{"id":"https://openalex.org/W2018487187","doi":"https://doi.org/10.1093/carcin/7.1.83","title":"Quantitation of carcinogen-induced DNA damage and repair in human cells with the UVR ABC excision nuclease from Escherichia coli","display_name":"Quantitation of carcinogen-induced DNA damage and repair in human cells with the UVR ABC excision nuclease from Escherichia coli","publication_year":1986,"publication_date":"1986-01-01","ids":{"openalex":"https://openalex.org/W2018487187","doi":"https://doi.org/10.1093/carcin/7.1.83","mag":"2018487187","pmid":"https://pubmed.ncbi.nlm.nih.gov/3080255"},"language":"en","primary_location":{"id":"doi:10.1093/carcin/7.1.83","doi":"https://doi.org/10.1093/carcin/7.1.83","is_oa":false,"landing_page_url":"https://doi.org/10.1093/carcin/7.1.83","pdf_url":null,"source":{"id":"https://openalex.org/S184620562","display_name":"Carcinogenesis","issn_l":"0143-3334","issn":["0143-3334","1460-2180"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Carcinogenesis","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5055919829","display_name":"Bennett Van Houten","orcid":"https://orcid.org/0000-0002-4009-2478","observed_orcids":["https://orcid.org/0000-0002-4009-2478"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bennett Van Houten","raw_affiliation_strings":["University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences Oak Ridge, TN 37831, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences Oak Ridge, TN 37831, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091884812","display_name":"Warren E. Masker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1289243028","display_name":"Oak Ridge National Laboratory","ror":"https://ror.org/01qz5mb56","country_code":"US","type":"facility","lineage":["https://openalex.org/I1289243028","https://openalex.org/I1330989302","https://openalex.org/I39565521","https://openalex.org/I4210159294"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Warren E. Masker","raw_affiliation_strings":["Biology Division, Oak Ridge National LaboratoryOak Ridge, TN 37831, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biology Division, Oak Ridge National LaboratoryOak Ridge, TN 37831, USA","institution_ids":["https://openalex.org/I1289243028"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112821268","display_name":"William L. Carrier","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1289243028","display_name":"Oak Ridge National Laboratory","ror":"https://ror.org/01qz5mb56","country_code":"US","type":"facility","lineage":["https://openalex.org/I1289243028","https://openalex.org/I1330989302","https://openalex.org/I39565521","https://openalex.org/I4210159294"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William L. Carrier","raw_affiliation_strings":["Biology Division, Oak Ridge National Laboratory Oak Ridge, TN 37831, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biology Division, Oak Ridge National Laboratory Oak Ridge, TN 37831, USA","institution_ids":["https://openalex.org/I1289243028"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5049247762","display_name":"James D. Regan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1289243028","display_name":"Oak Ridge National Laboratory","ror":"https://ror.org/01qz5mb56","country_code":"US","type":"facility","lineage":["https://openalex.org/I1289243028","https://openalex.org/I1330989302","https://openalex.org/I39565521","https://openalex.org/I4210159294"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James D. Regan","raw_affiliation_strings":["Biology Division, Oak Ridge National Laboratory Oak Ridge, TN 37831, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biology Division, Oak Ridge National Laboratory Oak Ridge, TN 37831, USA","institution_ids":["https://openalex.org/I1289243028"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4835,"currency":"USD","value_usd":4835},"apc_paid":null,"fwci":0.1439,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.41100395,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"7","issue":"1","first_page":"83","last_page":"87"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9818999767303467,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9818999767303467,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10433","display_name":"Carcinogens and Genotoxicity Assessment","score":0.016100000590085983,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-excision-repair","display_name":"excision repair","score":0.7440000176429749},{"id":"https://openalex.org/keywords/carcinogen-induced-dna-damage","display_name":"carcinogen-induced DNA damage","score":0.7129999995231628},{"id":"https://openalex.org/keywords/benzo-a-pyrene-dna-adducts","display_name":"benzo[a]pyrene-DNA adducts","score":0.8130000233650208},{"id":"https://openalex.org/keywords/anti-bpde","display_name":"anti-BPDE","score":0.7099999785423279},{"id":"https://openalex.org/keywords/n-acetoxy-2-acetylaminofluorene","display_name":"N-acetoxy-2-acetylaminofluorene","score":0.9150000214576721},{"id":"https://openalex.org/keywords/ultraviolet-radiation","display_name":"ultraviolet radiation","score":0.5569999814033508},{"id":"https://openalex.org/keywords/xeroderma-pigmentosum","display_name":"xeroderma pigmentosum","score":0.8230000138282776},{"id":"https://openalex.org/keywords/human-fibroblasts","display_name":"human fibroblasts","score":0.9210000038146973},{"id":"https://openalex.org/keywords/pyrimidine-dimers","display_name":"pyrimidine dimers","score":0.7570000290870667}],"concepts":[{"id":"https://openalex.org/C32277403","wikidata":"https://www.wikidata.org/wiki/Q740445","display_name":"Ridge","level":2,"score":0.8533012866973877},{"id":"https://openalex.org/C2775907726","wikidata":"https://www.wikidata.org/wiki/Q714439","display_name":"Oak Ridge National Laboratory","level":2,"score":0.7255457043647766},{"id":"https://openalex.org/C2779446402","wikidata":"https://www.wikidata.org/wiki/Q4036051","display_name":"Chapel","level":2,"score":0.5348671078681946},{"id":"https://openalex.org/C161191863","wikidata":"https://www.wikidata.org/wiki/Q199655","display_name":"Library science","level":1,"score":0.5210863351821899},{"id":"https://openalex.org/C555283112","wikidata":"https://www.wikidata.org/wiki/Q1637543","display_name":"Carcinogenesis","level":3,"score":0.4684355854988098},{"id":"https://openalex.org/C95457728","wikidata":"https://www.wikidata.org/wiki/Q309","display_name":"History","level":0,"score":0.3628210425376892},{"id":"https://openalex.org/C74916050","wikidata":"https://www.wikidata.org/wiki/Q841090","display_name":"Classics","level":1,"score":0.3317566514015198},{"id":"https://openalex.org/C52119013","wikidata":"https://www.wikidata.org/wiki/Q50637","display_name":"Art history","level":1,"score":0.29442712664604187},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2832717299461365},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.20665127038955688},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.16109415888786316},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.13852572441101074},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.08110576868057251},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.06618553400039673},{"id":"https://openalex.org/C185544564","wikidata":"https://www.wikidata.org/wiki/Q81197","display_name":"Nuclear physics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001580","descriptor_name":"Benzopyrenes","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002273","descriptor_name":"Carcinogens","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004260","descriptor_name":"DNA Repair","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004706","descriptor_name":"Endodeoxyribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012867","descriptor_name":"Skin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014983","descriptor_name":"Xeroderma Pigmentosum","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015123","descriptor_name":"7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1093/carcin/7.1.83","doi":"https://doi.org/10.1093/carcin/7.1.83","is_oa":false,"landing_page_url":"https://doi.org/10.1093/carcin/7.1.83","pdf_url":null,"source":{"id":"https://openalex.org/S184620562","display_name":"Carcinogenesis","issn_l":"0143-3334","issn":["0143-3334","1460-2180"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Carcinogenesis","raw_type":"journal-article"},{"id":"pmid:3080255","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3080255","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Carcinogenesis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8199999928474426}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4009652761","display_name":null,"funder_award_id":"GM7438","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7099425286","https://openalex.org/W6955044286","https://openalex.org/W4233049965","https://openalex.org/W2033860973","https://openalex.org/W1965389910","https://openalex.org/W1968752831","https://openalex.org/W2027407621","https://openalex.org/W1973234645","https://openalex.org/W1545961360","https://openalex.org/W2060522508"],"abstract_inverted_index":{"Recent":[0],"studies":[1],"by":[2,12,100,246],"others":[3],"have":[4,58],"shown":[5],"that":[6],"the":[7,13,60,90,96,134,152,178,189,222,235,243,247,258,262,273],"endonuclease":[8,63,109],"complex":[9],"coded":[10],"for":[11,271],"uvrA,":[14],"uvrB":[15],"and":[16,48,163,186],"uvrC":[17],"genes":[18],"of":[19,32,62,82,85,95,108,116,133,167,175,177,181,188,221,232,242,261,275],"Escherichia":[20],"coli":[21],"(UVR":[22],"ABC":[23,54,124,237,264],"excision":[24,55,125,171,238,265],"nuclease)":[25],"can":[26],"incise":[27,130],"DNA":[28,69,86,97,185],"containing":[29],"a":[30,131,212,268],"variety":[31],"'bulk-type'":[33],"lesions,":[34],"such":[35],"as":[36,98],"those":[37],"resulting":[38],"from":[39,71,89,183,191],"u.v.":[40,76,118,233],"light,":[41,234],"(+/-)-7":[42],"alpha,8":[43],"beta-dihydroxy-9":[44],"alpha,10":[45],"alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene":[46],"(anti-BPDE),":[47],"N-acetoxy-2-acetylaminofluorene.":[49],"Using":[50],"partially":[51],"purified":[52,68],"UVR":[53,123,236,263],"nuclease,":[56],"we":[57],"quantitated":[59],"number":[61,81,91,107,180,274],"sensitive":[64],"sites":[65,110,244],"(ESS)":[66],"in":[67,102,267,278],"isolated":[70],"human":[72,279],"fibroblasts":[73],"treated":[74,158,228],"with":[75,113,159,229],"light":[77,119],"or":[78,120],"BPDE.":[79,121],"The":[80,106,122],"ESS/10(8)":[83],"daltons":[84],"were":[87],"calculated":[88],"average":[92],"mol.":[93],"wt.":[94],"determined":[99],"sedimentation":[101],"alkaline":[103],"sucrose":[104],"gradients.":[105],"increased":[111],"linearly":[112],"increasing":[114],"doses":[115],"either":[117],"nuclease":[126,239,266],"was":[127],"able":[128],"to":[129,169,207],"majority":[132],"BPDE-DNA":[135],"adducts.":[136],"Xeroderma":[137],"pigmentosum":[138],"fibroblasts,":[139],"complementation":[140],"group":[141],"A":[142],"(XP12BE)":[143],"had":[144],"20-25%":[145],"more":[146],"ESS":[147,182,190,202,224],"at":[148,211,216],"each":[149],"dose":[150],"than":[151],"BPDE-treated":[153],"normal":[154],"(HSBP)":[155],"cells.":[156],"Cells":[157],"4":[160],"microM":[161],"BPDE":[162],"allowed":[164],"12":[165,195],"h":[166,196,218],"incubation":[168],"perform":[170],"repair":[172],"showed":[173],"removal":[174],"60%":[176,241],"initial":[179,223],"HSBP":[184,204],"40%":[187],"XP-A":[192],"DNA.":[193,280],"Beyond":[194],"XP12BE":[197],"cells":[198,205,227],"lost":[199],"no":[200],"additional":[201],"while":[203],"continued":[206],"lose":[208],"ESS,":[209],"although":[210],"slower":[213],"rate,":[214],"until":[215],"48":[217],"only":[219],"22%":[220],"remained.":[225],"In":[226],"10":[230],"J/m2":[231],"detected":[240],"recognized":[245],"pyridimine":[248],"dimer":[249],"specific":[250],"Micrococcus":[251],"luteus":[252],"glycosylase/apyrimidinic":[253],"endonuclease.":[254],"These":[255],"results":[256],"demonstrate":[257],"potential":[259],"use":[260],"quantitative":[269],"assay":[270],"determining":[272],"carcinogen-induced":[276],"lesions":[277]},"counts_by_year":[{"year":2016,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}