{"id":"https://openalex.org/W2021393094","doi":"https://doi.org/10.2174/1874091x00903010026","title":"Primary Structure Revision and Active Site Mapping of E. Coli Isoleucyl-tRNA Synthetase by Means of Maldi Mass Spectrometry","display_name":"Primary Structure Revision and Active Site Mapping of E. Coli Isoleucyl-tRNA Synthetase by Means of Maldi Mass Spectrometry","publication_year":2009,"publication_date":"2009-03-06","ids":{"openalex":"https://openalex.org/W2021393094","doi":"https://doi.org/10.2174/1874091x00903010026","mag":"2021393094","pmid":"https://pubmed.ncbi.nlm.nih.gov/19557155","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2695604"},"language":"en","primary_location":{"id":"doi:10.2174/1874091x00903010026","doi":"https://doi.org/10.2174/1874091x00903010026","is_oa":true,"landing_page_url":"https://doi.org/10.2174/1874091x00903010026","pdf_url":"https://openbiochemistryjournal.com/VOLUME/3/PAGE/26/PDF/","source":{"id":"https://openalex.org/S170372388","display_name":"The Open Biochemistry Journal","issn_l":"1874-091X","issn":["1874-091X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320079","host_organization_name":"Bentham Science Publishers","host_organization_lineage":["https://openalex.org/P4310320079"],"host_organization_lineage_names":["Bentham Science Publishers"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Open Biochemistry Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://openbiochemistryjournal.com/VOLUME/3/PAGE/26/PDF/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111806580","display_name":"Soria Baouz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210091279","display_name":"Universit\u00e9 Sorbonne Paris Nord","ror":"https://ror.org/0199hds37","country_code":"FR","type":"education","lineage":["https://openalex.org/I4210091279"]},{"id":"https://openalex.org/I4210113761","display_name":"Institut Jacques Monod","ror":"https://ror.org/02c5gc203","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I204730241","https://openalex.org/I4210096427","https://openalex.org/I4210113761"]},{"id":"https://openalex.org/I4405264447","display_name":"Universit\u00e9 Pierre-et-Marie-Curie","ror":"https://ror.org/01yvrd251","country_code":"FR","type":"education","lineage":["https://openalex.org/I39804081","https://openalex.org/I4405264447"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Soria Baouz","raw_affiliation_strings":["UPMC Univ Paris 06 et Univ Paris 13, Institut Jacques Monod (Tour 43) Laboratoire de Photobiologie Mol\u00e9culaire (CNRS-UMR 7033, BioMoCeTi), Case courrier 7592 - 2, Place Jussieu, F-75251, Paris, Cedex 05 France","BIOMOCETI - Laboratoire de Biophysique Mol\u00e9culaire Cellulaire et Tissulaire (74 rue Marcel Cachin 93000 Bobigny - France)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UPMC Univ Paris 06 et Univ Paris 13, Institut Jacques Monod (Tour 43) Laboratoire de Photobiologie Mol\u00e9culaire (CNRS-UMR 7033, BioMoCeTi), Case courrier 7592 - 2, Place Jussieu, F-75251, Paris, Cedex 05 France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210091279","https://openalex.org/I4210113761","https://openalex.org/I4405264447"]},{"raw_affiliation_string":"BIOMOCETI - Laboratoire de Biophysique Mol\u00e9culaire Cellulaire et Tissulaire (74 rue Marcel Cachin 93000 Bobigny - France)","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108493994","display_name":"Jean\u2010Marie Schmitter","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210144489","display_name":"Institut Europ\u00e9en de Chimie et Biologie","ror":"https://ror.org/04agqs597","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I15057530","https://openalex.org/I154526488","https://openalex.org/I4210128300","https://openalex.org/I4210144489"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Jean-Marie Schmitter","raw_affiliation_strings":["Institut Europ\u00e9en de Chimie et Biologie (CNRS-FRE 2247), F-33607, Pessac, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut Europ\u00e9en de Chimie et Biologie (CNRS-FRE 2247), F-33607, Pessac, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210144489"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057233578","display_name":"Lila Chenoune","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210091279","display_name":"Universit\u00e9 Sorbonne Paris Nord","ror":"https://ror.org/0199hds37","country_code":"FR","type":"education","lineage":["https://openalex.org/I4210091279"]},{"id":"https://openalex.org/I4210113761","display_name":"Institut Jacques Monod","ror":"https://ror.org/02c5gc203","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I204730241","https://openalex.org/I4210096427","https://openalex.org/I4210113761"]},{"id":"https://openalex.org/I4405264447","display_name":"Universit\u00e9 Pierre-et-Marie-Curie","ror":"https://ror.org/01yvrd251","country_code":"FR","type":"education","lineage":["https://openalex.org/I39804081","https://openalex.org/I4405264447"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Lila Chenoune","raw_affiliation_strings":["UPMC Univ Paris 06 et Univ Paris 13 , Institut Jacques Monod (Tour 43 ) Laboratoire de Photobiologie Mol\u00e9culaire (CNRS-UMR 7033 , BioMoCeTi) , Case courrier 7592 -2 , Place Jussieu , F-75251 , Paris , Cedex 05 France","BIOMOCETI - Laboratoire de Biophysique Mol\u00e9culaire Cellulaire et Tissulaire (74 rue Marcel Cachin 93000 Bobigny - France)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UPMC Univ Paris 06 et Univ Paris 13 , Institut Jacques Monod (Tour 43 ) Laboratoire de Photobiologie Mol\u00e9culaire (CNRS-UMR 7033 , BioMoCeTi) , Case courrier 7592 -2 , Place Jussieu , F-75251 , Paris , Cedex 05 France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210091279","https://openalex.org/I4210113761","https://openalex.org/I4405264447"]},{"raw_affiliation_string":"BIOMOCETI - Laboratoire de Biophysique Mol\u00e9culaire Cellulaire et Tissulaire (74 rue Marcel Cachin 93000 Bobigny - France)","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049537801","display_name":"Christian Beauvallet","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I142476485","display_name":"\u00c9cole Polytechnique","ror":"https://ror.org/05hy3tk52","country_code":"FR","type":"education","lineage":["https://openalex.org/I142476485","https://openalex.org/I4210145102"]},{"id":"https://openalex.org/I4210103255","display_name":"Laboratoire de Biologie Structurale de la Cellule","ror":"https://ror.org/01c6vgf77","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I142476485","https://openalex.org/I4210096427","https://openalex.org/I4210103255","https://openalex.org/I4210145102"]},{"id":"https://openalex.org/I4387154544","display_name":"Institut National de la Recherche Agronomique","ror":"https://ror.org/01x3gbx83","country_code":"FR","type":"government","lineage":["https://openalex.org/I4210088668","https://openalex.org/I4387154544"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Christian Beauvallet","raw_affiliation_strings":["Institut National de la Recherche Agronomique (INRA GPL) , F-78352 , Jouy-en-Josas , France","UMR 7654) , Ecole Polytechnique , Route de Saclay , F-91128 , Palaiseau , France","GPL - Unit\u00e9 de recherche g\u00e9nomique et physiologie de la lactation (78352 Jouy-en-Josas - France)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Recherche Agronomique (INRA GPL) , F-78352 , Jouy-en-Josas , France","institution_ids":["https://openalex.org/I4387154544"]},{"raw_affiliation_string":"UMR 7654) , Ecole Polytechnique , Route de Saclay , F-91128 , Palaiseau , France","institution_ids":["https://openalex.org/I142476485","https://openalex.org/I4210103255"]},{"raw_affiliation_string":"GPL - Unit\u00e9 de recherche g\u00e9nomique et physiologie de la lactation (78352 Jouy-en-Josas - France)","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108203800","display_name":"Sylvain Blanquet","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I142476485","display_name":"\u00c9cole Polytechnique","ror":"https://ror.org/05hy3tk52","country_code":"FR","type":"education","lineage":["https://openalex.org/I142476485","https://openalex.org/I4210145102"]},{"id":"https://openalex.org/I4210103255","display_name":"Laboratoire de Biologie Structurale de la Cellule","ror":"https://ror.org/01c6vgf77","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I142476485","https://openalex.org/I4210096427","https://openalex.org/I4210103255","https://openalex.org/I4210145102"]},{"id":"https://openalex.org/I4210111777","display_name":"Laboratoire de Biochimie","ror":"https://ror.org/01thw2g46","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I190752583","https://openalex.org/I2746051580","https://openalex.org/I4210111777","https://openalex.org/I4210139971","https://openalex.org/I4210161506","https://openalex.org/I98910050"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Sylvain Blanquet","raw_affiliation_strings":["Laboratoire de Biochimie (CNRS","BIOC - Laboratoire de Biochimie de l'Ecole polytechnique (Ecole Polytechnique 91128 Palaiseau - France)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire de Biochimie (CNRS","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210111777"]},{"raw_affiliation_string":"BIOC - Laboratoire de Biochimie de l'Ecole polytechnique (Ecole Polytechnique 91128 Palaiseau - France)","institution_ids":["https://openalex.org/I142476485","https://openalex.org/I4210103255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061784392","display_name":"Anne Woisard","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210091279","display_name":"Universit\u00e9 Sorbonne Paris Nord","ror":"https://ror.org/0199hds37","country_code":"FR","type":"education","lineage":["https://openalex.org/I4210091279"]},{"id":"https://openalex.org/I4210113761","display_name":"Institut Jacques Monod","ror":"https://ror.org/02c5gc203","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I204730241","https://openalex.org/I4210096427","https://openalex.org/I4210113761"]},{"id":"https://openalex.org/I4405264447","display_name":"Universit\u00e9 Pierre-et-Marie-Curie","ror":"https://ror.org/01yvrd251","country_code":"FR","type":"education","lineage":["https://openalex.org/I39804081","https://openalex.org/I4405264447"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Anne Woisard","raw_affiliation_strings":["UPMC Univ Paris 06 et Univ Paris 13 , Institut Jacques Monod (Tour 43 ) Laboratoire de Photobiologie Mol\u00e9culaire (CNRS-UMR 7033 , BioMoCeTi) , Case courrier 7592 -2 , Place Jussieu , F-75251 , Paris , Cedex 05 France","BIOMOCETI - Laboratoire de Biophysique Mol\u00e9culaire Cellulaire et Tissulaire (74 rue Marcel Cachin 93000 Bobigny - France)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UPMC Univ Paris 06 et Univ Paris 13 , Institut Jacques Monod (Tour 43 ) Laboratoire de Photobiologie Mol\u00e9culaire (CNRS-UMR 7033 , BioMoCeTi) , Case courrier 7592 -2 , Place Jussieu , F-75251 , Paris , Cedex 05 France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210091279","https://openalex.org/I4210113761","https://openalex.org/I4405264447"]},{"raw_affiliation_string":"BIOMOCETI - Laboratoire de Biophysique Mol\u00e9culaire Cellulaire et Tissulaire (74 rue Marcel Cachin 93000 Bobigny - France)","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5034093443","display_name":"Codjo Hountondji","orcid":"https://orcid.org/0000-0003-0683-4439","observed_orcids":["https://orcid.org/0000-0003-0683-4439"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210091279","display_name":"Universit\u00e9 Sorbonne Paris Nord","ror":"https://ror.org/0199hds37","country_code":"FR","type":"education","lineage":["https://openalex.org/I4210091279"]},{"id":"https://openalex.org/I4210113761","display_name":"Institut Jacques Monod","ror":"https://ror.org/02c5gc203","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I204730241","https://openalex.org/I4210096427","https://openalex.org/I4210113761"]},{"id":"https://openalex.org/I4405264447","display_name":"Universit\u00e9 Pierre-et-Marie-Curie","ror":"https://ror.org/01yvrd251","country_code":"FR","type":"education","lineage":["https://openalex.org/I39804081","https://openalex.org/I4405264447"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Codjo Hountondji","raw_affiliation_strings":["UPMC Univ Paris 06 et Univ Paris 13 , Institut Jacques Monod (Tour 43 ) Laboratoire de Photobiologie Mol\u00e9culaire (CNRS-UMR 7033 , BioMoCeTi) , Case courrier 7592 -2 , Place Jussieu , F-75251 , Paris , Cedex 05 France","Universit\u00e9 Pierre et Marie Curie (UPMC) , Laboratoire de Photobiologie Mol\u00e9culaire , ( UMR-CNRS 7033 , BioMoCeTi) Institut Jacques Monod (Tour 43 ) Case Courrier N\u00b0 7592 2 , Place Jussieu , 75251 Paris Cedex 05 , France","UPMC Univ Paris 06 et Univ Paris"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UPMC Univ Paris 06 et Univ Paris 13 , Institut Jacques Monod (Tour 43 ) Laboratoire de Photobiologie Mol\u00e9culaire (CNRS-UMR 7033 , BioMoCeTi) , Case courrier 7592 -2 , Place Jussieu , F-75251 , Paris , Cedex 05 France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210091279","https://openalex.org/I4210113761","https://openalex.org/I4405264447"]},{"raw_affiliation_string":"Universit\u00e9 Pierre et Marie Curie (UPMC) , Laboratoire de Photobiologie Mol\u00e9culaire , ( UMR-CNRS 7033 , BioMoCeTi) Institut Jacques Monod (Tour 43 ) Case Courrier N\u00b0 7592 2 , Place Jussieu , 75251 Paris Cedex 05 , France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210113761","https://openalex.org/I4405264447"]},{"raw_affiliation_string":"UPMC Univ Paris 06 et Univ Paris","institution_ids":["https://openalex.org/I4405264447"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":9,"corresponding_author_ids":["https://openalex.org/A5034093443"],"corresponding_institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210091279","https://openalex.org/I4210113761","https://openalex.org/I4405264447"],"apc_list":null,"apc_paid":null,"fwci":0.3273,"has_fulltext":true,"cited_by_count":7,"citation_normalized_percentile":{"value":0.51947802,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"03","issue":"1","first_page":"26","last_page":"38"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.0032999999821186066,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/isoleucyl-trna-synthetase","display_name":"isoleucyl-tRNA synthetase","score":0.9869999885559082},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8769999742507935},{"id":"https://openalex.org/keywords/aminoacyl-trna-synthetase","display_name":"aminoacyl-tRNA synthetase","score":0.8330000042915344},{"id":"https://openalex.org/keywords/primary-structure","display_name":"primary structure","score":0.5630000233650208},{"id":"https://openalex.org/keywords/active-site-mapping","display_name":"active site mapping","score":0.8629999756813049},{"id":"https://openalex.org/keywords/peptide-mapping","display_name":"peptide mapping","score":0.7409999966621399},{"id":"https://openalex.org/keywords/affinity-labeling","display_name":"affinity labeling","score":0.800000011920929},{"id":"https://openalex.org/keywords/substrate-analogs","display_name":"substrate analogues","score":0.5899999737739563},{"id":"https://openalex.org/keywords/atp-binding-site","display_name":"ATP binding site","score":0.503000020980835},{"id":"https://openalex.org/keywords/aminoacylation","display_name":"aminoacylation","score":0.5019999742507935}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5880937576293945},{"id":"https://openalex.org/C151238385","wikidata":"https://www.wikidata.org/wiki/Q4746444","display_name":"Aminoacylation","level":5,"score":0.5779551267623901},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.5542548894882202},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5384405255317688},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.5182711482048035},{"id":"https://openalex.org/C2777305461","wikidata":"https://www.wikidata.org/wiki/Q484940","display_name":"Isoleucine","level":4,"score":0.5125060081481934},{"id":"https://openalex.org/C2780327651","wikidata":"https://www.wikidata.org/wiki/Q27116817","display_name":"Norleucine","level":4,"score":0.4561213552951813},{"id":"https://openalex.org/C2780679169","wikidata":"https://www.wikidata.org/wiki/Q4688981","display_name":"Affinity label","level":3,"score":0.45029282569885254},{"id":"https://openalex.org/C2776580952","wikidata":"https://www.wikidata.org/wiki/Q483745","display_name":"Leucine","level":3,"score":0.44864732027053833},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.4478517472743988},{"id":"https://openalex.org/C2908540775","wikidata":"https://www.wikidata.org/wiki/Q4688981","display_name":"Affinity labeling","level":3,"score":0.436748743057251},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.4134073257446289},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.327163428068161},{"id":"https://openalex.org/C153957851","wikidata":"https://www.wikidata.org/wiki/Q201448","display_name":"Transfer RNA","level":4,"score":0.3227323293685913},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2874729633331299},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.11525359749794006},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":7,"locations":[{"id":"doi:10.2174/1874091x00903010026","doi":"https://doi.org/10.2174/1874091x00903010026","is_oa":true,"landing_page_url":"https://doi.org/10.2174/1874091x00903010026","pdf_url":"https://openbiochemistryjournal.com/VOLUME/3/PAGE/26/PDF/","source":{"id":"https://openalex.org/S170372388","display_name":"The Open Biochemistry Journal","issn_l":"1874-091X","issn":["1874-091X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320079","host_organization_name":"Bentham Science Publishers","host_organization_lineage":["https://openalex.org/P4310320079"],"host_organization_lineage_names":["Bentham Science Publishers"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Open Biochemistry Journal","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2043202","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2695604","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:19557155","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19557155","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The open biochemistry journal","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2695604","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2695604/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Open Biochem J","raw_type":"Text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.358.8213","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.358.8213","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/e9/aa/Open_Biochem_J_2009_Mar_6_3_26-38.tar.gz","raw_type":"text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.635.741","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.635.741","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://hal-univ-paris13.archives-ouvertes.fr/docs/00/76/60/34/PDF/26TOBIOCJ.pdf","raw_type":"text"},{"id":"pmh:oai:HAL:hal-00766034v1","doi":null,"is_oa":true,"landing_page_url":"https://polytechnique.hal.science/hal-00766034","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"The Open Biochemistry Journal, 2009, 3, pp.26-38. ⟨10.2174/1874091X00903010026⟩","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.2174/1874091x00903010026","doi":"https://doi.org/10.2174/1874091x00903010026","is_oa":true,"landing_page_url":"https://doi.org/10.2174/1874091x00903010026","pdf_url":"https://openbiochemistryjournal.com/VOLUME/3/PAGE/26/PDF/","source":{"id":"https://openalex.org/S170372388","display_name":"The Open Biochemistry Journal","issn_l":"1874-091X","issn":["1874-091X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320079","host_organization_name":"Bentham Science Publishers","host_organization_lineage":["https://openalex.org/P4310320079"],"host_organization_lineage_names":["Bentham Science Publishers"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Open Biochemistry Journal","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2021393094.pdf","grobid_xml":"https://content.openalex.org/works/W2021393094.grobid-xml"},"referenced_works_count":47,"referenced_works":["https://openalex.org/W41280578","https://openalex.org/W1494821596","https://openalex.org/W1495328153","https://openalex.org/W1565321325","https://openalex.org/W1571051346","https://openalex.org/W1584948953","https://openalex.org/W1773541001","https://openalex.org/W1968457477","https://openalex.org/W1979414125","https://openalex.org/W1980461282","https://openalex.org/W1983399228","https://openalex.org/W1992219961","https://openalex.org/W1997469663","https://openalex.org/W1999046250","https://openalex.org/W2003796534","https://openalex.org/W2003897992","https://openalex.org/W2004416250","https://openalex.org/W2005700051","https://openalex.org/W2014393900","https://openalex.org/W2016607610","https://openalex.org/W2018112202","https://openalex.org/W2018973701","https://openalex.org/W2019493063","https://openalex.org/W2049602933","https://openalex.org/W2051177877","https://openalex.org/W2053811117","https://openalex.org/W2057037210","https://openalex.org/W2065072126","https://openalex.org/W2068801967","https://openalex.org/W2071905887","https://openalex.org/W2073186433","https://openalex.org/W2074385758","https://openalex.org/W2078538924","https://openalex.org/W2079229201","https://openalex.org/W2081961690","https://openalex.org/W2088230790","https://openalex.org/W2089126918","https://openalex.org/W2094291868","https://openalex.org/W2107849669","https://openalex.org/W2109526373","https://openalex.org/W2161355985","https://openalex.org/W2172963534","https://openalex.org/W2411923773","https://openalex.org/W2615816587","https://openalex.org/W4238867104","https://openalex.org/W4245225680","https://openalex.org/W6998967065"],"related_works":["https://openalex.org/W7100869125","https://openalex.org/W1773541001","https://openalex.org/W4300114142","https://openalex.org/W1997469663","https://openalex.org/W2782098965","https://openalex.org/W1493313124","https://openalex.org/W2774590892","https://openalex.org/W6996223314","https://openalex.org/W2081961690","https://openalex.org/W2018973701"],"abstract_inverted_index":{"The":[0,108,304],"correct":[1],"amino":[2,167,193,305],"acid":[3,306],"sequence":[4,154],"of":[5,15,25,37,47,69,81,87,97,110,121,126,132,138,151,157,179,186,190,203,213,226,230,254,301,323,338,351,380,401,409,424],"E.":[6,170,260,324,381,410],"coli":[7,171,261,325,382,411],"isoleucyl-tRNA":[8],"synthetase":[9,181],"(IleRS)":[10],"was":[11,39,59,71,173,294],"established":[12],"by":[13,18,41,113,175,242,309],"means":[14],"peptide":[16],"mapping":[17],"MALDI":[19,243],"mass":[20,244],"spectrometry,":[21],"using":[22],"a":[23,94,210],"set":[24],"four":[26],"endoproteases":[27],"(trypsin,":[28],"LysC,":[29],"AspN":[30],"and":[31,102,134,149,199,221,248,266,273,278,292,332,343,358,365],"GluC).":[32],"Thereafter,":[33],"the":[34,48,51,55,66,75,79,84,99,129,135,139,145,152,158,180,191,204,228,234,239,251,255,302,310,320,335,346,352,361,366,376,390,397,402,421,427],"active":[35,399],"site":[36,77,160,400],"IleRS":[38,91,111,172,339,392,412],"mapped":[40],"affinity":[42,56],"labeling":[43,57,112,178],"with":[44,90,144,182,206],"reactive":[45],"analogs":[46,312],"substrates.":[49],"For":[50],"ATP":[52,140],"binding":[53,76,159,363],"site,":[54],"reagent":[58,89,122],"pyridoxal":[60,136],"5'-diphospho-5'-adenosine":[61],"(ADP-PL),":[62],"whereas":[63,263],"periodate-oxidized":[64],"tRNA(Ile),":[65],"2',3'-dialdehyde":[67],"derivative":[68],"tRNA(Ile)":[70,100,133],"used":[72],"to":[73,118,284,290,297,419],"label":[74],"for":[78,161,164],"3'-end":[80,131],"tRNA":[82],"on":[83,169,259],"synthetase.":[85,240,303,403],"Incubation":[86],"either":[88],"resulted":[92,208],"in":[93,209,237,269,319,375,396,415],"rapid":[95],"loss":[96,212],"both":[98],"aminoacylation":[101],"isoleucinedependent":[103],"isotopic":[104,215],"ATP-PPi":[105],"exchange":[106,217],"activities.":[107],"stoichiometries":[109],"ADP-PL":[114,142],"or":[115,163,189,417],"tRNA(Ile)ox":[116],"corresponded":[117],"1":[119],"mol":[120,125],"incorporated":[123],"per":[124],"enzyme.":[127],"Altogether,":[128],"oxidized":[130],"moiety":[137],"analog":[141,257],"react":[143],"lysyl":[146],"residues":[147,253,307],"601":[148],"604":[150],"consensus":[153],"(601)KMSKS(605).":[155],"Identification":[156],"L-isoleucine":[162,187],"non":[165],"cognate":[166],"acids":[168,194],"achieved":[174],"qualitative":[176],"comparative":[177],"bromomethyl":[183],"ketone":[184],"derivatives":[185],"(IBMK)":[188],"non-cognate":[192],"valine":[195],"(VBMK),":[196],"phenylalanine":[197],"(FBMK)":[198],"norleucine":[200],"(NleBMK).":[201],"Labeling":[202],"enzyme":[205],"IBMK":[207,258],"complete":[211],"isoleucine-dependent":[214],"[(32)P]PPi-ATP":[216],"activity.":[218],"VBMK,":[219,264],"NleBMK":[220,265],"FBMK":[222,232,267],"were":[223,286],"also":[224],"capable":[225],"abolishing":[227],"activity":[229],"IleRS,":[231,262,383],"being":[233],"less":[235],"efficient":[236],"inactivating":[238],"Analysis":[241],"spectrometry":[245],"designated":[246],"cysteines-462":[247],"-718":[249],"as":[250],"target":[252],"substrate":[256,311],"labeled":[268,308],"common":[270],"His-394,":[271],"His-478":[272],"Cys-718.":[274],"In":[275,334],"addition,":[276],"VBMK":[277,293],"NleBMK,":[279],"which":[280],"are":[281,313,328,373],"chemically":[282],"similar":[283],"IBMK,":[285],"found":[287],"covalently":[288],"bound":[289],"Cys-462,":[291],"specifically":[295],"attached":[296],"His-332":[298,379,408],"(or":[299,368],"His-337)":[300],"mainly":[314],"distributed":[315],"between":[316],"three":[317],"regions":[318],"primary":[321],"structure":[322],"IleRS:":[326],"these":[327],"segments":[329],"[325-394],":[330],"[451-479]":[331,357],"[591-604].":[333],"3-D":[336],"structures":[337],"from":[340],"T.":[341],"thermophilus":[342],"S.":[344],"aureus,":[345],"[325-394]":[347],"stretch":[348],"is":[349,385,394,405],"part":[350],"editing":[353,398],"domain,":[354,371],"while":[355],"fragments":[356],"[591-604]":[359],"representing":[360],"isoleucine":[362],"domain":[364],"dinucleotide":[367],"Rossmann)":[369],"fold":[370],"respectively,":[372],"located":[374,395],"catalytic":[377],"core.":[378],"that":[384,407],"strictly":[386],"conserved":[387],"among":[388],"all":[389],"available":[391],"sequences":[393],"It":[404],"proposed":[406],"participates":[413],"directly":[414],"hydrolysis,":[416],"helps":[418],"deprotonate":[420],"hydroxyl":[422],"group":[423],"threonine":[425],"at":[426],"hydrolytic":[428],"site.":[429]},"counts_by_year":[{"year":2022,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}