{"id":"https://openalex.org/W2018570286","doi":"https://doi.org/10.1002/bit.10191","title":"Inhibition of sodium butyrate\u2010induced apoptosis in recombinant Chinese hamster ovary cells by constitutively expressing antisense RNA of caspase\u20103","display_name":"Inhibition of sodium butyrate\u2010induced apoptosis in recombinant Chinese hamster ovary cells by constitutively expressing antisense RNA of caspase\u20103","publication_year":2002,"publication_date":"2002-02-12","ids":{"openalex":"https://openalex.org/W2018570286","doi":"https://doi.org/10.1002/bit.10191","mag":"2018570286","pmid":"https://pubmed.ncbi.nlm.nih.gov/11870612"},"language":"en","primary_location":{"id":"doi:10.1002/bit.10191","doi":"https://doi.org/10.1002/bit.10191","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.10191","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046424925","display_name":"No Soo Kim","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157485424","display_name":"Korea Advanced Institute of Science and Technology","ror":"https://ror.org/05apxxy63","country_code":"KR","type":"education","lineage":["https://openalex.org/I157485424"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"No Soo Kim","raw_affiliation_strings":["Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Kusong-Dong, Yusong-Gu, Taejon 305-701, Korea","fax: 82-42-869-2610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Kusong-Dong, Yusong-Gu, Taejon 305-701, Korea","institution_ids":["https://openalex.org/I157485424"]},{"raw_affiliation_string":"fax: 82-42-869-2610","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5030163258","display_name":"Gyun Min Lee","orcid":"https://orcid.org/0000-0002-9876-7378","observed_orcids":["https://orcid.org/0000-0002-9876-7378"]},"institutions":[{"id":"https://openalex.org/I157485424","display_name":"Korea Advanced Institute of Science and Technology","ror":"https://ror.org/05apxxy63","country_code":"KR","type":"education","lineage":["https://openalex.org/I157485424"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"Gyun Min Lee","raw_affiliation_strings":["Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Kusong-Dong, Yusong-Gu, Taejon 305-701, Korea; fax: 82-42-869-2610","Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Kusong-Dong, Yusong-Gu, Taejon 305-701, Korea","fax: 82-42-869-2610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Kusong-Dong, Yusong-Gu, Taejon 305-701, Korea; fax: 82-42-869-2610","institution_ids":["https://openalex.org/I157485424"]},{"raw_affiliation_string":"Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Kusong-Dong, Yusong-Gu, Taejon 305-701, Korea","institution_ids":["https://openalex.org/I157485424"]},{"raw_affiliation_string":"fax: 82-42-869-2610","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5030163258"],"corresponding_institution_ids":["https://openalex.org/I157485424"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":1.7209,"has_fulltext":false,"cited_by_count":86,"citation_normalized_percentile":{"value":0.83220303,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"78","issue":"2","first_page":"217","last_page":"228"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sodium-butyrate","display_name":"sodium butyrate","score":0.996999979019165},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.7710000276565552},{"id":"https://openalex.org/keywords/caspase-3","display_name":"caspase-3","score":0.9879999756813049},{"id":"https://openalex.org/keywords/antisense-rna","display_name":"antisense RNA","score":0.9950000047683716},{"id":"https://openalex.org/keywords/recombinant-cho-cells","display_name":"recombinant CHO cells","score":0.9419999718666077},{"id":"https://openalex.org/keywords/cytotoxicity","display_name":"cytotoxicity","score":0.7400000095367432},{"id":"https://openalex.org/keywords/mitochondrial-membrane-depolarization","display_name":"mitochondrial depolarization","score":0.9340000152587891},{"id":"https://openalex.org/keywords/antibody-productivity","display_name":"antibody productivity","score":0.6439999938011169}],"concepts":[{"id":"https://openalex.org/C2778942008","wikidata":"https://www.wikidata.org/wiki/Q305004","display_name":"Sodium butyrate","level":3,"score":0.8971999883651733},{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.7989004850387573},{"id":"https://openalex.org/C202416437","wikidata":"https://www.wikidata.org/wiki/Q423832","display_name":"Antisense RNA","level":4,"score":0.6963934302330017},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6391150951385498},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6207848191261292},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.5416266918182373},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5399150848388672},{"id":"https://openalex.org/C143141573","wikidata":"https://www.wikidata.org/wiki/Q7450971","display_name":"Sense (electronics)","level":2,"score":0.49141228199005127},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.46544989943504333},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.4643305838108063},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.42528748512268066},{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.42134159803390503},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.41082459688186646},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.36741483211517334},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.26610928773880005},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.23169571161270142},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11213418841362},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.10831010341644287},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002087","descriptor_name":"Butyrates","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D004586","descriptor_name":"Electrophoresis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008564","descriptor_name":"Membrane Potentials","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D008928","descriptor_name":"Mitochondria","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D008928","descriptor_name":"Mitochondria","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D008956","descriptor_name":"Models, Chemical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015152","descriptor_name":"Blotting, Northern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015853","descriptor_name":"Cysteine Proteinase Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D016000","descriptor_name":"Cluster Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016372","descriptor_name":"RNA, Antisense","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D016372","descriptor_name":"RNA, Antisense","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D020169","descriptor_name":"Caspases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D020169","descriptor_name":"Caspases","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D021141","descriptor_name":"Nucleic Acid Amplification Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D053148","descriptor_name":"Caspase 3","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053938","descriptor_name":"DNA Fragmentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058610","descriptor_name":"Isobutyrates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1002/bit.10191","doi":"https://doi.org/10.1002/bit.10191","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.10191","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:11870612","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11870612","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Comparative Study"},{"id":"mag:3194263525","doi":null,"is_oa":false,"landing_page_url":"https://koasas.kaist.ac.kr/handle/10203/133282","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.5683000087738037,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W78416347","https://openalex.org/W1505340772","https://openalex.org/W1544403082","https://openalex.org/W1583480419","https://openalex.org/W1916777863","https://openalex.org/W1931140155","https://openalex.org/W1966105579","https://openalex.org/W1966233022","https://openalex.org/W1971085275","https://openalex.org/W1995519653","https://openalex.org/W1998619235","https://openalex.org/W2001953711","https://openalex.org/W2004116111","https://openalex.org/W2004789944","https://openalex.org/W2008124373","https://openalex.org/W2020913460","https://openalex.org/W2032749643","https://openalex.org/W2040499395","https://openalex.org/W2043250994","https://openalex.org/W2047182740","https://openalex.org/W2065876190","https://openalex.org/W2068347130","https://openalex.org/W2080665724","https://openalex.org/W2086806187","https://openalex.org/W2090414390","https://openalex.org/W2090913926","https://openalex.org/W2093502630","https://openalex.org/W2094389211","https://openalex.org/W2098551568","https://openalex.org/W2113999599","https://openalex.org/W2116351467","https://openalex.org/W2121599902","https://openalex.org/W2166642552"],"related_works":["https://openalex.org/W3194263525","https://openalex.org/W4245596048","https://openalex.org/W2063379481","https://openalex.org/W4238018220","https://openalex.org/W2094389211","https://openalex.org/W2793600336","https://openalex.org/W2026584088","https://openalex.org/W2108489549","https://openalex.org/W2145167446","https://openalex.org/W2785765501"],"abstract_inverted_index":{"Sodium":[0],"butyrate":[1],"(NaBu)":[2],"can":[3,18,243],"enhance":[4],"the":[5,13,28,59,117,138,146,158,172,187,199,208,216],"expression":[6,42,60,150,228],"of":[7,12,31,36,57,62,65,94,102,119,126,140,151,154,161,179,191,201,210,218,229,232,239,249],"genes":[8],"controlled":[9],"by":[10,45,168],"some":[11],"mammalian":[14],"promoters,":[15],"but":[16],"it":[17,242],"also":[19],"inhibit":[20],"cell":[21,50,127,135,177,185,193,247],"growth":[22,128,148],"and":[23,69,106,129,164,252],"induce":[24],"cellular":[25,211,259],"apoptosis.":[26],"Thus,":[27],"beneficial":[29],"effect":[30,48,56],"using":[32],"a":[33,39,79,91],"higher":[34],"concentration":[35,190],"NaBu":[37,143],"on":[38,49],"foreign":[40],"protein":[41],"is":[43],"compromised":[44],"its":[46],"cytotoxic":[47,55],"growth.":[51],"To":[52],"overcome":[53],"this":[54,83,223],"NaBu,":[58,120,202],"vector":[61],"antisense":[63,84,111,152,230],"RNA":[64,85,153,231],"caspase-3":[66,95,155,233],"was":[67,174,195],"constructed":[68],"transfected":[70,109],"to":[71,207],"recombinant":[72],"Chinese":[73],"hamster":[74],"ovary":[75],"(rCHO)":[76],"cells":[77,88,105,108,122,163,251],"producing":[78,90],"humanized":[80],"antibody.":[81],"Using":[82],"strategy,":[86],"rCHO":[87,240,250],"(B3)":[89],"low":[92],"level":[93],"proenzyme":[96],"were":[97,114],"established.":[98],"When":[99],"batch":[100],"cultures":[101],"both":[103,121],"B3":[104,162,192],"control":[107,134,184],"with":[110,133,183,258],"RNA-deficient":[112],"plasmid":[113],"performed":[115],"in":[116,198],"absence":[118],"showed":[123],"similar":[124],"profiles":[125],"antibody":[130,189,237],"production.":[131],"Compared":[132],"culture,":[136,186],"under":[137],"condition":[139],"5":[141],"mM":[142],"addition":[144],"at":[145,176],"exponential":[147],"phase,":[149],"significantly":[156],"suppressed":[157],"NaBu-induced":[159,245],"apoptosis":[160],"extended":[165],"culture":[166,173,194],"longevity":[167],">2":[169],"days":[170],"if":[171],"terminated":[175],"viability":[178],"50%.":[180],"However,":[181],"compared":[182],"final":[188],"not":[196,235],"increased":[197],"presence":[200],"which":[203],"may":[204,254],"be":[205],"due":[206],"loss":[209],"metabolic":[212],"capability":[213],"resulted":[214],"from":[215],"depolarization":[217],"mitochondrial":[219],"membrane.":[220],"Taken":[221],"together,":[222],"study":[224],"suggests":[225],"that,":[226],"although":[227],"does":[234],"improve":[236],"productivity":[238],"cells,":[241],"suppress":[244],"apoptotic":[246],"death":[248],"thereby":[253],"reduce":[255],"problems":[256],"associated":[257],"disintegration.":[260]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}