{"id":"https://openalex.org/W2024937427","doi":"https://doi.org/10.1006/prep.2001.1555","title":"Using Secretion to Solve a Solubility Problem: High-Yield Expression in Escherichia coli and Purification of the Bacterial Glycoamidase PNGase F","display_name":"Using Secretion to Solve a Solubility Problem: High-Yield Expression in Escherichia coli and Purification of the Bacterial Glycoamidase PNGase F","publication_year":2002,"publication_date":"2002-02-01","ids":{"openalex":"https://openalex.org/W2024937427","doi":"https://doi.org/10.1006/prep.2001.1555","mag":"2024937427","pmid":"https://pubmed.ncbi.nlm.nih.gov/11812228"},"language":"en","primary_location":{"id":"doi:10.1006/prep.2001.1555","doi":"https://doi.org/10.1006/prep.2001.1555","is_oa":false,"landing_page_url":"https://doi.org/10.1006/prep.2001.1555","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5069764771","display_name":"Trevor S. Loo","orcid":"https://orcid.org/0000-0002-0501-5522","observed_orcids":["https://orcid.org/0000-0002-0501-5522"]},"institutions":[{"id":"https://openalex.org/I51158804","display_name":"Massey University","ror":"https://ror.org/052czxv31","country_code":"NZ","type":"education","lineage":["https://openalex.org/I51158804"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Trevor Loo","raw_affiliation_strings":["Institute of Molecular Biosciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Molecular Biosciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand","institution_ids":["https://openalex.org/I51158804"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036334252","display_name":"Mark L. Patchett","orcid":"https://orcid.org/0000-0002-1928-0431","observed_orcids":["https://orcid.org/0000-0002-1928-0431"]},"institutions":[{"id":"https://openalex.org/I51158804","display_name":"Massey University","ror":"https://ror.org/052czxv31","country_code":"NZ","type":"education","lineage":["https://openalex.org/I51158804"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Mark L. Patchett","raw_affiliation_strings":["Institute of Molecular Biosciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Molecular Biosciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand","institution_ids":["https://openalex.org/I51158804"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012349080","display_name":"Gillian E. Norris","orcid":"https://orcid.org/0000-0001-9231-4389","observed_orcids":["https://orcid.org/0000-0001-9231-4389"]},"institutions":[{"id":"https://openalex.org/I51158804","display_name":"Massey University","ror":"https://ror.org/052czxv31","country_code":"NZ","type":"education","lineage":["https://openalex.org/I51158804"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Gillian E. Norris","raw_affiliation_strings":["Institute of Molecular Biosciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Molecular Biosciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand","institution_ids":["https://openalex.org/I51158804"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5044485902","display_name":"J. Shaun Lott","orcid":"https://orcid.org/0000-0003-3660-452X","observed_orcids":["https://orcid.org/0000-0003-3660-452X"]},"institutions":[{"id":"https://openalex.org/I51158804","display_name":"Massey University","ror":"https://ror.org/052czxv31","country_code":"NZ","type":"education","lineage":["https://openalex.org/I51158804"]}],"countries":["NZ"],"is_corresponding":true,"raw_author_name":"J.Shaun Lott","raw_affiliation_strings":["Institute of Molecular Biosciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Molecular Biosciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand","institution_ids":["https://openalex.org/I51158804"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5044485902"],"corresponding_institution_ids":["https://openalex.org/I51158804"],"apc_list":{"value":3680,"currency":"USD","value_usd":3680},"apc_paid":null,"fwci":0.5736,"has_fulltext":false,"cited_by_count":61,"citation_normalized_percentile":{"value":0.59299921,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"24","issue":"1","first_page":"90","last_page":"98"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.48750001192092896,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.48750001192092896,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.41260001063346863,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.02459999918937683,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pngase-f","display_name":"PNGase F","score":1.0},{"id":"https://openalex.org/keywords/elizabethkingia-meningoseptica","display_name":"Elizabethkingia meningoseptica","score":0.9660000205039978},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7900000214576721},{"id":"https://openalex.org/keywords/t7-promoter","display_name":"T7 promoter","score":0.6389999985694885},{"id":"https://openalex.org/keywords/periplasmic-protein-secretion","display_name":"periplasmic secretion","score":0.843999981880188},{"id":"https://openalex.org/keywords/hexahistidine-tag","display_name":"hexahistidine tag","score":0.8479999899864197},{"id":"https://openalex.org/keywords/immobilized-metal-affinity-chromatography","display_name":"immobilized metal affinity chromatography","score":0.9100000262260437},{"id":"https://openalex.org/keywords/protein-solubility","display_name":"protein solubility","score":0.7990000247955322},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.4440000057220459}],"concepts":[{"id":"https://openalex.org/C201663137","wikidata":"https://www.wikidata.org/wiki/Q21198990","display_name":"Periplasmic space","level":4,"score":0.9253116250038147},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.8721857666969299},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.683327853679657},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6081382632255554},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6006255745887756},{"id":"https://openalex.org/C2778106697","wikidata":"https://www.wikidata.org/wiki/Q140572","display_name":"Flavobacterium","level":4,"score":0.588411808013916},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5617635846138},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.4785519242286682},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.4713275730609894},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4321490526199341},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4174484312534332},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40389472246170044},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.3667718172073364},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2285420000553131},{"id":"https://openalex.org/C2781463711","wikidata":"https://www.wikidata.org/wiki/Q131143","display_name":"Pseudomonas","level":3,"score":0.06642863154411316},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000581","descriptor_name":"Amidohydrolases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000581","descriptor_name":"Amidohydrolases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000581","descriptor_name":"Amidohydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001425","descriptor_name":"Bacterial Outer Membrane Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005417","descriptor_name":"Flavobacterium","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D005417","descriptor_name":"Flavobacterium","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012995","descriptor_name":"Solubility","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D043524","descriptor_name":"Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1006/prep.2001.1555","doi":"https://doi.org/10.1006/prep.2001.1555","is_oa":false,"landing_page_url":"https://doi.org/10.1006/prep.2001.1555","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},{"id":"pmid:11812228","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11812228","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein expression and purification","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320816","display_name":"Massey University","ror":"https://ror.org/052czxv31"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W1497028439","https://openalex.org/W1525638648","https://openalex.org/W1535133733","https://openalex.org/W1558542220","https://openalex.org/W1571594249","https://openalex.org/W1577834054","https://openalex.org/W1873199501","https://openalex.org/W1983457088","https://openalex.org/W2017438073","https://openalex.org/W2019509563","https://openalex.org/W2040648668","https://openalex.org/W2064504648","https://openalex.org/W2068518785","https://openalex.org/W2070543174","https://openalex.org/W2076452068","https://openalex.org/W2089341748","https://openalex.org/W2093929400","https://openalex.org/W2128635872","https://openalex.org/W2144634347","https://openalex.org/W2157924633","https://openalex.org/W2313118371","https://openalex.org/W2314225431"],"related_works":["https://openalex.org/W2383411385","https://openalex.org/W4214812524","https://openalex.org/W2948813093","https://openalex.org/W2611025307","https://openalex.org/W1571594249","https://openalex.org/W7159959658","https://openalex.org/W2039244427","https://openalex.org/W55688516","https://openalex.org/W4320489954","https://openalex.org/W2333528559"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}