{"id":"https://openalex.org/W2023208024","doi":"https://doi.org/10.1016/s0022-1759(00)00216-7","title":"Dependence of T cell activation on area of contact and density of a ligand-coated surface","display_name":"Dependence of T cell activation on area of contact and density of a ligand-coated surface","publication_year":2000,"publication_date":"2000-07-01","ids":{"openalex":"https://openalex.org/W2023208024","doi":"https://doi.org/10.1016/s0022-1759(00)00216-7","mag":"2023208024","pmid":"https://pubmed.ncbi.nlm.nih.gov/10915852"},"language":"en","primary_location":{"id":"doi:10.1016/s0022-1759(00)00216-7","doi":"https://doi.org/10.1016/s0022-1759(00)00216-7","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0022-1759(00)00216-7","pdf_url":null,"source":{"id":"https://openalex.org/S201669150","display_name":"Journal of Immunological Methods","issn_l":"0022-1759","issn":["0022-1759","1872-7905"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Immunological Methods","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"S.M Patrick","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S.M Patrick","raw_affiliation_strings":["Department of Chemical Engineering, Columbia University, New York, NY 10027, USA","Department of Chemical Engineering, Columbia University, Mudd Building, Room 814, 500 W. 120th Street, New York, NY 10027, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, New York, NY 10027, USA","institution_ids":["https://openalex.org/I78577930"]},{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, Mudd Building, Room 814, 500 W. 120th Street, New York, NY 10027, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":null,"display_name":"S Kim","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S Kim","raw_affiliation_strings":["Department of Chemical Engineering, Columbia University, Mudd Building, Room 814, 500 W. 120th Street, New York, NY 10027, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, Mudd Building, Room 814, 500 W. 120th Street, New York, NY 10027, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012598657","display_name":"Ned S. Braunstein","orcid":"https://orcid.org/0009-0005-2463-3034","observed_orcids":["https://orcid.org/0009-0005-2463-3034"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"N.S Braunstein","raw_affiliation_strings":["Department of Medicine, Columbia University, New York, NY 10032, USA","Dept of Medicine, Columbia University, New York, NY 10032, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Columbia University, New York, NY 10032, USA","institution_ids":["https://openalex.org/I78577930"]},{"raw_affiliation_string":"Dept of Medicine, Columbia University, New York, NY 10032, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042152649","display_name":"James Lawrence Thomas","orcid":"https://orcid.org/0000-0002-2420-7992","observed_orcids":["https://orcid.org/0000-0002-2420-7992"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J.L Thomas","raw_affiliation_strings":["Department of Chemical Engineering, Columbia University, Mudd Building, Room 814, 500 W. 120th Street, New York, NY 10027, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, Mudd Building, Room 814, 500 W. 120th Street, New York, NY 10027, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5008427380","display_name":"Edward F. Leonard","orcid":"https://orcid.org/0000-0001-8860-8536","observed_orcids":["https://orcid.org/0000-0001-8860-8536"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"E.F Leonard","raw_affiliation_strings":["Department of Chemical Engineering, Columbia University, Mudd Building, Room 814, 500 W. 120th Street, New York, NY 10027, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, Mudd Building, Room 814, 500 W. 120th Street, New York, NY 10027, USA","institution_ids":["https://openalex.org/I78577930"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5008427380"],"corresponding_institution_ids":["https://openalex.org/I78577930"],"apc_list":{"value":2970,"currency":"USD","value_usd":2970},"apc_paid":null,"fwci":0.2358,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.53231723,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"241","issue":"1-2","first_page":"97","last_page":"108"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9169999957084656,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9169999957084656,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":0.03220000118017197,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.00800000037997961,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/t-cell-activation","display_name":"T cell activation","score":1.0},{"id":"https://openalex.org/keywords/anti-cd3-antibody","display_name":"anti-CD3 antibody","score":0.7990000247955322},{"id":"https://openalex.org/keywords/ligand-density","display_name":"ligand density","score":0.9200000166893005},{"id":"https://openalex.org/keywords/contact-area","display_name":"contact area","score":0.8339999914169312},{"id":"https://openalex.org/keywords/immobilized-ligands","display_name":"immobilized ligands","score":0.7950000166893005},{"id":"https://openalex.org/keywords/calcium-mobilization","display_name":"calcium mobilization","score":0.8560000061988831},{"id":"https://openalex.org/keywords/receptor-clustering","display_name":"receptor clustering","score":0.8220000267028809},{"id":"https://openalex.org/keywords/t-cell-receptor-signaling","display_name":"T cell receptor signaling","score":0.5770000219345093},{"id":"https://openalex.org/keywords/microbeads","display_name":"microbeads","score":0.4819999933242798}],"concepts":[{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.704102098941803},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5571465492248535},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5110076665878296},{"id":"https://openalex.org/C519063684","wikidata":"https://www.wikidata.org/wiki/Q706","display_name":"Calcium","level":2,"score":0.4896565079689026},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.4880455732345581},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.4237782061100006},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.4192297160625458},{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.4151792526245117},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.34097278118133545},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1729937195777893},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.12514334917068481},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.10842248797416687},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.07198962569236755}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008213","descriptor_name":"Lymphocyte Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008846","descriptor_name":"Micromanipulation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011940","descriptor_name":"Receptor Aggregation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013499","descriptor_name":"Surface Properties","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017252","descriptor_name":"CD3 Complex","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017951","descriptor_name":"Antigen Presentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020013","descriptor_name":"Calcium Signaling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0022-1759(00)00216-7","doi":"https://doi.org/10.1016/s0022-1759(00)00216-7","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0022-1759(00)00216-7","pdf_url":null,"source":{"id":"https://openalex.org/S201669150","display_name":"Journal of Immunological Methods","issn_l":"0022-1759","issn":["0022-1759","1872-7905"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Immunological Methods","raw_type":"journal-article"},{"id":"pmid:10915852","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10915852","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of immunological methods","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.6299999952316284,"display_name":"Sustainable cities and communities","id":"https://metadata.un.org/sdg/11"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W36562614","https://openalex.org/W1492670710","https://openalex.org/W1503251968","https://openalex.org/W1525503456","https://openalex.org/W1530276086","https://openalex.org/W1538720263","https://openalex.org/W1547874256","https://openalex.org/W1562441478","https://openalex.org/W1622169185","https://openalex.org/W1966286149","https://openalex.org/W2004106131","https://openalex.org/W2020939327","https://openalex.org/W2025935390","https://openalex.org/W2031011895","https://openalex.org/W2032711419","https://openalex.org/W2036377994","https://openalex.org/W2038670191","https://openalex.org/W2041360936","https://openalex.org/W2054320393","https://openalex.org/W2062097895","https://openalex.org/W2069379397","https://openalex.org/W2073263421","https://openalex.org/W2085586163","https://openalex.org/W2092643850","https://openalex.org/W2119879881","https://openalex.org/W2133931936","https://openalex.org/W2141260882","https://openalex.org/W2144711633","https://openalex.org/W2159756478","https://openalex.org/W2163295981","https://openalex.org/W2179076164","https://openalex.org/W2317114237","https://openalex.org/W2739616593"],"related_works":["https://openalex.org/W2062097895","https://openalex.org/W2038670191","https://openalex.org/W1999879260","https://openalex.org/W7058338235","https://openalex.org/W7048785419","https://openalex.org/W7004916107","https://openalex.org/W2911842963","https://openalex.org/W2053421167","https://openalex.org/W4402972306","https://openalex.org/W1975809043"],"abstract_inverted_index":null,"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}