{"id":"https://openalex.org/W2020085571","doi":"https://doi.org/10.1021/bi00344a004","title":"Isolation and partial characterization of genomic clones coding for a human pro-.alpha.1(II) collagen chain and demonstration of restriction fragment length polymorphism at the 3' end of the gene","display_name":"Isolation and partial characterization of genomic clones coding for a human pro-.alpha.1(II) collagen chain and demonstration of restriction fragment length polymorphism at the 3' end of the gene","publication_year":1985,"publication_date":"1985-11-01","ids":{"openalex":"https://openalex.org/W2020085571","doi":"https://doi.org/10.1021/bi00344a004","mag":"2020085571","pmid":"https://pubmed.ncbi.nlm.nih.gov/3002437"},"language":"en","primary_location":{"id":"doi:10.1021/bi00344a004","doi":"https://doi.org/10.1021/bi00344a004","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00344a004","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017889408","display_name":"Anne Marie Nunez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anne Marie Nunez","raw_affiliation_strings":["National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036346491","display_name":"Clair A. Francomano","orcid":"https://orcid.org/0000-0002-4032-7425","observed_orcids":["https://orcid.org/0000-0002-4032-7425"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Clair Francomano","raw_affiliation_strings":["National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053531825","display_name":"Marian F. Young","orcid":"https://orcid.org/0000-0003-0929-8854","observed_orcids":["https://orcid.org/0000-0003-0929-8854"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marian F. Young","raw_affiliation_strings":["National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113553363","display_name":"George R. Martin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"George R. Martin","raw_affiliation_strings":["National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5102398216","display_name":"Yoshihiko Yamada","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yoshihiko Yamada","raw_affiliation_strings":["National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1299303238"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":2.2568,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.88572456,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"24","issue":"23","first_page":"6343","last_page":"6348"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11310","display_name":"Connective tissue disorders research","score":0.6416000127792358,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11310","display_name":"Connective tissue disorders research","score":0.6416000127792358,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12085","display_name":"Collagen: Extraction and Characterization","score":0.3167000114917755,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10261","display_name":"Genetic Associations and Epidemiology","score":0.0052999998442828655,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/type-ii-procollagen","display_name":"type II procollagen","score":0.859000027179718},{"id":"https://openalex.org/keywords/genomic-clones","display_name":"genomic clones","score":0.7009999752044678},{"id":"https://openalex.org/keywords/restriction-fragment-length-polymorphism","display_name":"restriction fragment length polymorphism","score":0.953000009059906},{"id":"https://openalex.org/keywords/hindiii-polymorphism","display_name":"HindIII polymorphism","score":0.8040000200271606}],"concepts":[{"id":"https://openalex.org/C168007829","wikidata":"https://www.wikidata.org/wiki/Q857299","display_name":"Restriction fragment length polymorphism","level":4,"score":0.5366595983505249},{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.5018689632415771},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4527944326400757},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.4126993417739868},{"id":"https://openalex.org/C23123220","wikidata":"https://www.wikidata.org/wiki/Q816826","display_name":"Information retrieval","level":1,"score":0.3834291398525238},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.36537685990333557},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.36188387870788574},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3461053967475891},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.2977703809738159},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.25731250643730164},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.18101468682289124}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005796","descriptor_name":"Genes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007962","descriptor_name":"Leukocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009693","descriptor_name":"Nucleic Acid Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011110","descriptor_name":"Polymorphism, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011347","descriptor_name":"Procollagen","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D046911","descriptor_name":"Macromolecular Substances","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00344a004","doi":"https://doi.org/10.1021/bi00344a004","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00344a004","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:3002437","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3002437","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W1558516755","https://openalex.org/W1564677921","https://openalex.org/W1606921260","https://openalex.org/W1964241112","https://openalex.org/W1979001230","https://openalex.org/W1986534360","https://openalex.org/W1988250394","https://openalex.org/W1989934357","https://openalex.org/W1991555793","https://openalex.org/W2007396871","https://openalex.org/W2007618593","https://openalex.org/W2010463792","https://openalex.org/W2011836510","https://openalex.org/W2016739495","https://openalex.org/W2028934005","https://openalex.org/W2032343668","https://openalex.org/W2035913563","https://openalex.org/W2036823562","https://openalex.org/W2039174291","https://openalex.org/W2041168586","https://openalex.org/W2044592811","https://openalex.org/W2051802962","https://openalex.org/W2054684040","https://openalex.org/W2063450941","https://openalex.org/W2066160869","https://openalex.org/W2067231547","https://openalex.org/W2077205250","https://openalex.org/W2084106399","https://openalex.org/W2090238172","https://openalex.org/W2090885889","https://openalex.org/W2091477263","https://openalex.org/W2166713757"],"related_works":["https://openalex.org/W2166713757","https://openalex.org/W2113676927","https://openalex.org/W1497123701","https://openalex.org/W2044975498","https://openalex.org/W2041168586","https://openalex.org/W41751146","https://openalex.org/W1579073579","https://openalex.org/W1589462468","https://openalex.org/W2069758421","https://openalex.org/W1503335744"],"abstract_inverted_index":{"We":[0],"have":[1],"isolated":[2],"two":[3],"clones":[4],"containing":[5,39],"19":[6],"kilobases":[7],"(kb)":[8],"of":[9,46,65,70,81,83,118,127,139],"the":[10,43,59,62,75,79,87,90,99,110,125,136,140],"human":[11,22,88,100],"gene":[12],"coding":[13,41],"for":[14,42,109],"a":[15,36,106,128,131],"pro-alpha":[16],"1":[17],"(II)":[18],"collagen":[19,101],"chain":[20],"from":[21,121],"lambda":[23],"genomic":[24,119],"DNA":[25],"libraries.":[26],"A":[27,51],"3'":[28,137],"clone,":[29,53],"HC2A,":[30],"was":[31,55],"selected":[32],"by":[33,57],"cross-hybridization":[34],"with":[35,61],"cDNA":[37],"clone":[38,102],"sequences":[40],"carboxy":[44],"propeptide":[45,77],"chick":[47,91],"type":[48,92,112],"II":[49,93,113],"procollagen.":[50],"second":[52],"HC2B,":[54],"obtained":[56],"screening":[58],"library":[60],"5'":[63],"part":[64],"HC2A.":[66],"The":[67],"sequence":[68],"analysis":[69],"exon":[71],"3":[72],"corresponding":[73],"to":[74,105],"C":[76],"reveals":[78],"presence":[80,126],"stretches":[82],"conserved":[84],"nucleotides":[85],"between":[86],"and":[89,130],"procollagen":[94,114],"genes.":[95],"On":[96],"Northern":[97],"blots,":[98],"hybridizes":[103],"strongly":[104],"5.5-kb":[107],"RNA":[108],"rat":[111],"chain.":[115],"Finally,":[116],"studies":[117],"DNAs":[120],"normal":[122],"individuals":[123],"reveal":[124],"HindIII":[129],"BamHI":[132],"polymorphic":[133],"site":[134],"at":[135],"end":[138],"gene.":[141]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}