{"id":"https://openalex.org/W2020708384","doi":"https://doi.org/10.1021/bi00408a042","title":"Substrate specificity of the 3-methylaspartate ammonia-lyase reaction: observation of differential relative reaction rates for substrate-product pairs","display_name":"Substrate specificity of the 3-methylaspartate ammonia-lyase reaction: observation of differential relative reaction rates for substrate-product pairs","publication_year":1988,"publication_date":"1988-04-19","ids":{"openalex":"https://openalex.org/W2020708384","doi":"https://doi.org/10.1021/bi00408a042","mag":"2020708384","pmid":"https://pubmed.ncbi.nlm.nih.gov/3401458"},"language":"en","primary_location":{"id":"doi:10.1021/bi00408a042","doi":"https://doi.org/10.1021/bi00408a042","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00408a042","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044273464","display_name":"Nigel P. Botting","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nigel P. Botting","raw_affiliation_strings":["Department of Chemistry, University , Southhampton, U.K"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University , Southhampton, U.K","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044925039","display_name":"M. Akhtar","orcid":"https://orcid.org/0000-0002-8805-0373","observed_orcids":["https://orcid.org/0000-0002-8805-0373","https://orcid.org/0000-0002-8610-2686"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mahmoud Akhtar","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046812199","display_name":"Mark A. Cohen","orcid":"https://orcid.org/0000-0002-1548-2773","observed_orcids":["https://orcid.org/0000-0002-1548-2773"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mark A. Cohen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5020699680","display_name":"David Gani","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"David Gani","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.2175,"has_fulltext":false,"cited_by_count":28,"citation_normalized_percentile":{"value":0.40592131,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"27","issue":"8","first_page":"2953","last_page":"2955"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.5356000065803528,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.5356000065803528,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.45019999146461487,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.925000011920929},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.8730000257492065},{"id":"https://openalex.org/keywords/deamination","display_name":"deamination","score":0.7039999961853027},{"id":"https://openalex.org/keywords/michaelis-constant","display_name":"Michaelis constant","score":0.7369999885559082},{"id":"https://openalex.org/keywords/e1cb-mechanism","display_name":"E1cB mechanism","score":0.8790000081062317},{"id":"https://openalex.org/keywords/c-n-bond-cleavage","display_name":"C\u2013N bond cleavage","score":0.906000018119812},{"id":"https://openalex.org/keywords/substituent-size-effects","display_name":"substituent size effects","score":0.515999972820282}],"concepts":[{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.6804611086845398},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.5930395126342773},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5569872260093689},{"id":"https://openalex.org/C90673727","wikidata":"https://www.wikidata.org/wiki/Q901718","display_name":"Product (mathematics)","level":2,"score":0.4550018310546875},{"id":"https://openalex.org/C23123220","wikidata":"https://www.wikidata.org/wiki/Q816826","display_name":"Information retrieval","level":1,"score":0.45417946577072144},{"id":"https://openalex.org/C93226319","wikidata":"https://www.wikidata.org/wiki/Q193137","display_name":"Differential (mechanical device)","level":2,"score":0.4432017207145691},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.3280106484889984},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3246240019798279},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.19314974546432495},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.18474826216697693},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.11070942878723145},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.10302978754043579},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.07865175604820251},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000642","descriptor_name":"Ammonia-Lyases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003013","descriptor_name":"Clostridium","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00408a042","doi":"https://doi.org/10.1021/bi00408a042","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00408a042","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:3401458","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3401458","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W1477916074","https://openalex.org/W1574132210","https://openalex.org/W1985255279","https://openalex.org/W2030790519","https://openalex.org/W2052962555","https://openalex.org/W2078532632","https://openalex.org/W2080974330","https://openalex.org/W2410946195","https://openalex.org/W2426481551","https://openalex.org/W2471864908"],"related_works":["https://openalex.org/W1981656434","https://openalex.org/W2168915580","https://openalex.org/W2044539576","https://openalex.org/W2073478341","https://openalex.org/W3026968444","https://openalex.org/W7030094561","https://openalex.org/W2058617726","https://openalex.org/W1990692674","https://openalex.org/W2903114524","https://openalex.org/W1985255279"],"abstract_inverted_index":{"A":[0],"range":[1],"of":[2,25,42,46,56,81,85,137,140,193,197,204],"substituted":[3],"fumaric":[4],"and":[5,19,34,105,143],"aspartic":[6,198],"acid":[7,92,199],"substrates":[8,50,76,93,200],"for":[9,44,74,87,107,112,156,165,212],"the":[10,23,26,31,47,54,75,88,110,127,135,138,141,162,174,194,202,205,209],"enzyme":[11,175],"3-methylaspartate":[12],"ammonia-lyase":[13],"(EC":[14],"4.3.1.2)":[15],"have":[16],"been":[17],"synthesized":[18],"used":[20],"to":[21,134,150,160],"study":[22],"kinetics":[24],"catalyzed":[27],"reaction":[28,38,118],"in":[29,126,152,158],"both":[30],"forward":[32],"(deamination)":[33],"reverse":[35],"(conjugative":[36],"amination)":[37],"directions.":[39],"The":[40,83],"rates":[41,84,119],"amination":[43],"all":[45],"alpha,":[48],"beta-unsaturated":[49],"studied":[51],"(bearing":[52],"substituents":[53],"size":[55,136],"an":[57,178],"ethyl":[58,151],"group":[59,148],"or":[60],"smaller)":[61],"were":[62],"similar":[63],"under":[64,98],"[s]":[65,99],"much":[66,100],"greater":[67,101],"than":[68,102],"KM":[69,72,103],"conditions":[70],"although":[71],"values":[73],"varied":[77,94],"by":[78],"a":[79,124,145],"factor":[80],"25.":[82],"deamination":[86,128],"corresponding":[89],"3-substituted":[90],"amino":[91],"widely":[95],"with":[96,208],"structure":[97],"conditions,":[104],"thus":[106],"substrate-product":[108],"pairs":[109],"ratio":[111],"V(forward)/V(reverse)":[113],"also":[114],"varied.":[115],"These":[116],"differential":[117],"indicate":[120],"that":[121,130,144,169,173,191],"there":[122],"is":[123,131,154,171,185,189],"step":[125],"direction":[129],"especially":[132],"sensitive":[133],"3-substituent":[139],"substrate":[142],"relatively":[146],"large":[147],"(methyl":[149],"size)":[153],"required":[155],"binding":[157,192],"order":[159],"reduce":[161],"activation":[163],"energy":[164],"this":[166],"step.":[167],"Given":[168],"it":[170,188],"proposed":[172],"operates":[176],"via":[177],"E1cb-type":[179],"mechanism":[180],"where":[181],"C-N":[182,210],"bond":[183,211],"cleavage":[184],"rate":[186],"limiting,":[187],"likely":[190],"C-3":[195],"substituent":[196],"affects":[201],"alignment":[203],"nascent":[206],"carbanion":[207],"elimination.":[213]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}