{"id":"https://openalex.org/W2018917443","doi":"https://doi.org/10.1017/s0967199401001083","title":"A combination of calcium ionophore and puromycin effectively produces human parthenogenones with one haploid pronucleus","display_name":"A combination of calcium ionophore and puromycin effectively produces human parthenogenones with one haploid pronucleus","publication_year":2001,"publication_date":"2001-02-01","ids":{"openalex":"https://openalex.org/W2018917443","doi":"https://doi.org/10.1017/s0967199401001083","mag":"2018917443","pmid":"https://pubmed.ncbi.nlm.nih.gov/11273035"},"language":"en","primary_location":{"id":"doi:10.1017/s0967199401001083","doi":"https://doi.org/10.1017/s0967199401001083","is_oa":false,"landing_page_url":"https://doi.org/10.1017/s0967199401001083","pdf_url":null,"source":{"id":"https://openalex.org/S99576920","display_name":"Zygote","issn_l":"0967-1994","issn":["0967-1994","1469-8730"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311721","host_organization_name":"Cambridge University Press","host_organization_lineage":["https://openalex.org/P4310311721","https://openalex.org/P4310311702"],"host_organization_lineage_names":["Cambridge University Press","University of Cambridge"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zygote","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009711341","display_name":"Koji Nakagawa","orcid":"https://orcid.org/0000-0003-0874-5894","observed_orcids":["https://orcid.org/0000-0003-0874-5894"]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Koji Nakagawa","raw_affiliation_strings":["Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Japan","Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Tokushima, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108383396","display_name":"Shuji Yamano","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shuji Yamano","raw_affiliation_strings":["Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Tokushima, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059517204","display_name":"Hisayo Nakasaka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hisayo Nakasaka","raw_affiliation_strings":["Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Tokushima, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016695302","display_name":"Kenji Hinokio","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kenji Hinokio","raw_affiliation_strings":["Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Tokushima, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108571882","display_name":"Midori Yoshizawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I207399273","display_name":"Utsunomiya University","ror":"https://ror.org/05bx1gz93","country_code":"JP","type":"education","lineage":["https://openalex.org/I207399273"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Midori Yoshizawa","raw_affiliation_strings":["Department of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University, Utsunomiya, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University, Utsunomiya, Japan","institution_ids":["https://openalex.org/I207399273"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111735956","display_name":"Toshihiro Aono","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Toshihiro Aono","raw_affiliation_strings":["Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Tokushima, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Obstetrics and Gynaecology, School of Medicine, The University of Tokushima, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3550,"currency":"USD","value_usd":3550},"apc_paid":null,"fwci":5.8303,"has_fulltext":false,"cited_by_count":57,"citation_normalized_percentile":{"value":0.96642781,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"9","issue":"1","first_page":"83","last_page":"88"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.004900000058114529,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/parthenogenetic-activation","display_name":"parthenogenetic activation","score":0.9340000152587891},{"id":"https://openalex.org/keywords/a23187","display_name":"A23187","score":0.9259999990463257},{"id":"https://openalex.org/keywords/puromycin","display_name":"puromycin","score":0.9990000128746033},{"id":"https://openalex.org/keywords/6-dimethylaminopurine","display_name":"6-dimethylaminopurine","score":0.9679999947547913},{"id":"https://openalex.org/keywords/second-polar-body","display_name":"second polar body","score":0.6949999928474426},{"id":"https://openalex.org/keywords/haploid-chromosomes","display_name":"haploid chromosomes","score":0.5770000219345093},{"id":"https://openalex.org/keywords/protein-synthesis-inhibition","display_name":"protein synthesis inhibition","score":0.6710000038146973},{"id":"https://openalex.org/keywords/oocyte-activation","display_name":"oocyte activation","score":0.4740000069141388}],"concepts":[{"id":"https://openalex.org/C2779200571","wikidata":"https://www.wikidata.org/wiki/Q424696","display_name":"Puromycin","level":3,"score":0.9473104476928711},{"id":"https://openalex.org/C110758877","wikidata":"https://www.wikidata.org/wiki/Q2614103","display_name":"Pronucleus","level":5,"score":0.9458625316619873},{"id":"https://openalex.org/C2779014961","wikidata":"https://www.wikidata.org/wiki/Q415168","display_name":"Ionophore","level":3,"score":0.6805163025856018},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.6193689703941345},{"id":"https://openalex.org/C519063684","wikidata":"https://www.wikidata.org/wiki/Q706","display_name":"Calcium","level":2,"score":0.5746563076972961},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5633200407028198},{"id":"https://openalex.org/C2909946033","wikidata":"https://www.wikidata.org/wiki/Q2614103","display_name":"Male pronucleus","level":5,"score":0.5542200207710266},{"id":"https://openalex.org/C179265757","wikidata":"https://www.wikidata.org/wiki/Q7095247","display_name":"Oocyte activation","level":4,"score":0.5257447957992554},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.4136379063129425},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.334697961807251},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.25468164682388306},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24895942211151123},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22330299019813538},{"id":"https://openalex.org/C28748434","wikidata":"https://www.wikidata.org/wiki/Q170145","display_name":"Zygote","level":4,"score":0.1701555848121643},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.12606742978096008},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10924702882766724},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C43369102","wikidata":"https://www.wikidata.org/wiki/Q2307625","display_name":"Fracture (geology)","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000001","descriptor_name":"Calcimycin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D000225","descriptor_name":"Adenine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D000225","descriptor_name":"Adenine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001755","descriptor_name":"Blastocyst","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002877","descriptor_name":"Chromosomes, Human","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004791","descriptor_name":"Enzyme Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005307","descriptor_name":"Fertilization in Vitro","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006238","descriptor_name":"Haploidy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007476","descriptor_name":"Ionophores","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D009865","descriptor_name":"Oocytes","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D009865","descriptor_name":"Oocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D010312","descriptor_name":"Parthenogenesis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D011500","descriptor_name":"Protein Synthesis Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011691","descriptor_name":"Puromycin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1017/s0967199401001083","doi":"https://doi.org/10.1017/s0967199401001083","is_oa":false,"landing_page_url":"https://doi.org/10.1017/s0967199401001083","pdf_url":null,"source":{"id":"https://openalex.org/S99576920","display_name":"Zygote","issn_l":"0967-1994","issn":["0967-1994","1469-8730"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311721","host_organization_name":"Cambridge University Press","host_organization_lineage":["https://openalex.org/P4310311721","https://openalex.org/P4310311702"],"host_organization_lineage_names":["Cambridge University Press","University of Cambridge"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zygote","raw_type":"journal-article"},{"id":"pmid:11273035","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11273035","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zygote (Cambridge, England)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.504800021648407,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W987526729","https://openalex.org/W1434994921","https://openalex.org/W1973206828","https://openalex.org/W2012130857","https://openalex.org/W2022830021","https://openalex.org/W2023149156","https://openalex.org/W2023640845","https://openalex.org/W2042447008","https://openalex.org/W2067486558","https://openalex.org/W2086260749","https://openalex.org/W2139319210","https://openalex.org/W2266109448"],"related_works":["https://openalex.org/W2027757084","https://openalex.org/W2351370501","https://openalex.org/W2086260749","https://openalex.org/W1502340163","https://openalex.org/W2258789037","https://openalex.org/W2066693111","https://openalex.org/W2083444435","https://openalex.org/W7099236174","https://openalex.org/W2266109448","https://openalex.org/W1967784577"],"abstract_inverted_index":{"Parthenogenetic":[0],"activation":[1,89],"with":[2,25,43,65,146,194,222],"various":[3],"combinations":[4],"of":[5,21,88,139,155,205,212],"the":[6,77,108,118,134,140,147,156,160,164,170,178,195,198,210],"calcium":[7],"ionophore":[8],"A23187":[9,37,66,102,213],"and":[10,92,98,110,123,214],"protein":[11],"synthesis":[12],"or":[13,51],"phosphorylation":[14],"inhibitors":[15],"was":[16,94,173],"investigated":[17],"as":[18,68],"a":[19,69,202],"means":[20],"producing":[22,219],"human":[23,30,220],"parthenogenones":[24,221],"one":[26,144,192,223],"haploid":[27,203,224],"pronucleus.":[28,225],"Unfertilised":[29],"aged":[31],"oocytes":[32,142,158,189],"exposed":[33],"to":[34,84],"5":[35,39,60],"microM":[36],"for":[38,59,82,218],"min":[40],"were":[41,80,113],"treated":[42,63],"10":[44],"microg/ml":[45],"puromycin":[46,109,135,171,199,215],"(puromycin":[47],"group,":[48,56,72,136],"46":[49],"oocytes)":[50,58],"2":[52],"mM":[53],"6-dimethylaminopurine":[54],"(DMAP":[55],"42":[57,99],"h.":[61,86],"Oocytes":[62],"only":[64],"served":[67],"control":[70,119],"(control":[71],"40":[73],"oocytes).":[74],"After":[75],"washing":[76],"oocytes,":[78],"they":[79],"incubated":[81],"up":[83],"37":[85],"Evidence":[87],"(pronuclear":[90],"formation)":[91],"cleavage":[93,167],"observed":[95],"18":[96],"h":[97,100],"after":[101],"treatment,":[103],"respectively.":[104],"Activation":[105],"rates":[106],"in":[107,117,159,169,177,197],"DMAP":[111,161,179],"groups":[112],"significantly":[114,174],"higher":[115],"than":[116,176],"group":[120,162,172,180,200],"(91%":[121],"(42/46)":[122],"77%":[124],"(34/44)":[125],"vs":[126,182],"20%":[127],"(8/40),":[128],"p":[129,184],"<":[130,185],"0.05,":[131],"respectively).":[132],"In":[133,208],"81%":[137],"(34/42)":[138],"activated":[141,157,188],"showed":[143,201],"pronucleus":[145,193],"second":[148],"polar":[149],"body":[150],"(2ndPB),":[151],"whereas":[152],"none":[153],"(0/34)":[154],"extruded":[163],"2ndPB.":[165],"The":[166,187],"rate":[168],"lower":[175],"(38%":[181],"68%,":[183],"0.05).":[186],"which":[190],"had":[191],"2ndPB":[196],"set":[204],"chromosomes":[206],"(10/13).":[207],"conclusion,":[209],"combination":[211],"is":[216],"effective":[217]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
