{"id":"https://openalex.org/W2021587345","doi":"https://doi.org/10.1021/bi0155908","title":"Long-Range Interactions in the Dimer Interface of Ornithine Decarboxylase Are Important for Enzyme Function","display_name":"Long-Range Interactions in the Dimer Interface of Ornithine Decarboxylase Are Important for Enzyme Function","publication_year":2001,"publication_date":"2001-10-10","ids":{"openalex":"https://openalex.org/W2021587345","doi":"https://doi.org/10.1021/bi0155908","mag":"2021587345","pmid":"https://pubmed.ncbi.nlm.nih.gov/11683631"},"language":"en","primary_location":{"id":"doi:10.1021/bi0155908","doi":"https://doi.org/10.1021/bi0155908","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi0155908","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"David P. Myers","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David P. Myers","raw_affiliation_strings":["Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041","Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041","institution_ids":["https://openalex.org/I867280407"]},{"raw_affiliation_string":"Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041, USA","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5114088917","display_name":"Laurie K. Jackson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Laurie K. Jackson","raw_affiliation_strings":["Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062332135","display_name":"Vinu Ipe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vinu G. Ipe","raw_affiliation_strings":["Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063466076","display_name":"Gavin E. Murphy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gavin E. Murphy","raw_affiliation_strings":["Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5008866938","display_name":"Margaret A. Phillips","orcid":"https://orcid.org/0000-0001-5250-5578","observed_orcids":["https://orcid.org/0000-0001-5250-5578"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Margaret A. Phillips","raw_affiliation_strings":["Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041","institution_ids":["https://openalex.org/I867280407"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I867280407"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.196,"has_fulltext":false,"cited_by_count":41,"citation_normalized_percentile":{"value":0.76158417,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"40","issue":"44","first_page":"13230","last_page":"13236"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11924","display_name":"Polyamine Metabolism and Applications","score":0.698199987411499,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11924","display_name":"Polyamine Metabolism and Applications","score":0.698199987411499,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.1761000007390976,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.03739999979734421,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ornithine-decarboxylase","display_name":"ornithine decarboxylase","score":1.0},{"id":"https://openalex.org/keywords/dimer-interface","display_name":"dimer interface","score":0.8289999961853027},{"id":"https://openalex.org/keywords/long-range-interactions","display_name":"long-range interactions","score":0.8399999737739563},{"id":"https://openalex.org/keywords/trypanosoma-brucei","display_name":"Trypanosoma brucei","score":0.8560000061988831},{"id":"https://openalex.org/keywords/polyamine-biosynthesis","display_name":"polyamine biosynthesis","score":0.8980000019073486},{"id":"https://openalex.org/keywords/enzyme-catalytic-efficiency","display_name":"enzyme catalytic efficiency","score":0.7229999899864197},{"id":"https://openalex.org/keywords/dimer-stability","display_name":"dimer stability","score":0.6800000071525574},{"id":"https://openalex.org/keywords/alanine-scanning","display_name":"alanine scanning","score":0.8270000219345093},{"id":"https://openalex.org/keywords/pyridoxal-5-phosphate","display_name":"pyridoxal phosphate","score":0.8629999756813049},{"id":"https://openalex.org/keywords/human-african-trypanosomiasis","display_name":"sleeping sickness","score":0.7429999709129333},{"id":"https://openalex.org/keywords/therapeutic-target","display_name":"therapeutic target","score":0.6320000290870667},{"id":"https://openalex.org/keywords/substrate-binding-site","display_name":"substrate binding site","score":0.6159999966621399},{"id":"https://openalex.org/keywords/conformational-change","display_name":"conformational change","score":0.4909999966621399}],"concepts":[{"id":"https://openalex.org/C2779546866","wikidata":"https://www.wikidata.org/wiki/Q531306","display_name":"Dimer","level":2,"score":0.7141181826591492},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.6943421363830566},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5894601941108704},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5858311653137207},{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.4937981069087982},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.48167097568511963},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.45637309551239014},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4373278021812439},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3366662263870239},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.18752002716064453},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000409","descriptor_name":"Alanine","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009955","descriptor_name":"Ornithine Decarboxylase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009955","descriptor_name":"Ornithine Decarboxylase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009955","descriptor_name":"Ornithine Decarboxylase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014346","descriptor_name":"Trypanosoma brucei brucei","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D014346","descriptor_name":"Trypanosoma brucei brucei","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014461","descriptor_name":"Ultracentrifugation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019281","descriptor_name":"Dimerization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi0155908","doi":"https://doi.org/10.1021/bi0155908","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi0155908","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:11683631","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11683631","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2044622175","display_name":"POLYAMINE AND TRYPANOTHIONE METABOLISM IN TRYPANOSOMA BRUCEI","funder_award_id":"R01AI034432","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G8310797562","display_name":"Medical Scientist Training Program at University of Texas Southwestern Med Ctr -","funder_award_id":"T32GM008014","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":8,"referenced_works":["https://openalex.org/W597159688","https://openalex.org/W1980353405","https://openalex.org/W1995687577","https://openalex.org/W2003300391","https://openalex.org/W2075770210","https://openalex.org/W2107399000","https://openalex.org/W2137355756","https://openalex.org/W4245155464"],"related_works":["https://openalex.org/W2072009878","https://openalex.org/W7096001295","https://openalex.org/W2085611935","https://openalex.org/W2058797461","https://openalex.org/W77086271","https://openalex.org/W2039367030","https://openalex.org/W1995687577","https://openalex.org/W2088798172","https://openalex.org/W1502750012","https://openalex.org/W2611379135"],"abstract_inverted_index":{"Ornithine":[0],"decarboxylase":[1],"(ODC)":[2],"is":[3,21,35,179],"a":[4,22,92,152],"pyridoxal":[5],"5'-phosphate":[6],"dependent":[7],"enzyme":[8,34,182],"that":[9,104,138,156,168],"catalyzes":[10],"the":[11,16,27,40,48,66,86,105,108,114,120,131,135,146,176,201,225,236,242],"first":[12],"committed":[13],"step":[14],"in":[15,58,98,143,200],"biosynthesis":[17],"of":[18,29,54,69,85,107,119,134,154,172,240],"polyamines.":[19],"ODC":[20,61,193,205,226],"proven":[23],"drug":[24],"target":[25],"for":[26,181,212,217],"treatment":[28],"African":[30],"sleeping":[31],"sickness.":[32],"The":[33,204],"an":[36],"obligate":[37],"homodimer,":[38],"and":[39,83,215],"two":[41],"identical":[42],"active":[43,177,202],"sites":[44],"are":[45,157,194],"formed":[46],"at":[47],"dimer":[49,55,99,206,218],"interface.":[50,115],"Alanine":[51],"scanning":[52],"mutagenesis":[53],"interface":[56,173,207],"residues":[57,71,155,174],"Trypanosoma":[59],"brucei":[60],"was":[62],"undertaken":[63],"to":[64,72,90,175,191,196,209,224,234],"determine":[65],"energetic":[67,170,238],"contribution":[68],"these":[70],"subunit":[73],"association.":[74],"Twenty-three":[75],"mutant":[76],"enzymes":[77],"were":[78,88],"analyzed":[79],"by":[80],"analytical":[81],"ultracentrifugation,":[82],"none":[84],"mutations":[87,121],"found":[89],"cause":[91],"greater":[93],"than":[94],"1":[95],"kcal/mol":[96],"decrease":[97],"stability.":[100,219],"These":[101,164],"data":[102,165],"suggest":[103],"energetics":[106],"interaction":[109],"may":[110],"be":[111,210],"distributed":[112],"across":[113],"Most":[116],"significantly,":[117],"many":[118],"had":[122],"large":[123],"effects":[124],"(DeltaDeltaG":[125],"kcat/Km":[126],">":[127],"2.5":[128],"kcal/mol)":[129],"on":[130],"catalytic":[132,213],"efficiency":[133],"enzyme.":[136],"Residues":[137],"affected":[139],"activity":[140],"included":[141],"those":[142],"or":[144],"near":[145],"substrate":[147],"binding":[148,190],"site":[149,178],"but":[150],"also":[151],"number":[153],"distant":[158],"(15-20":[159],"A)":[160],"from":[161],"this":[162],"site.":[163,203],"provide":[166],"evidence":[167],"long-range":[169],"coupling":[171],"essential":[180],"function,":[183],"even":[184],"though":[185],"structural":[186],"changes":[187,199],"upon":[188],"ligand":[189],"wild-type":[192],"limited":[195],"local":[197],"conformational":[198],"appears":[208],"optimized":[211],"function":[214,231],"not":[216],"Thus,":[220],"small":[221],"molecules":[222],"directed":[223],"interfaces":[227],"could":[228],"impact":[229],"biological":[230],"without":[232],"having":[233],"overcome":[235],"difficult":[237],"barrier":[239],"dissociating":[241],"interacting":[243],"partners.":[244]},"counts_by_year":[{"year":2025,"cited_by_count":4},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}