{"id":"https://openalex.org/W2020698198","doi":"https://doi.org/10.1104/pp.84.3.924","title":"Temperature-Induced Protein Conformational Changes in Barley Root Plasma Membrane-Enriched Microsomes","display_name":"Temperature-Induced Protein Conformational Changes in Barley Root Plasma Membrane-Enriched Microsomes","publication_year":1987,"publication_date":"1987-07-01","ids":{"openalex":"https://openalex.org/W2020698198","doi":"https://doi.org/10.1104/pp.84.3.924","mag":"2020698198","pmid":"https://pubmed.ncbi.nlm.nih.gov/16665545","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1056696"},"language":"en","primary_location":{"id":"doi:10.1104/pp.84.3.924","doi":"https://doi.org/10.1104/pp.84.3.924","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.84.3.924","pdf_url":"https://academic.oup.com/plphys/article-pdf/84/3/924/35633606/plphys_v84_3_924.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/plphys/article-pdf/84/3/924/35633606/plphys_v84_3_924.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111448455","display_name":"Charles R. Caldwell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1336096307","display_name":"United States Department of Agriculture","ror":"https://ror.org/01na82s61","country_code":"US","type":"government","lineage":["https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I4210125976","display_name":"Beltsville Agricultural Research Center","ror":"https://ror.org/03b08sh51","country_code":"US","type":"facility","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210125976","https://openalex.org/I4210138094"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Charles R. Caldwell","raw_affiliation_strings":["Plant Stress Laboratory, United States Department of Agriculture, Beltsville, Maryland 20705"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Plant Stress Laboratory, United States Department of Agriculture, Beltsville, Maryland 20705","institution_ids":["https://openalex.org/I1336096307","https://openalex.org/I4210125976"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5111448455"],"corresponding_institution_ids":["https://openalex.org/I1336096307","https://openalex.org/I4210125976"],"apc_list":{"value":3851,"currency":"USD","value_usd":3851},"apc_paid":null,"fwci":0.4641,"has_fulltext":true,"cited_by_count":14,"citation_normalized_percentile":{"value":0.55280483,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":93},"biblio":{"volume":"84","issue":"3","first_page":"924","last_page":"929"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.8324999809265137,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.8324999809265137,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.07190000265836716,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.03480000048875809,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-conformational-change","display_name":"protein conformational change","score":0.7820000052452087},{"id":"https://openalex.org/keywords/temperature-effects","display_name":"temperature effects","score":0.5170000195503235},{"id":"https://openalex.org/keywords/tryptophan-fluorescence","display_name":"tryptophan fluorescence","score":0.9829999804496765},{"id":"https://openalex.org/keywords/fluorescence-quenching","display_name":"fluorescence quenching","score":0.6819999814033508},{"id":"https://openalex.org/keywords/acrylamide","display_name":"acrylamide","score":0.7940000295639038},{"id":"https://openalex.org/keywords/potassium-iodide","display_name":"potassium iodide","score":0.9810000061988831},{"id":"https://openalex.org/keywords/glutaraldehyde","display_name":"glutaraldehyde","score":0.6290000081062317},{"id":"https://openalex.org/keywords/detergent-effects","display_name":"detergent effects","score":0.4350000023841858},{"id":"https://openalex.org/keywords/fluorescence-polarization","display_name":"fluorescence polarization","score":0.609000027179718},{"id":"https://openalex.org/keywords/plasma-membrane-atpase","display_name":"plasma membrane ATPase","score":0.8349999785423279},{"id":"https://openalex.org/keywords/membrane-protein-dynamics","display_name":"membrane protein dynamics","score":0.6000000238418579}],"concepts":[{"id":"https://openalex.org/C87644729","wikidata":"https://www.wikidata.org/wiki/Q547502","display_name":"Microsome","level":3,"score":0.722403883934021},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5539450645446777},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.5495119690895081},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5283318758010864},{"id":"https://openalex.org/C82706917","wikidata":"https://www.wikidata.org/wiki/Q10251","display_name":"Plasma","level":2,"score":0.5162703990936279},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4128805994987488},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24353542923927307},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.14582568407058716},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.07332167029380798},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1104/pp.84.3.924","doi":"https://doi.org/10.1104/pp.84.3.924","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.84.3.924","pdf_url":"https://academic.oup.com/plphys/article-pdf/84/3/924/35633606/plphys_v84_3_924.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1056696","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1056696","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC1056696/pdf/plntphys00616-0374.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiol","raw_type":"Text"},{"id":"pmid:16665545","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16665545","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant physiology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1104/pp.84.3.924","doi":"https://doi.org/10.1104/pp.84.3.924","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.84.3.924","pdf_url":"https://academic.oup.com/plphys/article-pdf/84/3/924/35633606/plphys_v84_3_924.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2020698198.pdf","grobid_xml":"https://content.openalex.org/works/W2020698198.grobid-xml"},"referenced_works_count":17,"referenced_works":["https://openalex.org/W72907974","https://openalex.org/W1577690292","https://openalex.org/W1954923312","https://openalex.org/W1979192073","https://openalex.org/W1989744185","https://openalex.org/W2001255467","https://openalex.org/W2001565018","https://openalex.org/W2013909009","https://openalex.org/W2045202073","https://openalex.org/W2053987038","https://openalex.org/W2061124406","https://openalex.org/W2076279650","https://openalex.org/W2078945859","https://openalex.org/W2079661361","https://openalex.org/W2081031303","https://openalex.org/W2087483937","https://openalex.org/W2092861677"],"related_works":["https://openalex.org/W1973667464","https://openalex.org/W1540116407","https://openalex.org/W2055419386","https://openalex.org/W178841642","https://openalex.org/W1999315010","https://openalex.org/W1937606011","https://openalex.org/W2329449799","https://openalex.org/W2060235564","https://openalex.org/W99051308","https://openalex.org/W4402037007"],"abstract_inverted_index":{"The":[0,21,32,102,133],"membrane-bound":[1],"proteins":[2,145],"of":[3,17,79,86,104,118,142,150,164],"barley":[4,166],"(Hordeum":[5],"vulgare":[6],"L.":[7],"cv":[8],"Conquest)":[9],"root":[10,167],"plasma":[11,168],"membrane-enriched":[12],"microsomes":[13],"displayed":[14],"fluorescence":[15,23,37,67,84,108],"typical":[16],"protein-associated":[18],"trytophan":[19],"residues.":[20],"protein":[22,36,60,64,94,107,124],"intensity":[24,38,85],"was":[25,39],"sensitive":[26],"to":[27,122,152],"variations":[28],"in":[29,35,92,116,139],"sample":[30],"temperature.":[31],"temperature-induced":[33],"decline":[34],"nonlinear":[40],"with":[41],"slope":[42],"discontinuities":[43],"at":[44,52,70,128],"about":[45,129],"12":[46,97,151],"and":[47,82,98,111],"32":[48,99,153],"degrees":[49,74,100,131,154],"C.":[50,75,101,132],"Detergents":[51],"levels":[53],"above":[54,72],"their":[55],"critical":[56],"micelle":[57],"concentration":[58],"enhanced":[59],"fluorescence.":[61,65],"Glutaraldehyde":[62],"reduced":[63],"Protein":[66],"polarization":[68],"increased":[69],"temperatures":[71],"30":[73],"Both":[76],"the":[77,83,87,105,119,123,140,143,147,160,165],"rate":[78],"tryptophan":[80,125],"photoionization":[81,88],"products":[89],"suggested":[90],"alterations":[91],"membrane":[93,144,169],"conformation":[95],"between":[96],"quenching":[103],"intrinsic":[106],"by":[109],"acrylamide":[110],"potassium":[112],"iodide":[113],"indicated":[114],"changes":[115,138],"accessibility":[117],"extrinsic":[120],"agents":[121],"residues":[126],"beginning":[127],"14":[130],"results":[134],"indicate":[135],"thermally":[136],"induced":[137],"dynamics":[141],"over":[146],"temperature":[148,162],"range":[149],"C":[155],"which":[156],"could":[157],"account":[158],"for":[159],"complex":[161],"dependence":[163],"ATPase.":[170]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2016,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}