{"id":"https://openalex.org/W2018718547","doi":"https://doi.org/10.1111/j.1442-2042.1997.tb00315.x","title":"Muscarinic Receptor Subtypes and Receptor\u2010Coupled Phosphatidylinositol Hydrolysis in Rat Bladder Smooth Muscle","display_name":"Muscarinic Receptor Subtypes and Receptor\u2010Coupled Phosphatidylinositol Hydrolysis in Rat Bladder Smooth Muscle","publication_year":1997,"publication_date":"1997-11-01","ids":{"openalex":"https://openalex.org/W2018718547","doi":"https://doi.org/10.1111/j.1442-2042.1997.tb00315.x","mag":"2018718547","pmid":"https://pubmed.ncbi.nlm.nih.gov/9477190"},"language":"en","primary_location":{"id":"doi:10.1111/j.1442-2042.1997.tb00315.x","doi":"https://doi.org/10.1111/j.1442-2042.1997.tb00315.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1442-2042.1997.tb00315.x","pdf_url":null,"source":{"id":"https://openalex.org/S134892809","display_name":"International Journal of Urology","issn_l":"0919-8172","issn":["0919-8172","1442-2042"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Urology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044532292","display_name":"Hiromitsu Mimata","orcid":"https://orcid.org/0000-0002-9985-0966","observed_orcids":["https://orcid.org/0000-0002-9985-0966"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Hiromitsu Mimata","raw_affiliation_strings":["Department of Urology, Oita Medical University, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Urology, Oita Medical University, Japan","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5106131966","display_name":"Yoshio Nomura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I188815454","display_name":"Oita University","ror":"https://ror.org/01nyv7k26","country_code":"JP","type":"education","lineage":["https://openalex.org/I188815454"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yoshio Nomura","raw_affiliation_strings":["Department of Urology, Oita Medical University, Oita, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Urology, Oita Medical University, Oita, Japan","institution_ids":["https://openalex.org/I188815454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090686074","display_name":"Akio Emoto","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I188815454","display_name":"Oita University","ror":"https://ror.org/01nyv7k26","country_code":"JP","type":"education","lineage":["https://openalex.org/I188815454"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Akio Emoto","raw_affiliation_strings":["Department of Urology, Oita Medical University, Oita, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Urology, Oita Medical University, Oita, Japan","institution_ids":["https://openalex.org/I188815454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041631443","display_name":"Jamshid Latifpour","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jamshid Latifpour","raw_affiliation_strings":["Section of Urology, Department of Surgery, Yale University School of Medicine, New Haven, Connecticut, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section of Urology, Department of Surgery, Yale University School of Medicine, New Haven, Connecticut, USA","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5106973394","display_name":"Marcia A. Wheeler","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marcia Wheeler","raw_affiliation_strings":["Section of Urology, Department of Surgery, Yale University School of Medicine, New Haven, Connecticut, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section of Urology, Department of Surgery, Yale University School of Medicine, New Haven, Connecticut, USA","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"last","author":{"id":null,"display_name":"Robert M. Weiss","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert M. Weiss","raw_affiliation_strings":["Section of Urology, Department of Surgery, Yale University School of Medicine, New Haven, Connecticut, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section of Urology, Department of Surgery, Yale University School of Medicine, New Haven, Connecticut, USA","institution_ids":["https://openalex.org/I32971472"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5044532292"],"corresponding_institution_ids":[],"apc_list":{"value":3570,"currency":"USD","value_usd":3570},"apc_paid":null,"fwci":0.4714,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.66357143,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"4","issue":"6","first_page":"591","last_page":"596"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10279","display_name":"Urinary Bladder and Prostate Research","score":0.7128999829292297,"subfield":{"id":"https://openalex.org/subfields/2748","display_name":"Urology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10279","display_name":"Urinary Bladder and Prostate Research","score":0.7128999829292297,"subfield":{"id":"https://openalex.org/subfields/2748","display_name":"Urology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.26589998602867126,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11193","display_name":"Adenosine and Purinergic Signaling","score":0.0035000001080334187,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/muscarinic-receptors","display_name":"muscarinic receptors","score":0.9150000214576721},{"id":"https://openalex.org/keywords/m2-muscarinic-receptor","display_name":"M2 muscarinic receptor","score":0.9179999828338623},{"id":"https://openalex.org/keywords/muscarinic-m3-receptor","display_name":"M3 muscarinic receptor","score":1.0},{"id":"https://openalex.org/keywords/bladder-smooth-muscle","display_name":"bladder smooth muscle","score":0.859000027179718},{"id":"https://openalex.org/keywords/phosphoinositide-hydrolysis","display_name":"phosphoinositide hydrolysis","score":0.9890000224113464},{"id":"https://openalex.org/keywords/inositol-1-4-5-trisphosphate","display_name":"inositol 1,4,5-trisphosphate","score":0.9879999756813049},{"id":"https://openalex.org/keywords/carbachol","display_name":"carbachol","score":0.8460000157356262},{"id":"https://openalex.org/keywords/4-damp","display_name":"4-DAMP","score":0.906000018119812},{"id":"https://openalex.org/keywords/methoctramine","display_name":"methoctramine","score":0.9890000224113464},{"id":"https://openalex.org/keywords/pirenzepine","display_name":"pirenzepine","score":0.8659999966621399},{"id":"https://openalex.org/keywords/atropine","display_name":"atropine","score":0.9380000233650208},{"id":"https://openalex.org/keywords/smooth-muscle-contraction","display_name":"smooth muscle contraction","score":0.6949999928474426},{"id":"https://openalex.org/keywords/receptor-mrna-expression","display_name":"receptor mRNA expression","score":0.6399999856948853}],"concepts":[{"id":"https://openalex.org/C2778616617","wikidata":"https://www.wikidata.org/wiki/Q419550","display_name":"Pirenzepine","level":4,"score":0.9215456247329712},{"id":"https://openalex.org/C2776961477","wikidata":"https://www.wikidata.org/wiki/Q18344124","display_name":"Methoctramine","level":5,"score":0.8807489275932312},{"id":"https://openalex.org/C33789571","wikidata":"https://www.wikidata.org/wiki/Q410959","display_name":"Muscarinic acetylcholine receptor","level":3,"score":0.8628785610198975},{"id":"https://openalex.org/C2778988552","wikidata":"https://www.wikidata.org/wiki/Q419401","display_name":"Carbachol","level":3,"score":0.842552661895752},{"id":"https://openalex.org/C116289061","wikidata":"https://www.wikidata.org/wiki/Q17861955","display_name":"Muscarinic acetylcholine receptor M3","level":4,"score":0.7116949558258057},{"id":"https://openalex.org/C47488739","wikidata":"https://www.wikidata.org/wiki/Q17861931","display_name":"Muscarinic acetylcholine receptor M2","level":4,"score":0.6794682741165161},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.6453298926353455},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.6199326515197754},{"id":"https://openalex.org/C207723603","wikidata":"https://www.wikidata.org/wiki/Q17861926","display_name":"Muscarinic acetylcholine receptor M1","level":4,"score":0.5947666764259338},{"id":"https://openalex.org/C2780610907","wikidata":"https://www.wikidata.org/wiki/Q2273248","display_name":"Phosphatidylinositol","level":3,"score":0.5078626275062561},{"id":"https://openalex.org/C2776723954","wikidata":"https://www.wikidata.org/wiki/Q3001265","display_name":"Muscarinic antagonist","level":4,"score":0.5014815330505371},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.4936476945877075},{"id":"https://openalex.org/C2780616540","wikidata":"https://www.wikidata.org/wiki/Q568055","display_name":"Detrusor muscle","level":3,"score":0.4544012248516083},{"id":"https://openalex.org/C24998067","wikidata":"https://www.wikidata.org/wiki/Q4114622","display_name":"Stimulation","level":2,"score":0.4466608762741089},{"id":"https://openalex.org/C2778122271","wikidata":"https://www.wikidata.org/wiki/Q410943","display_name":"Receptor antagonist","level":4,"score":0.4396994113922119},{"id":"https://openalex.org/C64615621","wikidata":"https://www.wikidata.org/wiki/Q15333405","display_name":"Muscarinic acetylcholine receptor M5","level":5,"score":0.42369693517684937},{"id":"https://openalex.org/C2777427919","wikidata":"https://www.wikidata.org/wiki/Q407997","display_name":"Inositol","level":3,"score":0.42038577795028687},{"id":"https://openalex.org/C2778597717","wikidata":"https://www.wikidata.org/wiki/Q420248","display_name":"Phospholipase C","level":3,"score":0.41419869661331177},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36103537678718567},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3553324043750763},{"id":"https://openalex.org/C2776885963","wikidata":"https://www.wikidata.org/wiki/Q245204","display_name":"Antagonist","level":3,"score":0.3449735939502716},{"id":"https://openalex.org/C2779762690","wikidata":"https://www.wikidata.org/wiki/Q9382","display_name":"Urinary bladder","level":2,"score":0.2803124785423279},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1796714961528778},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.10255396366119385}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001743","descriptor_name":"Urinary Bladder","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D001743","descriptor_name":"Urinary Bladder","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009119","descriptor_name":"Muscle Contraction","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D009130","descriptor_name":"Muscle, Smooth","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D009130","descriptor_name":"Muscle, Smooth","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010716","descriptor_name":"Phosphatidylinositols","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011976","descriptor_name":"Receptors, Muscarinic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011976","descriptor_name":"Receptors, Muscarinic","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015152","descriptor_name":"Blotting, Northern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015544","descriptor_name":"Inositol 1,4,5-Trisphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017207","descriptor_name":"Rats, Sprague-Dawley","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018721","descriptor_name":"Muscarinic Agonists","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D018727","descriptor_name":"Muscarinic Antagonists","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1442-2042.1997.tb00315.x","doi":"https://doi.org/10.1111/j.1442-2042.1997.tb00315.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1442-2042.1997.tb00315.x","pdf_url":null,"source":{"id":"https://openalex.org/S134892809","display_name":"International Journal of Urology","issn_l":"0919-8172","issn":["0919-8172","1442-2042"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Urology","raw_type":"journal-article"},{"id":"pmid:9477190","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9477190","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of urology : official journal of the Japanese Urological Association","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W126880625","https://openalex.org/W1775749144","https://openalex.org/W1889236699","https://openalex.org/W1974018894","https://openalex.org/W1984004768","https://openalex.org/W1995645167","https://openalex.org/W2002494433","https://openalex.org/W2003450875","https://openalex.org/W2007469939","https://openalex.org/W2009290413","https://openalex.org/W2014669590","https://openalex.org/W2018746830","https://openalex.org/W2023430119","https://openalex.org/W2029272167","https://openalex.org/W2031241308","https://openalex.org/W2033947916","https://openalex.org/W2061184702","https://openalex.org/W2102836186","https://openalex.org/W2261468979","https://openalex.org/W2275285623","https://openalex.org/W2411059849","https://openalex.org/W2516345462"],"related_works":["https://openalex.org/W2142376219","https://openalex.org/W2022805127","https://openalex.org/W2054514092","https://openalex.org/W4299433441","https://openalex.org/W2070270781","https://openalex.org/W4247888581","https://openalex.org/W1970876703","https://openalex.org/W2152087966","https://openalex.org/W2157304637","https://openalex.org/W2798062405"],"abstract_inverted_index":{"BACKGROUND:":[0],"The":[1,135],"purpose":[2],"of":[3,34,41,43,57,65,80,102,177,185],"this":[4],"study":[5],"was":[6,140,172],"to":[7,29,106],"evaluate":[8],"the":[9,21,31,55,62,107,122,175,182],"muscarinic":[10,22,44,66],"receptor":[11,45,171],"subtypes":[12,46],"expressed":[13,91,164],"in":[14,27,54,92,165,174,188],"rat":[15,93,166,189],"bladder":[16,35,94,190],"smooth":[17,36,95,191],"muscle":[18,37,192],"and":[19,61,82,87,117,151,160],"characterize":[20],"receptor-coupled":[23],"phosphatidylinositol":[24],"(PI)":[25],"hydrolysis":[26,72],"order":[28],"clarify":[30],"first":[32],"step":[33],"contraction.":[38],"METHODS:":[39],"Expressions":[40],"mRNAs":[42,79],"were":[47,73,90],"examined":[48],"by":[49],"Northern":[50],"blot":[51],"analysis.":[52],"Changes":[53],"mass":[56],"inositol":[58],"1,4,5-trisphosphate":[59],"(IP3)":[60],"inhibitory":[63],"effects":[64],"subtype":[67],"specific":[68],"antagonists":[69],"on":[70],"PI":[71,123],"determined":[74],"after":[75,193],"carbachol":[76,194],"stimulation.":[77,195],"RESULTS:":[78],"m2":[81],"m3":[83],"genes,":[84],"encoding":[85],"M2":[86,159],"M3":[88,118,161,170],"receptors,":[89],"muscle.":[96],"Carbachol":[97],"produced":[98],"a":[99],"rapid":[100],"increase":[101],"IP3,":[103,178],"which":[104,179],"returned":[105],"basal":[108],"level":[109],"within":[110],"30":[111],"seconds.":[112],"4-Diphenylacetoxyl-N-methylpiperidine":[113],"methiodide":[114],"(4-DAMP;":[115],"M1":[116],"antagonist)":[119,128],"strongly":[120],"inhibited":[121,130],"hydrolysis,":[124],"but":[125],"methoctramine":[126],"(M2":[127],"partially":[129],"it":[131],"at":[132],"10(-4)":[133],"mol/L.":[134,157],"IC50":[136],"value":[137],"for":[138,145,152],"atropine":[139],"9.5":[141],"x":[142,148,155],"10(-9)":[143],"mol/L,":[144,150],"pirenzepine":[146],"6.4":[147],"10(-6)":[149],"4-DAMP":[153],"1.5":[154],"10(-7)":[156],"CONCLUSION:":[158],"receptors":[162],"are":[163],"urinary":[167],"bladder.":[168],"Only":[169],"involved":[173],"production":[176],"might":[180],"induce":[181],"initial":[183],"phase":[184],"contractile":[186],"response":[187]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2014,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}