{"id":"https://openalex.org/W2018030507","doi":"https://doi.org/10.1017/s1431927611001553","title":"Thermo-Cryo-Electron Microscopy of Macromolecular Complexes","display_name":"Thermo-Cryo-Electron Microscopy of Macromolecular Complexes","publication_year":2011,"publication_date":"2011-07-01","ids":{"openalex":"https://openalex.org/W2018030507","doi":"https://doi.org/10.1017/s1431927611001553","mag":"2018030507"},"language":"en","primary_location":{"id":"doi:10.1017/s1431927611001553","doi":"https://doi.org/10.1017/s1431927611001553","is_oa":true,"landing_page_url":"https://doi.org/10.1017/s1431927611001553","pdf_url":"https://www.cambridge.org/core/services/aop-cambridge-core/content/view/5F7810F45EBEFC841496A8C82886E311/S1431927611001553a.pdf/div-class-title-thermo-cryo-electron-microscopy-of-macromolecular-complexes-div.pdf","source":{"id":"https://openalex.org/S98208149","display_name":"Microscopy and Microanalysis","issn_l":"1431-9276","issn":["1431-9276","1435-8115"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microscopy and Microanalysis","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.cambridge.org/core/services/aop-cambridge-core/content/view/5F7810F45EBEFC841496A8C82886E311/S1431927611001553a.pdf/div-class-title-thermo-cryo-electron-microscopy-of-macromolecular-complexes-div.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090969844","display_name":"N. Cheng","orcid":"https://orcid.org/0009-0009-2316-8723","observed_orcids":["https://orcid.org/0009-0009-2316-8723"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"N Cheng","raw_affiliation_strings":["National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034596312","display_name":"James F. Conway","orcid":"https://orcid.org/0000-0002-6581-4748","observed_orcids":["https://orcid.org/0000-0002-6581-4748"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Conway","raw_affiliation_strings":["University of Pittsburgh School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Pittsburgh School of Medicine","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079701449","display_name":"Giovanni Cardone A","orcid":"https://orcid.org/0000-0003-4712-1451","observed_orcids":["https://orcid.org/0000-0003-4712-1451"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"G Cardone","raw_affiliation_strings":["National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021407759","display_name":"Dennis C. Winkler","orcid":"https://orcid.org/0000-0003-1445-6554","observed_orcids":["https://orcid.org/0000-0003-1445-6554"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D Winkler","raw_affiliation_strings":["National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022467380","display_name":"Brian Firek","orcid":"https://orcid.org/0000-0001-9908-8214","observed_orcids":["https://orcid.org/0000-0001-9908-8214"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"B Firek","raw_affiliation_strings":["University of Pittsburgh"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Pittsburgh","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111590892","display_name":"Roger W. Hendrix","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R Hendrix","raw_affiliation_strings":["University of Pittsburgh"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Pittsburgh","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072521798","display_name":"Robert L. Duda","orcid":"https://orcid.org/0000-0002-9517-2731","observed_orcids":["https://orcid.org/0000-0002-9517-2731"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R Duda","raw_affiliation_strings":["University of Pittsburgh"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Pittsburgh","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5090643634","display_name":"Alasdair C. Steven","orcid":"https://orcid.org/0000-0003-4104-6497","observed_orcids":["https://orcid.org/0000-0003-4104-6497"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A Steven","raw_affiliation_strings":["National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3554,"currency":"USD","value_usd":3554},"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.1458671,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"17","issue":"S2","first_page":"136","last_page":"137"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cryo-electron-microscopy","display_name":"cryo-electron microscopy","score":0.9330000281333923},{"id":"https://openalex.org/keywords/macromolecular-complexes","display_name":"macromolecular complexes","score":0.9860000014305115},{"id":"https://openalex.org/keywords/protein-structure-determination","display_name":"protein structure determination","score":0.38100001215934753}],"concepts":[{"id":"https://openalex.org/C93877712","wikidata":"https://www.wikidata.org/wiki/Q132560","display_name":"Electron microscope","level":2,"score":0.7100290060043335},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.6690069437026978},{"id":"https://openalex.org/C20702342","wikidata":"https://www.wikidata.org/wiki/Q5190506","display_name":"Cryo-electron microscopy","level":2,"score":0.6197609901428223},{"id":"https://openalex.org/C31033462","wikidata":"https://www.wikidata.org/wiki/Q3058182","display_name":"Microanalysis","level":2,"score":0.5938160419464111},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.4926932156085968},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.4245396554470062},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.3774474561214447},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2942567765712738},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.17782065272331238},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.140980064868927},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.04283955693244934},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.033824533224105835}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1017/s1431927611001553","doi":"https://doi.org/10.1017/s1431927611001553","is_oa":true,"landing_page_url":"https://doi.org/10.1017/s1431927611001553","pdf_url":"https://www.cambridge.org/core/services/aop-cambridge-core/content/view/5F7810F45EBEFC841496A8C82886E311/S1431927611001553a.pdf/div-class-title-thermo-cryo-electron-microscopy-of-macromolecular-complexes-div.pdf","source":{"id":"https://openalex.org/S98208149","display_name":"Microscopy and Microanalysis","issn_l":"1431-9276","issn":["1431-9276","1435-8115"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microscopy and Microanalysis","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1017/s1431927611001553","doi":"https://doi.org/10.1017/s1431927611001553","is_oa":true,"landing_page_url":"https://doi.org/10.1017/s1431927611001553","pdf_url":"https://www.cambridge.org/core/services/aop-cambridge-core/content/view/5F7810F45EBEFC841496A8C82886E311/S1431927611001553a.pdf/div-class-title-thermo-cryo-electron-microscopy-of-macromolecular-complexes-div.pdf","source":{"id":"https://openalex.org/S98208149","display_name":"Microscopy and Microanalysis","issn_l":"1431-9276","issn":["1431-9276","1435-8115"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microscopy and Microanalysis","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2018030507.pdf","grobid_xml":"https://content.openalex.org/works/W2018030507.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2150041155","https://openalex.org/W2130744271","https://openalex.org/W2106176781","https://openalex.org/W2143400383","https://openalex.org/W241051324","https://openalex.org/W2321996957","https://openalex.org/W2161821663","https://openalex.org/W2118895520","https://openalex.org/W2097679716","https://openalex.org/W2125608236"],"abstract_inverted_index":{"The":[0],"forces":[1],"that":[2,54],"stabilize":[3],"multi-protein":[4],"complexes":[5,56],"and":[6,45,67,87],"drive":[7],"their":[8,21,59],"assembly":[9,27,66],"operate":[10],"on":[11],"three":[12],"levels:":[13],"(i)":[14],"the":[15,29,43,51,65,81,88,96,116],"folding":[16],"of":[17,28,69,107],"individual":[18],"subunits;":[19],"(ii)":[20],"oligomerization":[22],"into":[23,31],"functional":[24],"building-blocks;":[25],"(iii)":[26],"building-blocks":[30],"final":[32],"complete":[33],"structures.There":[34],"must":[35,47],"be":[36,48,127],"coordination":[37,46],"between":[38],"these":[39,108],"respective":[40],"levels":[41],"in":[42,114,124],"hierarchy":[44],"maintained":[49],"through":[50,99],"conformational":[52],"changes":[53,123],"many":[55],"undergo":[57],"during":[58],"reaction":[60],"cycles.We":[61],"have":[62],"been":[63],"studying":[64],"maturation":[68],"bacteriophage":[70],"HK97":[71],"capsid,":[72],"considered":[73],"as":[74],"a":[75],"tractable":[76],"model":[77],"system,":[78],"whereby":[79],"both":[80],"energetics":[82],"(by":[83,90],"differential":[84],"scanning":[85],"calorimetry)":[86],"structure":[89,125],"cryo-EM)":[91],"are":[92],"addressed.As":[93],"it":[94],"matures,":[95],"capsid":[97],"passes":[98],"five":[100],"distinct":[101],"structural":[102],"states":[103],"[1].When":[104],"heated,":[105],"several":[106],"particles":[109],"exhibit":[110],"phase":[111],"transitions":[112],"[2,3]":[113],"which":[115],"protein":[117],"shell":[118],"remains":[119],"intact":[120],"but":[121],"substantial":[122],"may":[126],"incurred":[128],"[4].":[129]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}