{"id":"https://openalex.org/W2024869652","doi":"https://doi.org/10.1126/science.1852075","title":"A Calcium-Dependent Protein Kinase with a Regulatory Domain Similar to Calmodulin","display_name":"A Calcium-Dependent Protein Kinase with a Regulatory Domain Similar to Calmodulin","publication_year":1991,"publication_date":"1991-05-17","ids":{"openalex":"https://openalex.org/W2024869652","doi":"https://doi.org/10.1126/science.1852075","mag":"2024869652","pmid":"https://pubmed.ncbi.nlm.nih.gov/1852075"},"language":"en","primary_location":{"id":"doi:10.1126/science.1852075","doi":"https://doi.org/10.1126/science.1852075","is_oa":false,"landing_page_url":"https://doi.org/10.1126/science.1852075","pdf_url":null,"source":{"id":"https://openalex.org/S3880285","display_name":"Science","issn_l":"0036-8075","issn":["0036-8075","0193-4511","1095-9203"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315823","host_organization_name":"American Association for the Advancement of Science","host_organization_lineage":["https://openalex.org/P4310315823"],"host_organization_lineage_names":["American Association for the Advancement of Science"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015528389","display_name":"Jeffrey F. Harper","orcid":"https://orcid.org/0000-0003-0596-8590","observed_orcids":["https://orcid.org/0000-0003-0596-8590"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeffrey F. Harper","raw_affiliation_strings":["Department of Horticulture, University of Wisconsin, Madison, WI, 53706"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Horticulture, University of Wisconsin, Madison, WI, 53706","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071851175","display_name":"Michael R. Sussman","orcid":"https://orcid.org/0000-0001-9374-3353","observed_orcids":["https://orcid.org/0000-0001-9374-3353"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael R. Sussman","raw_affiliation_strings":["Department of Horticulture, University of Wisconsin, Madison, WI, 53706"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Horticulture, University of Wisconsin, Madison, WI, 53706","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075404623","display_name":"G. Eric Schaller","orcid":"https://orcid.org/0000-0003-4032-2437","observed_orcids":["https://orcid.org/0000-0003-4032-2437"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"G. Eric Schaller","raw_affiliation_strings":["Department of Horticulture, University of Wisconsin, Madison, WI, 53706"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Horticulture, University of Wisconsin, Madison, WI, 53706","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057424489","display_name":"Cindy L. Putnam-Evans","orcid":"https://orcid.org/0000-0003-3727-1245","observed_orcids":["https://orcid.org/0000-0003-3727-1245"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cindy Putnam-Evans","raw_affiliation_strings":["Biochemistry Department, University of Georgia, Athens, GA 30602"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemistry Department, University of Georgia, Athens, GA 30602","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005668168","display_name":"Harry Charbonneau","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Harry Charbonneau","raw_affiliation_strings":["Department of Biochemistry, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110856226","display_name":"Alice C. Harmon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alice C. Harmon","raw_affiliation_strings":["Department of Botany, University of Florida, Gainesville, FL 32611-2009"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Botany, University of Florida, Gainesville, FL 32611-2009","institution_ids":["https://openalex.org/I33213144"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":11.364,"has_fulltext":false,"cited_by_count":398,"citation_normalized_percentile":{"value":0.99142462,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":90,"max":100},"biblio":{"volume":"252","issue":"5008","first_page":"951","last_page":"954"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.7832000255584717,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.7832000255584717,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12030","display_name":"S100 Proteins and Annexins","score":0.056699998676776886,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12620","display_name":"Signaling Pathways in Disease","score":0.04430000111460686,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/calcium-dependent-protein-kinase","display_name":"calcium-dependent protein kinase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/ef-hand-calcium-binding-domain","display_name":"EF-hand calcium-binding domain","score":0.7570000290870667},{"id":"https://openalex.org/keywords/second-messengers","display_name":"second messengers","score":0.574999988079071},{"id":"https://openalex.org/keywords/soybean","display_name":"soybean","score":0.6549999713897705},{"id":"https://openalex.org/keywords/protein-kinase-structure","display_name":"protein kinase structure","score":0.5569999814033508},{"id":"https://openalex.org/keywords/calcium-regulation-of-enzyme-activity","display_name":"calcium regulation of enzyme activity","score":0.6060000061988831}],"concepts":[{"id":"https://openalex.org/C29688787","wikidata":"https://www.wikidata.org/wiki/Q409145","display_name":"Calmodulin","level":3,"score":0.7807509899139404},{"id":"https://openalex.org/C519063684","wikidata":"https://www.wikidata.org/wiki/Q706","display_name":"Calcium","level":2,"score":0.5821570158004761},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.5714713931083679},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.5385454893112183},{"id":"https://openalex.org/C99405784","wikidata":"https://www.wikidata.org/wiki/Q410434","display_name":"c-Raf","level":5,"score":0.5113367438316345},{"id":"https://openalex.org/C32619005","wikidata":"https://www.wikidata.org/wiki/Q24768234","display_name":"Protein kinase domain","level":4,"score":0.5087440609931946},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5031430125236511},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4883534610271454},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.46704214811325073},{"id":"https://openalex.org/C159479382","wikidata":"https://www.wikidata.org/wiki/Q18030801","display_name":"MAP2K7","level":5,"score":0.4395182728767395},{"id":"https://openalex.org/C90934575","wikidata":"https://www.wikidata.org/wiki/Q6593810","display_name":"Mitogen-activated protein kinase kinase","level":4,"score":0.4347125291824341},{"id":"https://openalex.org/C122229402","wikidata":"https://www.wikidata.org/wiki/Q5018802","display_name":"Calcium-binding protein","level":3,"score":0.42493391036987305},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.41266191005706787},{"id":"https://openalex.org/C82495950","wikidata":"https://www.wikidata.org/wiki/Q14911732","display_name":"Cyclin-dependent kinase 2","level":4,"score":0.36983659863471985},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.12540847063064575},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11877930164337158},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001921","descriptor_name":"Brain","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D002147","descriptor_name":"Calmodulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012689","descriptor_name":"Sequence Homology, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013025","descriptor_name":"Glycine max","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013025","descriptor_name":"Glycine max","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017871","descriptor_name":"Calcium-Calmodulin-Dependent Protein Kinases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1126/science.1852075","doi":"https://doi.org/10.1126/science.1852075","is_oa":false,"landing_page_url":"https://doi.org/10.1126/science.1852075","pdf_url":null,"source":{"id":"https://openalex.org/S3880285","display_name":"Science","issn_l":"0036-8075","issn":["0036-8075","0193-4511","1095-9203"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315823","host_organization_name":"American Association for the Advancement of Science","host_organization_lineage":["https://openalex.org/P4310315823"],"host_organization_lineage_names":["American Association for the Advancement of Science"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science","raw_type":"journal-article"},{"id":"pmid:1852075","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1852075","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science (New York, N.Y.)","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G455733191","display_name":null,"funder_award_id":"GM-15731","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G5475518267","display_name":"PROTEIN STUCTURE--DOMAINS AND REGULATION OF CD45","funder_award_id":"5r01gm015731-26","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W990604247","https://openalex.org/W1520034199","https://openalex.org/W1968559401","https://openalex.org/W1976272357","https://openalex.org/W1993282564","https://openalex.org/W2011914939","https://openalex.org/W2020257412","https://openalex.org/W2027351968","https://openalex.org/W2054406993","https://openalex.org/W2055252975","https://openalex.org/W2058491719","https://openalex.org/W2062947813","https://openalex.org/W2066825851","https://openalex.org/W2070589373","https://openalex.org/W2077686814","https://openalex.org/W2078517595","https://openalex.org/W2084576507","https://openalex.org/W2108388593","https://openalex.org/W2132736984","https://openalex.org/W2153705256"],"related_works":["https://openalex.org/W2047345705","https://openalex.org/W1964402826","https://openalex.org/W2084576507","https://openalex.org/W2047684232","https://openalex.org/W163259764","https://openalex.org/W1457942197","https://openalex.org/W2007245805","https://openalex.org/W2044274510","https://openalex.org/W2057755708","https://openalex.org/W2027982782"],"abstract_inverted_index":{"Calcium":[0],"can":[1],"function":[2],"as":[3,100],"a":[4,51,54,64,74,101,104],"second":[5],"messenger":[6],"through":[7],"stimulation":[8],"of":[9,58,70,87,107],"calcium-dependent":[10],"protein":[11,14,52,62,109],"kinases.":[12,110],"A":[13,39],"kinase":[15,32,45,89],"that":[16,69],"requires":[17],"calcium":[18,35,79,95],"but":[19],"not":[20],"calmodulin":[21],"or":[22],"phospholipids":[23],"for":[24,43,103],"activity":[25],"has":[26,46],"been":[27,47],"purified":[28],"from":[29],"soybean.":[30],"The":[31,84],"itself":[33],"binds":[34],"with":[36,53,77],"high":[37],"affinity.":[38],"complementary":[40],"DNA":[41],"clone":[42],"this":[44,88],"identified;":[48],"it":[49,99],"encodes":[50],"predicted":[55,85],"molecular":[56],"mass":[57],"57,175":[59],"daltons.":[60],"This":[61],"contains":[63],"catalytic":[65],"domain":[66],"similar":[67],"to":[68],"calmodulin-dependent":[71],"kinases":[72],"and":[73,97],"calmodulin-like":[75],"region":[76],"four":[78],"binding":[80,96],"domains":[81],"(EF":[82],"hands).":[83],"structure":[86],"explains":[90],"its":[91],"direct":[92],"regulation":[93],"via":[94],"establishes":[98],"prototype":[102],"new":[105],"family":[106],"calcium-regulated":[108]},"counts_by_year":[{"year":2026,"cited_by_count":8},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":10},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":14},{"year":2017,"cited_by_count":8},{"year":2016,"cited_by_count":7},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}