{"id":"https://openalex.org/W2018804778","doi":"https://doi.org/10.1046/j.1365-2958.1998.00679.x","title":"Type II protein secretion by Pseudomonas aeruginosa: genetic suppression of a conditional mutation in the pilin\u2010like component XcpT by the cytoplasmic component XcpR","display_name":"Type II protein secretion by Pseudomonas aeruginosa: genetic suppression of a conditional mutation in the pilin\u2010like component XcpT by the cytoplasmic component XcpR","publication_year":1998,"publication_date":"1998-01-01","ids":{"openalex":"https://openalex.org/W2018804778","doi":"https://doi.org/10.1046/j.1365-2958.1998.00679.x","mag":"2018804778","pmid":"https://pubmed.ncbi.nlm.nih.gov/9466269"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-2958.1998.00679.x","doi":"https://doi.org/10.1046/j.1365-2958.1998.00679.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2958.1998.00679.x","pdf_url":null,"source":{"id":"https://openalex.org/S102549058","display_name":"Molecular Microbiology","issn_l":"0950-382X","issn":["0950-382X","1365-2958"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5067407189","display_name":"Yoshihiro Kagami","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]},{"id":"https://openalex.org/I2801919071","display_name":"University of Illinois System","ror":"https://ror.org/05e94g991","country_code":"US","type":"education","lineage":["https://openalex.org/I2801919071"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yoshihiro Kagami","raw_affiliation_strings":["Department of Microbiology, University of Illinois, Urbana 61801, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of Illinois, Urbana 61801, USA","institution_ids":["https://openalex.org/I157725225","https://openalex.org/I2801919071"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010853278","display_name":"Melanie Ratliff","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Melanie Ratliff","raw_affiliation_strings":["B103 Chemical and Life Sciences Laboratories, Department of Microbiology MC-110, 601 South Goodwin Ave., University of Illinois, Urbana, IL 61801, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"B103 Chemical and Life Sciences Laboratories, Department of Microbiology MC-110, 601 South Goodwin Ave., University of Illinois, Urbana, IL 61801, USA","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079745319","display_name":"Mark W. Surber","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark Surber","raw_affiliation_strings":["B103 Chemical and Life Sciences Laboratories, Department of Microbiology MC-110, 601 South Goodwin Ave., University of Illinois, Urbana, IL 61801, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"B103 Chemical and Life Sciences Laboratories, Department of Microbiology MC-110, 601 South Goodwin Ave., University of Illinois, Urbana, IL 61801, USA","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Alejandro Martinez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alejandro Martinez","raw_affiliation_strings":["B103 Chemical and Life Sciences Laboratories, Department of Microbiology MC-110, 601 South Goodwin Ave., University of Illinois, Urbana, IL 61801, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"B103 Chemical and Life Sciences Laboratories, Department of Microbiology MC-110, 601 South Goodwin Ave., University of Illinois, Urbana, IL 61801, USA","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"last","author":{"id":null,"display_name":"David N. Nunn","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"David N. Nunn","raw_affiliation_strings":["B103 Chemical and Life Sciences Laboratories, Department of Microbiology MC-110, 601 South Goodwin Ave., University of Illinois, Urbana, IL 61801, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"B103 Chemical and Life Sciences Laboratories, Department of Microbiology MC-110, 601 South Goodwin Ave., University of Illinois, Urbana, IL 61801, USA","institution_ids":["https://openalex.org/I157725225"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I157725225"],"apc_list":{"value":4430,"currency":"USD","value_usd":4430},"apc_paid":null,"fwci":4.6957,"has_fulltext":false,"cited_by_count":72,"citation_normalized_percentile":{"value":0.95666785,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"27","issue":"1","first_page":"221","last_page":"233"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9750999808311462,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9750999808311462,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.012500000186264515,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10593","display_name":"Bacterial biofilms and quorum sensing","score":0.0032999999821186066,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/type-ii-secretion-system","display_name":"type II secretion system","score":0.8659999966621399},{"id":"https://openalex.org/keywords/pseudomonas-aeruginosa","display_name":"Pseudomonas aeruginosa","score":0.9559999704360962},{"id":"https://openalex.org/keywords/general-secretion-pathway","display_name":"general secretion pathway","score":0.621999979019165},{"id":"https://openalex.org/keywords/temperature-sensitive-mutation","display_name":"temperature-sensitive mutation","score":0.656000018119812},{"id":"https://openalex.org/keywords/extragenic-suppressors","display_name":"extragenic suppressors","score":0.8899999856948853},{"id":"https://openalex.org/keywords/conditional-mutations","display_name":"conditional mutations","score":0.5450000166893005},{"id":"https://openalex.org/keywords/type-iv-pili","display_name":"type IV pili","score":0.7319999933242798},{"id":"https://openalex.org/keywords/protein-secretion","display_name":"protein secretion","score":0.5979999899864197},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.8809999823570251}],"concepts":[{"id":"https://openalex.org/C2776803919","wikidata":"https://www.wikidata.org/wiki/Q7194029","display_name":"Pilin","level":5,"score":0.9625409841537476},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8741868734359741},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6924165487289429},{"id":"https://openalex.org/C174832134","wikidata":"https://www.wikidata.org/wiki/Q934786","display_name":"Pilus","level":4,"score":0.6878630518913269},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.6812715530395508},{"id":"https://openalex.org/C195286587","wikidata":"https://www.wikidata.org/wiki/Q424689","display_name":"Gene product","level":4,"score":0.6234641671180725},{"id":"https://openalex.org/C131934819","wikidata":"https://www.wikidata.org/wiki/Q135390","display_name":"Biogenesis","level":3,"score":0.5987949967384338},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5756180286407471},{"id":"https://openalex.org/C2777637488","wikidata":"https://www.wikidata.org/wiki/Q31856","display_name":"Pseudomonas aeruginosa","level":3,"score":0.5418558716773987},{"id":"https://openalex.org/C36857842","wikidata":"https://www.wikidata.org/wiki/Q1501261","display_name":"Gene cluster","level":3,"score":0.5297273993492126},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.46584782004356384},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.41940993070602417},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.3012140989303589},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.234730064868927},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2104170322418213},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.20221292972564697}],"mesh":[{"descriptor_ui":"D001425","descriptor_name":"Bacterial Outer Membrane Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011550","descriptor_name":"Pseudomonas aeruginosa","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011550","descriptor_name":"Pseudomonas aeruginosa","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D013489","descriptor_name":"Suppression, Genetic","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D026901","descriptor_name":"Membrane Transport Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1046/j.1365-2958.1998.00679.x","doi":"https://doi.org/10.1046/j.1365-2958.1998.00679.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2958.1998.00679.x","pdf_url":null,"source":{"id":"https://openalex.org/S102549058","display_name":"Molecular Microbiology","issn_l":"0950-382X","issn":["0950-382X","1365-2958"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Microbiology","raw_type":"journal-article"},{"id":"pmid:9466269","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9466269","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular microbiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G8751636900","display_name":null,"funder_award_id":"AI32143","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G8852772572","display_name":"Cellular and Molecular Biology Training Grant","funder_award_id":"T32GM007283","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W1489955058","https://openalex.org/W1510702873","https://openalex.org/W1518675858","https://openalex.org/W1524907729","https://openalex.org/W1546783991","https://openalex.org/W1569560298","https://openalex.org/W1707289889","https://openalex.org/W1849997836","https://openalex.org/W1914922901","https://openalex.org/W1932645010","https://openalex.org/W1968861577","https://openalex.org/W1975085160","https://openalex.org/W1994921384","https://openalex.org/W1997479358","https://openalex.org/W2004277005","https://openalex.org/W2005206340","https://openalex.org/W2019500447","https://openalex.org/W2026174297","https://openalex.org/W2053211842","https://openalex.org/W2066537459","https://openalex.org/W2120339843","https://openalex.org/W2142927603","https://openalex.org/W2144113817","https://openalex.org/W2149840779","https://openalex.org/W2152737402","https://openalex.org/W2159122632","https://openalex.org/W2159799356","https://openalex.org/W2161302487","https://openalex.org/W2888511473","https://openalex.org/W2915782266"],"related_works":["https://openalex.org/W2591086900","https://openalex.org/W2004808352","https://openalex.org/W2155247823","https://openalex.org/W1973770205","https://openalex.org/W2588375853","https://openalex.org/W2148948559","https://openalex.org/W2105083008","https://openalex.org/W2024006534","https://openalex.org/W2124779918","https://openalex.org/W1934623513"],"abstract_inverted_index":{"Pseudomonas":[0],"aeruginosa":[1,116],"exports":[2],"a":[3,20,107,162,173],"number":[4,42],"of":[5,22,29,43,49,51,100,113,132,145,189],"hydrolytic":[6],"enzymes":[7],"and":[8,25,36,74,87,127,150,161,171,182],"toxins":[9],"using":[10],"the":[11,27,52,67,82,90,101,111,124,133,146,167,185,190],"type":[12,53],"II":[13],"or":[14,187],"general":[15],"secretion":[16],"pathway,":[17],"found":[18],"in":[19,38,70,79,123],"variety":[21],"Gram-negative":[23],"bacteria":[24],"requiring":[26],"functions":[28,155],"at":[30],"least":[31],"12":[32],"gene":[33,45,126,148,169],"products":[34,46],"(XcpP-Z":[35],"PilD/XcpA":[37,91],"P.":[39,115],"aeruginosa).":[40],"A":[41],"these":[44],"are":[47,63,85,104,157],"homologues":[48],"components":[50,99],"IV":[54],"pilus":[55],"biogenesis":[56],"system,":[57],"including":[58],"four":[59],"proteins,":[60,78],"XcpT-W,":[61],"which":[62],"highly":[64],"similar":[65],"to":[66,81,166],"pilin":[68,83],"subunit":[69],"their":[71,95],"size,":[72],"localization":[73],"post-translational":[75],"modifications.":[76],"These":[77,135],"addition":[80],"subunit,":[84],"cleaved":[86],"methylated":[88],"by":[89],"prepilin":[92],"peptidase,":[93],"but":[94],"interactions":[96],"with":[97],"other":[98],"export":[102,191],"apparatus":[103],"unclear.":[105],"Using":[106],"medium":[108],"developed":[109],"for":[110,130,159,184],"selection":[112],"export-proficient":[114],"strains,":[117],"we":[118],"have":[119],"isolated":[120],"temperature-sensitive":[121],"mutations":[122],"xcpT":[125],"extragenic":[128],"suppressors":[129,136],"one":[131,141],"mutants.":[134],"fall":[137],"into":[138],"two":[139,178],"classes,":[140],"that":[142,156,164],"maps":[143,165],"outside":[144],"xcpP-Z":[147],"cluster":[149],"may":[151],"define":[152],"additional":[153],"cellular":[154,180],"required":[158,183],"export,":[160],"second":[163],"xcpR":[168],"product":[170],"indicates":[172],"potential":[174],"protein-protein":[175],"interaction":[176],"connecting":[177],"different":[179],"compartments":[181],"assembly":[186],"function":[188],"apparatus.":[192]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}