{"id":"https://openalex.org/W2020366743","doi":"https://doi.org/10.1094/pdis-03-12-0305-pdn","title":"Identification and Characterization of Pale Potato Cyst Nematode (Globodera pallida) in Tepl\u00e1, the Czech Republic","display_name":"Identification and Characterization of Pale Potato Cyst Nematode (Globodera pallida) in Tepl\u00e1, the Czech Republic","publication_year":2012,"publication_date":"2012-05-16","ids":{"openalex":"https://openalex.org/W2020366743","doi":"https://doi.org/10.1094/pdis-03-12-0305-pdn","mag":"2020366743","pmid":"https://pubmed.ncbi.nlm.nih.gov/30727167"},"language":"en","primary_location":{"id":"doi:10.1094/pdis-03-12-0305-pdn","doi":"https://doi.org/10.1094/pdis-03-12-0305-pdn","is_oa":false,"landing_page_url":"https://doi.org/10.1094/pdis-03-12-0305-pdn","pdf_url":null,"source":{"id":"https://openalex.org/S135458494","display_name":"Plant Disease","issn_l":"0191-2917","issn":["0191-2917","1943-7692"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320276","host_organization_name":"American Phytopathological Society","host_organization_lineage":["https://openalex.org/P4310320276"],"host_organization_lineage_names":["American Phytopathological Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Disease","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005979551","display_name":"Ond\u0159ej Douda","orcid":"https://orcid.org/0000-0002-0808-2819","observed_orcids":["https://orcid.org/0000-0002-0808-2819"]},"institutions":[{"id":"https://openalex.org/I144280042","display_name":"Czech Agrifood Research Center","ror":"https://ror.org/0436mv865","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I144280042"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"O. Douda","raw_affiliation_strings":["Division of Plant Medicine, Crop Research Institute, 161 06 Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Plant Medicine, Crop Research Institute, 161 06 Prague, Czech Republic","institution_ids":["https://openalex.org/I144280042"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045043774","display_name":"Miloslav Zouhar","orcid":"https://orcid.org/0000-0003-4546-8005","observed_orcids":["https://orcid.org/0000-0003-4546-8005"]},"institutions":[{"id":"https://openalex.org/I205984670","display_name":"Czech University of Life Sciences Prague","ror":"https://ror.org/0415vcw02","country_code":"CZ","type":"education","lineage":["https://openalex.org/I205984670"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"M. Zouhar","raw_affiliation_strings":["Department of Plant Protection, Czech University of Agriculture, 165 21 Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Protection, Czech University of Agriculture, 165 21 Prague, Czech Republic","institution_ids":["https://openalex.org/I205984670"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101866459","display_name":"Jan Urban","orcid":"https://orcid.org/0000-0002-9924-9855","observed_orcids":["https://orcid.org/0000-0002-9924-9855"]},"institutions":[{"id":"https://openalex.org/I205984670","display_name":"Czech University of Life Sciences Prague","ror":"https://ror.org/0415vcw02","country_code":"CZ","type":"education","lineage":["https://openalex.org/I205984670"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"J. Urban","raw_affiliation_strings":["Department of Plant Protection, Czech University of Agriculture, 165 21 Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Protection, Czech University of Agriculture, 165 21 Prague, Czech Republic","institution_ids":["https://openalex.org/I205984670"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047922547","display_name":"V\u00e1clav \u010cerm\u00e1k","orcid":"https://orcid.org/0000-0001-7071-5755","observed_orcids":["https://orcid.org/0000-0001-7071-5755"]},"institutions":[{"id":"https://openalex.org/I4210092335","display_name":"State Phytosanitary Administration","ror":"https://ror.org/00ddcgv12","country_code":"CZ","type":"government","lineage":["https://openalex.org/I4210092335","https://openalex.org/I4210144479"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"V. \u010cerm\u00e1k","raw_affiliation_strings":["State Phytosanitary Administration of the Czech Republic, Department of Diagnostics, 779 00 Olomouc, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Phytosanitary Administration of the Czech Republic, Department of Diagnostics, 779 00 Olomouc, Czech Republic","institution_ids":["https://openalex.org/I4210092335"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088858923","display_name":"V. Gaar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210092335","display_name":"State Phytosanitary Administration","ror":"https://ror.org/00ddcgv12","country_code":"CZ","type":"government","lineage":["https://openalex.org/I4210092335","https://openalex.org/I4210144479"]},{"id":"https://openalex.org/I4210144479","display_name":"Ministry of Agriculture","ror":"https://ror.org/049wftm75","country_code":"CZ","type":"government","lineage":["https://openalex.org/I4210144479"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"V. Gaar","raw_affiliation_strings":["State Phytosanitary Administration of the Czech Republic, Department of Diagnostics, 1601 06 Prague. This work was supported by the Czech Ministry of Agriculture, project number MZE0002700604, and \u201cS\u201d project of the Czech Ministry of Education, Youth and Sport"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Phytosanitary Administration of the Czech Republic, Department of Diagnostics, 1601 06 Prague. This work was supported by the Czech Ministry of Agriculture, project number MZE0002700604, and \u201cS\u201d project of the Czech Ministry of Education, Youth and Sport","institution_ids":["https://openalex.org/I4210092335","https://openalex.org/I4210144479"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.3561,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.65663587,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"96","issue":"9","first_page":"1386","last_page":"1386"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11179","display_name":"Nematode management and characterization studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11179","display_name":"Nematode management and characterization studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11074","display_name":"Helminth infection and control","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13679","display_name":"Tardigrade Biology and Ecology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/globodera-pallida","display_name":"Globodera pallida","score":0.9729999899864197},{"id":"https://openalex.org/keywords/potato-cyst-nematode","display_name":"potato cyst nematode","score":0.9610000252723694},{"id":"https://openalex.org/keywords/globodera-rostochiensis","display_name":"Globodera rostochiensis","score":0.9399999976158142},{"id":"https://openalex.org/keywords/nematode-identification","display_name":"nematode identification","score":0.7990000247955322},{"id":"https://openalex.org/keywords/18s-rdna","display_name":"18S rDNA","score":0.7799999713897705},{"id":"https://openalex.org/keywords/internal-transcribed-spacer","display_name":"ITS region","score":0.6600000262260437},{"id":"https://openalex.org/keywords/quarantine-pests","display_name":"quarantine pests","score":0.43799999356269836},{"id":"https://openalex.org/keywords/potato","display_name":"potato","score":0.367000013589859},{"id":"https://openalex.org/keywords/czech-republic","display_name":"Czech Republic","score":0.8939999938011169},{"id":"https://openalex.org/keywords/soil-nematodes","display_name":"soil nematodes","score":0.4699999988079071},{"id":"https://openalex.org/keywords/first-record","display_name":"first record","score":0.30300000309944153},{"id":"https://openalex.org/keywords/dna-barcoding","display_name":"DNA barcoding","score":0.4230000078678131},{"id":"https://openalex.org/keywords/nematode-taxonomy","display_name":"nematode taxonomy","score":0.42800000309944153}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8895034790039062},{"id":"https://openalex.org/C2778930020","wikidata":"https://www.wikidata.org/wiki/Q3042435","display_name":"Potato cyst nematode","level":3,"score":0.8173611164093018},{"id":"https://openalex.org/C2778326351","wikidata":"https://www.wikidata.org/wiki/Q595152","display_name":"Globodera rostochiensis","level":3,"score":0.756150484085083},{"id":"https://openalex.org/C33511622","wikidata":"https://www.wikidata.org/wiki/Q1851434","display_name":"Morphometrics","level":2,"score":0.7395263910293579},{"id":"https://openalex.org/C2776387195","wikidata":"https://www.wikidata.org/wiki/Q3347032","display_name":"Globodera pallida","level":4,"score":0.5998095273971558},{"id":"https://openalex.org/C2780597408","wikidata":"https://www.wikidata.org/wiki/Q193211","display_name":"Cyst","level":2,"score":0.528078556060791},{"id":"https://openalex.org/C2776410716","wikidata":"https://www.wikidata.org/wiki/Q7629472","display_name":"Stylet","level":2,"score":0.44517454504966736},{"id":"https://openalex.org/C42972112","wikidata":"https://www.wikidata.org/wiki/Q170201","display_name":"Veterinary medicine","level":1,"score":0.437495619058609},{"id":"https://openalex.org/C125333598","wikidata":"https://www.wikidata.org/wiki/Q2355208","display_name":"Nematology","level":3,"score":0.43669337034225464},{"id":"https://openalex.org/C2778830712","wikidata":"https://www.wikidata.org/wiki/Q5185","display_name":"Nematode","level":2,"score":0.43559107184410095},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.39100050926208496},{"id":"https://openalex.org/C90856448","wikidata":"https://www.wikidata.org/wiki/Q431","display_name":"Zoology","level":1,"score":0.23389169573783875},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.22855260968208313},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.12948042154312134},{"id":"https://openalex.org/C2778761015","wikidata":"https://www.wikidata.org/wiki/Q134172","display_name":"Solanaceae","level":3,"score":0.08369079232215881},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C126838900","wikidata":"https://www.wikidata.org/wiki/Q77604","display_name":"Radiology","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1094/pdis-03-12-0305-pdn","doi":"https://doi.org/10.1094/pdis-03-12-0305-pdn","is_oa":false,"landing_page_url":"https://doi.org/10.1094/pdis-03-12-0305-pdn","pdf_url":null,"source":{"id":"https://openalex.org/S135458494","display_name":"Plant Disease","issn_l":"0191-2917","issn":["0191-2917","1943-7692"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320276","host_organization_name":"American Phytopathological Society","host_organization_lineage":["https://openalex.org/P4310320276"],"host_organization_lineage_names":["American Phytopathological Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Disease","raw_type":"journal-article"},{"id":"pmid:30727167","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30727167","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant disease","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9208999872207642,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2121115449","https://openalex.org/W2036603765","https://openalex.org/W2792475994","https://openalex.org/W1567045419","https://openalex.org/W2064650477","https://openalex.org/W2935999990","https://openalex.org/W2070138757","https://openalex.org/W3029623645","https://openalex.org/W1983417082","https://openalex.org/W2549899561"],"abstract_inverted_index":{"Potato":[0],"cyst":[1,18,65,138],"nematode":[2],"poses":[3],"a":[4,110],"significant":[5],"threat":[6],"to":[7,61,89,134,158,235,335],"potato":[8,17,111],"producers":[9],"in":[10,30,67,78,100,114,308,318,368,380,388,402],"the":[11,31,37,68,79,101,115,125,225,230,240,252,256,263,267,300,351,354,362,369,378,381,385],"Czech":[12,32,69,80,382],"Republic.":[13],"Both":[14],"species":[15],"of":[16,94,117,137,142,166,180,205,255,266,282,313,337,353,365,372,400],"nematodes":[19,66],"(Globodera":[20],"rostochiensis":[21,55],"and":[22,34,41,46,124,140,171,210,229,245,262,306,324,347,376,404],"G.":[23,54,72,95,131,338,366],"pallida)":[24],"are":[25],"listed":[26],"as":[27,130],"quarantine":[28],"pests":[29],"Republic":[33,81,383],"also":[35],"by":[36,64],"European":[38,40],"Union,":[39],"Mediterranean":[42],"Plant":[43,49,421],"Protection":[44,50],"Organization,":[45],"North":[47],"American":[48],"Organization.":[51],"To":[52,357],"date,":[53],"was":[56,74,97,208,219,274,286],"responsible":[57],"for":[58,276],"all":[59],"damage":[60],"potatoes":[62],"caused":[63],"Republic,":[70],"while":[71,174],"pallida":[73,96,132,339,367],"recorded":[75],"only":[76,377],"once":[77],"(2)":[82,416],"8":[83],"years":[84],"ago.":[85],"It":[86],"is":[87,361],"important":[88],"note":[90],"that":[91],"this":[92,360],"occurrence":[93],"not":[98],"located":[99,113],"free-cultivation":[102,370],"area.":[103],"In":[104],"July":[105],"2011,":[106],"soil":[107],"samples":[108,232],"from":[109,148,221,288,340],"field":[112],"area":[116,371],"Tepl\u00e1":[118],"(Karlovy":[119],"Vary":[120,374],"Region)":[121],"were":[122,128,233,295,316],"collected,":[123],"cysts":[126,223],"extracted":[127,220],"identified":[129],"according":[133],"microscopic":[135],"observation":[136],"fenestra":[139,152,157,170],"morphology":[141],"juveniles":[143],"(1).":[144],"Cyst":[145],"morphometrics":[146,178],"(means":[147,179],"10":[149],"cysts)":[150],"included:":[151],"diameter":[153],"21.2":[154],"\u03bcm,":[155,161,186,189,192],"distance":[156],"anus":[159,172,196],"56.8":[160],"Granek's":[162],"ratio":[163],"2.7,":[164],"number":[165],"cuticular":[167],"ridges":[168],"between":[169],"14;":[173],"second":[175,379],"stage":[176],"juvenile":[177,206],"13":[181],"specimens)":[182],"were:":[183],"L":[184],"466.7":[185],"stylet":[187,211],"24.2":[188],"tail":[190],"53.2":[191],"body":[193],"width":[194],"at":[195],"13.2,":[197],"h":[198],"28.6,":[199],"c":[200],"8.8,":[201],"c'":[202],"4.0.":[203],"Terminus":[204],"tails":[207],"rounded,":[209],"knobs":[212],"possessed":[213],"distinct":[214],"forward":[215],"projections.":[216],"Total":[217],"DNA":[218,231,271],"single":[222],"using":[224,299],"TriPure":[226],"reagent":[227],"(Roche),":[228],"used":[234,275],"amplify":[236],"cistron":[237],"rDNA":[238],"with":[239],"following":[241],"primers:":[242],"18S,":[243],"5'-TTGATTAGGTCCCTGCCCTTT-3',":[244],"21S,":[246],"5'-TTTCACTCGCCGTTACTAAGG-3'.":[247],"The":[248,292,311,326],"amplified":[249,287],"region":[250],"contains":[251],"3'":[253],"end":[254,265],"18S":[257],"gene,":[258],"ITS1,":[259],"5.8S,":[260],"ITS2,":[261],"5'":[264],"28S":[268],"gene.":[269],"Pfu":[270],"polymerase":[272],"(Fermentas)":[273,305],"accurate":[277],"amplification.":[278],"A":[279],"PCR":[280,293,302],"product":[281],"approximately":[283],"1.0":[284,328],"kb":[285,329],"three":[289],"individual":[290],"cysts.":[291],"amplicons":[294],"cloned":[296],"into":[297],"pJET1.2":[298],"CloneJET":[301],"Cloning":[303],"Kit":[304],"sequenced":[307],"both":[309],"directions.":[310],"sequences":[312,330,336],"representative":[314],"isolates":[315],"deposited":[317],"GenBank":[319],"(Accession":[320],"Nos.":[321,344],"JQ692592,":[322],"JQ692593,":[323],"JQ692594).":[325],"resultant":[327],"showed":[331],"99%":[332],"nucleotide":[333],"identity":[334],"Canada":[341],"(GenBank":[342],"Accession":[343],"GQ294522.1,":[345],"GQ355975.1,":[346],"GQ294523.1),":[348],"thus":[349],"confirming":[350],"results":[352],"morphological":[355],"analyses.":[356],"our":[358],"knowledge,":[359],"first":[363,386],"detection":[364],"Karlovy":[373],"Region,":[375],"since":[384],"report":[387],"2003":[389],"(2).":[390],"References:":[391],"(1)":[392],"M.":[393,417],"W.":[394],"Brzeski.":[395],"Page":[396],"237":[397],"in:":[398],"Nematodes":[399],"Tylenchina":[401],"Poland":[403],"Temperate":[405],"Europe.":[406],"Muzeum":[407],"I":[408],"Instytut":[409],"Zoologii":[410],"Polska":[411],"Akademia":[412],"Nauk,":[413],"Warszawa":[414],"1998.":[415],"Zouhar":[418],"et":[419],"al.":[420],"Disease,":[422],"87:98,":[423],"2003.":[424]},"counts_by_year":[{"year":2014,"cited_by_count":1}],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2016-06-24T00:00:00"}