{"id":"https://openalex.org/W2025577628","doi":"https://doi.org/10.1139/b88-228","title":"Improving microspore culture as a rapeseed breeding tool: the use of auxins and cytokinins in an induction medium","display_name":"Improving microspore culture as a rapeseed breeding tool: the use of auxins and cytokinins in an induction medium","publication_year":1988,"publication_date":"1988-08-01","ids":{"openalex":"https://openalex.org/W2025577628","doi":"https://doi.org/10.1139/b88-228","mag":"2025577628"},"language":"en","primary_location":{"id":"doi:10.1139/b88-228","doi":"https://doi.org/10.1139/b88-228","is_oa":false,"landing_page_url":"https://doi.org/10.1139/b88-228","pdf_url":null,"source":{"id":"https://openalex.org/S4210188060","display_name":"Canadian Journal of Botany","issn_l":"0008-4026","issn":["0008-4026","1480-3305"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310312929","host_organization_name":"Canadian Science Publishing","host_organization_lineage":["https://openalex.org/P4310312929"],"host_organization_lineage_names":["Canadian Science Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Canadian Journal of Botany","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078289942","display_name":"David G. Charne","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D. G. Charne","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5110153856","display_name":"Wallace D. Beversdorf","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"W. D. Beversdorf","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.7079,"has_fulltext":false,"cited_by_count":39,"citation_normalized_percentile":{"value":0.94675786,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"66","issue":"8","first_page":"1671","last_page":"1675"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12566","display_name":"Nitrogen and Sulfur Effects on Brassica","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1102","display_name":"Agronomy and Crop Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microspore-embryogenesis","display_name":"microspore embryogenesis","score":0.9380000233650208},{"id":"https://openalex.org/keywords/brassica-napus","display_name":"Brassica napus","score":0.9169999957084656},{"id":"https://openalex.org/keywords/rapeseed-breeding","display_name":"rapeseed breeding","score":0.9449999928474426},{"id":"https://openalex.org/keywords/naphthaleneacetic-acid","display_name":"naphthaleneacetic acid","score":0.9480000138282776},{"id":"https://openalex.org/keywords/6-benzyladenine","display_name":"6-benzyladenine","score":0.9710000157356262},{"id":"https://openalex.org/keywords/auxin","display_name":"auxin","score":0.8510000109672546},{"id":"https://openalex.org/keywords/cytokinins","display_name":"cytokinins","score":0.9980000257492065},{"id":"https://openalex.org/keywords/embryo-yield","display_name":"embryo yield","score":0.8519999980926514},{"id":"https://openalex.org/keywords/cotyledonary-embryos","display_name":"cotyledonary embryos","score":0.9449999928474426},{"id":"https://openalex.org/keywords/f1-hybrids","display_name":"F1 hybrids","score":0.4880000054836273}],"concepts":[{"id":"https://openalex.org/C2779726014","wikidata":"https://www.wikidata.org/wiki/Q177932","display_name":"Rapeseed","level":2,"score":0.8808808922767639},{"id":"https://openalex.org/C121807389","wikidata":"https://www.wikidata.org/wiki/Q2452297","display_name":"Microspore","level":4,"score":0.8768833875656128},{"id":"https://openalex.org/C207778908","wikidata":"https://www.wikidata.org/wiki/Q190022","display_name":"Auxin","level":3,"score":0.8602496385574341},{"id":"https://openalex.org/C2780369116","wikidata":"https://www.wikidata.org/wiki/Q422032","display_name":"Cytokinin","level":4,"score":0.826179563999176},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.7484521269798279},{"id":"https://openalex.org/C2776747608","wikidata":"https://www.wikidata.org/wiki/Q58677","display_name":"Brassica","level":2,"score":0.7066478729248047},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6177932620048523},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.5915285348892212},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.506625235080719},{"id":"https://openalex.org/C21410773","wikidata":"https://www.wikidata.org/wiki/Q220869","display_name":"Shoot","level":2,"score":0.4790574014186859},{"id":"https://openalex.org/C48189365","wikidata":"https://www.wikidata.org/wiki/Q42621","display_name":"Hybrid","level":2,"score":0.4207206964492798},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.4113644063472748},{"id":"https://openalex.org/C29172406","wikidata":"https://www.wikidata.org/wiki/Q103129","display_name":"Stamen","level":3,"score":0.15351754426956177},{"id":"https://openalex.org/C2780618852","wikidata":"https://www.wikidata.org/wiki/Q79932","display_name":"Pollen","level":2,"score":0.11324337124824524},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.10362178087234497},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.08615085482597351},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1139/b88-228","doi":"https://doi.org/10.1139/b88-228","is_oa":false,"landing_page_url":"https://doi.org/10.1139/b88-228","pdf_url":null,"source":{"id":"https://openalex.org/S4210188060","display_name":"Canadian Journal of Botany","issn_l":"0008-4026","issn":["0008-4026","1480-3305"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310312929","host_organization_name":"Canadian Science Publishing","host_organization_lineage":["https://openalex.org/P4310312929"],"host_organization_lineage_names":["Canadian Science Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Canadian Journal of Botany","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.9208999872207642}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334593","display_name":"Natural Sciences and Engineering Research Council of Canada","ror":"https://ror.org/01h531d29"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":9,"referenced_works":["https://openalex.org/W1986165669","https://openalex.org/W1989771623","https://openalex.org/W1993576967","https://openalex.org/W2014773593","https://openalex.org/W2021931779","https://openalex.org/W2031534351","https://openalex.org/W2051739256","https://openalex.org/W2154123969","https://openalex.org/W2761346672"],"related_works":["https://openalex.org/W2077789156","https://openalex.org/W2151476219","https://openalex.org/W2354254275","https://openalex.org/W2356693214","https://openalex.org/W1556368690","https://openalex.org/W2317066050","https://openalex.org/W2390698592","https://openalex.org/W2154085826","https://openalex.org/W1514704735","https://openalex.org/W2380068422"],"abstract_inverted_index":{"The":[0],"effect":[1,75,90],"of":[2,20,37,69,82,94,137,143],"naphthaleneacetic":[3],"acid":[4],"(NAA),":[5],"an":[6],"auxin,":[7],"and":[8],"N6-benzyladenine":[9],"(BA),":[10],"a":[11,28,42,134,140],"cytokinin,":[12],"on":[13,76,91,103,111],"microspore":[14],"embryogenesis":[15],"in":[16,41,48,64],"two":[17],"F1":[18],"(hybrids":[19],"rapeseed":[21],"(Brassica":[22],"napus":[23],"L.)":[24],"was":[25],"investigated,":[26],"using":[27],"two-factor,":[29],"central-composite":[30],"response":[31],"surface":[32],"design.":[33],"Total":[34],"embryo":[35,77,129],"yields":[36,57,130],"both":[38],"hybrids":[39],"increased":[40],"log-linear":[43],"fashion":[44],"with":[45,133],"BA":[46,65],"concentrations":[47,68,83],"the":[49,80,92,120],"range":[50,81],"0.01\u20130.255":[51],"mg":[52,71],"L\u22121;":[53],"within":[54,146],"this":[55],"range,":[56],"approximately":[58],"doubled":[59],"for":[60],"every":[61],"fivefold":[62],"increase":[63],"concentration.":[66],"NAA":[67],"0.136\u20131.85":[70],"L\u22121":[72],"had":[73,88],"no":[74,108],"yields.":[78],"Within":[79],"studied,":[84],"neither":[85],"growth":[86],"substance":[87],"any":[89],"proportion":[93],"cotyledonary":[95,99],"embryos":[96,100],"produced.":[97],"When":[98],"were":[101,131],"regenerated":[102],"B5":[104],"medium":[105],"without":[106],"hormones,":[107],"significant":[109],"effects":[110],"either":[112],"root":[113],"development":[114],"or":[115],"shoot":[116],"production":[117],"attributable":[118],"to":[119],"NAA\u2013BA":[121],"treatments":[122],"could":[123],"be":[124],"detected.":[125],"In":[126],"general,":[127],"higher":[128],"associated":[132],"slower":[135],"rate":[136],"development,":[138],"but":[139],"greater":[141],"degree":[142],"morphological":[144],"synchrony":[145],"individual":[147],"cultures.":[148]},"counts_by_year":[{"year":2021,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}