{"id":"https://openalex.org/W2018602240","doi":"https://doi.org/10.1021/bi00317a024","title":"Cryoenzymology of penicillopepsin. Appendix: Mechanism of Action of Aspartyl Proteinases","display_name":"Cryoenzymology of penicillopepsin. Appendix: Mechanism of Action of Aspartyl Proteinases","publication_year":1984,"publication_date":"1984-10-01","ids":{"openalex":"https://openalex.org/W2018602240","doi":"https://doi.org/10.1021/bi00317a024","mag":"2018602240","pmid":"https://pubmed.ncbi.nlm.nih.gov/6391539"},"language":"en","primary_location":{"id":"doi:10.1021/bi00317a024","doi":"https://doi.org/10.1021/bi00317a024","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00317a024","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109108737","display_name":"Theo Hofmann","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Theo Hofmann","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113741811","display_name":"Anthony L. Fink","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Anthony L. Fink","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5009470180","display_name":"Ben M. Dunn","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ben M. Dunn","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.0628,"has_fulltext":false,"cited_by_count":38,"citation_normalized_percentile":{"value":0.75373798,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"23","issue":"22","first_page":"5247","last_page":"5256"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.7148000001907349,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.7148000001907349,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.08619999885559082,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.07649999856948853,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/penicillopepsin","display_name":"penicillopepsin","score":1.0},{"id":"https://openalex.org/keywords/aspartic-protease","display_name":"aspartic protease","score":0.9639999866485596},{"id":"https://openalex.org/keywords/enzyme-mechanism","display_name":"enzyme mechanism","score":0.5619999766349792},{"id":"https://openalex.org/keywords/covalent-catalysis","display_name":"covalent catalysis","score":0.8579999804496765},{"id":"https://openalex.org/keywords/peptide-bond-hydrolysis","display_name":"peptide bond hydrolysis","score":0.8360000252723694},{"id":"https://openalex.org/keywords/methanol","display_name":"methanol","score":0.4009999930858612},{"id":"https://openalex.org/keywords/dimethyl-sulfoxide","display_name":"dimethyl sulfoxide","score":1.0},{"id":"https://openalex.org/keywords/subzero-temperatures","display_name":"subzero temperatures","score":0.6790000200271606},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5450000166893005},{"id":"https://openalex.org/keywords/optimal-ph","display_name":"pH optimum","score":0.5609999895095825},{"id":"https://openalex.org/keywords/covalent-intermediate","display_name":"covalent intermediate","score":0.7110000252723694}],"concepts":[{"id":"https://openalex.org/C89611455","wikidata":"https://www.wikidata.org/wiki/Q6804646","display_name":"Mechanism (biology)","level":2,"score":0.5362398624420166},{"id":"https://openalex.org/C2776364125","wikidata":"https://www.wikidata.org/wiki/Q9656","display_name":"Appendix","level":2,"score":0.5214875936508179},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4985048770904541},{"id":"https://openalex.org/C2776120743","wikidata":"https://www.wikidata.org/wiki/Q3271540","display_name":"Mechanism of action","level":3,"score":0.49802184104919434},{"id":"https://openalex.org/C2780791683","wikidata":"https://www.wikidata.org/wiki/Q846785","display_name":"Action (physics)","level":2,"score":0.4193708896636963},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3809496760368347},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3437168300151825},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.19301870465278625},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.17022114992141724},{"id":"https://openalex.org/C111472728","wikidata":"https://www.wikidata.org/wiki/Q9471","display_name":"Epistemology","level":1,"score":0.14856383204460144},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.11867165565490723},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000432","descriptor_name":"Methanol","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002942","descriptor_name":"Circular Dichroism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004121","descriptor_name":"Dimethyl Sulfoxide","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005615","descriptor_name":"Freezing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011489","descriptor_name":"Protein Denaturation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016282","descriptor_name":"Aspartic Acid Endopeptidases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00317a024","doi":"https://doi.org/10.1021/bi00317a024","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00317a024","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:6391539","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6391539","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2045412484","https://openalex.org/W1544596892","https://openalex.org/W2069570015","https://openalex.org/W1980102609","https://openalex.org/W2412536257","https://openalex.org/W2093561963","https://openalex.org/W1974170314","https://openalex.org/W2010724495","https://openalex.org/W2272383432","https://openalex.org/W2034038760"],"abstract_inverted_index":{"Intrinsic":[0],"spectral":[1],"and":[2,7,22,49,140,168,182],"kinetic":[3],"properties":[4],"of":[5,42,47,56,76,82,134,147,154,180,183,225,239,274],"penicillopepsin":[6,83,250],"its":[8],"action":[9],"on":[10,78],"N-acetylalanylalanyllysyl-p-nitrophenylalanylalanylalanine":[11],"amide":[12],"have":[13],"been":[14],"investigated":[15],"at":[16,67,253],"subzero":[17],"temperatures":[18,68],"in":[19,26,38,84,132,160,165,171,187,199,212,257,277],"aqueous":[20,161,204],"methanol":[21,88,167,176,259],"dimethyl":[23,92,173,205],"sulfoxide":[24,93],"solutions":[25],"an":[27,223],"attempt":[28],"to":[29,115,138,144,163,169,215],"find":[30],"evidence":[31,61,235],"for":[32,62,107,125,236],"or":[33,89,243],"against":[34],"a":[35,54,148],"covalent":[36,241,244,275],"mechanism":[37],"the":[39,70,79,102,108,112,130,135,145,185,188,197,200,237,272],"catalyzed":[40],"hydrolysis":[41,109],"peptide":[43],"bonds.":[44],"The":[45,74,151],"study":[46],"fluorescence":[48,81],"circular":[50],"dichroism":[51],"spectra":[52],"as":[53],"function":[55],"solvent":[57],"concentrations":[58],"gave":[59],"no":[60,209],"any":[63,97],"solvent-induced":[64],"structural":[65,99],"effects":[66],"below":[69],"thermal":[71,139],"denaturation":[72],"transition.":[73],"effect":[75],"temperature":[77],"intrinsic":[80],"either":[85,240],"60%":[86,166],"(v/v)":[87,91],"50%":[90,172],"did":[94],"not":[95,268],"indicate":[96],"temperature-induced":[98],"changes.":[100],"On":[101],"other":[103],"hand,":[104],"Arrhenius":[105],"plots":[106],"reaction":[110],"over":[111],"range":[113],"0":[114],"-50":[116],"degrees":[117,255],"C":[118,256],"showed":[119],"downward":[120],"curvature.":[121],"A":[122],"probable":[123],"explanation":[124],"this":[126],"phenomenon":[127],"is":[128],"that":[129,193],"reduction":[131],"flexibility":[133],"enzyme":[136],"due":[137],"viscosity":[141],"factors":[142],"leads":[143],"stabilization":[146],"nonproductive":[149],"conformation.":[150],"pH":[152],"optima":[153],"kcat/Km":[155,230],"are":[156],"shifted":[157],"from":[158],"5.1":[159],"solvents":[162],"5.6":[164],"6.6":[170],"sulfoxide.":[174],"Aqueous":[175],"caused":[177],"small":[178],"decreases":[179],"Km":[181,218],"Kcat;":[184],"decrease":[186,198],"latter":[189],"was":[190,208,247,251],"greater":[191],"than":[192,222],"brought":[194],"about":[195],"by":[196,220],"water":[201],"concentration.":[202],"In":[203],"sulfoxide,":[206],"there":[207],"detectable":[210],"change":[211],"kcat":[213],"up":[214],"15%,":[216,228],"but":[217],"increased":[219],"more":[221],"order":[224],"magnitude.":[226],"Above":[227],"only":[229],"could":[231],"be":[232],"measured.":[233],"No":[234],"accumulation":[238],"amino":[242],"acyl":[245],"intermediates":[246,276],"obtained":[248],"when":[249],"incubated":[252],"-70":[254],"67%":[258],"with":[260],"several":[261],"substrates.":[262],"Although":[263],"negative,":[264],"these":[265],"experiments":[266],"do":[267],"rule":[269],"out":[270],"conclusively":[271],"involvement":[273],"penicillopepsin-catalyzed":[278],"reactions.":[279]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
