{"id":"https://openalex.org/W2024913776","doi":"https://doi.org/10.1038/nbt1095-1105","title":"Human Stress Protein hsp70: Overexpression in E. coli, Purification and Characterization","display_name":"Human Stress Protein hsp70: Overexpression in E. coli, Purification and Characterization","publication_year":1995,"publication_date":"1995-10-01","ids":{"openalex":"https://openalex.org/W2024913776","doi":"https://doi.org/10.1038/nbt1095-1105","mag":"2024913776","pmid":"https://pubmed.ncbi.nlm.nih.gov/9636284"},"language":"en","primary_location":{"id":"doi:10.1038/nbt1095-1105","doi":"https://doi.org/10.1038/nbt1095-1105","is_oa":false,"landing_page_url":"https://doi.org/10.1038/nbt1095-1105","pdf_url":null,"source":{"id":"https://openalex.org/S106963461","display_name":"Nature Biotechnology","issn_l":"0733-222X","issn":["0733-222X","1087-0156","1546-1696","2331-3684"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bio/Technology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103442633","display_name":"Satish Jindal","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47834966","display_name":"Framingham State University","ror":"https://ror.org/02mp2w060","country_code":"US","type":"education","lineage":["https://openalex.org/I47834966"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Satish Jindal","raw_affiliation_strings":["PerSeptive Biosystems, Framingham, MA, USA","PerSeptive Biosystems, Framingham"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"PerSeptive Biosystems, Framingham, MA, USA","institution_ids":[]},{"raw_affiliation_string":"PerSeptive Biosystems, Framingham","institution_ids":["https://openalex.org/I47834966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019030969","display_name":"Peter J. Murray","orcid":"https://orcid.org/0000-0001-6329-9802","observed_orcids":["https://orcid.org/0000-0001-6329-9802"]},"institutions":[{"id":"https://openalex.org/I4210157710","display_name":"Whitehead Institute for Biomedical Research","ror":"https://ror.org/04vqm6w82","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210157710","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peter Murray","raw_affiliation_strings":["Whitehead Institute for Biomedical Research and Department of Biology, MIT, Cambridge, MA, 02142","Whitehead Institute for Biomedical Research and Department of Biology, MIT, Cambridge"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Whitehead Institute for Biomedical Research and Department of Biology, MIT, Cambridge, MA, 02142","institution_ids":["https://openalex.org/I4210157710","https://openalex.org/I63966007"]},{"raw_affiliation_string":"Whitehead Institute for Biomedical Research and Department of Biology, MIT, Cambridge","institution_ids":["https://openalex.org/I4210157710","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021788067","display_name":"Stanley Rosenberg","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47834966","display_name":"Framingham State University","ror":"https://ror.org/02mp2w060","country_code":"US","type":"education","lineage":["https://openalex.org/I47834966"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stanley Rosenberg","raw_affiliation_strings":["PerSeptive Biosystems, 500 Old Connecticut Path, Framingham, MA","PerSeptive Biosystems, Framingham"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"PerSeptive Biosystems, 500 Old Connecticut Path, Framingham, MA","institution_ids":[]},{"raw_affiliation_string":"PerSeptive Biosystems, Framingham","institution_ids":["https://openalex.org/I47834966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112460504","display_name":"Richard A. Young","orcid":"https://orcid.org/0000-0001-8855-8647","observed_orcids":["https://orcid.org/0000-0001-8855-8647"]},"institutions":[{"id":"https://openalex.org/I4210157710","display_name":"Whitehead Institute for Biomedical Research","ror":"https://ror.org/04vqm6w82","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210157710","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard A. Young","raw_affiliation_strings":["Whitehead Institute for Biomedical Research and Department of Biology, MIT, Cambridge, MA, 02142","Whitehead Institute for Biomedical Research and Department of Biology, MIT, Cambridge"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Whitehead Institute for Biomedical Research and Department of Biology, MIT, Cambridge, MA, 02142","institution_ids":["https://openalex.org/I4210157710","https://openalex.org/I63966007"]},{"raw_affiliation_string":"Whitehead Institute for Biomedical Research and Department of Biology, MIT, Cambridge","institution_ids":["https://openalex.org/I4210157710","https://openalex.org/I63966007"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5068362750","display_name":"Kevin P. Williams","orcid":"https://orcid.org/0000-0002-6930-4630","observed_orcids":["https://orcid.org/0000-0002-6930-4630"]},"institutions":[{"id":"https://openalex.org/I47834966","display_name":"Framingham State University","ror":"https://ror.org/02mp2w060","country_code":"US","type":"education","lineage":["https://openalex.org/I47834966"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kevin P. Williams","raw_affiliation_strings":["PerSeptive Biosystems, 500 Old Connecticut Path, Framingham, MA","PerSeptive Biosystems, Framingham"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"PerSeptive Biosystems, 500 Old Connecticut Path, Framingham, MA","institution_ids":[]},{"raw_affiliation_string":"PerSeptive Biosystems, Framingham","institution_ids":["https://openalex.org/I47834966"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":12690,"currency":"USD","value_usd":12690},"apc_paid":null,"fwci":0.1239,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.39845605,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"13","issue":"10","first_page":"1105","last_page":"1109"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hsp70","display_name":"HSP70","score":0.9670000076293945},{"id":"https://openalex.org/keywords/heat-shock-proteins","display_name":"heat shock proteins","score":0.8640000224113464},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.9259999990463257},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.7960000038146973},{"id":"https://openalex.org/keywords/inclusion-bodies","display_name":"inclusion bodies","score":0.9470000267028809},{"id":"https://openalex.org/keywords/protein-refolding","display_name":"protein refolding","score":0.8270000219345093},{"id":"https://openalex.org/keywords/dnak","display_name":"DnaK","score":0.6980000138282776},{"id":"https://openalex.org/keywords/peptide-binding","display_name":"peptide binding","score":0.7440000176429749},{"id":"https://openalex.org/keywords/atp-binding","display_name":"ATP binding","score":0.8259999752044678},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.6100000143051147},{"id":"https://openalex.org/keywords/cytoprotection","display_name":"cytoprotection","score":0.8130000233650208},{"id":"https://openalex.org/keywords/immune-response-to-infection","display_name":"immune response to infection","score":0.7559999823570251}],"concepts":[{"id":"https://openalex.org/C68991219","wikidata":"https://www.wikidata.org/wiki/Q419102","display_name":"Hsp70","level":4,"score":0.5813924074172974},{"id":"https://openalex.org/C2780841128","wikidata":"https://www.wikidata.org/wiki/Q5073781","display_name":"Characterization (materials science)","level":2,"score":0.5720507502555847},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4245888292789459},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2642144560813904},{"id":"https://openalex.org/C205260736","wikidata":"https://www.wikidata.org/wiki/Q418939","display_name":"Heat shock protein","level":3,"score":0.26212847232818604},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.21002578735351562},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.13060951232910156},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11966407299041748}],"mesh":[{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001667","descriptor_name":"Binding, Competitive","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002458","descriptor_name":"Cell Fractionation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002479","descriptor_name":"Inclusion Bodies","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012321","descriptor_name":"DNA-Directed RNA Polymerases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018840","descriptor_name":"HSP70 Heat-Shock Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D018840","descriptor_name":"HSP70 Heat-Shock Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D018840","descriptor_name":"HSP70 Heat-Shock Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1038/nbt1095-1105","doi":"https://doi.org/10.1038/nbt1095-1105","is_oa":false,"landing_page_url":"https://doi.org/10.1038/nbt1095-1105","pdf_url":null,"source":{"id":"https://openalex.org/S106963461","display_name":"Nature Biotechnology","issn_l":"0733-222X","issn":["0733-222X","1087-0156","1546-1696","2331-3684"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bio/Technology","raw_type":"journal-article"},{"id":"pmid:9636284","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9636284","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bio/technology (Nature Publishing Company)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W1520714027","https://openalex.org/W1544975911","https://openalex.org/W1569913655","https://openalex.org/W1916783277","https://openalex.org/W1958996074","https://openalex.org/W1975993749","https://openalex.org/W1978014671","https://openalex.org/W1992271142","https://openalex.org/W2034943952","https://openalex.org/W2035160839","https://openalex.org/W2043825401","https://openalex.org/W2051793369","https://openalex.org/W2054718546","https://openalex.org/W2055533988","https://openalex.org/W2060864422","https://openalex.org/W2075155315","https://openalex.org/W2075809176","https://openalex.org/W2076201930","https://openalex.org/W2101454063","https://openalex.org/W2140040046","https://openalex.org/W2240281932","https://openalex.org/W2256757652"],"related_works":["https://openalex.org/W2382825382","https://openalex.org/W2388185079","https://openalex.org/W2010744208","https://openalex.org/W2467726682","https://openalex.org/W2361848748","https://openalex.org/W2374147422","https://openalex.org/W2362304774","https://openalex.org/W2354419103","https://openalex.org/W2766967491","https://openalex.org/W4408612399"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}