{"id":"https://openalex.org/W2018789442","doi":"https://doi.org/10.1158/1940-6207.prev-08-a69","title":"Abstract A69: mRNA and microRNA profiling of the microenvironment in prostate biopsies: Valuable tool for risk assessment and prevention trials","display_name":"Abstract A69: mRNA and microRNA profiling of the microenvironment in prostate biopsies: Valuable tool for risk assessment and prevention trials","publication_year":2008,"publication_date":"2008-11-01","ids":{"openalex":"https://openalex.org/W2018789442","doi":"https://doi.org/10.1158/1940-6207.prev-08-a69","mag":"2018789442"},"language":"en","primary_location":{"id":"doi:10.1158/1940-6207.prev-08-a69","doi":"https://doi.org/10.1158/1940-6207.prev-08-a69","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1940-6207.prev-08-a69","pdf_url":null,"source":{"id":"https://openalex.org/S68240649","display_name":"Cancer Prevention Research","issn_l":"1940-6207","issn":["1940-6207","1940-6215"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Prevention Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5019107791","display_name":"Larisa Nonn","orcid":"https://orcid.org/0000-0002-0852-7726","observed_orcids":["https://orcid.org/0000-0002-0852-7726"]},"institutions":[{"id":"https://openalex.org/I39422238","display_name":"University of Illinois Chicago","ror":"https://ror.org/02mpq6x41","country_code":"US","type":"education","lineage":["https://openalex.org/I39422238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Larisa Nonn","raw_affiliation_strings":["University of Illinois at Chicago, Chicago, IL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Illinois at Chicago, Chicago, IL","institution_ids":["https://openalex.org/I39422238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018972614","display_name":"Avani Vaishnav","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I39422238","display_name":"University of Illinois Chicago","ror":"https://ror.org/02mpq6x41","country_code":"US","type":"education","lineage":["https://openalex.org/I39422238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Avani Vaishnav","raw_affiliation_strings":["University of Illinois at Chicago, Chicago, IL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Illinois at Chicago, Chicago, IL","institution_ids":["https://openalex.org/I39422238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031283803","display_name":"Vijayalakshmi Ananthanarayanan","orcid":"https://orcid.org/0009-0001-3993-4404","observed_orcids":["https://orcid.org/0009-0001-3993-4404"]},"institutions":[{"id":"https://openalex.org/I39422238","display_name":"University of Illinois Chicago","ror":"https://ror.org/02mpq6x41","country_code":"US","type":"education","lineage":["https://openalex.org/I39422238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vijayalakshmi Ananthanarayanan","raw_affiliation_strings":["University of Illinois at Chicago, Chicago, IL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Illinois at Chicago, Chicago, IL","institution_ids":["https://openalex.org/I39422238"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Lindsay Gallagher","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I39422238","display_name":"University of Illinois Chicago","ror":"https://ror.org/02mpq6x41","country_code":"US","type":"education","lineage":["https://openalex.org/I39422238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lindsay Gallagher","raw_affiliation_strings":["University of Illinois at Chicago, Chicago, IL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Illinois at Chicago, Chicago, IL","institution_ids":["https://openalex.org/I39422238"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5022722673","display_name":"Peter H. Gann","orcid":"https://orcid.org/0000-0002-9439-2900","observed_orcids":["https://orcid.org/0000-0002-9439-2900"]},"institutions":[{"id":"https://openalex.org/I39422238","display_name":"University of Illinois Chicago","ror":"https://ror.org/02mpq6x41","country_code":"US","type":"education","lineage":["https://openalex.org/I39422238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peter Gann","raw_affiliation_strings":["University of Illinois at Chicago, Chicago, IL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Illinois at Chicago, Chicago, IL","institution_ids":["https://openalex.org/I39422238"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I39422238"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"1","issue":"7_Supplement","first_page":"A69","last_page":"A69"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.7143999934196472,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.7143999934196472,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10543","display_name":"Prostate Cancer Treatment and Research","score":0.11330000311136246,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10124","display_name":"Prostate Cancer Diagnosis and Treatment","score":0.08309999853372574,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/prostate-cancer","display_name":"prostate cancer","score":0.996999979019165},{"id":"https://openalex.org/keywords/microrna-expression-profiling","display_name":"miRNA expression profiling","score":0.7039999961853027},{"id":"https://openalex.org/keywords/mrna-profiling","display_name":"mRNA expression profiling","score":0.875},{"id":"https://openalex.org/keywords/formalin-fixed-paraffin-embedded-tissue","display_name":"formalin-fixed paraffin-embedded tissue","score":0.9520000219345093},{"id":"https://openalex.org/keywords/laser-capture-microdissection","display_name":"laser capture microdissection","score":0.9179999828338623},{"id":"https://openalex.org/keywords/tumor-microenvironment","display_name":"tumor microenvironment","score":0.6899999976158142},{"id":"https://openalex.org/keywords/stromal-compartment","display_name":"stromal compartment","score":0.7110000252723694},{"id":"https://openalex.org/keywords/pca3","display_name":"PCA3","score":0.5389999747276306},{"id":"https://openalex.org/keywords/alpha-methylacyl-coa-racemase","display_name":"AMACR","score":0.9179999828338623},{"id":"https://openalex.org/keywords/nkx3-1","display_name":"NKX3.1","score":0.9990000128746033},{"id":"https://openalex.org/keywords/mir-125b","display_name":"miR-125b","score":0.972000002861023},{"id":"https://openalex.org/keywords/mir-22","display_name":"miR-22","score":0.9959999918937683},{"id":"https://openalex.org/keywords/mir-16","display_name":"miR-16","score":0.9990000128746033},{"id":"https://openalex.org/keywords/risk-assessment","display_name":"risk assessment","score":0.46399998664855957},{"id":"https://openalex.org/keywords/biomarkers","display_name":"biomarkers","score":0.5799999833106995},{"id":"https://openalex.org/keywords/prostate-biopsy","display_name":"prostate biopsy","score":0.4569999873638153}],"concepts":[{"id":"https://openalex.org/C2780192828","wikidata":"https://www.wikidata.org/wiki/Q181257","display_name":"Prostate cancer","level":3,"score":0.7317954301834106},{"id":"https://openalex.org/C2776235491","wikidata":"https://www.wikidata.org/wiki/Q9625","display_name":"Prostate","level":3,"score":0.71806800365448},{"id":"https://openalex.org/C125965507","wikidata":"https://www.wikidata.org/wiki/Q1516595","display_name":"Laser capture microdissection","level":4,"score":0.6837829351425171},{"id":"https://openalex.org/C16930146","wikidata":"https://www.wikidata.org/wiki/Q4381253","display_name":"Stromal cell","level":2,"score":0.6371692419052124},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.5745357871055603},{"id":"https://openalex.org/C87874733","wikidata":"https://www.wikidata.org/wiki/Q18039844","display_name":"PCA3","level":4,"score":0.5496070981025696},{"id":"https://openalex.org/C2781217009","wikidata":"https://www.wikidata.org/wiki/Q4086927","display_name":"Prostate biopsy","level":4,"score":0.5249164700508118},{"id":"https://openalex.org/C2775934546","wikidata":"https://www.wikidata.org/wiki/Q179991","display_name":"Biopsy","level":2,"score":0.5196251273155212},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.4806000888347626},{"id":"https://openalex.org/C11242284","wikidata":"https://www.wikidata.org/wiki/Q4293202","display_name":"Microdissection","level":3,"score":0.47408902645111084},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4037303328514099},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.3898942470550537},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3830263018608093},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.36197376251220703},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.3434479832649231},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.18660825490951538},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.15674856305122375},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1940-6207.prev-08-a69","doi":"https://doi.org/10.1158/1940-6207.prev-08-a69","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1940-6207.prev-08-a69","pdf_url":null,"source":{"id":"https://openalex.org/S68240649","display_name":"Cancer Prevention Research","issn_l":"1940-6207","issn":["1940-6207","1940-6215"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Prevention Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9537000060081482,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1974449618","https://openalex.org/W1461969930","https://openalex.org/W2028652213","https://openalex.org/W2095479337","https://openalex.org/W4391430176","https://openalex.org/W2021639559","https://openalex.org/W7095465967","https://openalex.org/W2891725744","https://openalex.org/W2009349958","https://openalex.org/W3021034518"],"abstract_inverted_index":{"Abstract":[0],"A69":[1],"Due":[2],"to":[3,71,94,107,120,139,277,285,369,388,441,447,451,458],"the":[4,24,36,96,105,118,191,199,227,230,259,301,321,334,361,366,380,385,419,427,432,436],"widespread":[5],"use":[6],"of":[7,11,23,28,98,113,201,211,247,281,312,346,406,422,431,463,471],"PSA":[8],"testing,":[9],"diagnosis":[10],"PCa":[12,239,275,289,308,329,350,362],"typically":[13],"relies":[14],"on":[15],"needle":[16],"biopsies,":[17,102],"which":[18,40,103,481],"provide":[19],"sparse,":[20],"random":[21],"sampling":[22],"prostate.":[25],"About":[26],"70-80%":[27],"these":[29,85,295,326,347],"biopsies":[30,170],"are":[31,46,128,142],"negative":[32],"for":[33,132,243,325],"cancer,":[34],"and":[35,76,90,152,162,198,203,229,238,253,261,267,271,307,320,328,376,383,408,435,483],"non-malignant":[37],"tissue":[38,147,189,232,240,335,359,394],"samples,":[39,423],"may":[41,154,351],"contain":[42],"valuable":[43],"risk":[44],"information,":[45],"routinely":[47],"archived":[48],"in":[49,58,145,158,182,233,274,288,300,349,360,392,418,426,456,474,480],"paraffin":[50],"after":[51],"formalin":[52],"fixation.":[53],"Although":[54],"RNA":[55,195,372,404,424,460,472],"is":[56,330,365,413,446],"degraded":[57],"formalin-fixed":[59],"paraffin-embedded":[60],"(FFPE)":[61],"tissue,":[62],"recent":[63],"studies":[64],"have":[65,88,297],"shown":[66],"that,":[67],"with":[68,100,109,401],"adequate":[69],"attention":[70],"methodological":[72],"detail,":[73],"meaningful":[74],"gene":[75],"microRNA":[77],"(miRNA)":[78],"expression":[79,200,299,345,373,391,425,438],"data":[80],"can":[81],"be":[82,155,286,352],"obtained":[83],"from":[84,167,176,186,190,356,374,379,395,410],"specimens.":[86],"We":[87,398],"optimized":[89],"validated":[91],"several":[92],"methods":[93,466],"overcome":[95],"challenges":[97],"working":[99],"prostate":[101,136,159,169,396,434,453,475],"requires":[104],"ability":[106,119],"work":[108],"extremely":[110],"limited":[111],"amounts":[112],"material":[114,412,485],"as":[115,117],"well":[116],"isolate":[121],"relatively":[122],"homogeneous":[123],"cell":[124],"populations.":[125],"miRNA":[126,313,390,409],"signatures":[127],"rapidly":[129],"being":[130],"identified":[131],"many":[133],"cancers":[134],"including":[135],"cancer.":[137],"Compared":[138],"mRNAs,":[140],"miRNAs":[141,204,283,296,327,348],"highly":[143],"stable":[144],"FFPE":[146,168,375],"due":[148],"their":[149],"small":[150,420],"size,":[151],"thus":[153],"ideal":[156],"biomarkers":[157],"biopsies.":[160,397],"Epithelial":[161],"stromal":[163,302,358],"tissues":[164,378],"were":[165,269],"collected":[166,333],"by":[171,206,336],"laser":[172],"capture":[173],"microdissection":[174],"(LCM)":[175],"three":[177,235,248,282],"patients.":[178,193,236,245],"Tissue":[179],"was":[180,196,241,429,439],"LCM-collected":[181,393],"a":[183],"similar":[184,310,440],"manner":[185],"paired":[187],"frozen":[188,231,262,377,442],"same":[192,381],"Total":[194],"isolated":[197],"mRNA":[202,407],"analyzed":[205],"qRT-PCR":[207],"(mention":[208],"PreAmp).":[209],"Expression":[210,246],"7":[212],"epithelial":[213],"or":[214],"stromal-specific":[215],"genes":[216,250],"(K18,":[217],"PSA,":[218],"NKX3.1,":[219],"AMACR,":[220],"IGF1,":[221],"TIMP3,":[222],"Desmin)":[223],"showed":[224,256,293,309],"agreement":[225,257],"between":[226,258,317],"FFPE-biopsies":[228,260],"all":[234],"Normal":[237],"available":[242],"two":[244],"PCa-related":[249],"(PCA3,":[251],"AMACR":[252,268],"NKX3.1)":[254],"also":[255,384,468],"tissue.":[263],"As":[264],"predicted,":[265],"PCA3":[266],"elevated":[270],"NKX3.1":[272],"decreased":[273,344,357],"relative":[276,437],"normal":[278,305],"areas.":[279],"Analysis":[280],"reported":[284],"down-regulated":[287],"(miR-125b,":[290],"miR-22,":[291],"miR-16)":[292],"that":[294,331,340,400],"higher":[298],"compartment.":[303],"However,":[304],"epithelium":[306],"levels":[311],"expression.":[314],"The":[315],"difference":[316],"our":[318],"methodology":[319,450],"previously":[322],"published":[323],"reports":[324],"we":[332],"LCM.":[337],"This":[338,364],"suggests":[339],"previous":[341],"findings":[342],"showing":[343],"an":[353],"artifact":[354],"resulting":[355],"lesions.":[363],"first":[367,386],"study":[368,387],"directly":[370],"compare":[371],"patient":[382],"examine":[389],"show":[399],"this":[402,449],"methodology,":[403],"profiling":[405],"FFPE-biopsy":[411,428],"quite":[414],"feasible.":[415],"More":[416],"importantly,":[417],"set":[421],"reflective":[430],"whole":[433],"material.":[443],"Our":[444],"goal":[445],"apply":[448],"archival":[452],"biopsy":[454],"banks":[455],"order":[457],"identify":[459],"markers":[461,473],"predictive":[462],"PCa.":[464],"These":[465],"will":[467],"facilitate":[469],"measurement":[470],"cancer":[476],"prevention":[477],"clinical":[478],"trials":[479],"pre":[482],"post-biopsy":[484],"has":[486],"been":[487],"collected.":[488],"Citation":[489],"Information:":[490],"Cancer":[491],"Prev":[492],"Res":[493],"2008;1(7":[494],"Suppl):A69.":[495]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2016-06-24T00:00:00"}