{"id":"https://openalex.org/W2023800563","doi":"https://doi.org/10.1073/pnas.0537177100","title":"An engineered two-iron superoxide reductase lacking the [Fe(SCys) 4 ] site retains its catalytic properties in vitro and in vivo","display_name":"An engineered two-iron superoxide reductase lacking the [Fe(SCys) 4 ] site retains its catalytic properties in vitro and in vivo","publication_year":2003,"publication_date":"2003-03-13","ids":{"openalex":"https://openalex.org/W2023800563","doi":"https://doi.org/10.1073/pnas.0537177100","mag":"2023800563","pmid":"https://pubmed.ncbi.nlm.nih.gov/12637682","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/153002"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.0537177100","doi":"https://doi.org/10.1073/pnas.0537177100","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.0537177100","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/153002","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5043817051","display_name":"Joseph P. Emerson","orcid":"https://orcid.org/0000-0002-7556-1393","observed_orcids":["https://orcid.org/0000-0002-7556-1393"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]},{"id":"https://openalex.org/I200870766","display_name":"Brookhaven National Laboratory","ror":"https://ror.org/02ex6cf31","country_code":"US","type":"facility","lineage":["https://openalex.org/I1330989302","https://openalex.org/I200870766","https://openalex.org/I39565521","https://openalex.org/I4210142672"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joseph P. Emerson","raw_affiliation_strings":["Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30605; and Chemistry Department, Brookhaven National Laboratory, Upton, NY 11972"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30605; and Chemistry Department, Brookhaven National Laboratory, Upton, NY 11972","institution_ids":["https://openalex.org/I165733156","https://openalex.org/I200870766"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033392303","display_name":"Diane E. Cabelli","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]},{"id":"https://openalex.org/I200870766","display_name":"Brookhaven National Laboratory","ror":"https://ror.org/02ex6cf31","country_code":"US","type":"facility","lineage":["https://openalex.org/I1330989302","https://openalex.org/I200870766","https://openalex.org/I39565521","https://openalex.org/I4210142672"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Diane E. Cabelli","raw_affiliation_strings":["Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30605; and Chemistry Department, Brookhaven National Laboratory, Upton, NY 11972"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30605; and Chemistry Department, Brookhaven National Laboratory, Upton, NY 11972","institution_ids":["https://openalex.org/I165733156","https://openalex.org/I200870766"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055112988","display_name":"Donald M. Jr. Kurtz","orcid":"https://orcid.org/0000-0003-1179-1875","observed_orcids":["https://orcid.org/0000-0003-1179-1875"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]},{"id":"https://openalex.org/I200870766","display_name":"Brookhaven National Laboratory","ror":"https://ror.org/02ex6cf31","country_code":"US","type":"facility","lineage":["https://openalex.org/I1330989302","https://openalex.org/I200870766","https://openalex.org/I39565521","https://openalex.org/I4210142672"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Donald M. Kurtz","raw_affiliation_strings":["Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30605; and Chemistry Department, Brookhaven National Laboratory, Upton, NY 11972"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30605; and Chemistry Department, Brookhaven National Laboratory, Upton, NY 11972","institution_ids":["https://openalex.org/I165733156","https://openalex.org/I200870766"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.275,"has_fulltext":false,"cited_by_count":45,"citation_normalized_percentile":{"value":0.93501563,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"100","issue":"7","first_page":"3802","last_page":"3807"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11626","display_name":"Metalloenzymes and iron-sulfur proteins","score":0.6298999786376953,"subfield":{"id":"https://openalex.org/subfields/2105","display_name":"Renewable Energy, Sustainability and the Environment"},"field":{"id":"https://openalex.org/fields/21","display_name":"Energy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11626","display_name":"Metalloenzymes and iron-sulfur proteins","score":0.6298999786376953,"subfield":{"id":"https://openalex.org/subfields/2105","display_name":"Renewable Energy, Sustainability and the Environment"},"field":{"id":"https://openalex.org/fields/21","display_name":"Energy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.353300005197525,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.007000000216066837,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/superoxide-dismutase","display_name":"superoxide dismutase","score":0.652999997138977},{"id":"https://openalex.org/keywords/desulfovibrio-vulgaris","display_name":"Desulfovibrio vulgaris","score":0.8899999856948853},{"id":"https://openalex.org/keywords/cysteine-to-serine-mutation","display_name":"cysteine-to-serine substitution","score":0.7979999780654907},{"id":"https://openalex.org/keywords/superoxide-reduction","display_name":"superoxide reduction","score":0.5899999737739563},{"id":"https://openalex.org/keywords/hydrogen-peroxide-production","display_name":"hydrogen peroxide production","score":0.7329999804496765},{"id":"https://openalex.org/keywords/electron-transfer","display_name":"electron transfer","score":0.7940000295639038},{"id":"https://openalex.org/keywords/iron-sulfur-proteins","display_name":"iron-sulfur proteins","score":0.5860000252723694},{"id":"https://openalex.org/keywords/protein-engineering","display_name":"protein engineering","score":0.5630000233650208}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6140532493591309},{"id":"https://openalex.org/C2780795997","wikidata":"https://www.wikidata.org/wiki/Q413283","display_name":"Superoxide","level":3,"score":0.49184489250183105},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.4394957423210144},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4031603932380676},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3330874443054199},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.18842357397079468}],"mesh":[{"descriptor_ui":"D000332","descriptor_name":"Aerobiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001105","descriptor_name":"Archaea","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001421","descriptor_name":"Bacteria, Anaerobic","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D007506","descriptor_name":"Iron-Sulfur Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007506","descriptor_name":"Iron-Sulfur Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009249","descriptor_name":"NADP","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019943","descriptor_name":"Amino Acid Substitution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1073/pnas.0537177100","doi":"https://doi.org/10.1073/pnas.0537177100","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.0537177100","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:153002","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/153002","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:12637682","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12637682","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:153002","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/153002","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1400612289","display_name":null,"funder_award_id":"GM 40388","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G5459687547","display_name":"Kurtz NIH","funder_award_id":"R01GM040388","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":48,"referenced_works":["https://openalex.org/W1509038183","https://openalex.org/W1563233722","https://openalex.org/W1582274650","https://openalex.org/W1675570769","https://openalex.org/W1940436875","https://openalex.org/W1972711039","https://openalex.org/W1982717633","https://openalex.org/W1984994687","https://openalex.org/W1997304332","https://openalex.org/W2005282616","https://openalex.org/W2007260948","https://openalex.org/W2011747966","https://openalex.org/W2014730205","https://openalex.org/W2017831970","https://openalex.org/W2020052486","https://openalex.org/W2021326856","https://openalex.org/W2021594222","https://openalex.org/W2022523144","https://openalex.org/W2024694966","https://openalex.org/W2032756932","https://openalex.org/W2038385241","https://openalex.org/W2045226846","https://openalex.org/W2051802937","https://openalex.org/W2052811729","https://openalex.org/W2055662172","https://openalex.org/W2060121392","https://openalex.org/W2060146407","https://openalex.org/W2060622291","https://openalex.org/W2061954574","https://openalex.org/W2069192355","https://openalex.org/W2069691395","https://openalex.org/W2076143128","https://openalex.org/W2079713548","https://openalex.org/W2106635583","https://openalex.org/W2128148887","https://openalex.org/W2145380863","https://openalex.org/W2149819076","https://openalex.org/W2150179989","https://openalex.org/W2152922502","https://openalex.org/W2162893449","https://openalex.org/W2165501777","https://openalex.org/W2168140525","https://openalex.org/W2962682223","https://openalex.org/W2964323342","https://openalex.org/W4229536270","https://openalex.org/W4247418485","https://openalex.org/W4285719527","https://openalex.org/W4298140345"],"related_works":["https://openalex.org/W2024319905","https://openalex.org/W2043667854","https://openalex.org/W7062228986","https://openalex.org/W4393368378","https://openalex.org/W1974598012","https://openalex.org/W2962682223","https://openalex.org/W2964323342","https://openalex.org/W2141989511","https://openalex.org/W1976925358","https://openalex.org/W2094587424"],"abstract_inverted_index":{"Superoxide":[0],"reductases":[1],"(SORs)":[2],"contain":[3,33],"a":[4,34,167],"characteristic":[5],"square-pyramidal":[6],"[Fe(NHis)(4)(SCys)]":[7,56,132,148,180,229],"active":[8],"site":[9,37,79,122,133,149,181,220,237],"that":[10,38,175],"catalyzes":[11],"reduction":[12,190],"of":[13,76,89,102,126,146,159,166,178,191,217,227],"superoxide":[14,143,168,194],"to":[15,27,41,110,188,195],"hydrogen":[16],"peroxide":[17],"in":[18,80,84,97,150,182,238],"several":[19],"anaerobic":[20],"bacteria":[21],"and":[22,50,55,134,142],"archaea.":[23],"Some":[24],"SORs,":[25],"referred":[26],"as":[28],"two-iron":[29],"SORs":[30],"(2Fe-SORs),":[31],"also":[32],"lower-potential":[35],"[Fe(SCys)(4)]":[36,54,78,104,121,219,236],"is":[39,199],"presumed":[40],"have":[42],"an":[43,90],"electron":[44,68],"transfer":[45,69],"function.":[46],"However,":[47],"the":[48,60,77,103,119,131,135,147,151,160,176,179,192,200,203,218,228,235],"intra-":[49],"inter-subunit":[51],"distances":[52],"between":[53,70],"iron":[57],"centers":[58],"within":[59],"2Fe-SOR":[61,94,184,207],"homodimer":[62,137],"seem":[63],"too":[64],"long":[65],"for":[66,202,234],"efficient":[67],"these":[71],"sites.":[72],"The":[73,139],"possible":[74],"role":[75],"2Fe-SORs":[81,239],"was":[82,108,185],"addressed":[83],"this":[85],"work":[86],"by":[87],"examination":[88],"engineered":[91],"Desulfovibrio":[92],"vulgaris":[93],"variant,":[95],"C13S,":[96],"which":[98],"one":[99],"ligand":[100],"residue":[101,116],"site,":[105],"cysteine":[106],"13,":[107],"changed":[109],"serine.":[111],"This":[112],"single":[113],"amino":[114],"acid":[115],"change":[117],"destroyed":[118],"native":[120],"with":[123],"complete":[124],"loss":[125],"its":[127],"iron,":[128],"but":[129],"left":[130],"protein":[136],"intact.":[138],"spectroscopic,":[140],"redox":[141],"reactivity":[144],"properties":[145],"C13S":[152,183,206],"variant":[153],"were":[154],"nearly":[155],"indistinguishable":[156],"from":[157],"those":[158],"wild-type":[161,204],"2Fe-SOR.":[162],"Aerobic":[163],"growth":[164],"complementation":[165],"dismutase":[169],"(SOD)-deficient":[170],"Escherichia":[171],"coli":[172],"strain":[173],"showed":[174],"presence":[177],"apparently":[186],"sufficient":[187],"catalyze":[189],"intracellular":[193],"nonlethal":[196],"levels.":[197],"As":[198],"case":[201],"protein,":[205],"did":[208,221],"not":[209,222],"show":[210],"any":[211],"detectable":[212],"SOD":[213,225],"activity,":[214],"i.e.,":[215],"destruction":[216],"unmask":[223],"latent":[224],"activity":[226],"site.":[230],"Possible":[231],"alternative":[232],"roles":[233],"are":[240],"considered.":[241]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}