{"id":"https://openalex.org/W2018917476","doi":"https://doi.org/10.1111/j.1432-1033.1985.tb08880.x","title":"Kinetic analysis of lactate dehydrogenase in cultured chondrocytes by quantitative cytochemistry","display_name":"Kinetic analysis of lactate dehydrogenase in cultured chondrocytes by quantitative cytochemistry","publication_year":1985,"publication_date":"1985-05-01","ids":{"openalex":"https://openalex.org/W2018917476","doi":"https://doi.org/10.1111/j.1432-1033.1985.tb08880.x","mag":"2018917476","pmid":"https://pubmed.ncbi.nlm.nih.gov/3996396"},"language":"en","primary_location":{"id":"doi:10.1111/j.1432-1033.1985.tb08880.x","doi":"https://doi.org/10.1111/j.1432-1033.1985.tb08880.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1985.tb08880.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1985.tb08880.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1985.tb08880.x","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5030914696","display_name":"J.-D. Monet","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1288880153","display_name":"H\u00f4pital Necker-Enfants Malades","ror":"https://ror.org/05tr67282","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I1288880153","https://openalex.org/I4210097159","https://openalex.org/I4210120235"]},{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Jean-Dominique MONET","raw_affiliation_strings":["Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]},{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072961806","display_name":"M. Brami","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1288880153","display_name":"H\u00f4pital Necker-Enfants Malades","ror":"https://ror.org/05tr67282","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I1288880153","https://openalex.org/I4210097159","https://openalex.org/I4210120235"]},{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Mona BRAMI","raw_affiliation_strings":["Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]},{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024131691","display_name":"Nicole Dautigny","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1288880153","display_name":"H\u00f4pital Necker-Enfants Malades","ror":"https://ror.org/05tr67282","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I1288880153","https://openalex.org/I4210097159","https://openalex.org/I4210120235"]},{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Nicole DAUTIGNY","raw_affiliation_strings":["Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]},{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081878007","display_name":"J. Assailly","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1288880153","display_name":"H\u00f4pital Necker-Enfants Malades","ror":"https://ror.org/05tr67282","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I1288880153","https://openalex.org/I4210097159","https://openalex.org/I4210120235"]},{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Jacques ASSAILLY","raw_affiliation_strings":["Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]},{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113577106","display_name":"M. Bachelet","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1288880153","display_name":"H\u00f4pital Necker-Enfants Malades","ror":"https://ror.org/05tr67282","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I1288880153","https://openalex.org/I4210097159","https://openalex.org/I4210120235"]},{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Marie BACHELET","raw_affiliation_strings":["Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]},{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Andree ULMANN","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1288880153","display_name":"H\u00f4pital Necker-Enfants Malades","ror":"https://ror.org/05tr67282","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I1288880153","https://openalex.org/I4210097159","https://openalex.org/I4210120235"]},{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Andree ULMANN","raw_affiliation_strings":["Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]},{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111670597","display_name":"Cyrille A. Bader","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1288880153","display_name":"H\u00f4pital Necker-Enfants Malades","ror":"https://ror.org/05tr67282","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I1288880153","https://openalex.org/I4210097159","https://openalex.org/I4210120235"]},{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Cyrille A. BADER","raw_affiliation_strings":["Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (Service Commun N\u00b024 et Unite\u0301 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]},{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Service Commun N\u00b024 et Unit\u00e9 90), Hopital Necker, Paris","institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5111670597"],"corresponding_institution_ids":["https://openalex.org/I1288880153","https://openalex.org/I154526488"],"apc_list":null,"apc_paid":null,"fwci":0.3984,"has_fulltext":true,"cited_by_count":4,"citation_normalized_percentile":{"value":0.60772579,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"148","issue":"3","first_page":"587","last_page":"591"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10105","display_name":"Osteoarthritis Treatment and Mechanisms","score":0.6948000192642212,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10105","display_name":"Osteoarthritis Treatment and Mechanisms","score":0.6948000192642212,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.05000000074505806,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10631","display_name":"Cancer, Hypoxia, and Metabolism","score":0.04479999840259552,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lactate-dehydrogenase","display_name":"lactate dehydrogenase","score":1.0},{"id":"https://openalex.org/keywords/chondrocytes","display_name":"chondrocytes","score":0.9850000143051147},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.9750000238418579},{"id":"https://openalex.org/keywords/quantitative-cytochemistry","display_name":"quantitative cytochemistry","score":0.7369999885559082},{"id":"https://openalex.org/keywords/anaerobic-glycolysis","display_name":"anaerobic glycolysis","score":0.7799999713897705},{"id":"https://openalex.org/keywords/pyruvate-inhibition","display_name":"pyruvate inhibition","score":0.8199999928474426},{"id":"https://openalex.org/keywords/lactate-inhibition","display_name":"lactate inhibition","score":0.6499999761581421},{"id":"https://openalex.org/keywords/nicotinamide-adenine-dinucleotide","display_name":"NAD+","score":0.6949999928474426}],"concepts":[{"id":"https://openalex.org/C2777318727","wikidata":"https://www.wikidata.org/wiki/Q409464","display_name":"Lactate dehydrogenase","level":3,"score":0.8320845365524292},{"id":"https://openalex.org/C2776317432","wikidata":"https://www.wikidata.org/wiki/Q412547","display_name":"Dehydrogenase","level":3,"score":0.6157667636871338},{"id":"https://openalex.org/C2776892249","wikidata":"https://www.wikidata.org/wiki/Q2324668","display_name":"Formazan","level":2,"score":0.5707809329032898},{"id":"https://openalex.org/C75520062","wikidata":"https://www.wikidata.org/wiki/Q15321392","display_name":"NAD+ kinase","level":3,"score":0.551776111125946},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5242484211921692},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.4924103021621704},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.49220505356788635},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4854874908924103},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.4851561188697815},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.43875160813331604},{"id":"https://openalex.org/C2777118752","wikidata":"https://www.wikidata.org/wiki/Q124686","display_name":"Cellulose acetate","level":3,"score":0.43850177526474},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3805278241634369},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3511947989463806},{"id":"https://openalex.org/C2779251873","wikidata":"https://www.wikidata.org/wiki/Q80294","display_name":"Cellulose","level":2,"score":0.16123366355895996},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002356","descriptor_name":"Cartilage","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004586","descriptor_name":"Electrophoresis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D006651","descriptor_name":"Histocytochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007770","descriptor_name":"L-Lactate Dehydrogenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1432-1033.1985.tb08880.x","doi":"https://doi.org/10.1111/j.1432-1033.1985.tb08880.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1985.tb08880.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1985.tb08880.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:3996396","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3996396","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of biochemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1111/j.1432-1033.1985.tb08880.x","doi":"https://doi.org/10.1111/j.1432-1033.1985.tb08880.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1985.tb08880.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1985.tb08880.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2018917476.pdf","grobid_xml":"https://content.openalex.org/works/W2018917476.grobid-xml"},"referenced_works_count":26,"referenced_works":["https://openalex.org/W1485859974","https://openalex.org/W1510290397","https://openalex.org/W1543819219","https://openalex.org/W1546456631","https://openalex.org/W1986511046","https://openalex.org/W1986772821","https://openalex.org/W1995933260","https://openalex.org/W1998728209","https://openalex.org/W2001492617","https://openalex.org/W2001844398","https://openalex.org/W2044093649","https://openalex.org/W2046858377","https://openalex.org/W2049382908","https://openalex.org/W2049967013","https://openalex.org/W2057213037","https://openalex.org/W2078631343","https://openalex.org/W2113478433","https://openalex.org/W2158479853","https://openalex.org/W2164843952","https://openalex.org/W2276445514","https://openalex.org/W2395496929","https://openalex.org/W2396969417","https://openalex.org/W2409945737","https://openalex.org/W2422594871","https://openalex.org/W2756061034","https://openalex.org/W2912336570"],"related_works":["https://openalex.org/W2293234725","https://openalex.org/W2061984624","https://openalex.org/W3170934001","https://openalex.org/W2809439000","https://openalex.org/W2272296835","https://openalex.org/W1987641549","https://openalex.org/W2244803865","https://openalex.org/W4245552537","https://openalex.org/W1967509895","https://openalex.org/W2066623833"],"abstract_inverted_index":{"Kinetic":[0],"analysis":[1,180],"of":[2,48,53,57,74,92,141,155,181],"lactate":[3,110,126,133,182],"dehydrogenase":[4,41,183],"activity":[5,42],"in":[6,12,37,50,145,171,184],"intact":[7],"cultured":[8,22,172,185],"chondrocytes":[9,186],"was":[10,43,63,85,158],"performed":[11,64],"situ":[13],"by":[14,45,65,132,160,197],"coupling":[15],"cell":[16],"culture":[17,194],"and":[18,31,67,90,104,111,114,120,127,138],"microcytophotometry.":[19],"Cells":[20],"were":[21,96],"on":[23,162],"glass":[24],"microscope":[25],"slides":[26],"divided":[27],"into":[28],"eight":[29],"chambers":[30],"studied":[32],"during":[33],"the":[34,46,51,61,71,79,148],"growth":[35],"cycle":[36],"monolayer":[38],"areas.":[39],"Lactate":[40],"assayed":[44],"reduction":[47],"neotetrazolium":[49],"presence":[52],"phenazine":[54],"methosulfate.":[55],"Quantification":[56],"formazan":[58],"deposits":[59],"within":[60],"cells":[62,195],"scanning":[66],"integrating":[68],"microdensitometry":[69],"at":[70],"isosbestic":[72],"wavelength":[73],"585":[75],"nm.":[76],"Results":[77],"indicate":[78],"following":[80],"(a)":[81],"A":[82],"kinetic":[83],"characterization":[84],"possible:":[86],"apparent":[87],"constants,":[88],"Km":[89,115],"Ks":[91,99],"this":[93],"two-substrate":[94],"enzyme":[95],"graphically":[97],"determined":[98],"=":[100,116],"1.05":[101],"+/-":[102,106,118,122],"0.08":[103],"0.56":[105],"0.05":[107],"mM":[108,124,137],"for":[109,125],"NAD":[112,128],"respectively":[113],"0.64":[117],"0.03":[119],"0.37":[121],"0.02":[123],"respectively.":[129],"(b)":[130],"Inhibition":[131],"concentrations":[134],"above":[135],"10":[136],"pyruvate":[139],"concentration":[140],"1":[142],"mM,":[143],"is":[144],"agreement":[146],"with":[147],"well":[149],"known":[150],"high":[151],"anaerobic":[152],"glycolytic":[153],"metabolism":[154],"chondrocytes.":[156,174],"This":[157,175],"confirmed":[159],"electrophoresis":[161],"cellulose":[163],"acetate":[164],"which":[165],"demonstrated":[166],"a":[167],"M3-H":[168],"isoenzyme":[169],"form":[170],"chick":[173],"study":[176],"shows":[177],"that":[178],"microcytophotometric":[179],"may":[187],"be":[188],"an":[189],"interesting":[190],"alternative":[191],"to":[192],"mass":[193],"followed":[196],"classical":[198],"biochemical":[199],"studies.":[200]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}