, Mississippi 39762, USA","institution_ids":["https://openalex.org/I99041443"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111881010","display_name":"Denise H. Fernandez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]},{"id":"https://openalex.org/I134386540","display_name":"Louisiana State University Agricultural Center","ror":"https://ror.org/01b8rza40","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613","https://openalex.org/I134386540"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Denise H. Fernandez","raw_affiliation_strings":["Department of Veterinary Science, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, 70803 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Science, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, 70803 USA","institution_ids":["https://openalex.org/I121820613","https://openalex.org/I134386540"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111707255","display_name":"Ronald L. Thune","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]},{"id":"https://openalex.org/I134386540","display_name":"Louisiana State University Agricultural Center","ror":"https://ror.org/01b8rza40","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613","https://openalex.org/I134386540"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ronald L. Thune","raw_affiliation_strings":["Departments of Veterinary Science, Louisiana State University Agricultural Center, and Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, 70802 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Veterinary Science, Louisiana State University Agricultural Center, and Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, 70802 USA","institution_ids":["https://openalex.org/I121820613","https://openalex.org/I134386540"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5111707255"],"corresponding_institution_ids":["https://openalex.org/I121820613","https://openalex.org/I134386540"],"apc_list":{"value":3500,"currency":"USD","value_usd":3500},"apc_paid":null,"fwci":0.5414,"has_fulltext":false,"cited_by_count":17,"citation_normalized_percentile":{"value":0.65800325,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"13","issue":"2","first_page":"163","last_page":"167"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9150000214576721,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9150000214576721,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10506","display_name":"Aquaculture disease management and microbiota","score":0.07779999822378159,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/edwardsiella-ictaluri","display_name":"Edwardsiella ictaluri","score":0.9980000257492065},{"id":"https://openalex.org/keywords/dna-transfer","display_name":"DNA transfer","score":0.847000002861023},{"id":"https://openalex.org/keywords/conjugation","display_name":"conjugation","score":0.6169999837875366},{"id":"https://openalex.org/keywords/suicide-vector","display_name":"suicide vector","score":0.9810000061988831},{"id":"https://openalex.org/keywords/isogenic-mutants","display_name":"isogenic mutants","score":0.7170000076293945},{"id":"https://openalex.org/keywords/fish-pathogens","display_name":"fish pathogens","score":0.7559999823570251},{"id":"https://openalex.org/keywords/plasmid-integration","display_name":"plasmid integration","score":0.8109999895095825},{"id":"https://openalex.org/keywords/ampicillin-resistance","display_name":"ampicillin resistance","score":0.8259999752044678},{"id":"https://openalex.org/keywords/allelic-exchange","display_name":"allelic exchange","score":0.7480000257492065},{"id":"https://openalex.org/keywords/complementation","display_name":"complementation","score":0.7590000033378601}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8433138132095337},{"id":"https://openalex.org/C2911177997","wikidata":"https://www.wikidata.org/wiki/Q5346148","display_name":"Edwardsiella ictaluri","level":5,"score":0.7106322050094604},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6689776182174683},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.6064722537994385},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5433578491210938},{"id":"https://openalex.org/C4918238","wikidata":"https://www.wikidata.org/wiki/Q121438","display_name":"Transposable element","level":4,"score":0.5376509428024292},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5349188446998596},{"id":"https://openalex.org/C2778022156","wikidata":"https://www.wikidata.org/wiki/Q576145","display_name":"Strain (injury)","level":2,"score":0.5191166400909424},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5066102743148804},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.47707995772361755},{"id":"https://openalex.org/C28693550","wikidata":"https://www.wikidata.org/wiki/Q7835335","display_name":"Transposon mutagenesis","level":5,"score":0.47499504685401917},{"id":"https://openalex.org/C188082640","wikidata":"https://www.wikidata.org/wiki/Q1780899","display_name":"Complementation","level":4,"score":0.4489273428916931},{"id":"https://openalex.org/C186852380","wikidata":"https://www.wikidata.org/wiki/Q1142521","display_name":"Electroporation","level":3,"score":0.43366992473602295},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.35896334052085876},{"id":"https://openalex.org/C2778189562","wikidata":"https://www.wikidata.org/wiki/Q59576","display_name":"Catfish","level":3,"score":0.18544507026672363},{"id":"https://openalex.org/C2909208804","wikidata":"https://www.wikidata.org/wiki/Q127282","display_name":"Fish <Actinopterygii>","level":2,"score":0.18176758289337158},{"id":"https://openalex.org/C2778949639","wikidata":"https://www.wikidata.org/wiki/Q1711508","display_name":"Ictalurus","level":4,"score":0.08842888474464417},{"id":"https://openalex.org/C505870484","wikidata":"https://www.wikidata.org/wiki/Q180538","display_name":"Fishery","level":1,"score":0.0},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1577/1548-8667(2001)013<0163:eaooad>2.0.co;2","doi":"https://doi.org/10.1577/1548-8667(2001)013<0163:eaooad>2.0.co;2","is_oa":false,"landing_page_url":"https://doi.org/10.1577/1548-8667(2001)013<0163:eaooad>2.0.co;2","pdf_url":null,"source":{"id":"https://openalex.org/S194317145","display_name":"Journal of Aquatic Animal Health","issn_l":"0899-7659","issn":["0899-7659","1548-8667"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Aquatic Animal Health","raw_type":"journal-article"},{"id":"pmh:oai:digitalcommons.lsu.edu:animalsciences_pubs-2429","doi":null,"is_oa":false,"landing_page_url":"https://digitalcommons.lsu.edu/animalsciences_pubs/1430","pdf_url":null,"source":{"id":"https://openalex.org/S4210169993","display_name":"Civil War Book Review","issn_l":"1528-6592","issn":["1528-6592"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315936","host_organization_name":"Louisiana State University","host_organization_lineage":["https://openalex.org/P4310315936"],"host_organization_lineage_names":["Louisiana State University"],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Faculty Publications","raw_type":"text"},{"id":"pmh:oai:repository.lsu.edu:animalsciences_pubs-2429","doi":null,"is_oa":false,"landing_page_url":"https://repository.lsu.edu/animalsciences_pubs/1430","pdf_url":null,"source":{"id":"https://openalex.org/S4210169993","display_name":"Civil War Book Review","issn_l":"1528-6592","issn":["1528-6592"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315936","host_organization_name":"Louisiana State University","host_organization_lineage":["https://openalex.org/P4310315936"],"host_organization_lineage_names":["Louisiana State University"],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Faculty Publications","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":8,"referenced_works":["https://openalex.org/W193267154","https://openalex.org/W208046272","https://openalex.org/W1787690277","https://openalex.org/W1824614244","https://openalex.org/W2046524869","https://openalex.org/W2051455093","https://openalex.org/W2168997585","https://openalex.org/W4241815492"],"related_works":["https://openalex.org/W2020204454","https://openalex.org/W2481698932","https://openalex.org/W2938913195","https://openalex.org/W2747279822","https://openalex.org/W2258076924","https://openalex.org/W2556959014","https://openalex.org/W2163670830","https://openalex.org/W2151536105","https://openalex.org/W1978544314","https://openalex.org/W2323159023"],"abstract_inverted_index":{"To":[0],"develop":[1],"DNA":[2],"transfer":[3,45,219],"and":[4,21,46,67,96,129,146,206,216],"mutagenesis":[5],"procedures":[6],"for":[7,12,89,124,211,217],"Edwardsiella":[8],"ictaluri,":[9],"optimal":[10,51,121],"conditions":[11],"exchange":[13],"between":[14],"a":[15,22,35,40,72,101,140,167],"recipient":[16,97,131],"strain":[17,24],"of":[18,25,34,55,57,74,84,104,110,117,158,170,193],"E.":[19,58,127,132,198],"ictaluri":[20,59,199],"donor":[23,61,95,126],"Escherichia":[26],"coli":[27,128],"were":[28,50,80,162],"experimentally":[29],"determined":[30],"by":[31,178,220],"the":[32,53,82,118,125,130,159],"use":[33,212],"mobilizable":[36],"transposon":[37],"carried":[38],"on":[39],"suicide":[41],"vector.":[42],"In":[43],"general,":[44],"subsequent":[47],"mutant":[48,111,135],"production":[49,112,136,151],"when":[52],"ratio":[54,73,103],"cells":[56,62],"to":[60,65,71,149,181,188],"was":[63,68,113,152],"4:1":[64],"10:1":[66],"satisfactory":[69],"out":[70],"45:1.":[75],"Initial":[76],"cell":[77,98],"concentrations":[78],"also":[79,208],"important;":[81],"number":[83],"mutants":[85,160,196],"produced":[86,161],"approximately":[87],"doubled":[88],"each":[90],"log":[91],"increase":[92],"in":[93,120,163,197,213],"initial":[94],"numbers":[99,192],"at":[100,138,144,154],"recipient-to-donor":[102],"31:1.":[105],"The":[106,156],"most":[107],"important":[108],"determinant":[109],"temperature,":[114],"primarily":[115],"because":[116],"difference":[119],"growth":[122],"temperatures":[123],"ictaluri.":[133],"Optimal":[134],"occurred":[137,143],"24\u00b0C,":[139],"considerable":[141],"reduction":[142],"28\u00b0C,":[145],"very":[147],"poor":[148],"zero":[150],"seen":[153],"33\u00b0C.":[155],"majority":[157],"single":[164],"loci,":[165],"with":[166],"low":[168],"frequency":[169],"plasmid":[171],"integration":[172],"events":[173],"that":[174],"could":[175],"be":[176,186,209],"detected":[177],"establishing":[179],"resistance":[180],"ampicillin.":[182],"This":[183],"system":[184],"can":[185,207],"used":[187],"efficiently":[189],"produce":[190],"large":[191],"stable,":[194],"isogenic":[195],"as":[200,202],"well":[201],"other":[203],"fish":[204],"pathogens":[205],"adapted":[210],"complementation":[214],"studies":[215],"gene":[218],"allelic":[221],"exchange.":[222]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-08T09:06:37.294493","created_date":"2016-06-24T00:00:00"}