{"id":"https://openalex.org/W2018498580","doi":"https://doi.org/10.4028/www.scientific.net/amr.15-17.95","title":"Real-Time PCR Quantification of Protein Expression in Skin Equivalent Monoculture and Coculture Model","display_name":"Real-Time PCR Quantification of Protein Expression in Skin Equivalent Monoculture and Coculture Model","publication_year":2006,"publication_date":"2006-02-15","ids":{"openalex":"https://openalex.org/W2018498580","doi":"https://doi.org/10.4028/www.scientific.net/amr.15-17.95","mag":"2018498580"},"language":"en","primary_location":{"id":"doi:10.4028/www.scientific.net/amr.15-17.95","doi":"https://doi.org/10.4028/www.scientific.net/amr.15-17.95","is_oa":true,"landing_page_url":"https://doi.org/10.4028/www.scientific.net/amr.15-17.95","pdf_url":null,"source":{"id":"https://openalex.org/S67425620","display_name":"Advanced materials research","issn_l":"1022-6680","issn":["1022-6680","1662-8985"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1"],"host_organization":"https://openalex.org/P4310317839","host_organization_name":"Trans Tech Publications","host_organization_lineage":["https://openalex.org/P4310317839"],"host_organization_lineage_names":["Trans Tech Publications"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Advanced Materials Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.4028/www.scientific.net/amr.15-17.95","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112769436","display_name":"Tzu Wei Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16733864","display_name":"National Taiwan University","ror":"https://ror.org/05bqach95","country_code":"TW","type":"education","lineage":["https://openalex.org/I16733864"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Tzu Wei Wang","raw_affiliation_strings":["National Taiwan University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Taiwan University","institution_ids":["https://openalex.org/I16733864"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110030859","display_name":"Hsi Chin Wu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16733864","display_name":"National Taiwan University","ror":"https://ror.org/05bqach95","country_code":"TW","type":"education","lineage":["https://openalex.org/I16733864"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Hsi Chin Wu","raw_affiliation_strings":["National Taiwan University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Taiwan University","institution_ids":["https://openalex.org/I16733864"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011229136","display_name":"Jui\u2010Sheng Sun","orcid":"https://orcid.org/0000-0001-5142-8777","observed_orcids":["https://orcid.org/0000-0001-5142-8777"]},"institutions":[{"id":"https://openalex.org/I4387156312","display_name":"National Yang Ming University","ror":"https://ror.org/009h5ks85","country_code":"TW","type":"education","lineage":["https://openalex.org/I148366613","https://openalex.org/I4387156312"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Jui Sheng Sun","raw_affiliation_strings":["National Yang-Ming University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Yang-Ming University","institution_ids":["https://openalex.org/I4387156312"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5061246570","display_name":"Feng\u2010Huei Lin","orcid":"https://orcid.org/0000-0002-2994-6671","observed_orcids":["https://orcid.org/0000-0002-2994-6671"]},"institutions":[{"id":"https://openalex.org/I16733864","display_name":"National Taiwan University","ror":"https://ror.org/05bqach95","country_code":"TW","type":"education","lineage":["https://openalex.org/I16733864"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Feng Huei Lin","raw_affiliation_strings":["National Taiwan University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Taiwan University","institution_ids":["https://openalex.org/I16733864"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0961,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.45194599,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"15-17","issue":null,"first_page":"95","last_page":"100"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11650","display_name":"Skin and Cellular Biology Research","score":0.8148999810218811,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11650","display_name":"Skin and Cellular Biology Research","score":0.8148999810218811,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":0.08290000259876251,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.035100001841783524,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/skin-equivalents","display_name":"skin equivalents","score":0.6669999957084656},{"id":"https://openalex.org/keywords/keratinocytes","display_name":"keratinocytes","score":0.8849999904632568},{"id":"https://openalex.org/keywords/dermal-fibroblasts","display_name":"dermal fibroblasts","score":0.9990000128746033},{"id":"https://openalex.org/keywords/basement-membrane-proteins","display_name":"basement membrane proteins","score":0.9399999976158142},{"id":"https://openalex.org/keywords/laminin-5","display_name":"laminin-5","score":0.9580000042915344},{"id":"https://openalex.org/keywords/type-iv-collagen","display_name":"type IV collagen","score":0.75},{"id":"https://openalex.org/keywords/type-vii-collagen","display_name":"type VII collagen","score":0.9710000157356262},{"id":"https://openalex.org/keywords/alpha6beta4-integrin","display_name":"alpha6beta4 integrin","score":0.8820000290870667},{"id":"https://openalex.org/keywords/cell-cell-interactions","display_name":"cell-cell interactions","score":0.9120000004768372},{"id":"https://openalex.org/keywords/dermal-epidermal-junction","display_name":"basement membrane zone","score":0.7770000100135803}],"concepts":[{"id":"https://openalex.org/C2779814568","wikidata":"https://www.wikidata.org/wiki/Q58345","display_name":"Laminin","level":3,"score":0.6558745503425598},{"id":"https://openalex.org/C2776193487","wikidata":"https://www.wikidata.org/wiki/Q32846","display_name":"Basement membrane","level":2,"score":0.6381403207778931},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5893828868865967},{"id":"https://openalex.org/C195687474","wikidata":"https://www.wikidata.org/wiki/Q409715","display_name":"Integrin","level":3,"score":0.5272678136825562},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5096945762634277},{"id":"https://openalex.org/C2780381497","wikidata":"https://www.wikidata.org/wiki/Q463418","display_name":"Fibroblast","level":3,"score":0.4997076988220215},{"id":"https://openalex.org/C2778005187","wikidata":"https://www.wikidata.org/wiki/Q5262669","display_name":"Dermal fibroblast","level":4,"score":0.48855647444725037},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.47008681297302246},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4677845239639282},{"id":"https://openalex.org/C2777107211","wikidata":"https://www.wikidata.org/wiki/Q7860610","display_name":"Type IV collagen","level":4,"score":0.4366828203201294},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4314485192298889},{"id":"https://openalex.org/C2776391296","wikidata":"https://www.wikidata.org/wiki/Q7535389","display_name":"Skin equivalent","level":4,"score":0.4153885841369629},{"id":"https://openalex.org/C2777074287","wikidata":"https://www.wikidata.org/wiki/Q1473931","display_name":"Keratinocyte","level":3,"score":0.3849288821220398},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.3793824315071106},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.3408356308937073},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.327700674533844},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.21837574243545532},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.4028/www.scientific.net/amr.15-17.95","doi":"https://doi.org/10.4028/www.scientific.net/amr.15-17.95","is_oa":true,"landing_page_url":"https://doi.org/10.4028/www.scientific.net/amr.15-17.95","pdf_url":null,"source":{"id":"https://openalex.org/S67425620","display_name":"Advanced materials research","issn_l":"1022-6680","issn":["1022-6680","1662-8985"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1"],"host_organization":"https://openalex.org/P4310317839","host_organization_name":"Trans Tech Publications","host_organization_lineage":["https://openalex.org/P4310317839"],"host_organization_lineage_names":["Trans Tech Publications"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Advanced Materials Research","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.4028/www.scientific.net/amr.15-17.95","doi":"https://doi.org/10.4028/www.scientific.net/amr.15-17.95","is_oa":true,"landing_page_url":"https://doi.org/10.4028/www.scientific.net/amr.15-17.95","pdf_url":null,"source":{"id":"https://openalex.org/S67425620","display_name":"Advanced materials research","issn_l":"1022-6680","issn":["1022-6680","1662-8985"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1"],"host_organization":"https://openalex.org/P4310317839","host_organization_name":"Trans Tech Publications","host_organization_lineage":["https://openalex.org/P4310317839"],"host_organization_lineage_names":["Trans Tech Publications"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Advanced Materials Research","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.504800021648407,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":7,"referenced_works":["https://openalex.org/W1659616673","https://openalex.org/W1969482526","https://openalex.org/W2018282431","https://openalex.org/W2054594987","https://openalex.org/W2067198144","https://openalex.org/W2075285486","https://openalex.org/W2122749842"],"related_works":["https://openalex.org/W2041770999","https://openalex.org/W1976285432","https://openalex.org/W2043733449","https://openalex.org/W2051829955","https://openalex.org/W2018282431","https://openalex.org/W2170798392","https://openalex.org/W1969482526","https://openalex.org/W1982478358","https://openalex.org/W2021784932","https://openalex.org/W2000569376"],"abstract_inverted_index":{"Three-dimensional":[0],"gelatin-chondroitin":[1],"6":[2,91],"sulphate-hyanuronic":[3],"acid":[4],"biomatrix":[5],"was":[6,61,111,122,143],"used":[7],"as":[8,95,97],"the":[9,13,68,106,129,137,176,182],"scaffold":[10],"to":[11,74,164],"investigate":[12],"phenotypic":[14],"and":[15,22,76,82,92,100,148,159,191],"molecular":[16],"expression":[17,138,183],"in":[18,26,31,37,115,124,128,179],"human":[19],"keratinocytes":[20,87,116,147],"(K)":[21],"dermal":[23,70,118,149,160],"fibroblasts":[24,71,119,150,161],"(FB)":[25],"three":[27],"different":[28],"culture":[29,131],"conditions":[30],"vitro.":[32],"The":[33,48],"cells":[34,60],"were":[35,72,151,197],"cultured":[36,152],"either":[38,114],"monolayer":[39],"(K":[40],"or":[41,44,117],"FB":[42],"only)":[43],"coculture":[45,126],"(K&FB)":[46],"model.":[47],"deposition":[49],"of":[50,59,139,184],"basement":[51,167],"membrane":[52,168],"proteins":[53,186,196],"secreted":[54],"by":[55,64,187,199],"these":[56],"two":[57],"kinds":[58],"quantitatively":[62],"characterized":[63],"real-time":[65,189],"PCR.":[66],"In":[67,171],"results,":[69],"shown":[73],"synthesize":[75],"deposit":[77],"laminin":[78,98],"5,":[79],"type":[80,83,102],"IV":[81],"VII":[84],"collagen,":[85],"whereas":[86],"produced":[88],"integrin":[89,107],"alpha":[90],"beta":[93,108],"4":[94,109],"well":[96],"5":[99],"collagen":[101],"IV,":[103],"VII.":[104],"Interestingly,":[105],"subunit":[110],"not":[112],"expressed":[113],"monoculture":[120],"but":[121],"seen":[123],"organotypic":[125],"model":[127],"early":[130],"period.":[132],"Furthermore,":[133],"we":[134],"found":[135],"that":[136,157,194],"those":[140],"marker":[141],"compounds":[142],"reciprocally":[144],"regulated":[145,198],"when":[146],"together.":[153],"These":[154],"results":[155],"indicated":[156],"keratinocyes":[158],"worked":[162],"together":[163],"reconstruct":[165],"dermal-epidermal":[166],"(BM)":[169],"zone.":[170],"brief,":[172],"our":[173],"data":[174],"provide":[175],"first":[177],"time":[178],"directly":[180],"quantifying":[181],"BM":[185,195],"using":[188],"PCR,":[190],"also":[192],"demonstrate":[193],"cell-cell":[200],"interaction.":[201]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}