{"id":"https://openalex.org/W2020008237","doi":"https://doi.org/10.1097/00019606-199609000-00010","title":"Nonradioisotopic Detection and Typing of Human Papillomaviruses by Use of Polymerase Chain Reaction and Single-strand Conformation Polymorphism","display_name":"Nonradioisotopic Detection and Typing of Human Papillomaviruses by Use of Polymerase Chain Reaction and Single-strand Conformation Polymorphism","publication_year":1996,"publication_date":"1996-09-01","ids":{"openalex":"https://openalex.org/W2020008237","doi":"https://doi.org/10.1097/00019606-199609000-00010","mag":"2020008237","pmid":"https://pubmed.ncbi.nlm.nih.gov/8866235"},"language":"en","primary_location":{"id":"doi:10.1097/00019606-199609000-00010","doi":"https://doi.org/10.1097/00019606-199609000-00010","is_oa":false,"landing_page_url":"https://doi.org/10.1097/00019606-199609000-00010","pdf_url":null,"source":{"id":"https://openalex.org/S99097673","display_name":"Diagnostic Molecular Pathology","issn_l":"1052-9551","issn":["1052-9551"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","jufo-1"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Diagnostic Molecular Pathology","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2020008237"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082657664","display_name":"Ingeborg Zehbe","orcid":"https://orcid.org/0000-0001-8192-1837","observed_orcids":["https://orcid.org/0000-0001-8192-1837"]},"institutions":[{"id":"https://openalex.org/I1342360035","display_name":"Uppsala University Hospital","ror":"https://ror.org/01apvbh93","country_code":"SE","type":"healthcare","lineage":["https://openalex.org/I1342360035"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Ingeborg Zehbe","raw_affiliation_strings":["Department of Pathology, University Hospital, Uppsala, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University Hospital, Uppsala, Sweden","institution_ids":["https://openalex.org/I1342360035"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112201027","display_name":"Jan F. S\u00e4llstr\u00f6m","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jan F. S\u00e4llstr\u00f6m","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068627832","display_name":"Magnus Evander","orcid":"https://orcid.org/0000-0001-6034-4807","observed_orcids":["https://orcid.org/0000-0001-6034-4807"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Magnus Evander","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027747045","display_name":"Karin Edlund","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Karin Edlund","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009214126","display_name":"Eva Rylander","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Eva Rylander","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111433624","display_name":"G\u00f6ran Wadell","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G\u00f6ran Wadell","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5111959607","display_name":"Erik Wilander","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Erik Wilander","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.6631,"has_fulltext":false,"cited_by_count":24,"citation_normalized_percentile":{"value":0.88564036,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"5","issue":"3","first_page":"206","last_page":"213"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10146","display_name":"Cervical Cancer and HPV Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10146","display_name":"Cervical Cancer and HPV Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10428","display_name":"Reproductive tract infections research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/human-papillomavirus","display_name":"human papillomavirus","score":0.9980000257492065},{"id":"https://openalex.org/keywords/hpv-genotyping","display_name":"HPV genotyping","score":0.8199999928474426},{"id":"https://openalex.org/keywords/single-strand-conformation-polymorphism","display_name":"single-strand conformation polymorphism","score":0.9509999752044678},{"id":"https://openalex.org/keywords/pcr-based-typing","display_name":"PCR typing","score":0.5860000252723694},{"id":"https://openalex.org/keywords/cervical-intraepithelial-neoplasia","display_name":"cervical intraepithelial neoplasia","score":0.8999999761581421},{"id":"https://openalex.org/keywords/hpv-16","display_name":"HPV16","score":0.7979999780654907},{"id":"https://openalex.org/keywords/silver-staining","display_name":"silver staining","score":0.6520000100135803},{"id":"https://openalex.org/keywords/hpv-6","display_name":"HPV 6","score":0.4309999942779541},{"id":"https://openalex.org/keywords/hpv-11","display_name":"HPV 11","score":0.8820000290870667},{"id":"https://openalex.org/keywords/hpv-18","display_name":"HPV 18","score":0.7559999823570251},{"id":"https://openalex.org/keywords/hpv-31","display_name":"HPV 31","score":0.7360000014305115},{"id":"https://openalex.org/keywords/hpv-33","display_name":"HPV 33","score":0.9049999713897705},{"id":"https://openalex.org/keywords/hpv35","display_name":"HPV35","score":0.9800000190734863},{"id":"https://openalex.org/keywords/hpv-45","display_name":"HPV45","score":0.9670000076293945},{"id":"https://openalex.org/keywords/hpv-51","display_name":"HPV51","score":0.9850000143051147},{"id":"https://openalex.org/keywords/hpv52","display_name":"HPV52","score":0.9929999709129333},{"id":"https://openalex.org/keywords/hpv-56","display_name":"HPV 56","score":0.996999979019165},{"id":"https://openalex.org/keywords/hpv-58","display_name":"HPV58","score":1.0}],"concepts":[{"id":"https://openalex.org/C113324615","wikidata":"https://www.wikidata.org/wiki/Q472302","display_name":"Login","level":2,"score":0.7756739854812622},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6881936192512512},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.6238102316856384},{"id":"https://openalex.org/C108827166","wikidata":"https://www.wikidata.org/wiki/Q175975","display_name":"Internet privacy","level":1,"score":0.44826847314834595},{"id":"https://openalex.org/C169093310","wikidata":"https://www.wikidata.org/wiki/Q3702971","display_name":"Personally identifiable information","level":2,"score":0.4439474046230316},{"id":"https://openalex.org/C2779235478","wikidata":"https://www.wikidata.org/wiki/Q286576","display_name":"Register (sociolinguistics)","level":2,"score":0.4294191002845764},{"id":"https://openalex.org/C23123220","wikidata":"https://www.wikidata.org/wiki/Q816826","display_name":"Information retrieval","level":1,"score":0.3916245102882385},{"id":"https://openalex.org/C38652104","wikidata":"https://www.wikidata.org/wiki/Q3510521","display_name":"Computer security","level":1,"score":0.23165836930274963},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000293","descriptor_name":"Adolescent","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002578","descriptor_name":"Uterine Cervical Dysplasia","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D002583","descriptor_name":"Uterine Cervical Neoplasms","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013194","descriptor_name":"Staining and Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017403","descriptor_name":"In Situ Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018807","descriptor_name":"Polymorphism, Single-Stranded Conformational","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D027383","descriptor_name":"Papillomaviridae","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":true},{"descriptor_ui":"D027383","descriptor_name":"Papillomaviridae","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D030361","descriptor_name":"Papillomavirus Infections","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D030361","descriptor_name":"Papillomavirus Infections","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D030361","descriptor_name":"Papillomavirus Infections","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1097/00019606-199609000-00010","doi":"https://doi.org/10.1097/00019606-199609000-00010","is_oa":false,"landing_page_url":"https://doi.org/10.1097/00019606-199609000-00010","pdf_url":null,"source":{"id":"https://openalex.org/S99097673","display_name":"Diagnostic Molecular Pathology","issn_l":"1052-9551","issn":["1052-9551"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","jufo-1"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Diagnostic Molecular Pathology","raw_type":"journal-article"},{"id":"pmid:8866235","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8866235","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Diagnostic molecular pathology : the American journal of surgical pathology, part B","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9476000070571899}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W1514290344","https://openalex.org/W1722478811","https://openalex.org/W1849162682","https://openalex.org/W1911489264","https://openalex.org/W1969353948","https://openalex.org/W1976051908","https://openalex.org/W1996027687","https://openalex.org/W2025841318","https://openalex.org/W2029343098","https://openalex.org/W2031920668","https://openalex.org/W2033207270","https://openalex.org/W2037268137","https://openalex.org/W2049760838","https://openalex.org/W2050717506","https://openalex.org/W2076028837","https://openalex.org/W2091091922","https://openalex.org/W2129106917","https://openalex.org/W2131626832","https://openalex.org/W2168491219","https://openalex.org/W2170385036","https://openalex.org/W2398351382","https://openalex.org/W2525313791"],"related_works":["https://openalex.org/W50702875","https://openalex.org/W2398395139","https://openalex.org/W2051761477","https://openalex.org/W626856626","https://openalex.org/W2018016423","https://openalex.org/W2123816550","https://openalex.org/W2045432467","https://openalex.org/W2033097891","https://openalex.org/W2399091228","https://openalex.org/W2464114051"],"abstract_inverted_index":{"The":[0,18,167,195],"polymerase":[1],"chain":[2],"reaction":[3],"(PCR),":[4],"used":[5,85],"to":[6,23,48,92,155,192,197],"detect":[7,24,49],"human":[8],"papillomavirus":[9],"(HPV),":[10],"is":[11,28,184,190,199],"finding":[12],"increasing":[13],"applications":[14],"in":[15,164,174],"clinical":[16],"laboratories.":[17],"standard":[19,74],"method":[20],"of":[21,79,142],"analysis":[22],"amplified":[25,56],"PCR":[26,204],"products":[27],"ethidium":[29],"bromide":[30],"gel":[31],"electrophoresis":[32,61],"combined":[33,66],"with":[34,67,169,206],"labor":[35],"intensive":[36],"blot":[37,161,181,213],"hybridization.":[38,166,182,194,214],"In":[39],"this":[40],"study,":[41],"we":[42,84],"describe":[43],"single-strand":[44],"conformation":[45],"polymorphism":[46],"(SSCP)":[47],"and":[50,106,140,153,163,177,186],"genotype":[51],"simultaneously":[52],"general":[53],"primer":[54],"GP5+/GP6+":[55],"HPV":[57,82,86,94,127,138,157],"DNA":[58],"using":[59,211],"semiautomated":[60],"on":[62],"polyacrylamide":[63],"gels":[64],"(PAGE)":[65],"sensitive":[68],"silver":[69],"staining.":[70],"To":[71],"establish":[72],"a":[73],"for":[75,137,173,179],"the":[76,80,109,115,130],"band":[77,125],"patterns":[78],"various":[81],"types,":[83],"plasmid":[87],"DNA,":[88],"which":[89],"allowed":[90],"us":[91],"distinguish":[93],"6,":[95],"11,":[96],"16,":[97],"18,":[98],"31,":[99],"33,":[100],"35,":[101],"45,":[102],"51,":[103],"52,":[104],"56,":[105],"58,":[107],"covering":[108],"most":[110,131],"frequently":[111],"recognized":[112],"types.":[113],"All":[114],"types":[116],"tested":[117],"are":[118],"separated":[119],"from":[120],"each":[121],"other,":[122],"demonstrating":[123],"diverse":[124],"patterns,":[126],"16":[128],"being":[129],"distinct.":[132],"We":[133,215],"also":[134],"investigated":[135],"PCR-SSCP":[136],"detection":[139],"typing":[141,226],"86":[143],"cervical":[144,148],"biopsies":[145],"diagnosed":[146],"as":[147],"intraepithelial":[149],"neoplasia":[150],"(CIN)":[151],"I-III":[152],"known":[154],"be":[156,220],"positive":[158],"by":[159],"PCR-slot":[160,180],"hybridization":[162,176],"situ":[165,175],"correlation":[168],"SSCP":[170,183,198,218],"was":[171],"91%":[172],"98%":[178],"reproducible":[185],"specific.":[187],"Its":[188],"sensitivity":[189],"comparable":[191],"slot-blot":[193],"interval":[196],"approximately":[200],"2":[201],"h":[202],"after":[203],"compared":[205],"several":[207],"days'":[208],"work":[209],"when":[210],"conventional":[212],"concluded":[216],"that":[217],"may":[219],"more":[221],"advantageous":[222],"than":[223],"other":[224],"PCR-based":[225],"technologies.":[227]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}