{"id":"https://openalex.org/W2018925860","doi":"https://doi.org/10.1002/jnr.490170314","title":"Cyclic nucleotide phosphodiesterase isozymes in neuroblastoma cells","display_name":"Cyclic nucleotide phosphodiesterase isozymes in neuroblastoma cells","publication_year":1987,"publication_date":"1987-01-01","ids":{"openalex":"https://openalex.org/W2018925860","doi":"https://doi.org/10.1002/jnr.490170314","mag":"2018925860","pmid":"https://pubmed.ncbi.nlm.nih.gov/3037096"},"language":"en","primary_location":{"id":"doi:10.1002/jnr.490170314","doi":"https://doi.org/10.1002/jnr.490170314","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jnr.490170314","pdf_url":null,"source":{"id":"https://openalex.org/S76417895","display_name":"Journal of Neuroscience Research","issn_l":"0360-4012","issn":["0360-4012","1097-4547"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Neuroscience Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5006003795","display_name":"Marjorie A. Walz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47838141","display_name":"Saint Louis University","ror":"https://ror.org/01p7jjy08","country_code":"US","type":"education","lineage":["https://openalex.org/I47838141"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"M. A. Walz","raw_affiliation_strings":["Department of Pharmacology, St. Louis University School of Medicine, St. Louis"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, St. Louis University School of Medicine, St. Louis","institution_ids":["https://openalex.org/I47838141"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050605404","display_name":"I. Lee Holt","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47838141","display_name":"Saint Louis University","ror":"https://ror.org/01p7jjy08","country_code":"US","type":"education","lineage":["https://openalex.org/I47838141"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"I. L. Holt","raw_affiliation_strings":["Department of Pharmacology, St. Louis University School of Medicine, St. Louis"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, St. Louis University School of Medicine, St. Louis","institution_ids":["https://openalex.org/I47838141"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015356306","display_name":"A\u00adllyn C. Howlett","orcid":"https://orcid.org/0000-0002-2810-0164","observed_orcids":["https://orcid.org/0000-0002-2810-0164"]},"institutions":[{"id":"https://openalex.org/I47838141","display_name":"Saint Louis University","ror":"https://ror.org/01p7jjy08","country_code":"US","type":"education","lineage":["https://openalex.org/I47838141"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A. C. Howlett","raw_affiliation_strings":["Department of Pharmacology, St. Louis University School of Medicine, St. Louis"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, St. Louis University School of Medicine, St. Louis","institution_ids":["https://openalex.org/I47838141"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5006003795"],"corresponding_institution_ids":["https://openalex.org/I47838141"],"apc_list":{"value":4840,"currency":"USD","value_usd":4840},"apc_paid":null,"fwci":0.2323,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.44562021,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"17","issue":"3","first_page":"291","last_page":"297"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12104","display_name":"Protein Tyrosine Phosphatases","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cyclic-nucleotide-phosphodiesterase","display_name":"cyclic nucleotide phosphodiesterase","score":0.9340000152587891},{"id":"https://openalex.org/keywords/phosphodiesterase-isozymes","display_name":"phosphodiesterase isozymes","score":0.8560000061988831},{"id":"https://openalex.org/keywords/neuroblastoma-cells","display_name":"neuroblastoma cells","score":0.8479999899864197},{"id":"https://openalex.org/keywords/camp-hydrolysis","display_name":"cAMP hydrolysis","score":0.871999979019165},{"id":"https://openalex.org/keywords/cgmp-hydrolysis","display_name":"cGMP hydrolysis","score":1.0},{"id":"https://openalex.org/keywords/thyroid-hormones","display_name":"thyroid hormones","score":0.5899999737739563},{"id":"https://openalex.org/keywords/triiodothyronine","display_name":"triiodothyronine","score":0.9800000190734863},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.675000011920929},{"id":"https://openalex.org/keywords/calmodulin","display_name":"calmodulin","score":0.7699999809265137},{"id":"https://openalex.org/keywords/allosteric-regulation","display_name":"allosteric regulation","score":0.7139999866485596},{"id":"https://openalex.org/keywords/protein-synthesis","display_name":"protein synthesis","score":0.6140000224113464},{"id":"https://openalex.org/keywords/enzyme-regulation","display_name":"enzyme regulation","score":0.4569999873638153}],"concepts":[{"id":"https://openalex.org/C62826618","wikidata":"https://www.wikidata.org/wiki/Q424304","display_name":"Phosphodiesterase","level":3,"score":0.7877388000488281},{"id":"https://openalex.org/C2780215583","wikidata":"https://www.wikidata.org/wiki/Q946700","display_name":"Cyclic nucleotide phosphodiesterase","level":4,"score":0.7088490724563599},{"id":"https://openalex.org/C2778524494","wikidata":"https://www.wikidata.org/wiki/Q967433","display_name":"Cyclic nucleotide","level":4,"score":0.6408872008323669},{"id":"https://openalex.org/C29688787","wikidata":"https://www.wikidata.org/wiki/Q409145","display_name":"Calmodulin","level":3,"score":0.6060367822647095},{"id":"https://openalex.org/C2778128677","wikidata":"https://www.wikidata.org/wiki/Q412895","display_name":"Cycloheximide","level":3,"score":0.5997220873832703},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5082204341888428},{"id":"https://openalex.org/C79418042","wikidata":"https://www.wikidata.org/wiki/Q5864325","display_name":"PDE10A","level":4,"score":0.4438786804676056},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44378119707107544},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.42914363741874695},{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.4273507595062256},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.39055484533309937},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3900882303714752},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.3741346001625061},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3632402718067169},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.17509570717811584},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.05467391014099121},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000001","descriptor_name":"Calcimycin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002147","descriptor_name":"Calmodulin","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D002147","descriptor_name":"Calmodulin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009447","descriptor_name":"Neuroblastoma","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D010726","descriptor_name":"Phosphodiesterase Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014284","descriptor_name":"Triiodothyronine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D015087","descriptor_name":"2',3'-Cyclic-Nucleotide Phosphodiesterases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/jnr.490170314","doi":"https://doi.org/10.1002/jnr.490170314","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jnr.490170314","pdf_url":null,"source":{"id":"https://openalex.org/S76417895","display_name":"Journal of Neuroscience Research","issn_l":"0360-4012","issn":["0360-4012","1097-4547"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Neuroscience Research","raw_type":"journal-article"},{"id":"pmid:3037096","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3037096","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of neuroscience research","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.504800021648407,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3034592056","display_name":"ADENYLATE CYCLASE REGULATION IN NEUROBLASTOMA CELLS","funder_award_id":"R01NS016513","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G8370930516","display_name":"ADENYLATE CYCLASE REGULATION IN NEUROBLASTOMA CELLS","funder_award_id":"K04NS000868","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G8822529380","display_name":"THYROID HORMONE EFFECTS ON CYCLIC NUCLEOTIDE METABOLISM","funder_award_id":"5f32am007205-03","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W139194966","https://openalex.org/W1520716231","https://openalex.org/W1568497312","https://openalex.org/W1581678753","https://openalex.org/W1584222430","https://openalex.org/W1778443220","https://openalex.org/W1981252713","https://openalex.org/W1985630404","https://openalex.org/W1994182953","https://openalex.org/W1997771707","https://openalex.org/W1998225973","https://openalex.org/W2023916700","https://openalex.org/W2024068070","https://openalex.org/W2024316405","https://openalex.org/W2026727806","https://openalex.org/W2030456302","https://openalex.org/W2047404335","https://openalex.org/W2069138715","https://openalex.org/W2123541238","https://openalex.org/W2128635872","https://openalex.org/W2135839082","https://openalex.org/W2139509802","https://openalex.org/W2222808899","https://openalex.org/W2403035831","https://openalex.org/W2415045165","https://openalex.org/W2418901562","https://openalex.org/W2441200514","https://openalex.org/W3025622176","https://openalex.org/W4301381893"],"related_works":["https://openalex.org/W1511344444","https://openalex.org/W2076031094","https://openalex.org/W2068508735","https://openalex.org/W2069138715","https://openalex.org/W2046298739","https://openalex.org/W2076686672","https://openalex.org/W302371127","https://openalex.org/W2123073393","https://openalex.org/W1991233650","https://openalex.org/W2094056941"],"abstract_inverted_index":{"Adenosine":[0],"3',5'-cyclic":[1],"monophosphate":[2],"(cAMP)":[3],"content":[4],"of":[5,14,21,29,59,67,80,88,98,108,119,128,135,137,147,152,167,173,183,186,233,236],"neurons":[6],"is":[7,228],"determined":[8],"not":[9,131],"only":[10],"by":[11,18,23,44,122,210],"the":[12,19,109,138,168,171,214,231],"rate":[13,20],"synthesis":[15],"but":[16,185],"also":[17],"hydrolysis":[22,69,82,107,182],"cyclic":[24,30,111],"nucleotide":[25,31],"phosphodiesterases.":[26],"Multiple":[27],"forms":[28],"phosphodiesterase":[32,121,129,241],"exist":[33],"in":[34,55,133,161,164,170,179,195,200,225,242,245],"brain":[35],"and":[36,39,47,75,94,145],"other":[37,234,243],"tissues,":[38],"these":[40],"may":[41],"be":[42],"regulated":[43],"various":[45],"hormones":[46],"neuromodulators.":[48],"The":[49,221],"present":[50],"study":[51],"examines":[52],"this":[53],"regulation":[54,127],"a":[56,86,96],"cloned":[57],"line":[58],"neuroblastoma":[60,226],"cells":[61,169,227],"(N18TG2).":[62],"A":[63],"biphasic":[64],"Lineweaver-Burk":[65,78],"plot":[66],"cAMP":[68,102,120,184],"revealed":[70],"two":[71],"Kms":[72],"approximating":[73],"5":[74],"25":[76],"microM.":[77,100],"plots":[79],"cGMP":[81,104,123],"were":[83],"linear":[84],"over":[85],"range":[87],"1":[89,92],"microM":[90],"to":[91,192],"mM":[93],"exhibited":[95],"Km":[97],"37":[99],"Neither":[101],"nor":[103],"competed":[105],"for":[106,115],"alternative":[110],"nucleotide.":[112],"No":[113,150],"evidence":[114],"an":[116,180,193],"allosteric":[117],"activation":[118],"was":[124,130,159,190,208],"found.":[125],"Calcium":[126],"found":[132],"spite":[134],"preparation":[136],"cell":[139],"extract":[140],"with":[141,197,230],"several":[142],"protease":[143],"inhibitors,":[144],"addition":[146],"exogenous":[148],"calmodulin.":[149],"effect":[151,217],"calmodulin":[153],"antagonists":[154],"(calmidazolium,":[155],"W7,":[156],"or":[157,163,203],"trifluoperazine)":[158],"observed":[160],"vitro":[162],"situ.":[165],"Growth":[166],"presence":[172],"200":[174],"nM":[175],"3,5,3'-triiodothyronine":[176],"(T3)":[177],"resulted":[178],"increased":[181],"cGMP.":[187],"This":[188,206],"increase":[189,194],"attributed":[191],"Vmax":[196],"no":[198],"change":[199],"either":[201],"high":[202],"low":[204],"Km.":[205],"response":[207,224],"blocked":[209],"cycloheximide,":[211],"suggesting":[212],"that":[213],"thyroid":[215,222,237],"hormone":[216,223,238],"requires":[218],"protein":[219],"synthesis.":[220],"compared":[229],"results":[232],"studies":[235],"effects":[239],"on":[240],"tissues":[244],"vivo.":[246]},"counts_by_year":[],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}