{"id":"https://openalex.org/W2024314729","doi":"https://doi.org/10.1002/bip.21574","title":"DNA and chromatin imaging with super\u2010resolution fluorescence microscopy based on single\u2010molecule localization","display_name":"DNA and chromatin imaging with super\u2010resolution fluorescence microscopy based on single\u2010molecule localization","publication_year":2010,"publication_date":"2010-12-23","ids":{"openalex":"https://openalex.org/W2024314729","doi":"https://doi.org/10.1002/bip.21574","mag":"2024314729","pmid":"https://pubmed.ncbi.nlm.nih.gov/21184489"},"language":"en","primary_location":{"id":"doi:10.1002/bip.21574","doi":"https://doi.org/10.1002/bip.21574","is_oa":true,"landing_page_url":"https://doi.org/10.1002/bip.21574","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bip.21574","source":{"id":"https://openalex.org/S56808608","display_name":"Biopolymers","issn_l":"0006-3525","issn":["0006-3525","1097-0282"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"journal-article"},"volume":null,"type":"review","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bip.21574","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078183420","display_name":"Cristina Flors","orcid":"https://orcid.org/0000-0001-5688-9150","observed_orcids":["https://orcid.org/0000-0001-5688-9150"]},"institutions":[{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Cristina Flors","raw_affiliation_strings":["School of Chemistry and Collaborative Optical Spectroscopy, Micromanipulation and Imaging Center (COSMIC), University of Edinburgh, Joseph Black Building, The King's Buildings, West Mains Road, EH9 3JJ Edinburgh, UK. cristina.flors@ed.ac.uk","School of Chemistry and Collaborative Optical Spectroscopy, Micromanipulation and Imaging Center (COSMIC), University of Edinburgh, Joseph Black Building, The King's Buildings, West Mains Road, EH9 3JJ Edinburgh, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemistry and Collaborative Optical Spectroscopy, Micromanipulation and Imaging Center (COSMIC), University of Edinburgh, Joseph Black Building, The King's Buildings, West Mains Road, EH9 3JJ Edinburgh, UK. cristina.flors@ed.ac.uk","institution_ids":["https://openalex.org/I98677209"]},{"raw_affiliation_string":"School of Chemistry and Collaborative Optical Spectroscopy, Micromanipulation and Imaging Center (COSMIC), University of Edinburgh, Joseph Black Building, The King's Buildings, West Mains Road, EH9 3JJ Edinburgh, UK","institution_ids":["https://openalex.org/I98677209"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5078183420"],"corresponding_institution_ids":["https://openalex.org/I98677209"],"apc_list":{"value":4150,"currency":"USD","value_usd":4150},"apc_paid":null,"fwci":0.6892,"has_fulltext":true,"cited_by_count":70,"citation_normalized_percentile":{"value":0.57857143,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"95","issue":"5","first_page":"290","last_page":"297"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/super-resolution-fluorescence-microscopy","display_name":"super-resolution fluorescence microscopy","score":0.8420000076293945},{"id":"https://openalex.org/keywords/single-molecule-localization","display_name":"single-molecule localization","score":0.9789999723434448},{"id":"https://openalex.org/keywords/dna-imaging","display_name":"DNA imaging","score":0.8960000276565552},{"id":"https://openalex.org/keywords/chromatin-imaging","display_name":"chromatin imaging","score":0.9589999914169312},{"id":"https://openalex.org/keywords/photoswitchable-fluorophores","display_name":"photoswitchable fluorophores","score":0.9190000295639038},{"id":"https://openalex.org/keywords/photoblinking","display_name":"photoblinking","score":0.7760000228881836},{"id":"https://openalex.org/keywords/minor-groove-binding","display_name":"minor groove binding","score":0.5730000138282776},{"id":"https://openalex.org/keywords/dna-probes","display_name":"DNA probes","score":0.6060000061988831}],"concepts":[{"id":"https://openalex.org/C2777324032","wikidata":"https://www.wikidata.org/wiki/Q412922","display_name":"Cyanine","level":3,"score":0.9171345233917236},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7042316198348999},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6969825029373169},{"id":"https://openalex.org/C83640560","wikidata":"https://www.wikidata.org/wiki/Q180951","display_name":"Chromatin","level":3,"score":0.6116927862167358},{"id":"https://openalex.org/C138268822","wikidata":"https://www.wikidata.org/wiki/Q1051925","display_name":"Resolution (logic)","level":2,"score":0.5303032398223877},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.5189273357391357},{"id":"https://openalex.org/C2779343474","wikidata":"https://www.wikidata.org/wiki/Q3109175","display_name":"Context (archaeology)","level":2,"score":0.5126299262046814},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4780888259410858},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.47184351086616516},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.45015543699264526},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.4406154751777649},{"id":"https://openalex.org/C141239990","wikidata":"https://www.wikidata.org/wiki/Q957423","display_name":"Superresolution","level":3,"score":0.43869549036026},{"id":"https://openalex.org/C64927066","wikidata":"https://www.wikidata.org/wiki/Q36293","display_name":"Histone","level":3,"score":0.42308810353279114},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13888275623321533},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.11944299936294556},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.11642935872077942},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.10057193040847778},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.09226486086845398},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0895099937915802},{"id":"https://openalex.org/C115961682","wikidata":"https://www.wikidata.org/wiki/Q860623","display_name":"Image (mathematics)","level":2,"score":0.07217416167259216},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002843","descriptor_name":"Chromatin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004396","descriptor_name":"Coloring Agents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007364","descriptor_name":"Intercalating Agents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055668","descriptor_name":"Photochemical Processes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057054","descriptor_name":"Molecular Imaging","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/bip.21574","doi":"https://doi.org/10.1002/bip.21574","is_oa":true,"landing_page_url":"https://doi.org/10.1002/bip.21574","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bip.21574","source":{"id":"https://openalex.org/S56808608","display_name":"Biopolymers","issn_l":"0006-3525","issn":["0006-3525","1097-0282"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"journal-article"},{"id":"pmid:21184489","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21184489","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1002/bip.21574","doi":"https://doi.org/10.1002/bip.21574","is_oa":true,"landing_page_url":"https://doi.org/10.1002/bip.21574","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bip.21574","source":{"id":"https://openalex.org/S56808608","display_name":"Biopolymers","issn_l":"0006-3525","issn":["0006-3525","1097-0282"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5762241686","display_name":"Super-resolution fluorescence microscopy of isolated and cell DNA","funder_award_id":"EP/F042248/1","funder_id":"https://openalex.org/F4320334627","funder_display_name":"Engineering and Physical Sciences Research Council"}],"funders":[{"id":"https://openalex.org/F4320334627","display_name":"Engineering and Physical Sciences Research Council","ror":"https://ror.org/0439y7842"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2024314729.pdf","grobid_xml":"https://content.openalex.org/works/W2024314729.grobid-xml"},"referenced_works_count":73,"referenced_works":["https://openalex.org/W2367276","https://openalex.org/W383205146","https://openalex.org/W1680195326","https://openalex.org/W1940465833","https://openalex.org/W1963684624","https://openalex.org/W1969176224","https://openalex.org/W1970300629","https://openalex.org/W1971144253","https://openalex.org/W1978298790","https://openalex.org/W1982389474","https://openalex.org/W1983572399","https://openalex.org/W1991815226","https://openalex.org/W1992858116","https://openalex.org/W1993933593","https://openalex.org/W1994009550","https://openalex.org/W1996426210","https://openalex.org/W2002402281","https://openalex.org/W2008987165","https://openalex.org/W2014920858","https://openalex.org/W2021347542","https://openalex.org/W2023482305","https://openalex.org/W2023726797","https://openalex.org/W2024407546","https://openalex.org/W2028070489","https://openalex.org/W2030652373","https://openalex.org/W2033281996","https://openalex.org/W2039427568","https://openalex.org/W2040046178","https://openalex.org/W2047027620","https://openalex.org/W2049919990","https://openalex.org/W2051365199","https://openalex.org/W2051869246","https://openalex.org/W2054455698","https://openalex.org/W2059539741","https://openalex.org/W2060409425","https://openalex.org/W2062015983","https://openalex.org/W2062552264","https://openalex.org/W2066521860","https://openalex.org/W2067333963","https://openalex.org/W2075369115","https://openalex.org/W2075485797","https://openalex.org/W2077162713","https://openalex.org/W2081938371","https://openalex.org/W2090860493","https://openalex.org/W2096645637","https://openalex.org/W2096794302","https://openalex.org/W2099348299","https://openalex.org/W2101336703","https://openalex.org/W2103241853","https://openalex.org/W2106316394","https://openalex.org/W2106395637","https://openalex.org/W2108702314","https://openalex.org/W2109281145","https://openalex.org/W2109962207","https://openalex.org/W2110317284","https://openalex.org/W2111261502","https://openalex.org/W2113774034","https://openalex.org/W2120529762","https://openalex.org/W2122179897","https://openalex.org/W2122265398","https://openalex.org/W2124636666","https://openalex.org/W2126616934","https://openalex.org/W2126961173","https://openalex.org/W2146617182","https://openalex.org/W2152962874","https://openalex.org/W2154961704","https://openalex.org/W2156853807","https://openalex.org/W2157537668","https://openalex.org/W2160131892","https://openalex.org/W2162915475","https://openalex.org/W2775728836","https://openalex.org/W4231350070","https://openalex.org/W4255557325"],"related_works":["https://openalex.org/W1663606074","https://openalex.org/W2071501673","https://openalex.org/W2900933305","https://openalex.org/W2510779431","https://openalex.org/W2075754733","https://openalex.org/W7221655023","https://openalex.org/W2050668820","https://openalex.org/W2034554412","https://openalex.org/W6945332874","https://openalex.org/W3111344815"],"abstract_inverted_index":{"With":[0],"the":[1,14,21,107,113,121],"expansion":[2],"of":[3,16,34,47,93,99,110,123],"super-resolution":[4,32,97],"fluorescence":[5],"microscopy":[6],"methods,":[7],"it":[8,166],"is":[9,50,162,167],"now":[10],"possible":[11],"to":[12,85,115,120,169],"access":[13],"organization":[15],"cells":[17,177],"and":[18,36,125,158,165,178],"materials":[19],"at":[20],"nanoscale":[22],"by":[23,59],"optical":[24],"means.":[25],"This":[26],"review":[27],"discusses":[28],"recent":[29,130],"progress":[30],"in":[31,64,77,135,155,176],"imaging":[33,98],"isolated":[35,87],"cell":[37,101],"DNA":[38,62,88,111,146,174],"using":[39],"single-molecule":[40,159],"localization":[41,160],"methods.":[42],"A":[43],"high":[44,140],"labeling":[45,122,142],"density":[46,141],"photoswitchable":[48],"fluorophores":[49],"crucial":[51],"for":[52],"these":[53],"techniques,":[54],"which":[55,65],"can":[56,68,82],"be":[57,69,83],"provided":[58],"sequence":[60],"independent":[61],"stains":[63],"photoblinking":[66],"reactions":[67],"induced.":[70],"In":[71],"particular,":[72],"unsymmetrical":[73],"cyanine":[74,103],"intercalating":[75],"dyes":[76,104],"combination":[78],"with":[79,89,148],"special":[80],"buffers":[81],"used":[84],"image":[86],"a":[90,117],"spatial":[91],"resolution":[92],"30-40":[94],"nm.":[95],"For":[96],"chromatin,":[100],"permeant":[102],"that":[105,132],"bind":[106],"minor":[108],"groove":[109],"have":[112],"potential":[114],"become":[116],"useful":[118],"alternative":[119],"histones":[124],"other":[126],"DNA-associated":[127],"proteins.":[128],"Other":[129],"developments":[131],"are":[133,151],"interesting":[134],"this":[136],"context":[137],"such":[138],"as":[139],"methods":[143],"or":[144],"new":[145],"probes":[147],"photoswitching":[149],"functionalities":[150],"also":[152],"surveyed.":[153],"Progress":[154],"labeling,":[156],"optics,":[157],"algorithms":[161],"being":[163],"rapid,":[164],"likely":[168],"provide":[170],"real":[171],"insight":[172],"into":[173],"structuring":[175],"materials.":[179]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":7},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":10},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":10},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}