{"id":"https://openalex.org/W2018366802","doi":"https://doi.org/10.1016/s1046-5928(02)00599-5","title":"Expression of recombinant human GM2-activator protein in insect cells: purification and characterization by mass spectrometry","display_name":"Expression of recombinant human GM2-activator protein in insect cells: purification and characterization by mass spectrometry","publication_year":2003,"publication_date":"2003-02-01","ids":{"openalex":"https://openalex.org/W2018366802","doi":"https://doi.org/10.1016/s1046-5928(02)00599-5","mag":"2018366802","pmid":"https://pubmed.ncbi.nlm.nih.gov/12597885"},"language":"en","primary_location":{"id":"doi:10.1016/s1046-5928(02)00599-5","doi":"https://doi.org/10.1016/s1046-5928(02)00599-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s1046-5928(02)00599-5","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5089377718","display_name":"Michaela Wendeler","orcid":"https://orcid.org/0000-0001-7503-5257","observed_orcids":["https://orcid.org/0000-0001-7503-5257"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Michaela Wendeler","raw_affiliation_strings":["Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017622868","display_name":"Thorsten Lemm","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Thorsten Lemm","raw_affiliation_strings":["Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026222460","display_name":"Judith Weisgerber","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Judith Weisgerber","raw_affiliation_strings":["Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029106145","display_name":"Joerg Hoernschemeyer","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joerg Hoernschemeyer","raw_affiliation_strings":["Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080971545","display_name":"Oliver Bartelsen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Oliver Bartelsen","raw_affiliation_strings":["Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059476996","display_name":"Ute Schepers","orcid":"https://orcid.org/0000-0002-2736-6200","observed_orcids":["https://orcid.org/0000-0002-2736-6200"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ute Schepers","raw_affiliation_strings":["Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5007309669","display_name":"Konrad Sandhoff","orcid":"https://orcid.org/0000-0003-2452-8654","observed_orcids":["https://orcid.org/0000-0003-2452-8654"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Konrad Sandhoff","raw_affiliation_strings":["Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kekul\u00e9-Institut f\u00fcr Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5007309669"],"corresponding_institution_ids":[],"apc_list":{"value":3680,"currency":"USD","value_usd":3680},"apc_paid":null,"fwci":1.0748,"has_fulltext":false,"cited_by_count":28,"citation_normalized_percentile":{"value":0.73815626,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"27","issue":"2","first_page":"259","last_page":"266"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.8330000042915344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.8330000042915344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10945","display_name":"Lysosomal Storage Disorders Research","score":0.15880000591278076,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gm2-activator-protein","display_name":"GM2 activator protein","score":0.9959999918937683},{"id":"https://openalex.org/keywords/gm2-gangliosidoses","display_name":"GM2 gangliosidoses","score":0.7749999761581421},{"id":"https://openalex.org/keywords/beta-hexosaminidase-a","display_name":"beta-hexosaminidase A","score":0.9520000219345093},{"id":"https://openalex.org/keywords/baculovirus-expression-system","display_name":"baculovirus expression system","score":0.7879999876022339},{"id":"https://openalex.org/keywords/insect-cell-expression-system","display_name":"insect cell expression","score":0.7289999723434448},{"id":"https://openalex.org/keywords/sf21-cells","display_name":"Sf21 cells","score":0.8740000128746033},{"id":"https://openalex.org/keywords/protein-glycosylation","display_name":"protein glycosylation","score":0.7059999704360962},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.5619999766349792},{"id":"https://openalex.org/keywords/recombinant-protein-secretion","display_name":"recombinant protein secretion","score":0.75},{"id":"https://openalex.org/keywords/n-glycosylation","display_name":"N-glycosylation","score":0.4699999988079071}],"concepts":[{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6795640587806702},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.6331747770309448},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.6107885837554932},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.610670268535614},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5903302431106567},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.553747296333313},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4698955714702606},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3991124927997589},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3500528931617737},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.274060994386673},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002412","descriptor_name":"Cations","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002412","descriptor_name":"Cations","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002412","descriptor_name":"Cations","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002461","descriptor_name":"Cell Line, Transformed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002461","descriptor_name":"Cell Line, Transformed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002461","descriptor_name":"Cell Line, Transformed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002845","descriptor_name":"Chromatography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002845","descriptor_name":"Chromatography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002845","descriptor_name":"Chromatography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007313","descriptor_name":"Insecta","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007313","descriptor_name":"Insecta","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007313","descriptor_name":"Insecta","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008957","descriptor_name":"Models, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008957","descriptor_name":"Models, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008957","descriptor_name":"Models, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009844","descriptor_name":"Oligosaccharides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D009844","descriptor_name":"Oligosaccharides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D009844","descriptor_name":"Oligosaccharides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015151","descriptor_name":"Immunoblotting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015151","descriptor_name":"Immunoblotting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015151","descriptor_name":"Immunoblotting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016367","descriptor_name":"Baculoviridae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016367","descriptor_name":"Baculoviridae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016367","descriptor_name":"Baculoviridae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049289","descriptor_name":"G(M2) Activator Protein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D049289","descriptor_name":"G(M2) Activator Protein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D049289","descriptor_name":"G(M2) Activator Protein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s1046-5928(02)00599-5","doi":"https://doi.org/10.1016/s1046-5928(02)00599-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s1046-5928(02)00599-5","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},{"id":"pmid:12597885","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12597885","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein expression and purification","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8048999905586243,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W1484177930","https://openalex.org/W1514204573","https://openalex.org/W1515758336","https://openalex.org/W1534074292","https://openalex.org/W1536736330","https://openalex.org/W1571395330","https://openalex.org/W1814977477","https://openalex.org/W1815075576","https://openalex.org/W1982996173","https://openalex.org/W1984560166","https://openalex.org/W1997467660","https://openalex.org/W1999637882","https://openalex.org/W2020773798","https://openalex.org/W2038274403","https://openalex.org/W2074807562","https://openalex.org/W2080871194","https://openalex.org/W2089440412","https://openalex.org/W2107227103","https://openalex.org/W2144634347","https://openalex.org/W2235859352","https://openalex.org/W2314225431","https://openalex.org/W2326860060","https://openalex.org/W2331884275","https://openalex.org/W6634254726"],"related_works":["https://openalex.org/W2350146650","https://openalex.org/W2382285667","https://openalex.org/W2347849423","https://openalex.org/W2413314792","https://openalex.org/W2401891876","https://openalex.org/W2389269354","https://openalex.org/W2351771198","https://openalex.org/W2373663781","https://openalex.org/W2355684627","https://openalex.org/W2376026187"],"abstract_inverted_index":null,"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2025-10-10T00:00:00"}