{"id":"https://openalex.org/W2019821615","doi":"https://doi.org/10.1002/rcm.2874","title":"A potential pitfall in 18 O\u2010based N\u2010linked glycosylation site mapping","display_name":"A potential pitfall in 18 O\u2010based N\u2010linked glycosylation site mapping","publication_year":2007,"publication_date":"2007-02-06","ids":{"openalex":"https://openalex.org/W2019821615","doi":"https://doi.org/10.1002/rcm.2874","mag":"2019821615","pmid":"https://pubmed.ncbi.nlm.nih.gov/17279607"},"language":"en","primary_location":{"id":"doi:10.1002/rcm.2874","doi":"https://doi.org/10.1002/rcm.2874","is_oa":false,"landing_page_url":"https://doi.org/10.1002/rcm.2874","pdf_url":null,"source":{"id":"https://openalex.org/S89689683","display_name":"Rapid Communications in Mass Spectrometry","issn_l":"0951-4198","issn":["0951-4198","1097-0231"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rapid Communications in Mass Spectrometry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5037316485","display_name":"Peggi M. Angel","orcid":"https://orcid.org/0000-0002-4436-555X","observed_orcids":["https://orcid.org/0000-0002-4436-555X"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peggi M. Angel","raw_affiliation_strings":["Complex Carbohydrate Research Center and the Departments of Biochemistry and Molecular Biology and Chemistry, University of Georgia, 315 Riverbend Road, Athens, GA 30302-4712, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Complex Carbohydrate Research Center and the Departments of Biochemistry and Molecular Biology and Chemistry, University of Georgia, 315 Riverbend Road, Athens, GA 30302-4712, USA","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085947482","display_name":"Jae\u2010Min Lim","orcid":"https://orcid.org/0000-0002-7153-0194","observed_orcids":["https://orcid.org/0000-0002-7153-0194"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jae\u2010Min Lim","raw_affiliation_strings":["Complex Carbohydrate Research Center and the Departments of Biochemistry and Molecular Biology and Chemistry, University of Georgia, 315 Riverbend Road, Athens, GA 30302-4712, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Complex Carbohydrate Research Center and the Departments of Biochemistry and Molecular Biology and Chemistry, University of Georgia, 315 Riverbend Road, Athens, GA 30302-4712, USA","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047989158","display_name":"Lance Wells","orcid":"https://orcid.org/0000-0003-4956-5363","observed_orcids":["https://orcid.org/0000-0003-4956-5363"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lance Wells","raw_affiliation_strings":["Complex Carbohydrate Research Center and the Departments of Biochemistry and Molecular Biology and Chemistry, University of Georgia, 315 Riverbend Road, Athens, GA 30302-4712, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Complex Carbohydrate Research Center and the Departments of Biochemistry and Molecular Biology and Chemistry, University of Georgia, 315 Riverbend Road, Athens, GA 30302-4712, USA","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028553584","display_name":"Carl Bergmann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Carl Bergmann","raw_affiliation_strings":["Complex Carbohydrate Research Center and the Departments of Biochemistry and Molecular Biology and Chemistry, University of Georgia, 315 Riverbend Road, Athens, GA 30302-4712, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Complex Carbohydrate Research Center and the Departments of Biochemistry and Molecular Biology and Chemistry, University of Georgia, 315 Riverbend Road, Athens, GA 30302-4712, USA","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072546813","display_name":"Ron Orlando","orcid":"https://orcid.org/0000-0002-4111-8326","observed_orcids":["https://orcid.org/0000-0002-4111-8326"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ron Orlando","raw_affiliation_strings":["Complex Carbohydrate Research Center and the Departments of Biochemistry and Molecular Biology and Chemistry, University of Georgia, 315 Riverbend Road, Athens, GA 30302-4712, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Complex Carbohydrate Research Center and the Departments of Biochemistry and Molecular Biology and Chemistry, University of Georgia, 315 Riverbend Road, Athens, GA 30302-4712, USA","institution_ids":["https://openalex.org/I165733156"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5072546813"],"corresponding_institution_ids":["https://openalex.org/I165733156"],"apc_list":{"value":4840,"currency":"USD","value_usd":4840},"apc_paid":null,"fwci":2.1178,"has_fulltext":false,"cited_by_count":59,"citation_normalized_percentile":{"value":0.86509549,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"21","issue":"5","first_page":"674","last_page":"682"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/18o-labeling","display_name":"18O labeling","score":0.7910000085830688},{"id":"https://openalex.org/keywords/pngase-f-deglycosylation","display_name":"PNGase F deglycosylation","score":0.8730000257492065},{"id":"https://openalex.org/keywords/trypsin-activity","display_name":"trypsin activity","score":0.718999981880188},{"id":"https://openalex.org/keywords/glycoproteins","display_name":"glycoproteins","score":0.6620000004768372}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9534751176834106},{"id":"https://openalex.org/C2777313579","wikidata":"https://www.wikidata.org/wiki/Q898365","display_name":"Glycosylation","level":2,"score":0.7744491100311279},{"id":"https://openalex.org/C2776817266","wikidata":"https://www.wikidata.org/wiki/Q83141460","display_name":"PNGase F","level":4,"score":0.5943372249603271},{"id":"https://openalex.org/C2775895851","wikidata":"https://www.wikidata.org/wiki/Q185906","display_name":"Asparagine","level":3,"score":0.588129997253418},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.568335771560669},{"id":"https://openalex.org/C108625454","wikidata":"https://www.wikidata.org/wiki/Q187126","display_name":"Glycoprotein","level":2,"score":0.5227418541908264},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.49986791610717773},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.48494771122932434},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.48297181725502014},{"id":"https://openalex.org/C2781338088","wikidata":"https://www.wikidata.org/wiki/Q903495","display_name":"Residue (chemistry)","level":2,"score":0.4791271388530731},{"id":"https://openalex.org/C30324644","wikidata":"https://www.wikidata.org/wiki/Q3334151","display_name":"N-linked glycosylation","level":4,"score":0.4378420412540436},{"id":"https://openalex.org/C31827203","wikidata":"https://www.wikidata.org/wiki/Q874296","display_name":"Tandem mass spectrometry","level":3,"score":0.43602603673934937},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.43010222911834717},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.41444259881973267},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.24159368872642517},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1349399983882904},{"id":"https://openalex.org/C206212055","wikidata":"https://www.wikidata.org/wiki/Q2553138","display_name":"Glycan","level":3,"score":0.07299995422363281}],"mesh":[{"descriptor_ui":"D000509","descriptor_name":"alpha-Fetoproteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000509","descriptor_name":"alpha-Fetoproteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001216","descriptor_name":"Asparagine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001216","descriptor_name":"Asparagine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001224","descriptor_name":"Aspartic Acid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001224","descriptor_name":"Aspartic Acid","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010103","descriptor_name":"Oxygen Isotopes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010103","descriptor_name":"Oxygen Isotopes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010449","descriptor_name":"Peptide Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011499","descriptor_name":"Protein Processing, Post-Translational","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D021241","descriptor_name":"Spectrometry, Mass, Electrospray Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053719","descriptor_name":"Tandem Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/rcm.2874","doi":"https://doi.org/10.1002/rcm.2874","is_oa":false,"landing_page_url":"https://doi.org/10.1002/rcm.2874","pdf_url":null,"source":{"id":"https://openalex.org/S89689683","display_name":"Rapid Communications in Mass Spectrometry","issn_l":"0951-4198","issn":["0951-4198","1097-0231"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rapid Communications in Mass Spectrometry","raw_type":"journal-article"},{"id":"pmid:17279607","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17279607","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rapid communications in mass spectrometry : RCM","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5662941156","display_name":"Resource for Integrated Glycotechnology","funder_award_id":"2p41rr005351-21","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G7794544523","display_name":"Integrated Technology Resource for Biomedial Glycomics","funder_award_id":"P41RR018502","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G791517991","display_name":null,"funder_award_id":"P41-RR005351","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W1970908974","https://openalex.org/W1975036946","https://openalex.org/W1985600844","https://openalex.org/W2007174430","https://openalex.org/W2008471565","https://openalex.org/W2016707540","https://openalex.org/W2019848394","https://openalex.org/W2022773191","https://openalex.org/W2024141877","https://openalex.org/W2026465178","https://openalex.org/W2028062152","https://openalex.org/W2031119708","https://openalex.org/W2033519877","https://openalex.org/W2048385302","https://openalex.org/W2054747743","https://openalex.org/W2059905961","https://openalex.org/W2061372590","https://openalex.org/W2065511115","https://openalex.org/W2080584760","https://openalex.org/W2080883962","https://openalex.org/W2084590373","https://openalex.org/W2091101858","https://openalex.org/W2099560426","https://openalex.org/W2100701404","https://openalex.org/W2101961263","https://openalex.org/W2105869274","https://openalex.org/W2123960992","https://openalex.org/W2142342668","https://openalex.org/W2158104865"],"related_works":["https://openalex.org/W2020392977","https://openalex.org/W1979080634","https://openalex.org/W4229019858","https://openalex.org/W1975036946","https://openalex.org/W2590976366","https://openalex.org/W2001583492","https://openalex.org/W2080883962","https://openalex.org/W1975565538","https://openalex.org/W4317618857","https://openalex.org/W2020683762"],"abstract_inverted_index":{"A":[0],"common":[1],"procedure":[2],"for":[3,86,118,145],"identifying":[4],"N-linked":[5,61,87],"glycosylation":[6],"sites":[7,59],"involves":[8],"tryptic":[9],"digestion":[10,29],"of":[11,18,60,71,81,99,128,133,147,167,228,243],"the":[12,16,58,72,82,97,115,126,131,134,137,148,153,165,179,184,189,203,210,226,244,254],"glycoprotein,":[13],"followed":[14],"by":[15,26,39,45,175,187,198],"conversion":[17],"glycosylated":[19,77],"asparagine":[20],"residues":[21],"into":[22,96,130,202],"(18)O-labeled":[23,161],"aspartic":[24],"acids":[25],"PNGase":[27],"F":[28],"in":[30,191,253],"(18)O":[31,129,200],"water.":[32],"The":[33,140],"3":[34],"Da":[35],"mass":[36,48,142],"tag":[37],"created":[38],"this":[40,65,100,257,267],"process":[41],"is":[42,53],"readily":[43],"observable":[44],"liquid":[46],"chromatography/tandem":[47],"spectrometry":[49],"(LC/MS/MS)":[50],"analysis,":[51],"and":[52,89,112,123,222,259,262],"often":[54],"used":[55,117],"to":[56,125,182,225,240,256,265],"identify":[57],"glycosylation.":[62],"While":[63],"using":[64],"procedure,":[66],"we":[67],"noticed":[68],"that":[69,107,230],"60%":[70],"asparagines":[73],"identified":[74],"as":[75,152,205],"being":[76],"were":[78,91],"not":[79,92],"part":[80],"consensus":[83],"sequence":[84],"required":[85],"glycosylation,":[88],"thus":[90],"biologically":[93],"possible.":[94],"Investigation":[95],"source":[98],"unacceptably":[101],"high":[102],"false":[103,149],"positive":[104,150],"rate":[105],"demonstrated":[106,217],"even":[108],"after":[109],"reversed-phase":[110],"cleanup":[111],"heat":[113],"denaturation,":[114],"trypsin":[116,177,234],"proteolysis":[119],"was":[120],"still":[121],"active":[122],"led":[124],"incorporation":[127,201],"C-termini":[132],"peptides":[135,190],"during":[136,209],"deglycosylation":[138,185],"step.":[139],"resulting":[141],"shift":[143],"accounted":[144],"most":[146],"sites,":[151],"database":[154,211],"search":[155],"algorithm":[156],"confused":[157],"it":[158],"with":[159,233],"an":[160],"Asp":[162],"residue":[163],"near":[164],"C-terminus":[166,204],"a":[168,206,219],"peptide.":[169],"This":[170,248],"problem":[171],"can":[172],"be":[173],"overcome":[174,266],"eliminating":[176],"from":[178],"solution":[180],"prior":[181,239],"performing":[183],"process,":[186],"resuspending":[188],"natural":[192],"abundance":[193],"water":[194],"following":[195],"deglycosylation,":[196],"or":[197,235],"allowing":[199],"variable":[207],"modification":[208],"search.":[212],"These":[213],"methods":[214],"have":[215],"been":[216],"on":[218],"model":[220],"protein,":[221],"are":[223,231],"applicable":[224],"analyses":[227],"glycoproteins":[229],"digested":[232],"another":[236],"serine":[237],"protease":[238],"enzymatic":[241],"release":[242],"carbohydrate":[245],"side":[246],"chains.":[247],"study":[249],"should":[250],"alert":[251],"investigators":[252],"field":[255],"potential":[258],"unexpected":[260],"pitfall":[261],"provide":[263],"strategies":[264],"phenomenon.":[268]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}