{"id":"https://openalex.org/W2024919579","doi":"https://doi.org/10.1076/apab.105.1.10.13142","title":"Electrophoretic Separation of Myosin Heavy Chain Isoforms in the Human M. Vastus Lateralis: References to Reproducibility and Relationships with Force, Electromechanical Delay, Fibre Conduction Velocity, Endurance and Electromyography","display_name":"Electrophoretic Separation of Myosin Heavy Chain Isoforms in the Human M. Vastus Lateralis: References to Reproducibility and Relationships with Force, Electromechanical Delay, Fibre Conduction Velocity, Endurance and Electromyography","publication_year":1997,"publication_date":"1997-01-01","ids":{"openalex":"https://openalex.org/W2024919579","doi":"https://doi.org/10.1076/apab.105.1.10.13142","mag":"2024919579","pmid":"https://pubmed.ncbi.nlm.nih.gov/9224541"},"language":"en","primary_location":{"id":"doi:10.1076/apab.105.1.10.13142","doi":"https://doi.org/10.1076/apab.105.1.10.13142","is_oa":false,"landing_page_url":"https://doi.org/10.1076/apab.105.1.10.13142","pdf_url":null,"source":{"id":"https://openalex.org/S19783875","display_name":"Archives of Physiology and Biochemistry","issn_l":"0778-3124","issn":["0778-3124","1381-3455","1744-4160"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of Physiology and Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016981749","display_name":"A. D. Taylor","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I66809481","display_name":"Southern Cross University","ror":"https://ror.org/001xkv632","country_code":"AU","type":"education","lineage":["https://openalex.org/I66809481"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"A. D. Taylor","raw_affiliation_strings":["Southern Cross University, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Southern Cross University, New South Wales, Australia","institution_ids":["https://openalex.org/I66809481"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060654318","display_name":"Brendan J. Humphries","orcid":"https://orcid.org/0000-0003-0153-3629","observed_orcids":["https://orcid.org/0000-0003-0153-3629"]},"institutions":[{"id":"https://openalex.org/I66809481","display_name":"Southern Cross University","ror":"https://ror.org/001xkv632","country_code":"AU","type":"education","lineage":["https://openalex.org/I66809481"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"B. Humphries","raw_affiliation_strings":["Southern Cross University, Lismore 2480, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Southern Cross University, Lismore 2480, New South Wales, Australia","institution_ids":["https://openalex.org/I66809481"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016499925","display_name":"Paul N. Smith","orcid":"https://orcid.org/0000-0001-6891-5694","observed_orcids":["https://orcid.org/0000-0001-6891-5694"]},"institutions":[{"id":"https://openalex.org/I66809481","display_name":"Southern Cross University","ror":"https://ror.org/001xkv632","country_code":"AU","type":"education","lineage":["https://openalex.org/I66809481"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"P. Smith","raw_affiliation_strings":["Southern Cross University, Lismore 2480, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Southern Cross University, Lismore 2480, New South Wales, Australia","institution_ids":["https://openalex.org/I66809481"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5105775062","display_name":"Roger Bronks","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I66809481","display_name":"Southern Cross University","ror":"https://ror.org/001xkv632","country_code":"AU","type":"education","lineage":["https://openalex.org/I66809481"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"R. Bronks","raw_affiliation_strings":["Southern Cross University, Lismore 2480, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Southern Cross University, Lismore 2480, New South Wales, Australia","institution_ids":["https://openalex.org/I66809481"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I66809481"],"apc_list":null,"apc_paid":null,"fwci":0.0992,"has_fulltext":false,"cited_by_count":31,"citation_normalized_percentile":{"value":0.32156679,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"105","issue":"1","first_page":"10","last_page":"18"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.4171999990940094,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.4171999990940094,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10784","display_name":"Muscle activation and electromyography studies","score":0.24240000545978546,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11209","display_name":"Cardiovascular and exercise physiology","score":0.14429999887943268,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/myosin-heavy-chain-isoforms","display_name":"myosin heavy chain isoforms","score":0.9959999918937683},{"id":"https://openalex.org/keywords/vastus-lateralis","display_name":"vastus lateralis","score":0.8669999837875366},{"id":"https://openalex.org/keywords/muscle-fiber-type-distribution","display_name":"muscle fiber type distribution","score":0.7409999966621399},{"id":"https://openalex.org/keywords/reproducibility","display_name":"reproducibility","score":0.6069999933242798},{"id":"https://openalex.org/keywords/muscle-fiber-conduction-velocity","display_name":"muscle fiber conduction velocity","score":0.8270000219345093},{"id":"https://openalex.org/keywords/electromechanical-delay","display_name":"electromechanical delay","score":0.996999979019165},{"id":"https://openalex.org/keywords/rate-of-force-development","display_name":"rate of force development","score":0.9129999876022339},{"id":"https://openalex.org/keywords/isometric-force","display_name":"isometric force","score":0.6949999928474426},{"id":"https://openalex.org/keywords/muscular-endurance","display_name":"muscular endurance","score":0.7749999761581421},{"id":"https://openalex.org/keywords/electromyography","display_name":"electromyography","score":0.7710000276565552},{"id":"https://openalex.org/keywords/muscle-performance","display_name":"muscle performance","score":0.4350000023841858},{"id":"https://openalex.org/keywords/muscle-biopsy","display_name":"muscle biopsy","score":0.3160000145435333}],"concepts":[{"id":"https://openalex.org/C9893847","wikidata":"https://www.wikidata.org/wiki/Q1425625","display_name":"Reproducibility","level":2,"score":0.7279576659202576},{"id":"https://openalex.org/C2777515770","wikidata":"https://www.wikidata.org/wiki/Q507369","display_name":"Electromyography","level":2,"score":0.6723201274871826},{"id":"https://openalex.org/C179253579","wikidata":"https://www.wikidata.org/wiki/Q6276958","display_name":"Nerve conduction velocity","level":2,"score":0.6031219363212585},{"id":"https://openalex.org/C6997183","wikidata":"https://www.wikidata.org/wiki/Q271727","display_name":"Myosin","level":2,"score":0.5692774653434753},{"id":"https://openalex.org/C53345823","wikidata":"https://www.wikidata.org/wiki/Q5531554","display_name":"Gene isoform","level":3,"score":0.4592359960079193},{"id":"https://openalex.org/C2776643329","wikidata":"https://www.wikidata.org/wiki/Q975459","display_name":"Vastus lateralis muscle","level":3,"score":0.45505836606025696},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.42650914192199707},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38666731119155884},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.37015068531036377},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3648480772972107},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.3458516597747803},{"id":"https://openalex.org/C99508421","wikidata":"https://www.wikidata.org/wiki/Q2678675","display_name":"Physical medicine and rehabilitation","level":1,"score":0.32569146156311035},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.28174883127212524},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2083800733089447},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.1549552083015442},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12173184752464294},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08418932557106018},{"id":"https://openalex.org/C2779959927","wikidata":"https://www.wikidata.org/wiki/Q1048687","display_name":"Skeletal muscle","level":2,"score":0.06701716780662537}],"mesh":[{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004553","descriptor_name":"Electric Conductivity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004576","descriptor_name":"Electromyography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007525","descriptor_name":"Isoelectric Focusing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007537","descriptor_name":"Isometric Contraction","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009119","descriptor_name":"Muscle Contraction","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D010807","descriptor_name":"Physical Endurance","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018485","descriptor_name":"Muscle Fibers, Skeletal","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D018995","descriptor_name":"Myosin Heavy Chains","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D018995","descriptor_name":"Myosin Heavy Chains","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1076/apab.105.1.10.13142","doi":"https://doi.org/10.1076/apab.105.1.10.13142","is_oa":false,"landing_page_url":"https://doi.org/10.1076/apab.105.1.10.13142","pdf_url":null,"source":{"id":"https://openalex.org/S19783875","display_name":"Archives of Physiology and Biochemistry","issn_l":"0778-3124","issn":["0778-3124","1381-3455","1744-4160"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of Physiology and Biochemistry","raw_type":"journal-article"},{"id":"pmid:9224541","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9224541","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of physiology and biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.4043000042438507}],"study_designs":[{"id":"https://openalex.org/study-designs/observational-study","display_name":"Observational Study"}],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W79621560","https://openalex.org/W1923391821","https://openalex.org/W1973323844","https://openalex.org/W1988630498","https://openalex.org/W2004370078","https://openalex.org/W2028379756","https://openalex.org/W2044535513","https://openalex.org/W2050438467","https://openalex.org/W2052661634","https://openalex.org/W2089938216","https://openalex.org/W2094315367","https://openalex.org/W2096835822","https://openalex.org/W2100837269","https://openalex.org/W2111560717","https://openalex.org/W2114432823","https://openalex.org/W2128635872","https://openalex.org/W2139985432","https://openalex.org/W2146960314","https://openalex.org/W2175991112","https://openalex.org/W2180042831","https://openalex.org/W2180793908","https://openalex.org/W2187063188","https://openalex.org/W2269135633"],"related_works":["https://openalex.org/W1990191872","https://openalex.org/W2018893486","https://openalex.org/W2048890100","https://openalex.org/W1966830850","https://openalex.org/W4256261572","https://openalex.org/W2343549558","https://openalex.org/W2042969301","https://openalex.org/W2969185127","https://openalex.org/W2018024567","https://openalex.org/W2026599733"],"abstract_inverted_index":{"The":[0,201],"aim":[1],"of":[2,23,30,43,54,76,82,90,99,105,155,166,188,198,203,246,271],"this":[3],"investigation":[4],"was":[5,234,280,328],"to":[6,308],"determine":[7],"the":[8,14,21,36,44,58,91,125,156,239],"relationship":[9],"between":[10,228,318],"muscle":[11,66,344],"performance":[12],"and":[13,20,57,85,108,132,173,181,220,231,268,305,321],"myosin":[15],"heavy":[16],"chain":[17],"(MHC)":[18],"composition":[19,223,320],"reliability":[22],"electrophoretically":[24],"determined":[25,141],"MHC":[26,136,186,214,222,314],"compositions.":[27],"A":[28],"total":[29],"thirty-one":[31],"male":[32],"subjects":[33,289],"participated":[34],"in":[35,287,343],"experiments.":[37],"Maximal":[38],"voluntary":[39],"isometric":[40,64,88,259,299,336],"contractions":[41,89,300],"(MVC)":[42],"knee":[45,52,92],"extensors":[46,93],"were":[47,61,94,114,122,140,177,192,310,325],"performed":[48],"at":[49,96,302],"an":[50,97],"arbitrary":[51],"angle":[53,98],"90":[55,100],"degrees":[56,101],"following":[59],"variables":[60],"recorded:":[62],"maximal":[63,74],"force,":[65],"fibre":[67,345],"conduction":[68],"velocity":[69],"(MFCV),":[70],"electromechanical":[71],"delay":[72],"(EMD),":[73],"rate":[75],"force":[77,260],"development":[78,261],"(MRFD),":[79],"median":[80],"frequency":[81],"EMG":[83],"(MF)":[84],"iEMG.":[86],"Static":[87],"held":[95,301],"using":[102,238],"contractile":[103],"forces":[104],"10%,":[106,184,303],"50%":[107,304],"100%":[109,306],"MVC,":[110],"respectively.":[111,185],"These":[112],"tests":[113],"conducted":[115],"on":[116],"separate":[117],"days.":[118],"Muscle":[119],"biopsy":[120,190,208],"samples":[121,191,209],"obtained":[123,334],"from":[124,161,196],"left":[126],"m.":[127,157],"vastus":[128,158],"lateralis":[129,159],"before":[130],"MVC":[131,307,337],"static":[133],"endurance":[134],"tests.":[135],"protein":[137],"isoform":[138,215],"differences":[139,342],"through":[142],"sodium":[143],"dodecyl-polyacrylamide":[144],"gel":[145],"electrophoresis":[146],"(SDS-PAGE)":[147],"followed":[148],"by":[149],"densitometric":[150,240],"analysis.":[151],"Type":[152],"I-MHC":[153,219],"compositions":[154,187],"ranged":[160],"20-68%":[162],"with":[163,243,313],"a":[164,282],"mean":[165,170],"49":[167],"+/-":[168,179,183],"18%,":[169],"type":[171,174,218,229,232,247],"IIa-MHC":[172,230],"IIb-MHC":[175,233],"percentages":[176,245],"35":[178],"16%":[180],"16":[182],"duplicate":[189,207],"not":[193,235,293,311,339],"significantly":[194,250],"different":[195],"that":[197,330],"original":[199],"samples.":[200],"coefficients":[202],"variation":[204],"calculated":[205],"for":[206,213,217,298],"suggested":[210,329],"reasonable":[211],"reproducibility":[212],"differentiation":[216],"type-II":[221],"(CV":[224],"=":[225,254,263,274],"12.6%).":[226],"Differentiation":[227],"always":[236],"clear":[237],"traces.":[241],"Subjects":[242],"higher":[244],"II-MHC":[248,319],"displayed":[249],"faster":[251,285],"MFCV":[252],"(r":[253,262,273],"0.67,":[255],"P":[256,265,276],"<":[257,266,277],"0.1),":[258],"0.68,":[264],"0.1)":[267],"shorter":[269],"periods":[270],"EMD":[272],"-0.72,":[275],"0.05).":[278],"There":[279],"also":[281],"tendency":[283],"toward":[284],"MRFD":[286],"these":[288],"although":[290],"results":[291],"did":[292,338],"reach":[294],"significance.":[295],"Endurance":[296],"times":[297],"exhaustion":[309],"correlated":[312],"composition.":[315,346],"No":[316],"relationships":[317],"MF":[322],"or":[323],"iEMG":[324],"observed.":[326],"It":[327],"surface":[331],"electromyographic":[332],"recordings":[333],"during":[335],"reflect":[340],"underlying":[341]},"counts_by_year":[{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}