{"id":"https://openalex.org/W2019280464","doi":"https://doi.org/10.1074/jbc.m000279200","title":"Vertical-scanning Mutagenesis of a Critical Tryptophan in the \u201cMinor Groove Binding Track\u201d of HIV-1 Reverse Transcriptase","display_name":"Vertical-scanning Mutagenesis of a Critical Tryptophan in the \u201cMinor Groove Binding Track\u201d of HIV-1 Reverse Transcriptase","publication_year":2000,"publication_date":"2000-05-01","ids":{"openalex":"https://openalex.org/W2019280464","doi":"https://doi.org/10.1074/jbc.m000279200","mag":"2019280464","pmid":"https://pubmed.ncbi.nlm.nih.gov/10747890"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m000279200","doi":"https://doi.org/10.1074/jbc.m000279200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m000279200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.m000279200","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5072736112","display_name":"Gary J. Latham","orcid":"https://orcid.org/0000-0002-9263-3475","observed_orcids":["https://orcid.org/0000-0002-9263-3475"]},"institutions":[{"id":"https://openalex.org/I55302922","display_name":"The University of Texas Medical Branch at Galveston","ror":"https://ror.org/016tfm930","country_code":"US","type":"education","lineage":["https://openalex.org/I55302922"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gary J. Latham","raw_affiliation_strings":["Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1071, USA","Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1071"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1071, USA","institution_ids":["https://openalex.org/I55302922"]},{"raw_affiliation_string":"Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1071","institution_ids":["https://openalex.org/I55302922"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Eva Forgacs","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55302922","display_name":"The University of Texas Medical Branch at Galveston","ror":"https://ror.org/016tfm930","country_code":"US","type":"education","lineage":["https://openalex.org/I55302922"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eva Forgacs","raw_affiliation_strings":["Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1071"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1071","institution_ids":["https://openalex.org/I55302922"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062571628","display_name":"William A. Beard","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210095140","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William A. Beard","raw_affiliation_strings":["Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089715804","display_name":"Rajendra Prasad","orcid":"https://orcid.org/0000-0002-1627-6699","observed_orcids":["https://orcid.org/0000-0002-1627-6699"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210095140","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rajendra Prasad","raw_affiliation_strings":["Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088985122","display_name":"Katarzyna B\u0119benek","orcid":"https://orcid.org/0000-0002-3263-7992","observed_orcids":["https://orcid.org/0000-0002-3263-7992"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210095140","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Katarzyna Bebenek","raw_affiliation_strings":["Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054055369","display_name":"Thomas A. Kunkel","orcid":"https://orcid.org/0000-0002-9900-1788","observed_orcids":["https://orcid.org/0000-0002-9900-1788"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210095140","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas A. Kunkel","raw_affiliation_strings":["Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709","Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210095140"]},{"raw_affiliation_string":"Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045523035","display_name":"Samuel H. Wilson","orcid":"https://orcid.org/0000-0002-1702-5293","observed_orcids":["https://orcid.org/0000-0002-1702-5293"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210095140","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Samuel H. Wilson","raw_affiliation_strings":["Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055892592","display_name":"R. Stephen Lloyd","orcid":"https://orcid.org/0000-0001-7273-372X","observed_orcids":["https://orcid.org/0000-0001-7273-372X"]},"institutions":[{"id":"https://openalex.org/I55302922","display_name":"The University of Texas Medical Branch at Galveston","ror":"https://ror.org/016tfm930","country_code":"US","type":"education","lineage":["https://openalex.org/I55302922"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"R.Stephen Lloyd","raw_affiliation_strings":["Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1071"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1071","institution_ids":["https://openalex.org/I55302922"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5055892592"],"corresponding_institution_ids":["https://openalex.org/I55302922"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.8623,"has_fulltext":false,"cited_by_count":32,"citation_normalized_percentile":{"value":0.90083799,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"275","issue":"20","first_page":"15025","last_page":"15033"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10526","display_name":"HIV/AIDS drug development and treatment","score":0.6161999702453613,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10526","display_name":"HIV/AIDS drug development and treatment","score":0.6161999702453613,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.27219998836517334,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.03099999949336052,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hiv-1-reverse-transcriptase","display_name":"HIV-1 reverse transcriptase","score":1.0},{"id":"https://openalex.org/keywords/primer-extension","display_name":"primer extension","score":0.5019999742507935},{"id":"https://openalex.org/keywords/processivity","display_name":"processivity","score":0.6359999775886536},{"id":"https://openalex.org/keywords/major-groove","display_name":"major groove","score":0.6539999842643738},{"id":"https://openalex.org/keywords/protein-dna-interactions","display_name":"protein-DNA interactions","score":0.6320000290870667}],"concepts":[{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6363528966903687},{"id":"https://openalex.org/C185581394","wikidata":"https://www.wikidata.org/wiki/Q145911","display_name":"Base pair","level":3,"score":0.6109205484390259},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5920524001121521},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.5429984331130981},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.5315859913825989},{"id":"https://openalex.org/C2780227090","wikidata":"https://www.wikidata.org/wiki/Q2636892","display_name":"Alanine scanning","level":5,"score":0.48419931530952454},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.4841499924659729},{"id":"https://openalex.org/C194857396","wikidata":"https://www.wikidata.org/wiki/Q382878","display_name":"Primer extension","level":4,"score":0.4699813425540924},{"id":"https://openalex.org/C2776858809","wikidata":"https://www.wikidata.org/wiki/Q171973","display_name":"Thymine","level":3,"score":0.4565553069114685},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44062840938568115},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.39262357354164124},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3869297206401825},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3578900694847107},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.34427571296691895},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.14066848158836365},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11404138803482056},{"id":"https://openalex.org/C3017666073","wikidata":"https://www.wikidata.org/wiki/Q1764062","display_name":"Base sequence","level":3,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000225","descriptor_name":"Adenine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000409","descriptor_name":"Alanine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004852","descriptor_name":"Epoxy Compounds","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005998","descriptor_name":"Glycine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013237","descriptor_name":"Stereoisomerism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013698","descriptor_name":"Templates, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014364","descriptor_name":"Tryptophan","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015497","descriptor_name":"HIV-1","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016384","descriptor_name":"Consensus Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019943","descriptor_name":"Amino Acid Substitution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054303","descriptor_name":"HIV Reverse Transcriptase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D054303","descriptor_name":"HIV Reverse Transcriptase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D054303","descriptor_name":"HIV Reverse Transcriptase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m000279200","doi":"https://doi.org/10.1074/jbc.m000279200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m000279200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:10747890","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10747890","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m000279200","doi":"https://doi.org/10.1074/jbc.m000279200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m000279200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.7099999785423279}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2810292926","display_name":null,"funder_award_id":"ES 06492","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"},{"id":"https://openalex.org/G3352031668","display_name":"DNA ALKYLATION REGULATES HUMAN REPAIR GENES","funder_award_id":"5r01es006492-04","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G3434171862","display_name":null,"funder_award_id":"ES-06766","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"},{"id":"https://openalex.org/G581176990","display_name":"TNF-alpha to TGF-beta Signal Transduction","funder_award_id":"R01ES006766","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"},{"id":"https://openalex.org/G7928200420","display_name":"Chemistry and Biology of Carcinogen-DNA Adducts","funder_award_id":"P01ES005355","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"},{"id":"https://openalex.org/G842292894","display_name":null,"funder_award_id":"ES 05355","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337361","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":37,"referenced_works":["https://openalex.org/W1481873107","https://openalex.org/W1493170168","https://openalex.org/W1504602313","https://openalex.org/W1534084062","https://openalex.org/W1557097517","https://openalex.org/W1939421214","https://openalex.org/W1971640816","https://openalex.org/W1973711348","https://openalex.org/W1988607615","https://openalex.org/W1988840026","https://openalex.org/W1996918171","https://openalex.org/W2005610074","https://openalex.org/W2008954911","https://openalex.org/W2009103687","https://openalex.org/W2009581957","https://openalex.org/W2012290180","https://openalex.org/W2013544902","https://openalex.org/W2016721288","https://openalex.org/W2018542294","https://openalex.org/W2018809187","https://openalex.org/W2024782634","https://openalex.org/W2036383888","https://openalex.org/W2041249795","https://openalex.org/W2065740427","https://openalex.org/W2069815958","https://openalex.org/W2073873696","https://openalex.org/W2086624754","https://openalex.org/W2090126383","https://openalex.org/W2092786021","https://openalex.org/W2092921193","https://openalex.org/W2105267696","https://openalex.org/W2126644478","https://openalex.org/W2130233138","https://openalex.org/W2149410625","https://openalex.org/W2952371361","https://openalex.org/W4252237752","https://openalex.org/W4298363274"],"related_works":["https://openalex.org/W2013544902","https://openalex.org/W2081263792","https://openalex.org/W2024782634","https://openalex.org/W1996918171","https://openalex.org/W2082990246","https://openalex.org/W1627951033","https://openalex.org/W2012902539","https://openalex.org/W1971640816","https://openalex.org/W2063347630","https://openalex.org/W2073873696"],"abstract_inverted_index":{"Biochemical":[0],"and":[1,33,74,148,168,203],"molecular":[2,86],"modeling":[3],"studies":[4],"of":[5,76,95,131,152,163,183,198],"human":[6],"immunodeficiency":[7],"virus":[8],"type":[9],"1":[10,167],"reverse":[11],"transcriptase":[12],"(RT)":[13],"have":[14],"revealed":[15],"that":[16,72,190,234],"a":[17,59,123,195],"structural":[18,60,223],"element,":[19],"the":[20,40,45,52,56,77,85,117,145,161,175,180,184,216,226,239],"minor":[21,42,200,231,240],"groove":[22,43,182,201,218,232],"binding":[23],"track":[24],"(MGBT),":[25],"is":[26],"important":[27,80,222],"for":[28,105],"both":[29],"replication":[30],"frameshift":[31],"fidelity":[32],"processivity.":[34],"The":[35],"MGBT":[36,78],"interactions":[37,87,202,208],"occur":[38],"in":[39,89,99,111,115,179,225],"DNA":[41,57,81,119,139],"from":[44,51,62],"second":[46],"through":[47],"sixth":[48],"base":[49,206],"pair":[50,207],"primer":[53,112],"3'-terminus":[54],"where":[55],"undergoes":[58],"transition":[61],"A-like":[63],"to":[64,137,238],"B-form":[65],"DNA.":[66],"Alanine-scanning":[67],"mutagenesis":[68],"had":[69],"previously":[70],"demonstrated":[71],"Gly(262)":[73,153],"Trp(266)":[75,146],"contributes":[79],"interactions.":[82],"To":[83],"probe":[84],"occurring":[88],"this":[90],"critical":[91],"region,":[92],"eight":[93],"mutants":[94,147],"RT":[96,236],"were":[97,103,109],"studied":[98],"which":[100,116,205],"alternate":[101],"residues":[102],"substituted":[104],"Trp(266).":[106],"These":[107,134,187],"enzymes":[108],"characterized":[110],"extension":[113],"assays":[114],"template":[118],"was":[120,177],"adducted":[121],"at":[122],"single":[124],"adenine":[125],"by":[126,141,211],"either":[127],"R-":[128],"or":[129],"S-enantiomers":[130],"styrene":[132,157],"oxide.":[133],"lesions":[135],"failed":[136],"block":[138],"polymerization":[140],"wild-type":[142],"RT,":[143],"yet":[144],"an":[149],"alanine":[150],"mutant":[151],"terminated":[154],"synthesis":[155],"on":[156],"oxide-adducted":[158],"templates.":[159],"Significantly,":[160],"sites":[162],"termination":[164],"occurred":[165],"primarily":[166],"3":[169],"bases":[170],"following":[171],"adduct":[172],"bypass,":[173],"when":[174],"lesion":[176,219],"positioned":[178],"major":[181,217],"template-primer":[185,227],"stem.":[186],"results":[188],"indicate":[189],"residue":[191],"266":[192],"serves":[193],"as":[194,230],"\"protein":[196],"sensor\"":[197],"altered":[199,210],"identifies":[204],"are":[209],"these":[212],"lesions.":[213],"In":[214],"addition,":[215],"must":[220],"alter":[221],"transitions":[224],"stem,":[228],"such":[229],"widening,":[233],"allow":[235],"access":[237],"groove.":[241]},"counts_by_year":[{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}