{"id":"https://openalex.org/W2021998328","doi":"https://doi.org/10.1016/j.pep.2006.07.013","title":"Recombinant production of a VL single domain antibody in Escherichia coli and analysis of its interaction with peptostreptococcal protein L","display_name":"Recombinant production of a VL single domain antibody in Escherichia coli and analysis of its interaction with peptostreptococcal protein L","publication_year":2006,"publication_date":"2006-08-01","ids":{"openalex":"https://openalex.org/W2021998328","doi":"https://doi.org/10.1016/j.pep.2006.07.013","mag":"2021998328","pmid":"https://pubmed.ncbi.nlm.nih.gov/16949300"},"language":"en","primary_location":{"id":"doi:10.1016/j.pep.2006.07.013","doi":"https://doi.org/10.1016/j.pep.2006.07.013","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.pep.2006.07.013","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086015255","display_name":"Aimee J. Cossins","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I43439940","display_name":"University of Southampton","ror":"https://ror.org/01ryk1543","country_code":"GB","type":"education","lineage":["https://openalex.org/I43439940"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Aimee J. Cossins","raw_affiliation_strings":["School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, UK","institution_ids":["https://openalex.org/I43439940"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110638738","display_name":"Steven L. Harrison","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I43439940","display_name":"University of Southampton","ror":"https://ror.org/01ryk1543","country_code":"GB","type":"education","lineage":["https://openalex.org/I43439940"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Steven Harrison","raw_affiliation_strings":["School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, UK","institution_ids":["https://openalex.org/I43439940"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020078631","display_name":"Andrew G. Popplewell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210134054","display_name":"UCB Pharma (United Kingdom)","ror":"https://ror.org/03428qp74","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210134054","https://openalex.org/I85722119"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Andrew G. Popplewell","raw_affiliation_strings":["UCB Celltech, 216 Bath Road, Slough, SL1 4EN, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UCB Celltech, 216 Bath Road, Slough, SL1 4EN, UK","institution_ids":["https://openalex.org/I4210134054"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113505881","display_name":"Michael G. Gore","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I43439940","display_name":"University of Southampton","ror":"https://ror.org/01ryk1543","country_code":"GB","type":"education","lineage":["https://openalex.org/I43439940"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Michael G. Gore","raw_affiliation_strings":["School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, UK","institution_ids":["https://openalex.org/I43439940"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5113505881"],"corresponding_institution_ids":["https://openalex.org/I43439940"],"apc_list":{"value":3680,"currency":"USD","value_usd":3680},"apc_paid":null,"fwci":0.6004,"has_fulltext":false,"cited_by_count":21,"citation_normalized_percentile":{"value":0.6503239,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"51","issue":"2","first_page":"253","last_page":"259"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.8798999786376953,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.8798999786376953,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.11739999800920486,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nanobodies","display_name":"nanobodies","score":0.9449999928474426},{"id":"https://openalex.org/keywords/variable-light-chain","display_name":"variable light chain","score":0.7839999794960022},{"id":"https://openalex.org/keywords/kappa-light-chain","display_name":"kappa light chain","score":0.7310000061988831},{"id":"https://openalex.org/keywords/periplasmic-expression","display_name":"periplasmic expression","score":0.8420000076293945},{"id":"https://openalex.org/keywords/escherichia-coli-fermentation","display_name":"Escherichia coli fermentation","score":0.8980000019073486},{"id":"https://openalex.org/keywords/affinity-purification","display_name":"affinity purification","score":0.42399999499320984},{"id":"https://openalex.org/keywords/protein-protein-binding","display_name":"protein-protein binding","score":0.5649999976158142},{"id":"https://openalex.org/keywords/beta-sheet-domain","display_name":"beta-sheet domain","score":0.765999972820282},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.5979999899864197}],"concepts":[{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.7975422143936157},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.724449872970581},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7029386162757874},{"id":"https://openalex.org/C36394416","wikidata":"https://www.wikidata.org/wiki/Q949267","display_name":"Immunoglobulin light chain","level":3,"score":0.6015976667404175},{"id":"https://openalex.org/C201663137","wikidata":"https://www.wikidata.org/wiki/Q21198990","display_name":"Periplasmic space","level":4,"score":0.5881732106208801},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.580856204032898},{"id":"https://openalex.org/C2779881004","wikidata":"https://www.wikidata.org/wiki/Q7451876","display_name":"Sepharose","level":3,"score":0.5262452960014343},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.46287253499031067},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.3946148455142975},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.37605994939804077},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.31100428104400635},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24878880381584167},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D001666","descriptor_name":"Binding Sites, Antibody","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002942","descriptor_name":"Circular Dichroism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005470","descriptor_name":"Fluorometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007135","descriptor_name":"Immunoglobulin Variable Region","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D007135","descriptor_name":"Immunoglobulin Variable Region","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007145","descriptor_name":"Immunoglobulin kappa-Chains","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D007145","descriptor_name":"Immunoglobulin kappa-Chains","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019906","descriptor_name":"Nuclear Magnetic Resonance, Biomolecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.pep.2006.07.013","doi":"https://doi.org/10.1016/j.pep.2006.07.013","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.pep.2006.07.013","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},{"id":"pmid:16949300","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16949300","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein expression and purification","raw_type":"Journal Article"},{"id":"pmh:oai:eprints.soton.ac.uk:56669","doi":null,"is_oa":false,"landing_page_url":"https://eprints.soton.ac.uk/56669/","pdf_url":null,"source":{"id":"https://openalex.org/S4306401019","display_name":"ePrints Soton (University of Southampton)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I43439940","host_organization_name":"University of Southampton","host_organization_lineage":["https://openalex.org/I43439940"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W1963522035","https://openalex.org/W1983257260","https://openalex.org/W1993575340","https://openalex.org/W1998836855","https://openalex.org/W2010264896","https://openalex.org/W2018502894","https://openalex.org/W2020368956","https://openalex.org/W2037185277","https://openalex.org/W2042498352","https://openalex.org/W2043330120","https://openalex.org/W2048485109","https://openalex.org/W2054965883","https://openalex.org/W2060189702","https://openalex.org/W2068450390","https://openalex.org/W2076350188","https://openalex.org/W2097478805","https://openalex.org/W2111662398","https://openalex.org/W2144991441","https://openalex.org/W2260710486","https://openalex.org/W2469800729","https://openalex.org/W6693115298","https://openalex.org/W6720437934"],"related_works":["https://openalex.org/W2084396332","https://openalex.org/W7036640443","https://openalex.org/W1992673571","https://openalex.org/W7213996830","https://openalex.org/W2224652798","https://openalex.org/W178138050","https://openalex.org/W1698305623","https://openalex.org/W2803867061","https://openalex.org/W1544101325","https://openalex.org/W2469800729"],"abstract_inverted_index":null,"counts_by_year":[{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}